Objective:To explore the target and signal pathway of Qingchang Huashi Decoction(QCHSD)in the treatment of ulcerative colitis(UC)by using network pharmacology,so as to explain its molecular mechanism of action in the ...Objective:To explore the target and signal pathway of Qingchang Huashi Decoction(QCHSD)in the treatment of ulcerative colitis(UC)by using network pharmacology,so as to explain its molecular mechanism of action in the treatment of UC from damp heat.Methods:TCMSP was used to screen the potential active components(OB≥30%,DL≥0.18)and target of QCHSD.The network of"potential active ingredients-target-disease"was constructed by using the database of TCMSP and GeneCards.Using the string platform,the protein protein interaction(PPI)network model was constructed to find the core target.Go and KEGG enrichment of potential targets were analyzed by R software.Results:The results of network analysis showed that quercetin,kaempferol,scutellarin and baicalein were the top four active ingredients in QCHSD.210 gene targets were found in QCHSD,4213 in UC.The key targets of QCHSD in treating UC included AKT1,IL-6,VEGFA,CASP3,etc.GO enrichment analysis showed that these gene targets mainly affected nuclear receptor,steroid receptor,cytokine receptor binding,cytokine activity,etc.KEGG enrichment analysis showed that AGE-RAGE signaling pathway,IL-17 signaling pathway and TNF were more abundant.Conclusion:This study describes the material basis and mechanism of QCHSD in the treatment of UC,which provides theoretical basis and research direction for future research.展开更多
基金National natural science foundation of China(No.81673905,81873260)National key research and development planning(No.2017YFC1700104)+2 种基金Project of science and technology department of Jiangsu province(No.BE2019769)Project for the construction of superior disciplines of universities and colleges in Jiangsu province[2018(87)]Scientific research innovative program for postgraduate students Jiangsu province(No.KYCX20_1530)。
文摘Objective:To explore the target and signal pathway of Qingchang Huashi Decoction(QCHSD)in the treatment of ulcerative colitis(UC)by using network pharmacology,so as to explain its molecular mechanism of action in the treatment of UC from damp heat.Methods:TCMSP was used to screen the potential active components(OB≥30%,DL≥0.18)and target of QCHSD.The network of"potential active ingredients-target-disease"was constructed by using the database of TCMSP and GeneCards.Using the string platform,the protein protein interaction(PPI)network model was constructed to find the core target.Go and KEGG enrichment of potential targets were analyzed by R software.Results:The results of network analysis showed that quercetin,kaempferol,scutellarin and baicalein were the top four active ingredients in QCHSD.210 gene targets were found in QCHSD,4213 in UC.The key targets of QCHSD in treating UC included AKT1,IL-6,VEGFA,CASP3,etc.GO enrichment analysis showed that these gene targets mainly affected nuclear receptor,steroid receptor,cytokine receptor binding,cytokine activity,etc.KEGG enrichment analysis showed that AGE-RAGE signaling pathway,IL-17 signaling pathway and TNF were more abundant.Conclusion:This study describes the material basis and mechanism of QCHSD in the treatment of UC,which provides theoretical basis and research direction for future research.
