In this study, we investigated the effects of adenovirus-mediated transfection of PC12 cells with glucose transporter 3 after ischemic injury. The results of flow cytometry and TUNEL showed that exogenous glucose tran...In this study, we investigated the effects of adenovirus-mediated transfection of PC12 cells with glucose transporter 3 after ischemic injury. The results of flow cytometry and TUNEL showed that exogenous glucose transporter 3 significantly suppressed PC12 cell apoptosis induced by ischemic injury. The results of isotopic scintiscan and western blot assays showed that, the glucose uptake rate was significantly increased and nuclear factor kappaB expression was significantly decreased after adenovirus-mediated transfection of ischemic PC12 cells with glucose transporter 3. These results suggest that adenovirus-mediated transfection of cells with glucose transporter 3 elevates the energy metabolism of PC12 cells with ischemic injury, and inhibits cell apoptosis.展开更多
In Bangladesh, both manual and e-GP system is used to perform public procurement. Before tender publication, tender documents have to be prepared by the Procurement Entities (PEs). The problem is that the manual tende...In Bangladesh, both manual and e-GP system is used to perform public procurement. Before tender publication, tender documents have to be prepared by the Procurement Entities (PEs). The problem is that the manual tendering system involves more cost and time in preparing tender documents. The study’s purpose is to compare now the efficiency of tender document preparation in the manual system with the e-GP system. This study tests the cost and time involved in tender document preparation, both manual and e-procurement tenders. Sample data was collected from the 11 RHD zones. Structured survey questionnaires were used to collect primary data from the PE officers of RHD. A hypothesis test was performed using the model of independent samples t-test. The test results indicated that e-Procurement tender document preparation costs and time were less than manual tendering. Academicians, researchers, PE officers, and policymakers will benefit from the study’s conclusions.展开更多
基金funded by the National Natural Science Foundation of China,No. 30770765
文摘In this study, we investigated the effects of adenovirus-mediated transfection of PC12 cells with glucose transporter 3 after ischemic injury. The results of flow cytometry and TUNEL showed that exogenous glucose transporter 3 significantly suppressed PC12 cell apoptosis induced by ischemic injury. The results of isotopic scintiscan and western blot assays showed that, the glucose uptake rate was significantly increased and nuclear factor kappaB expression was significantly decreased after adenovirus-mediated transfection of ischemic PC12 cells with glucose transporter 3. These results suggest that adenovirus-mediated transfection of cells with glucose transporter 3 elevates the energy metabolism of PC12 cells with ischemic injury, and inhibits cell apoptosis.
文摘In Bangladesh, both manual and e-GP system is used to perform public procurement. Before tender publication, tender documents have to be prepared by the Procurement Entities (PEs). The problem is that the manual tendering system involves more cost and time in preparing tender documents. The study’s purpose is to compare now the efficiency of tender document preparation in the manual system with the e-GP system. This study tests the cost and time involved in tender document preparation, both manual and e-procurement tenders. Sample data was collected from the 11 RHD zones. Structured survey questionnaires were used to collect primary data from the PE officers of RHD. A hypothesis test was performed using the model of independent samples t-test. The test results indicated that e-Procurement tender document preparation costs and time were less than manual tendering. Academicians, researchers, PE officers, and policymakers will benefit from the study’s conclusions.
