The essential oils from Mentha viridis L. and Mentha pulegium L. were characterized by gas chromatography coupled to mass spectrometry (GC-MS). These oils were obtained by hydrodistillation and presented linalool (40....The essential oils from Mentha viridis L. and Mentha pulegium L. were characterized by gas chromatography coupled to mass spectrometry (GC-MS). These oils were obtained by hydrodistillation and presented linalool (40.70%), carvone (13.52%) and α-terpinene (8.56%) as the principal constituents in the essential oil from Mentha viridis L. Pulegone (50.01%), menthol (31.90%) and menthone (16.56%) were the principal constituents in the essential oil from Mentha pulegium L. These essential oils (in concentrations ranging from 3.91 to 500 μL·mL-1) showed satisfactory activities against Escherichia coli, Listeria monocytogenes, Salmonella choleraesuis and Staphylococcus aureus. The antioxidant activities with 2-deoxyribose and phosphomolybdenum and the reducing power (in concentrations ranging from 0.78 to 1000 μL·mL-1) were determined. The antioxidant activity was observed for the two oils evaluated by the phosphomolybdenum and 2-deoxyribose methods, whereas the essential oil from M. viridis presented low antioxidant activity in the reducing power assay.展开更多
Background:Allochrusa gypsophiloides Rgl(Caryophyllaceae)is the best known endemic saponin-bearing plant of Central Asia,being widely used in traditional medicine and different branches of industry.In traditional medi...Background:Allochrusa gypsophiloides Rgl(Caryophyllaceae)is the best known endemic saponin-bearing plant of Central Asia,being widely used in traditional medicine and different branches of industry.In traditional medicine,the decoction of the roots of this plant has been used as an expectorant for bronchitis,similar to other drugs with saponins.Methods:For the first time,the chemical composition of the volatile secondary metabolites obtained from the aerial parts of A.gypsophiloides was evaluated using GC-MS method.In vitro antioxidant activity of the essential oil was evaluated using DPPH and ABTS assays.The cytotoxic activities were carried out by MTT assay.Results:A total of 59 components were identified in this species representing 89.8%of the whole oil.The A.gypsophiloides essential oil was rich in oxygenated monoterpenoids,whereas pulegone(32.9%),trans-p-menthan-3-one(6.2%),hexadecanoic acid(3.6%),trans-verbenol(3.2%),phytol(3.2%),2-isopropyl-5-methylcyclohexanone(2.7%),isopiperitenon(2.1%)were predominant in the oil.The weak activity was found for the oil with IC50 values 456.4±5.39μg/ml for DPPH and 745.0±6.87μg/ml for ABTS assay.The essential oil showed moderate cytotoxic effects against prostate cancer(PC-3)(IC50:89.9±2.01μg/ml)and colon adenocarcinoma(HT-29)cells(IC50:43.6±2.38μg/ml).Conclusion:The cytotoxic activity of A.gypsophiloides essential oil against HT-29(colon)cancer cells is consistent with antitumor effects of some species from Allochrusa genus and their traditional use to treat cancer diseases.展开更多
基金The authors acknowledge the support of the Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq)the Fundacao de Amparo a Pesquisa do Estado de Minas Gerais(FAPEMIG) the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior(CAPES)for a PVNS fellowship(D.L.Nelson).
文摘The essential oils from Mentha viridis L. and Mentha pulegium L. were characterized by gas chromatography coupled to mass spectrometry (GC-MS). These oils were obtained by hydrodistillation and presented linalool (40.70%), carvone (13.52%) and α-terpinene (8.56%) as the principal constituents in the essential oil from Mentha viridis L. Pulegone (50.01%), menthol (31.90%) and menthone (16.56%) were the principal constituents in the essential oil from Mentha pulegium L. These essential oils (in concentrations ranging from 3.91 to 500 μL·mL-1) showed satisfactory activities against Escherichia coli, Listeria monocytogenes, Salmonella choleraesuis and Staphylococcus aureus. The antioxidant activities with 2-deoxyribose and phosphomolybdenum and the reducing power (in concentrations ranging from 0.78 to 1000 μL·mL-1) were determined. The antioxidant activity was observed for the two oils evaluated by the phosphomolybdenum and 2-deoxyribose methods, whereas the essential oil from M. viridis presented low antioxidant activity in the reducing power assay.
文摘Background:Allochrusa gypsophiloides Rgl(Caryophyllaceae)is the best known endemic saponin-bearing plant of Central Asia,being widely used in traditional medicine and different branches of industry.In traditional medicine,the decoction of the roots of this plant has been used as an expectorant for bronchitis,similar to other drugs with saponins.Methods:For the first time,the chemical composition of the volatile secondary metabolites obtained from the aerial parts of A.gypsophiloides was evaluated using GC-MS method.In vitro antioxidant activity of the essential oil was evaluated using DPPH and ABTS assays.The cytotoxic activities were carried out by MTT assay.Results:A total of 59 components were identified in this species representing 89.8%of the whole oil.The A.gypsophiloides essential oil was rich in oxygenated monoterpenoids,whereas pulegone(32.9%),trans-p-menthan-3-one(6.2%),hexadecanoic acid(3.6%),trans-verbenol(3.2%),phytol(3.2%),2-isopropyl-5-methylcyclohexanone(2.7%),isopiperitenon(2.1%)were predominant in the oil.The weak activity was found for the oil with IC50 values 456.4±5.39μg/ml for DPPH and 745.0±6.87μg/ml for ABTS assay.The essential oil showed moderate cytotoxic effects against prostate cancer(PC-3)(IC50:89.9±2.01μg/ml)and colon adenocarcinoma(HT-29)cells(IC50:43.6±2.38μg/ml).Conclusion:The cytotoxic activity of A.gypsophiloides essential oil against HT-29(colon)cancer cells is consistent with antitumor effects of some species from Allochrusa genus and their traditional use to treat cancer diseases.
