In order to study the damage mechanism of mineral dusts on the pulmonary alveolar macrophage （AM）, the changes in their death ratio, malandialdthyde （MDA） content and activities of lactate dehydrogenase （LDH） an...In order to study the damage mechanism of mineral dusts on the pulmonary alveolar macrophage （AM）, the changes in their death ratio, malandialdthyde （MDA） content and activities of lactate dehydrogenase （LDH） and superoxide dismutase （SOD） were measured, and the technique of cell culture in vitro was used to investigate the cytotoxicity of six mineral dusts （twelve crystal habits） from twelve mineral deposits. The results show that woUastonite and clinoptilolite have no AM cytotoxicity, while other fibrous and grainy mineral dusts damage pulmonary AM in various degrees. The cytotoxicity of fibrous mineral dusts was greater than that of the grainy ones, and the cytotoxicity of dusts was positively correlated with the active OH- content in dusts, but not necessarily so with its SiO2 content. The high pH values produced by dust was unfavorable for the survival of cells and the dusts with low bio-resistance were safe for cells. The content of variable valence elements in dusts might influence their cytotoxicity and the surface charge of dusts was not a stable factor for their toxicity. It is demonstrated that the shape of mineral dusts was one of the factors affecting cytotoxicity, and that the cytotoxicity of mineral dusts depends mainly on their properties.展开更多
Objective To investigate the changes on protein kinase C (PKC) activity of alveolar macrophages (AMs) in patients with idiopathic pulmonary fibrosis (IPF) Methods The PKC activity of AM in 9 healthy volunteers and ...Objective To investigate the changes on protein kinase C (PKC) activity of alveolar macrophages (AMs) in patients with idiopathic pulmonary fibrosis (IPF) Methods The PKC activity of AM in 9 healthy volunteers and 15 patients with IPF was investigated by measuring the radioactivity Results The total, cytosolic and membrane PKC activity of AM in bronchoalveolar lavage fluid (BALF) from patients with IPF were higher than those from control group ( P <0 01, P <0 05 and P <0 05, respectively) The total and the membrane associated PKC activity had a positive correlation with the number of cells in BALF ( r =0 8135, P <0 01 and r =0 5917, P <0 05), respectively Conclusion As a bypass of transmembrane signal transduction, PKC was suggested to be involved in the origination and development of IPF展开更多
基金supported by the National Natural Science Foundation of China(No:40072020)the Teaching and Research Award Program for 0utstanding Young Teachers in Higher Education Institutions of the Ministry of Educationthe Support Item of Preeminence Youth Fund,Sichuan.
文摘In order to study the damage mechanism of mineral dusts on the pulmonary alveolar macrophage （AM）, the changes in their death ratio, malandialdthyde （MDA） content and activities of lactate dehydrogenase （LDH） and superoxide dismutase （SOD） were measured, and the technique of cell culture in vitro was used to investigate the cytotoxicity of six mineral dusts （twelve crystal habits） from twelve mineral deposits. The results show that woUastonite and clinoptilolite have no AM cytotoxicity, while other fibrous and grainy mineral dusts damage pulmonary AM in various degrees. The cytotoxicity of fibrous mineral dusts was greater than that of the grainy ones, and the cytotoxicity of dusts was positively correlated with the active OH- content in dusts, but not necessarily so with its SiO2 content. The high pH values produced by dust was unfavorable for the survival of cells and the dusts with low bio-resistance were safe for cells. The content of variable valence elements in dusts might influence their cytotoxicity and the surface charge of dusts was not a stable factor for their toxicity. It is demonstrated that the shape of mineral dusts was one of the factors affecting cytotoxicity, and that the cytotoxicity of mineral dusts depends mainly on their properties.
基金ThisstudywassupportedbytheMinistryofHealth (No 982 164 )
文摘Objective To investigate the changes on protein kinase C (PKC) activity of alveolar macrophages (AMs) in patients with idiopathic pulmonary fibrosis (IPF) Methods The PKC activity of AM in 9 healthy volunteers and 15 patients with IPF was investigated by measuring the radioactivity Results The total, cytosolic and membrane PKC activity of AM in bronchoalveolar lavage fluid (BALF) from patients with IPF were higher than those from control group ( P <0 01, P <0 05 and P <0 05, respectively) The total and the membrane associated PKC activity had a positive correlation with the number of cells in BALF ( r =0 8135, P <0 01 and r =0 5917, P <0 05), respectively Conclusion As a bypass of transmembrane signal transduction, PKC was suggested to be involved in the origination and development of IPF
