目的:探讨补肾健脾活血方对过表达分泌型卷曲相关蛋白1(secreted frizzled related protein 1,SFRP1)、沉默SFRP1的UMR106细胞成骨分化及雌激素受体α(estrogen receptorα,ERα)的影响。方法:通过构建SFRP1过表达及沉默重组腺病毒载体...目的:探讨补肾健脾活血方对过表达分泌型卷曲相关蛋白1(secreted frizzled related protein 1,SFRP1)、沉默SFRP1的UMR106细胞成骨分化及雌激素受体α(estrogen receptorα,ERα)的影响。方法:通过构建SFRP1过表达及沉默重组腺病毒载体,并转染大鼠类成骨细胞系UMR106细胞,初步分为空载腺病毒组、过表达SFRP1组、沉默SFRP1组,并根据含药血清和生理盐水(空白)血清干预的不同分为6组,观察6组细胞的碱性磷酸酶(alkaline phosphatase,ALP)活性及细胞ERα蛋白表达情况。结果:含药血清干预的空载腺病毒组、SFRP1沉默组及SFRP1过表达组72 h后UMR106细胞ALP活性和ERα蛋白表达均高于空白血清干预的空载腺病毒组、SFRP1沉默组及SFRP1过表达组(P<0.05);空白血清+SFRP1沉默组的UMR106细胞ALP活性及ERα蛋白表达高于空白血清+空载腺病毒组(P<0.05),而空白血清+SFRP1过表达组的UMR106细胞ALP活性及ERα蛋白表达低于空白血清+空载腺病毒组(P<0.05)。结论:过表达SFRP1可以抑制UMR106细胞成骨分化,并下调ERα蛋白表达;沉默SFRP1和补肾健脾活血方均可促进UMR106细胞成骨分化,并上调ERα蛋白表达,且两者共同干预时作用更显著,说明补肾健脾活血方能够抑制SFRP1表达,而SFRP1并不是补肾健脾活血方调节成骨细胞代谢,提高成骨分化活性和促进ERα蛋白表达的唯一靶点,可能存在其他靶点共同促进调节成骨细胞代谢。展开更多
Gestational diabetes mellitus(GDM)is the most prevalent metabolic disorder during pregnancy.Women diagnosed with GDM have a substantially greater risk of developing type 2 diabetes within 5-10 years after delivery,and...Gestational diabetes mellitus(GDM)is the most prevalent metabolic disorder during pregnancy.Women diagnosed with GDM have a substantially greater risk of developing type 2 diabetes within 5-10 years after delivery,and the risk is increased by excess body weight.Uncontrolled hyperglycemia during pregnancy is potentially harmful to both mother and fetus,resulting in a greater need for Caesarian-section deliveries,delivery of larger infants with more excess body fat,a greater risk of infant death and stillbirth,and an elevated risk of infant hypoglycemia immediately after birth.Fortunately,engaging in physical activity prior to and during pregnancy may lower the risk of developing GDM.Pregnant women should also be advised how to safely increase their physical activity during pregnancy and the postpartum period.An initial approach to becoming more physically active can simply be to encourage women to incorporate more unstructured physical activity into daily living,both before and during pregnancy.Giving women an appropriate exercise prescription can encourage them to participate in physical activity safely and effectively throughout pregnancy to prevent and/or manage GDM.Engaging in 30 min of moderate intensity physical activity on most,if not all,days of the week has been adopted as a recommendation for all pregnant women.展开更多
目的:基于网络药理学和分子对接方法,研究益气活血方治疗深静脉血栓形成的作用机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)、中医药综...目的:基于网络药理学和分子对接方法,研究益气活血方治疗深静脉血栓形成的作用机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)、中医药综合数据库(traditional Chinese medicine integrated database,TCMID)、Pubchem和Uniprot数据库收集益气活血方的有效成分并规范出靶点,从GeneCards数据库筛选出深静脉血栓形成(deep vein thrombosis,DVT)的相关靶点,将两组靶点取交集得到药物-疾病共有靶点;利用STRING平台构建蛋白-蛋白互作网络(protein-protein interaction,PPI),通过DAVID数据库进行GO分析和KEGG通路富集分析;运用Cytoscape 3.7.2软件构建药物-成分-靶点-通路网络图;采用autodock vina 1.1.2软件对重要成分和潜在靶点进行分子对接研究。结果:获得益气活血方有效成分155个,潜在作用靶点246个,深静脉血栓形成靶点1977个。益气活血方调治深静脉血栓形成的核心活性成分为槲皮素、β-谷甾醇、异鼠李素、山柰酚等,核心靶点有信号转导及转录激活因子3(signal transduction and activator of transcription 3,STAT3)、丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)、血管内皮生长因子A(vascular endothelial growth factor A,VEGF-A)、白细胞介素6(interleukin-6,IL-6),涉及磷脂酰肌醇3’-激酶-蛋白激酶B[phosphatidylinositol 3’-kinase(PI3K)-Akt,PI3K/Akt]信号通路、丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信号通路、肿瘤坏死因子(tumor necrosis factor,TNF)信号通路、白细胞介素17(interleukin-17,IL-17)信号通路、缺氧诱导因子1(hypoxia inducible factor-1,HIF-1)信号通路等。分子对接结果显示核心活性成分与核心蛋白的结合活性较好。结论:益气活血方治疗深静脉血栓是多成分、多靶点、多通路相互作用的结果,研究结论为益气活血方的临床应用及实验研究提供基础。展开更多
文摘目的:探讨补肾健脾活血方对过表达分泌型卷曲相关蛋白1(secreted frizzled related protein 1,SFRP1)、沉默SFRP1的UMR106细胞成骨分化及雌激素受体α(estrogen receptorα,ERα)的影响。方法:通过构建SFRP1过表达及沉默重组腺病毒载体,并转染大鼠类成骨细胞系UMR106细胞,初步分为空载腺病毒组、过表达SFRP1组、沉默SFRP1组,并根据含药血清和生理盐水(空白)血清干预的不同分为6组,观察6组细胞的碱性磷酸酶(alkaline phosphatase,ALP)活性及细胞ERα蛋白表达情况。结果:含药血清干预的空载腺病毒组、SFRP1沉默组及SFRP1过表达组72 h后UMR106细胞ALP活性和ERα蛋白表达均高于空白血清干预的空载腺病毒组、SFRP1沉默组及SFRP1过表达组(P<0.05);空白血清+SFRP1沉默组的UMR106细胞ALP活性及ERα蛋白表达高于空白血清+空载腺病毒组(P<0.05),而空白血清+SFRP1过表达组的UMR106细胞ALP活性及ERα蛋白表达低于空白血清+空载腺病毒组(P<0.05)。结论:过表达SFRP1可以抑制UMR106细胞成骨分化,并下调ERα蛋白表达;沉默SFRP1和补肾健脾活血方均可促进UMR106细胞成骨分化,并上调ERα蛋白表达,且两者共同干预时作用更显著,说明补肾健脾活血方能够抑制SFRP1表达,而SFRP1并不是补肾健脾活血方调节成骨细胞代谢,提高成骨分化活性和促进ERα蛋白表达的唯一靶点,可能存在其他靶点共同促进调节成骨细胞代谢。
文摘Gestational diabetes mellitus(GDM)is the most prevalent metabolic disorder during pregnancy.Women diagnosed with GDM have a substantially greater risk of developing type 2 diabetes within 5-10 years after delivery,and the risk is increased by excess body weight.Uncontrolled hyperglycemia during pregnancy is potentially harmful to both mother and fetus,resulting in a greater need for Caesarian-section deliveries,delivery of larger infants with more excess body fat,a greater risk of infant death and stillbirth,and an elevated risk of infant hypoglycemia immediately after birth.Fortunately,engaging in physical activity prior to and during pregnancy may lower the risk of developing GDM.Pregnant women should also be advised how to safely increase their physical activity during pregnancy and the postpartum period.An initial approach to becoming more physically active can simply be to encourage women to incorporate more unstructured physical activity into daily living,both before and during pregnancy.Giving women an appropriate exercise prescription can encourage them to participate in physical activity safely and effectively throughout pregnancy to prevent and/or manage GDM.Engaging in 30 min of moderate intensity physical activity on most,if not all,days of the week has been adopted as a recommendation for all pregnant women.
文摘目的:基于网络药理学和分子对接方法,研究益气活血方治疗深静脉血栓形成的作用机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)、中医药综合数据库(traditional Chinese medicine integrated database,TCMID)、Pubchem和Uniprot数据库收集益气活血方的有效成分并规范出靶点,从GeneCards数据库筛选出深静脉血栓形成(deep vein thrombosis,DVT)的相关靶点,将两组靶点取交集得到药物-疾病共有靶点;利用STRING平台构建蛋白-蛋白互作网络(protein-protein interaction,PPI),通过DAVID数据库进行GO分析和KEGG通路富集分析;运用Cytoscape 3.7.2软件构建药物-成分-靶点-通路网络图;采用autodock vina 1.1.2软件对重要成分和潜在靶点进行分子对接研究。结果:获得益气活血方有效成分155个,潜在作用靶点246个,深静脉血栓形成靶点1977个。益气活血方调治深静脉血栓形成的核心活性成分为槲皮素、β-谷甾醇、异鼠李素、山柰酚等,核心靶点有信号转导及转录激活因子3(signal transduction and activator of transcription 3,STAT3)、丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)、血管内皮生长因子A(vascular endothelial growth factor A,VEGF-A)、白细胞介素6(interleukin-6,IL-6),涉及磷脂酰肌醇3’-激酶-蛋白激酶B[phosphatidylinositol 3’-kinase(PI3K)-Akt,PI3K/Akt]信号通路、丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信号通路、肿瘤坏死因子(tumor necrosis factor,TNF)信号通路、白细胞介素17(interleukin-17,IL-17)信号通路、缺氧诱导因子1(hypoxia inducible factor-1,HIF-1)信号通路等。分子对接结果显示核心活性成分与核心蛋白的结合活性较好。结论:益气活血方治疗深静脉血栓是多成分、多靶点、多通路相互作用的结果,研究结论为益气活血方的临床应用及实验研究提供基础。
