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Comparative Analysis of Leaf Trichomes, Epidermal Wax And Defense Enzymes Activities in Response to Puccinia horiana in Chrysanthemum and Ajania Species 被引量:10
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作者 Yinjie Wang Jun Zeng +9 位作者 Xiaolong Xia Yang Xu Jing Sun Jing Gu Hainan Sun Haini Lei Fadi Chen Jiafu Jiang Weiming Fang Sumei Chen 《Horticultural Plant Journal》 SCIE 2020年第3期191-198,共8页
White rust caused by Puccinia horiana is a destructive disease of chrysanthemum plants.To better understand the resistance mechanisms of composite species to this disease,the leaf cuticular traits,antioxidant and defe... White rust caused by Puccinia horiana is a destructive disease of chrysanthemum plants.To better understand the resistance mechanisms of composite species to this disease,the leaf cuticular traits,antioxidant and defensive enzymes activities of immune(Chrysanthemum makinoi var.wakasaense)and highly susceptible(Ajania shiwogiku var.kinokuniense)species were compared.Trichome density of two species was markedly different,negatively associated with plant resistance to P.horiana.Total wax load in C.makinoi var.wakasaense was two times more than that in A.shiwogiku var.kinokuniense.The wax composition in immune one was abundant in esters and primary alcohols.Superoxide dismutase(SOD,EC 1.15.1.1),peroxidase(POD,EC 1.11.1.7),polyphenoloxidase(PPO,EC 1.14.18.1 or EC 1.10.3.2)and phenylalanine ammonia lyase(PAL,EC 4.3.1.5)activitieswere investigated.In C.makinoi var.wakasaense,the activity of SOD and POD increased rapidly after inoculation,whichmight be non-host induced reactive oxygen species(ROS)activated antioxidant enzymes,however SOD and POD remained a low and steady level in the highly susceptible one after inoculation.Quick increase in PPO activities after inoculation was observed in both species,however it remained higher in C.makinoi var.wakasaense at the late period of inoculation.PAL in C.makinoi var.wakasaense was induced after pathogen inoculation,but not in A.shiwogiku var.kinokuniense,suggesting that these two enzymes might contribute to the resistance to P.horiana. 展开更多
关键词 CHRYSANTHEMUM puccinia horiana Resistance Leaf cuticular wax Enzyme activity
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菊花白锈病病原菌侵染循环的显微观察 被引量:4
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作者 祝朋芳 齐丹 +4 位作者 段玉玺 毛洪玉 付印东 杨迎东 屈连伟 《沈阳农业大学学报》 CAS CSCD 北大核心 2010年第2期221-223,共3页
研究了光学显微镜下菊花白锈病病原菌侵染菊花的过程。结果表明:病原菌冬孢子萌发后产生先菌丝,先菌丝上发育成2~4个担孢子。担孢子圆肾形,飞落到菊花叶表皮后萌发成菌丝,造成新的侵染点,菌丝成熟后发育成新的冬孢子,完成1个侵染循环... 研究了光学显微镜下菊花白锈病病原菌侵染菊花的过程。结果表明:病原菌冬孢子萌发后产生先菌丝,先菌丝上发育成2~4个担孢子。担孢子圆肾形,飞落到菊花叶表皮后萌发成菌丝,造成新的侵染点,菌丝成熟后发育成新的冬孢子,完成1个侵染循环。未见夏孢子。 展开更多
关键词 菊花白锈病 病原菌 侵染 显微
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菊花白锈病研究进展 被引量:9
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作者 王顺利 王红利 +1 位作者 雷增普 戴思兰 《北方园艺》 CAS 北大核心 2008年第1期67-70,共4页
菊花白锈病(Puccinia horiana Henn.)是菊花重要的流行性病害,也是多国检疫性病害,在经济上给切花菊生产造成重大损失,且有逐年加重的趋势。切花菊产业化生产刚刚在我国兴起,国内对菊花白锈病发生危害以及防治技术了解较少。现首次系统... 菊花白锈病(Puccinia horiana Henn.)是菊花重要的流行性病害,也是多国检疫性病害,在经济上给切花菊生产造成重大损失,且有逐年加重的趋势。切花菊产业化生产刚刚在我国兴起,国内对菊花白锈病发生危害以及防治技术了解较少。现首次系统介绍国内外菊花白锈病的分布与为害、发病规律和防治技术等方面的研究进展,为我国菊花白锈病的研究和防治提供参考。同时探讨了菊花抗病研究的发展方向和策略。 展开更多
关键词 菊花 菊花白锈病 综合防治 菊花抗性
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菊花白锈病抗性标准及人工离体接种鉴定方法的优化 被引量:2
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作者 祝朋芳 赵妮拉 +2 位作者 齐丹 刘娜 段玉玺 《中国农学通报》 CSCD 北大核心 2011年第13期149-152,共4页
为使菊花白锈病的抗性鉴定在可控条件下进行,笔者将目测观察与显微镜检方法相结合,制定了菊花白锈病6级抗性鉴定标准,并优化了人工离体接种方法。结果表明,培养瓶内茎段扦插法、培养皿内平铺叶片法均可用于菊花白锈病的离体鉴定;培养瓶... 为使菊花白锈病的抗性鉴定在可控条件下进行,笔者将目测观察与显微镜检方法相结合,制定了菊花白锈病6级抗性鉴定标准,并优化了人工离体接种方法。结果表明,培养瓶内茎段扦插法、培养皿内平铺叶片法均可用于菊花白锈病的离体鉴定;培养瓶内茎段扦插法优于培养皿内平铺叶片法,是最佳的人工离体接种鉴定方法。该优化方法是评价菊花白锈病抗性的既安全又简单的接种方法。 展开更多
关键词 菊花白锈病 堀氏菊柄锈菌 抗性标准 离体 显微镜检
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菊花白锈病菌冬孢子萌发的生物学特性 被引量:14
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作者 王顺利 刘红霞 戴思兰 《林业科学研究》 CSCD 北大核心 2006年第3期391-394,共4页
The white rust,caused by Puccinia horiana was one of the most important epidemic diseases on Chrysanthemum,and also was a quarantine action pest in the world.Trials determined the teliospores germinating biology of P.... The white rust,caused by Puccinia horiana was one of the most important epidemic diseases on Chrysanthemum,and also was a quarantine action pest in the world.Trials determined the teliospores germinating biology of P.horiana : the teliospores were germinated between 4 ℃ and 32 ℃,while the optimum temperature range was 15 ℃ to 24 ℃,especially between 18 ℃ and 21 ℃.Water was necessary for the teliospores germination.Without free water,it couldn’t germinate in 24 h,even with 100 % R.H.The propriety of pH for germination was pH 4 to 6.5, while pH 6 was the most favorite.2 % glucose solution promoted the germination of the teliospores evidently,whereas the fresh juice of chrysanthemum foliages restrained it prominently.Light didn’t influence on the germination. 展开更多
关键词 菊花白锈病 堀柄锈菌 冬孢子 萌发生物学特性
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菊花白锈病田间药剂防治试验 被引量:7
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作者 王顺利 刘红霞 戴思兰 《西北农业学报》 CAS CSCD 北大核心 2008年第1期116-119,136,共5页
菊花白锈病是菊花重要的流行性病害,也是世界检疫性病害。针对菊花白锈病选取10种保护性药剂和13种治疗性药剂进行田间试验,筛选出了效果好、成本低的粉锈宁1000倍液和甲壳素+冰醋酸1000倍液作为保护性药剂交替使用,腈菌唑8000倍+代森锰... 菊花白锈病是菊花重要的流行性病害,也是世界检疫性病害。针对菊花白锈病选取10种保护性药剂和13种治疗性药剂进行田间试验,筛选出了效果好、成本低的粉锈宁1000倍液和甲壳素+冰醋酸1000倍液作为保护性药剂交替使用,腈菌唑8000倍+代森锰锌1200倍液和黑杀3000倍液作为治疗性药剂交替使用。还针对带菌插穗进行药剂处理,提出先蘸世高4000倍液再蘸生根粉,为从种苗切断传染源提供了依据。这些结果为有效控制菊花白锈病的传播提供了保证。 展开更多
关键词 菊花 菊花白锈病 药剂防治
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Optimization on Identification Standards and Artificial Inoculation Methods In Vitro on Resistance to Chrysanthemum White Rust
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作者 祝朋芳 赵妮拉 +2 位作者 齐丹 刘娜 段玉玺 《Agricultural Science & Technology》 CAS 2011年第11期1640-1644,共5页
[Objective] This research aimed at exploring an effective way for inoculation and identification of chrysanthemum white rust under controlled conditions. [Method] By combining the observation methods with the naked ey... [Objective] This research aimed at exploring an effective way for inoculation and identification of chrysanthemum white rust under controlled conditions. [Method] By combining the observation methods with the naked eye and under optical microscope, we had established the identification standards for chrysanthemum white rust with six classifications and optimized artificial inoculation methods in vitro. [Result] The results showed that bottled cuttings identification method and petri dished leaves identification method both can be used for identification in vitro of chrysanthemum white rust, bottled cuttings identification method had shown better effects than petri dished leaves identification method, and was supposed to be best artificial inoculation and identification method in vitro. [Conclusion] This research had provided a scientific method for safe and effective researches on chrysanthemum white rust, in order to control the occurrence and diffusion of this quarantine disease. 展开更多
关键词 Chrysanthemum white rust (CWR) puccinia horiana henn Identification standards In vitro Microscopy test
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