Taking the Qi and Yin deficiency syndrome as an example,the research method of pharmacology of syndrome management system was proposed.By means of text mining,systematic pharmacology and target analysis,to attempt to ...Taking the Qi and Yin deficiency syndrome as an example,the research method of pharmacology of syndrome management system was proposed.By means of text mining,systematic pharmacology and target analysis,to attempt to reveal the essence of the corresponding syndrome by studying the drugs and targets of Qi and Yin deficiency.Fourteen Chinese herbs treating Qi and Yin deficiency were retrieved and used more than 30 times,and 9,317 related targets were predicted.The common targets of action were 85.Topological analysis was carried out by using degree centrality,closeness centrality and betweenness centrality to confirm that estrogen receptor(ESR1),tumor necrosis factor(TNF),D(2)dopamine receptor(DRD2),vitamin D3 receptor(VDR),glucocorticoid receptor(NR3C1),acetylcholinesterase(ACHE)and endothelin-1(EDN1)were highly correlated with Qi and Yin deficiency syndrome.Through the target to find Qi and Yin deficiency syndrome corresponding to 17 categories of diseases.A new idea was provided for studying the biological essence of TCM clinical syndrome differentiation.展开更多
Objective To investigate the effects of Lycii Fructus(LF,Gou Qi Zi,枸杞子)and Salviae Miltiorrhizae Radix Ex Rhizoma(SM,Dan Shen,丹参)on the syndrome of deficiency with blood stasis in the RCS(rdy-/-,p-/-)rats with re...Objective To investigate the effects of Lycii Fructus(LF,Gou Qi Zi,枸杞子)and Salviae Miltiorrhizae Radix Ex Rhizoma(SM,Dan Shen,丹参)on the syndrome of deficiency with blood stasis in the RCS(rdy-/-,p-/-)rats with retinitis pigmentosa(RP).Methods A total of 32 RCS(rdy-/-,p-/-)rats were divided into 4 groups(equal amounts of female and male rats in each group):model group treated with 0.9%normal saline,LF group treated with LF formula granules,SM group treated with SM formula granules,and LF and SM(L·S)group treated with LF and SM formula granules.Eight RCS(rdy+/+,p+/+)rats(4 males and 4 females)were treated with 0.9%normal saline to serve as blank group.The contents of E2,PG,P-Selectin,plasma viscosity,whole blood relative index of the high shear rate and fibrinogen content in plasma,and the content of cAMP and cGMP in retinal homogenate were detected.The retina was evaluated by hematoxylin-eosin staining.Results The contents of E2,PG,P-Selectin,plasma viscosity,whole blood relative index of the high shear rate,and fibrinogen content in the plasma of L·S group significantly differed from those of model group(P<0.01),but were similar to those of blank group.The contents of cAMP and cGMP in the retinal homogenate of L·S group significantly differed from those in model group(P<0.01)but were similar to those in blank group(P>0.05).Conclusions LF and SM can effectively treat retinitis pigmentosa by ameliorating the syndrome of deficiency with blood stasis.展开更多
目的:分析扶正抑瘤方联合程序性死亡受体1(programmed cell death protein,PD-1)和配体1(programmed cell death protein ligand1,PD-L1)抑制剂对晚期非小细胞肺癌(non-small cell lung cancer,NSCLC)不同证型治疗效果。方法:对入选的13...目的:分析扶正抑瘤方联合程序性死亡受体1(programmed cell death protein,PD-1)和配体1(programmed cell death protein ligand1,PD-L1)抑制剂对晚期非小细胞肺癌(non-small cell lung cancer,NSCLC)不同证型治疗效果。方法:对入选的137例接受扶正抑瘤方联合PD-1/PD-L1抑制剂的NSCLC患者进行分型,并收集患者的临床资料,统计无进展生存期(Progression-free Survival,PFS),并通过功能状态(Karnofsky,KPS)评分和中医证候积分对患者治疗4周后功能状态和证候变化进行评估分析。结果:入选患者中肺脾气虚30例(21.74%),气虚痰湿证46例(33.33%),气血瘀滞证34例(24.64%),气阴两虚证28例(20.29%)。总体中位PFS(media Progressionfree Survival,mPFS)为6.0个月(95%CI:4.5~8.4)。其中气血瘀滞证mPFS最高为7.8个月(95%CI:5.5~10.0),气阴两虚证mPFS最低仅为4.5个月(95%CI:3.0~4.8)。不同证型之间mPFS差异有统计学意义(χ^(2)=15.527,P<0.01)。治疗4周后客观缓解率(Objective Response Rate,ORR)为23.66%,疾病控制率(Disease Control Rate,DCR)为62.6%。四个证型组的患者治疗4周期后KPS评分总改善有效率为63.36%,其中气血瘀滞证患者KPS改善率最高,为76.67%,其次为气虚痰湿72.73%;气阴两虚证患者KPS改善率最低,为35.71%。四证型组差异有统计学意义,气虚痰湿和气血瘀滞明显高于气阴两虚(P<0.01)。四个证型组的患者治疗满4周期后中医积分改善有效率为63.36%。其中肺脾气虚有效率为34.48%;气虚痰湿有效率为36.36%;气血瘀滞有效率为60%;气阴两虚有效率为25%。四个证型有效率对比差异有统计学意义,气血瘀滞证明显高于其他证型(P<0.05)。患者不良反应发生率为83.97%。主要包括疲劳,皮肤瘙痒、红肿、水泡,肺炎,肝、肾、甲状腺、心脏功能异常、消化道不良反应和骨髓抑制。结论:非小细胞肺癌主要可分为肺脾气虚证、气虚痰湿证、气血瘀滞证、气阴两虚证,扶正抑瘤方联合PD-1/PD-L1抑制剂对不同证型治疗效果不一,气阴两虚证治疗效果最差,可考虑更换方剂。展开更多
Objective:To investigate the characteristics of TCM syndromes in elderly patients with acute myeloid leukemia(AML)at the onset,and to analyze the main syndrome types and their correlation with age,percentage of bone m...Objective:To investigate the characteristics of TCM syndromes in elderly patients with acute myeloid leukemia(AML)at the onset,and to analyze the main syndrome types and their correlation with age,percentage of bone marrow blasts,and genetic prognostic stratification.Methods:A retrospective analysis was performed on 159 AML patients aged over 60 years who were diagnosed and treated in our hospital,and the clinical data were collected and analyzed statistically.Results:In 159 elderly AML patients,the main clinical symptoms were fatigue,poor appetite,conscious fever,and various hemorrhages.The main syndromes were Ying(42.01%),Wei(31.25%),Essential(14.81%)and Blood(11.69%);the syndromes are divided into Qi and Yin deficiency(32.70%),Qi and blood deficiency(30.19%),true Yin deficiency(28.03%)and Qi heat and blood(8.81%).The age difference between the two groups was statistically significant(P<0.001),and the multiple comparison results showed that the age of the Qi-blood deficiency group was younger than the true-Yin deficiency group and the Qi-Yin deficiency group;the percentage of bone marrow blasts was statistically significant in the three groups at the initial diagnosis(P<0.05),multiple comparison results showed that the percentage of bone marrow blasts in the true Yin deficiency group was higher than that in the Qi-blood deficiency group and the Qi-Yin deficiency group;the genetic prognostic stratification of the three groups was statistically significant(P<0.05),multiple comparisons.The results showed that the genetic prognosis of the true Yin deficiency group was poorer than that of the Qi and blood deficiency group and the Qi and Yin deficiency group.Conclusion:The most common clinical symptoms of elderly AML patients are fatigue,the disease is located in the bone marrow,and the disease is Ying,Wei,essence,and blood.With the increase in the patient's age,the TCM syndrome types tend to be more insufficiency of true Yin and deficiency of both Qi and Yin,and the prognosis of patients with insufficiency of true Yin is poor.It provides a directional scientific basis for the treatment of senile AML with integrated traditional Chinese and Western medicine.展开更多
文摘Taking the Qi and Yin deficiency syndrome as an example,the research method of pharmacology of syndrome management system was proposed.By means of text mining,systematic pharmacology and target analysis,to attempt to reveal the essence of the corresponding syndrome by studying the drugs and targets of Qi and Yin deficiency.Fourteen Chinese herbs treating Qi and Yin deficiency were retrieved and used more than 30 times,and 9,317 related targets were predicted.The common targets of action were 85.Topological analysis was carried out by using degree centrality,closeness centrality and betweenness centrality to confirm that estrogen receptor(ESR1),tumor necrosis factor(TNF),D(2)dopamine receptor(DRD2),vitamin D3 receptor(VDR),glucocorticoid receptor(NR3C1),acetylcholinesterase(ACHE)and endothelin-1(EDN1)were highly correlated with Qi and Yin deficiency syndrome.Through the target to find Qi and Yin deficiency syndrome corresponding to 17 categories of diseases.A new idea was provided for studying the biological essence of TCM clinical syndrome differentiation.
基金funding support from the National Natural science Foundation of China Funding Project (No. 81804150)Hunan Provincial Natural Science Funding Project (No. 2019JJ40226)+4 种基金National Key Discipline of TCM Diagnostics Foundation Funding Project (No. 2015ZYZD02)Hunan Provincial Department of Education Innovation Platform Open Funding Project (No. 16K065)Chinese Medicine Key Laboratory of Prevention and Treatment of Disease in Hunan Province (No. 2017TP1018)Hunan Engineering Technology Research Center for the Prevention and Treatment of Otorhinolaryngologic Diseases and Protection of Visual Function with Chinese Medicine (No. 2018TP2008)Changsha Science and Technology Plan Project
文摘Objective To investigate the effects of Lycii Fructus(LF,Gou Qi Zi,枸杞子)and Salviae Miltiorrhizae Radix Ex Rhizoma(SM,Dan Shen,丹参)on the syndrome of deficiency with blood stasis in the RCS(rdy-/-,p-/-)rats with retinitis pigmentosa(RP).Methods A total of 32 RCS(rdy-/-,p-/-)rats were divided into 4 groups(equal amounts of female and male rats in each group):model group treated with 0.9%normal saline,LF group treated with LF formula granules,SM group treated with SM formula granules,and LF and SM(L·S)group treated with LF and SM formula granules.Eight RCS(rdy+/+,p+/+)rats(4 males and 4 females)were treated with 0.9%normal saline to serve as blank group.The contents of E2,PG,P-Selectin,plasma viscosity,whole blood relative index of the high shear rate and fibrinogen content in plasma,and the content of cAMP and cGMP in retinal homogenate were detected.The retina was evaluated by hematoxylin-eosin staining.Results The contents of E2,PG,P-Selectin,plasma viscosity,whole blood relative index of the high shear rate,and fibrinogen content in the plasma of L·S group significantly differed from those of model group(P<0.01),but were similar to those of blank group.The contents of cAMP and cGMP in the retinal homogenate of L·S group significantly differed from those in model group(P<0.01)but were similar to those in blank group(P>0.05).Conclusions LF and SM can effectively treat retinitis pigmentosa by ameliorating the syndrome of deficiency with blood stasis.
文摘目的:分析扶正抑瘤方联合程序性死亡受体1(programmed cell death protein,PD-1)和配体1(programmed cell death protein ligand1,PD-L1)抑制剂对晚期非小细胞肺癌(non-small cell lung cancer,NSCLC)不同证型治疗效果。方法:对入选的137例接受扶正抑瘤方联合PD-1/PD-L1抑制剂的NSCLC患者进行分型,并收集患者的临床资料,统计无进展生存期(Progression-free Survival,PFS),并通过功能状态(Karnofsky,KPS)评分和中医证候积分对患者治疗4周后功能状态和证候变化进行评估分析。结果:入选患者中肺脾气虚30例(21.74%),气虚痰湿证46例(33.33%),气血瘀滞证34例(24.64%),气阴两虚证28例(20.29%)。总体中位PFS(media Progressionfree Survival,mPFS)为6.0个月(95%CI:4.5~8.4)。其中气血瘀滞证mPFS最高为7.8个月(95%CI:5.5~10.0),气阴两虚证mPFS最低仅为4.5个月(95%CI:3.0~4.8)。不同证型之间mPFS差异有统计学意义(χ^(2)=15.527,P<0.01)。治疗4周后客观缓解率(Objective Response Rate,ORR)为23.66%,疾病控制率(Disease Control Rate,DCR)为62.6%。四个证型组的患者治疗4周期后KPS评分总改善有效率为63.36%,其中气血瘀滞证患者KPS改善率最高,为76.67%,其次为气虚痰湿72.73%;气阴两虚证患者KPS改善率最低,为35.71%。四证型组差异有统计学意义,气虚痰湿和气血瘀滞明显高于气阴两虚(P<0.01)。四个证型组的患者治疗满4周期后中医积分改善有效率为63.36%。其中肺脾气虚有效率为34.48%;气虚痰湿有效率为36.36%;气血瘀滞有效率为60%;气阴两虚有效率为25%。四个证型有效率对比差异有统计学意义,气血瘀滞证明显高于其他证型(P<0.05)。患者不良反应发生率为83.97%。主要包括疲劳,皮肤瘙痒、红肿、水泡,肺炎,肝、肾、甲状腺、心脏功能异常、消化道不良反应和骨髓抑制。结论:非小细胞肺癌主要可分为肺脾气虚证、气虚痰湿证、气血瘀滞证、气阴两虚证,扶正抑瘤方联合PD-1/PD-L1抑制剂对不同证型治疗效果不一,气阴两虚证治疗效果最差,可考虑更换方剂。
文摘Objective:To investigate the characteristics of TCM syndromes in elderly patients with acute myeloid leukemia(AML)at the onset,and to analyze the main syndrome types and their correlation with age,percentage of bone marrow blasts,and genetic prognostic stratification.Methods:A retrospective analysis was performed on 159 AML patients aged over 60 years who were diagnosed and treated in our hospital,and the clinical data were collected and analyzed statistically.Results:In 159 elderly AML patients,the main clinical symptoms were fatigue,poor appetite,conscious fever,and various hemorrhages.The main syndromes were Ying(42.01%),Wei(31.25%),Essential(14.81%)and Blood(11.69%);the syndromes are divided into Qi and Yin deficiency(32.70%),Qi and blood deficiency(30.19%),true Yin deficiency(28.03%)and Qi heat and blood(8.81%).The age difference between the two groups was statistically significant(P<0.001),and the multiple comparison results showed that the age of the Qi-blood deficiency group was younger than the true-Yin deficiency group and the Qi-Yin deficiency group;the percentage of bone marrow blasts was statistically significant in the three groups at the initial diagnosis(P<0.05),multiple comparison results showed that the percentage of bone marrow blasts in the true Yin deficiency group was higher than that in the Qi-blood deficiency group and the Qi-Yin deficiency group;the genetic prognostic stratification of the three groups was statistically significant(P<0.05),multiple comparisons.The results showed that the genetic prognosis of the true Yin deficiency group was poorer than that of the Qi and blood deficiency group and the Qi and Yin deficiency group.Conclusion:The most common clinical symptoms of elderly AML patients are fatigue,the disease is located in the bone marrow,and the disease is Ying,Wei,essence,and blood.With the increase in the patient's age,the TCM syndrome types tend to be more insufficiency of true Yin and deficiency of both Qi and Yin,and the prognosis of patients with insufficiency of true Yin is poor.It provides a directional scientific basis for the treatment of senile AML with integrated traditional Chinese and Western medicine.
