Qifu-Yin(QFY), a widely used formula of traditional Chinese medicine(TCM) derived from "Jingyue Quanshu", is one of the most commonly used TCM prescriptions for the clinical treatment of Alzheimer disease. T...Qifu-Yin(QFY), a widely used formula of traditional Chinese medicine(TCM) derived from "Jingyue Quanshu", is one of the most commonly used TCM prescriptions for the clinical treatment of Alzheimer disease. The role of advanced glycation end products(AGEs) and its receptor RAGE have attracted increasing attention as the pivotal role of Aβ has been questioned. The present study was designed to test the neuroprotective effects of QFY, and the possible mechanism in AGE-induced Alzheimer model rats. After injection of AGE in the CA3 area of the hippocampus, QFY(8.6, 4.3, and 2.15 g·kg–1), and a positive control drug donepezil(2 mg·kg–1) were administrated through gastric intubation to rats once daily for thirty consecutive days. Another positive control group was the AGE + anti-RAGE group, which was simultaneously injected with anti-RAGE antibody before AGE treatment. The control group, sham-operated group, as well as the AGE + anti-RAGE group received saline at the same dosage. The Morris water maze test and the step-down passive avoidance test were conducted to evaluate the cognitive function of the rats. The expression of RAGE and NF-κB were assayed by immunohistochemical staining. The levels of Aβ, TNF-α, and IL-1β in the hippocampus were measured by enzyme-linked immunosorbent assay(ELISA). The results showed that QFY could significantly attenuate the memory impairment induced by AGE, decrease the expressions of RAGE and NF-κB, and reduce the levels of Aβ, TNF-α, and IL-1β in the hippocampus in a dose-dependent manner. Also, the blockage of RAGE could significantly reduce the impairments caused by AGEs. In conclusion, QFY could attenuate AGEs-induced, Alzheimer-like pathophysiological changes. These neuroprotective effects might be related to the RAGE/NF-κB pathway and its anti-inflammatory activity.展开更多
目的:观察七福饮加减联合多奈哌齐治疗髓海不足型血管性痴呆患者临床疗效及血清超氧化物歧化酶、过氧化脂质表达影响。方法:90例髓海不足型血管性痴呆患者随机分为对照组45例(多奈哌齐片口服),观察组45例(对照组基础上配合七福饮加减),...目的:观察七福饮加减联合多奈哌齐治疗髓海不足型血管性痴呆患者临床疗效及血清超氧化物歧化酶、过氧化脂质表达影响。方法:90例髓海不足型血管性痴呆患者随机分为对照组45例(多奈哌齐片口服),观察组45例(对照组基础上配合七福饮加减),治疗前及疗程结束后运用简易智能状态量表(Mini-mental State Examination,MMSE)、日常生活自理能力评分(Activities of Daily Living,ADL)评价患者认知水平及日常生活自理能力改善情况,检测超氧化物歧化酶、过氧化脂肪治疗前后差异。结果:(1)观察组总有效率明显高于对照组,差异具有统计学意义(P<0.05)。(2)与本组治疗前比较治疗后两组患者MMSE评分、ADL评分均提高,观察组治疗后与对照组治疗后比较改善更加显著(P<0.05)。(3)与本组治疗前比较两组患者治疗后超氧化物歧化酶均升高,过氧化脂质下降,观察组治疗后与对照组治疗后比较变化更显著(P<0.05)。结论:七福饮加减联合多奈哌齐可以有效改善髓海不足型血管性痴呆患者认知障碍,提高日常生活自理能力,抑制氧化应激水平,值得临床推广。展开更多
对近20年相关文献的检索分析发现,七福饮(QFY)具有活血化瘀、健心安神之功效,能有效改善痴呆状态。现代临床研究结果表明,QFY加减方可有效治疗阿尔茨海默病(Alzheimer′s disease,AD)和血管性痴呆(vascu lar dementia,VD);实验药理学研...对近20年相关文献的检索分析发现,七福饮(QFY)具有活血化瘀、健心安神之功效,能有效改善痴呆状态。现代临床研究结果表明,QFY加减方可有效治疗阿尔茨海默病(Alzheimer′s disease,AD)和血管性痴呆(vascu lar dementia,VD);实验药理学研究发现,其可能的作用机制在于抑制神经细胞凋亡、降低脑内炎症反应、增强胆碱能神经传递和清除自由基等。对于QFY经典方,目前临床研究尚显不足,实验药理学研究缺乏深入的机制探索。该文从临床研究及实验药理学方面对QFY治疗痴呆的研究进展进行了综述。展开更多
基金supported by the Scientific Research Project of Administration of Traditional Medicine of Shanxi Province(13-ZY017)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)+2 种基金supported by grants from the Natural Science Foundation of Jiangsu Province of China(Grant No.BK2011630)the Fundamental Research Funds for the Central Universities(Program No.JKQ2011036)the Research Innovation Program Project for Graduate Students in Jiangsu Province(CXZZ13_03)
文摘Qifu-Yin(QFY), a widely used formula of traditional Chinese medicine(TCM) derived from "Jingyue Quanshu", is one of the most commonly used TCM prescriptions for the clinical treatment of Alzheimer disease. The role of advanced glycation end products(AGEs) and its receptor RAGE have attracted increasing attention as the pivotal role of Aβ has been questioned. The present study was designed to test the neuroprotective effects of QFY, and the possible mechanism in AGE-induced Alzheimer model rats. After injection of AGE in the CA3 area of the hippocampus, QFY(8.6, 4.3, and 2.15 g·kg–1), and a positive control drug donepezil(2 mg·kg–1) were administrated through gastric intubation to rats once daily for thirty consecutive days. Another positive control group was the AGE + anti-RAGE group, which was simultaneously injected with anti-RAGE antibody before AGE treatment. The control group, sham-operated group, as well as the AGE + anti-RAGE group received saline at the same dosage. The Morris water maze test and the step-down passive avoidance test were conducted to evaluate the cognitive function of the rats. The expression of RAGE and NF-κB were assayed by immunohistochemical staining. The levels of Aβ, TNF-α, and IL-1β in the hippocampus were measured by enzyme-linked immunosorbent assay(ELISA). The results showed that QFY could significantly attenuate the memory impairment induced by AGE, decrease the expressions of RAGE and NF-κB, and reduce the levels of Aβ, TNF-α, and IL-1β in the hippocampus in a dose-dependent manner. Also, the blockage of RAGE could significantly reduce the impairments caused by AGEs. In conclusion, QFY could attenuate AGEs-induced, Alzheimer-like pathophysiological changes. These neuroprotective effects might be related to the RAGE/NF-κB pathway and its anti-inflammatory activity.
文摘目的:观察七福饮加减联合多奈哌齐治疗髓海不足型血管性痴呆患者临床疗效及血清超氧化物歧化酶、过氧化脂质表达影响。方法:90例髓海不足型血管性痴呆患者随机分为对照组45例(多奈哌齐片口服),观察组45例(对照组基础上配合七福饮加减),治疗前及疗程结束后运用简易智能状态量表(Mini-mental State Examination,MMSE)、日常生活自理能力评分(Activities of Daily Living,ADL)评价患者认知水平及日常生活自理能力改善情况,检测超氧化物歧化酶、过氧化脂肪治疗前后差异。结果:(1)观察组总有效率明显高于对照组,差异具有统计学意义(P<0.05)。(2)与本组治疗前比较治疗后两组患者MMSE评分、ADL评分均提高,观察组治疗后与对照组治疗后比较改善更加显著(P<0.05)。(3)与本组治疗前比较两组患者治疗后超氧化物歧化酶均升高,过氧化脂质下降,观察组治疗后与对照组治疗后比较变化更显著(P<0.05)。结论:七福饮加减联合多奈哌齐可以有效改善髓海不足型血管性痴呆患者认知障碍,提高日常生活自理能力,抑制氧化应激水平,值得临床推广。
文摘对近20年相关文献的检索分析发现,七福饮(QFY)具有活血化瘀、健心安神之功效,能有效改善痴呆状态。现代临床研究结果表明,QFY加减方可有效治疗阿尔茨海默病(Alzheimer′s disease,AD)和血管性痴呆(vascu lar dementia,VD);实验药理学研究发现,其可能的作用机制在于抑制神经细胞凋亡、降低脑内炎症反应、增强胆碱能神经传递和清除自由基等。对于QFY经典方,目前临床研究尚显不足,实验药理学研究缺乏深入的机制探索。该文从临床研究及实验药理学方面对QFY治疗痴呆的研究进展进行了综述。
