The determination method of pulchinenoside B4 in compound Radix Pulsatillae granule was established in the paper. The results laid a foundation for quality control of compound Radix Pulsatillae granule.
[Objective] This study aimed to establish a new method for determination of anemoside B4 content in pulsatilla water extract. [Method] Using acetonitrile-water （28：72） as the mobile phase, the high performance liqu...[Objective] This study aimed to establish a new method for determination of anemoside B4 content in pulsatilla water extract. [Method] Using acetonitrile-water （28：72） as the mobile phase, the high performance liquid chromatography, equipped with UV detector, was used to determine the anemoside B4 content in pulsatilla water extract. [Result] In the concentration range of 300-800 μg/ml, anemoside B4 content showed a good linear relationship with peak area. The average recovery of anemoside B4 was 98.12% （n=-6; RSD=-1.37%）. [Conclusion] The established method meets the requirements by methodology, and it can be used to determine the anemoside B4 content in pulsatilla water extract.展开更多
Pulsatilla decoction is a famous traditional Chinese herbal formula for clearing heat and treating dysentery of animals or human. To elucidate its mechanism, many active components have been studied, however, the role...Pulsatilla decoction is a famous traditional Chinese herbal formula for clearing heat and treating dysentery of animals or human. To elucidate its mechanism, many active components have been studied, however, the roles of its polysaccharides still remain unclear. This study aimed to explore effects of polysaccharides from Pulsatilla decoction （PPD） on the microvascular endothelial glycocalyx （eGC）. The polysaccharides were extracted from PPD by water extraction and alcohol precipitation method. Mice were administered with PPD for 4 wk, and were then anesthetized with ether inhalation and were ifxed by cardiac perfusion with gradient concentration alcian blue solution. The jejunum was sampled and jejunal mucosa was prepared for ultrathin sections by routine method and was analyzed by transmission electron microscope. The results indicated that the eGC was observed as a strong electron-dense smooth linear margin or nonuniform conglomerates coating cell membranes, and PPD signiifcantly increased its thickness from （21.85±1.87） to （28.71±3.61） nm and improved its integrity. This study suggested that PPD may express their biological activities and protect against pathogenic factor damages by inlfuencing the eGC.展开更多
The present study investigated acute and subchronic toxicity and safety pharmacology of modified pulsatilla granules（MPG）to provide a basis for a comprehensive understanding of MPG toxicity.The results of acute toxi...The present study investigated acute and subchronic toxicity and safety pharmacology of modified pulsatilla granules（MPG）to provide a basis for a comprehensive understanding of MPG toxicity.The results of acute toxicity testing showed that the median lethal dose of MPG was more than 5 000 mg kg^-1,suggesting that MPG was considered as practically non-toxic.The subchronic toxicity study for 30 days was conducted by daily oral administration at doses of 375,750 and 1 500 mg kg^-1 in Sprague-Dawley rats.The results of subchronic toxicity study showed that the body weight and relative organ weight were not significantly changed by administration of MPG.The clinical chemistry study showed that MPG could induce kidney and liver damages.In histopathological,mild lesions in liver and kidney were also observed,suggesting that the liver and kidney might be potential target organs of MPG.In the safety pharmacology study,MPG did not exhibited any side effects to rats in cardiovascular system,respiratory system and central nervous system.These results suggested that MPG could be considered safe for veterinary use.展开更多
Two new compounds have been isolated from methanol extract of roots of Pulsatilla chinensis(Bunge) Hegel. Their structures were established as 3-oxo-23-hydroxy-lup-20(29)-en-28-oic acid (Ⅱ) and 3-0--L-arabinopyranosy...Two new compounds have been isolated from methanol extract of roots of Pulsatilla chinensis(Bunge) Hegel. Their structures were established as 3-oxo-23-hydroxy-lup-20(29)-en-28-oic acid (Ⅱ) and 3-0--L-arabinopyranosyl-3β, 23-dihydroxylup-20(29)-en-28-oic acid (Ⅲ).展开更多
Pulsatilla chinensis is a widely used traditional Chinese herb,which contains 56 types of chemical constituents,mainly including triterpenoid saponins,organic acids,coumarins and lignans.The largest portion of the ing...Pulsatilla chinensis is a widely used traditional Chinese herb,which contains 56 types of chemical constituents,mainly including triterpenoid saponins,organic acids,coumarins and lignans.The largest portion of the ingredients in Pulsatilla chinensis is the family of triterpenoid saponins,in which anemoside B4 is the major effective compound and indexing component.The main components of Pulsatilla chinensis can metabolize into a vast array of active products in vivo,which play vital roles in its biological activity.Mounting evidence reveals that Pulsatilla chinensis exerts a wide range of therapeutic activities,such as anti-cancer,immunoregulation,anti-inflammation and anti-schistosome,with fewer adverse reactions,via various signaling pathways and multiple targets.It was documented that the active ingredient of Pulsatilla chinensis can lessen the drug resistance and synergize the effects of other natural products including paclitaxel,as well as ameliorate the clinical efficacy of chemical drugs,such as adriamycin.However,Pulsatilla chinensis was also reported to be possibly the main cause of hemolysis and chronic liver injury.The efforts should be made to deeply investigate the pharmacological actions and underlying mechanisms of Pulsatilla chinensis,with a focus on the anti-cancer efficacy,and develop new drugs based on the components of Pulsatilla chinensis for future utilization in the clinical setting.展开更多
Objective:To study the cardioprotective effect of Baitouwen(Pulsatilla chinensis Regel,PR)in isoproterenol(ISO)induced heart failure in mice,and investigated the molecular targets using network pharmacological predict...Objective:To study the cardioprotective effect of Baitouwen(Pulsatilla chinensis Regel,PR)in isoproterenol(ISO)induced heart failure in mice,and investigated the molecular targets using network pharmacological prediction and experimental validation.Methods:PR were orally administered to ISO induced HF mice for two weeks.The cardiac function was analyzed by echocardiography.Hematoxylin-eosinstaining(HE)was used to evaluate the pathological changes.The PR targets for HF were predicted by bioinformatics analysis tool(BATMAN-TCM).The expression of atrial natriuretic peptide(ANP),brain natriuretic peptide(BNP),and HF related genes Phosphodiesterase(PDEs),were detected by real-time fluorescence quantitative PCR or Immunohistochemical staining in heart tissues.Results:Cardiac hypertrophy was observed after ISO treatment,and hypertrophic cardiomyocytes were arranged in disorder.Moreover,the expression of ANP and BNP gene in myocardium were upregulated(P<0.05).Treatment of HF mice with PR aqueous extract improved cardiac function characterized by enhanced ejection fraction(EF)and fraction shortening(FS).Furthermore,PR extract also downregulated the expression of ANP and BNP in the heart tissues.Arrangement of cardiomyocytes was also improved.Furthermore,administration of PR group decreased target genes PDE5 and PDE1C expression in the hearts.In addition,PR treatment also improved myocardial fiber distribution and decreased myocardial thickness in the hearts of HF mice.Conclusion:PR treatment may suppress cardiac hypertrophy and improve myocardial function in HF mice via inhibiting the predicted targets PDE5 expression.These results suggest the potential of using PR in preventing the development of HF.展开更多
The research literature,including the overview,clinical research and experimental research,about the treatment of ulcerative colitis(UC)with Pulsatilla Decoction was reviewed in this paper in order to provide referenc...The research literature,including the overview,clinical research and experimental research,about the treatment of ulcerative colitis(UC)with Pulsatilla Decoction was reviewed in this paper in order to provide reference for its development and application.展开更多
As a traditional Chinese herbal medicine, Pulsatilla Chinensis(P. Chinensis) is widely used in the clinical practice of traditional Chinese medicine(TCM). Recently, medical research has found that the natural product ...As a traditional Chinese herbal medicine, Pulsatilla Chinensis(P. Chinensis) is widely used in the clinical practice of traditional Chinese medicine(TCM). Recently, medical research has found that the natural product P. Chinensis saponins and their monomeric compounds extracted from P. Chinensis have a variety of biological activities, especially the triterpenoid saponins, which have significant and broad-spectrum anti-tumor effects. Moreover, its anti-tumor molecular mechanisms include inhibition of the growth and proliferation of tumor cells, repression of the migration, invasion, and metastasis, prevention of tumor cell cycle, regulation of cancer cell energy metabolism, anti-tumor angiogenesis, induction of cancer cell differentiation, apoptosis and autophagy, reversing cancer cell resistance, regulation a variety of related signaling pathways, and improvement of the inflammatory microenvironment of tumor cells. In the present work, we reviewed the related anticancer mechanisms of P. Chinensis saponins and their monomers in different tumors, aiming to provide a theoretical basis for the development and clinical application of new anti-tumor drugs of P. Chinensis saponins and their monomeric compounds.展开更多
To investigate saponins from the roots of Pulsatilla cernua （Thunb.） Bercht. et Opiz., two new compounds together with five known trlterpenold saponins were isolated. The structures of the two new trlterpenoid sapon...To investigate saponins from the roots of Pulsatilla cernua （Thunb.） Bercht. et Opiz., two new compounds together with five known trlterpenold saponins were isolated. The structures of the two new trlterpenoid saponins, named cernuasides A and B, were elucidated as 3-O-[β-D-xylopyranosyl（1-）2）]-[α-L-rhamnopyranosyl（1-）4）]-α-L- arablnopyranosyl hederagenin 28-O-β-D-glucopyranosyl ester （compound 1） and 3-O-[α-L-arabinopyranosyl（1→）3）]- [α-L-rhamnopyranosyl （1→）2）]-α-L-arabinopyranosyl hederagenin 28-O-β-D-glucopyranosyl ester （compound 2） by 1D, 2D-NMR techniques, ESIMS analysis, as well as chemical methods.展开更多
文摘The determination method of pulchinenoside B4 in compound Radix Pulsatillae granule was established in the paper. The results laid a foundation for quality control of compound Radix Pulsatillae granule.
基金Supported by General Program of Beijing Municipal Education Commission(KM201410020007)~~
文摘[Objective] This study aimed to establish a new method for determination of anemoside B4 content in pulsatilla water extract. [Method] Using acetonitrile-water （28：72） as the mobile phase, the high performance liquid chromatography, equipped with UV detector, was used to determine the anemoside B4 content in pulsatilla water extract. [Result] In the concentration range of 300-800 μg/ml, anemoside B4 content showed a good linear relationship with peak area. The average recovery of anemoside B4 was 98.12% （n=-6; RSD=-1.37%）. [Conclusion] The established method meets the requirements by methodology, and it can be used to determine the anemoside B4 content in pulsatilla water extract.
基金supported by grants from the National Natural Science Foundation of China (31001083)the Scientific Research Program of Beijing Municipal Commission of Education of China (KM201110020014, KZ201110020021)the Beijing Natural Science Foundation, China (6142004)
文摘Pulsatilla decoction is a famous traditional Chinese herbal formula for clearing heat and treating dysentery of animals or human. To elucidate its mechanism, many active components have been studied, however, the roles of its polysaccharides still remain unclear. This study aimed to explore effects of polysaccharides from Pulsatilla decoction （PPD） on the microvascular endothelial glycocalyx （eGC）. The polysaccharides were extracted from PPD by water extraction and alcohol precipitation method. Mice were administered with PPD for 4 wk, and were then anesthetized with ether inhalation and were ifxed by cardiac perfusion with gradient concentration alcian blue solution. The jejunum was sampled and jejunal mucosa was prepared for ultrathin sections by routine method and was analyzed by transmission electron microscope. The results indicated that the eGC was observed as a strong electron-dense smooth linear margin or nonuniform conglomerates coating cell membranes, and PPD signiifcantly increased its thickness from （21.85±1.87） to （28.71±3.61） nm and improved its integrity. This study suggested that PPD may express their biological activities and protect against pathogenic factor damages by inlfuencing the eGC.
基金supported by the National Natural Science Foundation of China (31372477)the International Cooperation Projects of Sichuan Province, China (2014HH0058, 2013HH0042)the Sichuan Youth Science and Technology Innovation Research Team for waterfowl disease prevention and control, China (2013TD0015)
文摘The present study investigated acute and subchronic toxicity and safety pharmacology of modified pulsatilla granules（MPG）to provide a basis for a comprehensive understanding of MPG toxicity.The results of acute toxicity testing showed that the median lethal dose of MPG was more than 5 000 mg kg^-1,suggesting that MPG was considered as practically non-toxic.The subchronic toxicity study for 30 days was conducted by daily oral administration at doses of 375,750 and 1 500 mg kg^-1 in Sprague-Dawley rats.The results of subchronic toxicity study showed that the body weight and relative organ weight were not significantly changed by administration of MPG.The clinical chemistry study showed that MPG could induce kidney and liver damages.In histopathological,mild lesions in liver and kidney were also observed,suggesting that the liver and kidney might be potential target organs of MPG.In the safety pharmacology study,MPG did not exhibited any side effects to rats in cardiovascular system,respiratory system and central nervous system.These results suggested that MPG could be considered safe for veterinary use.
文摘Two new compounds have been isolated from methanol extract of roots of Pulsatilla chinensis(Bunge) Hegel. Their structures were established as 3-oxo-23-hydroxy-lup-20(29)-en-28-oic acid (Ⅱ) and 3-0--L-arabinopyranosyl-3β, 23-dihydroxylup-20(29)-en-28-oic acid (Ⅲ).
基金National Natural Science Foundation of China(8157381381173598)+3 种基金Sichuan Provincial Admin⁃istration of Traditional Chinese Medicine of China(2021MS447)Excellent Talent Program of Chengdu University of Tra⁃ditional Chinese Medicine of China(YXRC2019002ZRYY1917)and Open Research Fund of the State Key Laboratory of Southwestern Chinese Medicine Resources of China(2020XSGG006)。
文摘Pulsatilla chinensis is a widely used traditional Chinese herb,which contains 56 types of chemical constituents,mainly including triterpenoid saponins,organic acids,coumarins and lignans.The largest portion of the ingredients in Pulsatilla chinensis is the family of triterpenoid saponins,in which anemoside B4 is the major effective compound and indexing component.The main components of Pulsatilla chinensis can metabolize into a vast array of active products in vivo,which play vital roles in its biological activity.Mounting evidence reveals that Pulsatilla chinensis exerts a wide range of therapeutic activities,such as anti-cancer,immunoregulation,anti-inflammation and anti-schistosome,with fewer adverse reactions,via various signaling pathways and multiple targets.It was documented that the active ingredient of Pulsatilla chinensis can lessen the drug resistance and synergize the effects of other natural products including paclitaxel,as well as ameliorate the clinical efficacy of chemical drugs,such as adriamycin.However,Pulsatilla chinensis was also reported to be possibly the main cause of hemolysis and chronic liver injury.The efforts should be made to deeply investigate the pharmacological actions and underlying mechanisms of Pulsatilla chinensis,with a focus on the anti-cancer efficacy,and develop new drugs based on the components of Pulsatilla chinensis for future utilization in the clinical setting.
文摘Objective:To study the cardioprotective effect of Baitouwen(Pulsatilla chinensis Regel,PR)in isoproterenol(ISO)induced heart failure in mice,and investigated the molecular targets using network pharmacological prediction and experimental validation.Methods:PR were orally administered to ISO induced HF mice for two weeks.The cardiac function was analyzed by echocardiography.Hematoxylin-eosinstaining(HE)was used to evaluate the pathological changes.The PR targets for HF were predicted by bioinformatics analysis tool(BATMAN-TCM).The expression of atrial natriuretic peptide(ANP),brain natriuretic peptide(BNP),and HF related genes Phosphodiesterase(PDEs),were detected by real-time fluorescence quantitative PCR or Immunohistochemical staining in heart tissues.Results:Cardiac hypertrophy was observed after ISO treatment,and hypertrophic cardiomyocytes were arranged in disorder.Moreover,the expression of ANP and BNP gene in myocardium were upregulated(P<0.05).Treatment of HF mice with PR aqueous extract improved cardiac function characterized by enhanced ejection fraction(EF)and fraction shortening(FS).Furthermore,PR extract also downregulated the expression of ANP and BNP in the heart tissues.Arrangement of cardiomyocytes was also improved.Furthermore,administration of PR group decreased target genes PDE5 and PDE1C expression in the hearts.In addition,PR treatment also improved myocardial fiber distribution and decreased myocardial thickness in the hearts of HF mice.Conclusion:PR treatment may suppress cardiac hypertrophy and improve myocardial function in HF mice via inhibiting the predicted targets PDE5 expression.These results suggest the potential of using PR in preventing the development of HF.
文摘The research literature,including the overview,clinical research and experimental research,about the treatment of ulcerative colitis(UC)with Pulsatilla Decoction was reviewed in this paper in order to provide reference for its development and application.
基金The National Natural Science Foundation of China (Grant No. 81703768 and 81803400)the Natural Science Foundation of Shandong Province (Grant No. ZR2017MH118 and ZR2018BH045)。
文摘As a traditional Chinese herbal medicine, Pulsatilla Chinensis(P. Chinensis) is widely used in the clinical practice of traditional Chinese medicine(TCM). Recently, medical research has found that the natural product P. Chinensis saponins and their monomeric compounds extracted from P. Chinensis have a variety of biological activities, especially the triterpenoid saponins, which have significant and broad-spectrum anti-tumor effects. Moreover, its anti-tumor molecular mechanisms include inhibition of the growth and proliferation of tumor cells, repression of the migration, invasion, and metastasis, prevention of tumor cell cycle, regulation of cancer cell energy metabolism, anti-tumor angiogenesis, induction of cancer cell differentiation, apoptosis and autophagy, reversing cancer cell resistance, regulation a variety of related signaling pathways, and improvement of the inflammatory microenvironment of tumor cells. In the present work, we reviewed the related anticancer mechanisms of P. Chinensis saponins and their monomers in different tumors, aiming to provide a theoretical basis for the development and clinical application of new anti-tumor drugs of P. Chinensis saponins and their monomeric compounds.
文摘To investigate saponins from the roots of Pulsatilla cernua （Thunb.） Bercht. et Opiz., two new compounds together with five known trlterpenold saponins were isolated. The structures of the two new trlterpenoid saponins, named cernuasides A and B, were elucidated as 3-O-[β-D-xylopyranosyl（1-）2）]-[α-L-rhamnopyranosyl（1-）4）]-α-L- arablnopyranosyl hederagenin 28-O-β-D-glucopyranosyl ester （compound 1） and 3-O-[α-L-arabinopyranosyl（1→）3）]- [α-L-rhamnopyranosyl （1→）2）]-α-L-arabinopyranosyl hederagenin 28-O-β-D-glucopyranosyl ester （compound 2） by 1D, 2D-NMR techniques, ESIMS analysis, as well as chemical methods.
