Estimation of Regional Evapotranspiration in Alpine Area and Its Response to Land Use Change:A Case Study in Three-River Headwaters Region of Qinghai-Tibet Plateau,China

Three-River Headwaters （TRH） region involved in this paper refers to the source region of the Changjiang （Yangtze） River, the Huanghe （Yellow） River and the Lancang River in China. Taking the TRH region of the Qing- hai-Tibet Plateau as a case, the annual evapotranspiration （ET） model developed by Zhang et al. （2001） was applied to evaluate mean annual ET in the alpine area, and the response of annual ET to land use change was analyzed. The plant-available water coefficient （w） of Zhang＇s model was revised by using vegetation-temperature condition index （VTCI） before annual ET was calculated in alpine area. The future land use scenario, an input of ET model, was spa- tially simulated by using the conversion of land use and its effects at small regional extent （CLUE-S） to study the re- sponse of ET to land use change. Results show that the relative errors between the simulated ET and that calculated by using water balance equation were 3.81% and the index of agreement was 0.69. This indicates that Zhang＇s ET model based on revised plant-available water coefficient is a scientific and practical tool to estimate the annual ET in the al- pine area. The annual ET in 2000 in the study area was 221.2 ram, 11.6 mm more than that in 1980. Average annual ET decreased from southeast to northwest, but the change of annual ET between 1980 and 2000 increased from southeast to northwest. As a vast and sparsely populated area, the population in the TRH region was extremely unbalanced and land use change was concentrated in very small regions. Thus, land use change had little effect on total annual ET in the study area but a great impact on its spatial distribution, and the effect of land use change on ET decreased with in- creasing precipitation. ET was most sensitive to the interconversion between forest and unused land, and was least sen- sitive to the interconversion between cropland and low-covered grassland.

Characteristics of root pullout resistance of Caragana korshinskii Kom.in the loess area of northeastern Qinghai-Tibet Plateau,China

Roots exert pullout resistance under pullout force,allowing plants to resist uprooting.However,the pullout resistance characteristics of taproot-type shrub species of different ages remain unclear.In this study,in order to improve our knowledge of pullout resistance characteristics of taproot systems of shrub species,we selected the shrub species Caragana korshinskii Kom.in different growth periods as the research plant and conducted in situ root pullout test.The relationships among the maximum pullout resistance,peak root displacement,shrub growth period,and aboveground growth indices(plant height and plant crown breadth)were analyzed,as well as the mechanical process of uprooting.Pullout resistance of 4-15 year-old C.korshinskii ranged from 2.49(±0.25)to 14.71(±4.96)kN,and the peak displacement ranged from 11.77(±8.61)to 26.50(±16.09)cm.The maximum pullout resistance and the peak displacement of roots increased as a power function(R^(2)=0.9038)and a linear function(R^(2)=0.8242)with increasing age,respectively.The maximum pullout resistance and the peak displacement increased with increasing plant height;however,this relationship was not significant.The maximum pullout resistance increased exponentially(R^(2)=0.5522)as the crown breadth increased.There was no significant relationship between the peak displacement and crown breadth.The pullout resistance and displacement curve were divided into three stages:the initial nonlinear growth,linear growth,and nonlinear stages.Two modes of failure of a single root occurred when the roots were subjected to vertical loading forces:the synchronous breakage mode and the periderm preferential breakage mode.These findings provide a foundation for further investigation of the soil reinforcement and slope protection mechanisms of this shrub species in the loess area of northeastern Qinghai-Tibet Plateau,China.

Cryostructures and ground ice content in ice-rich permafrost area of the Qinghai-Tibet Plateau with Computed Tomography Scanning

Permafrost is an important part of the cryosphere,playing an integral role in the hydrologic cycle,ecology,and influencing human activity.Melting of ground ice can drastically change landscapes and associated thaw subsidence may induce instability of infrastructure.The terrain conditions on the Qinghai-Tibet Plateau are complex,and the spatial distribution of ground ice is highly variable,so knowledge of its abundance and variability is required for impact assessments relating to the degradation of permafrost.This study examined 55 permafrost samples from warm,ice-rich permafrost region in Beiluhe Basin,Qinghai-Tibet Plateau.The samples were examined using Computed Tomography scanning,and the ice content and cryostructure were determined.The results indicated that:1)variation in volumetric ice content was considerable(0%-70%),with a mean value of 17%;2)seven cryostructures were identified,including crustal,vein,lenticular,ataxitic,reticulate and layered cryostructure;3)volumetric ice content varied by cryostructure,with the highest associated with layered and ataxitic cryostructures.Volumetric ice contents were lowest for samples with pore and lenticular cryostructures.This work provides detailed ground ice content and will be helpful for assessing thaw subsidence and infrastructure stability on Qinghai-Tibet Plateau.

Growth behavior and resource potential evaluation of gas hydrate in core fractures in Qilian Mountain permafrost area, Qinghai-Tibet Plateau

The Qilian Mountain permafrost area located in the northern of Qinghai-Tibet Plateau is a favorable place for natural gas hydrate formation and enrichment,due to its well-developed fractures and abundant gas sources.Understanding the formation and distribution of multi-component gas hydrates in fractures is crucial in accurately evaluating the hydrate reservoir resources in this area.The hydrate formation experiments were carried out using the core samples drilled from hydrate-bearing sediments in Qilian Mountain permafrost area and the multi-component gas with similar composition to natural gas hydrates in Qilian Mountain permafrost area.The formation and distribution characteristics of multi-component gas hydrates in core samples were observed in situ by X-ray Computed Tomography(X-CT)under high pressure and low temperature conditions.Results show that hydrates are mainly formed and distributed in the fractures with good connectivity.The ratios of volume of hydrates formed in fractures to the volume of fractures are about 96.8%and 60.67%in two different core samples.This indicates that the fracture surface may act as a favorable reaction site for hydrate formation in core samples.Based on the field geological data and the experimental results,it is preliminarily estimated that the inventory of methane stored in the fractured gas hydrate in Qilian Mountain permafrost area is about 8.67×1013 m3,with a resource abundance of 8.67×108 m3/km2.This study demonstrates the great resource potential of fractured gas hydrate and also provides a new way to further understand the prospect of natural gas hydrate and other oil and gas resources in Qilian Mountain permafrost area.

Mountain permafrost distribution modeling using Multivariate Adaptive Regression Spline (MARS) in the Wenquan area over the Qinghai-Tibet Plateau

In high mountainous areas, the development and distribution of alpine permafrost is greatly affected by macro- and mi- cro-topographic factors. The effects of latitude, altitude, slope, and aspect on the distribution of permafrost were studied to under- stand the dislribution patterns of permafrost in Wenquan on the Qinghai-Tibet Plateau. Cluster and correlation analysis were per- formed based on 30 m Global Digital Elevation Model （GDEM） data and field data obtained using geophysical exploration and borehole drilling methods. A Multivariate Adaptive Regression Spline model （MARS） was developed to simulate permafrost spa- tial distribution over the studied area. A validation was followed by comparing to 201 geophysical exploration sites, as well as by comparing to two other models, i.e., a binary logistic regression model and the Mean Annual Ground Temperature model （IVlAGT）. The MARS model provides a better simulation than the other two models. Besides the control effect of elevation on permafrost distribution, the MARS model also takes into account the impact of direct solar radiation on permafrost distribution.

Impacts of degrading permafrost on streamflow in the source area of Yellow River on the Qinghai-Tibet Plateau,China

Many observations in and model simulations for northern basins have confirmed an increased streamflow from degrading permafrost,while the streamflow has declined in the source area of the Yellow River(SAYR,above the Tanag hydrological station)on the northeastern Qinghai-Tibet Plateau,West China.How and to what extent does the degrading permafrost change the flow in the SAYR?According to seasonal regimes of hydrological processes,the SAYR is divided intofour sub-basins with varied permafrost extents to detect impacts of permafrost degradation on the Yellow River streamflow.Results show that permafrost degradation may have released appreciable meltwater for recharging groundwater.The potential release rate of ground-ice melt-water in the Sub-basin 1(the headwater area of the Yellow River(HAYR),above the Huangheyan hydrological station)is the highest(5.6 mm per year),contributing to 14.4%of the annual Yellow River streamflow at Huangheyan.Seasonal/intra-and annual shifts of streamflow,a possible signal for the marked alteration of hydrological processes by permafrost degradation,is observed in the HAYR,but the shifts are minor in other sub-basins in the SAYR.Improved hydraulic connectivity is expected to occur during and after certain degrees of permafrost degradation.Direct impacts of permafrost degradation on the annual Yellow River streamflow in the SAYR at Tanag,i.e.,from the meltwater of ground-ice,is estimated at 4.9%that of the annual Yellow River discharge at Tanag,yet with a high uncertainty,due to neglecting of the improved hydraulic connections from permafrost degradation and the flow generation conditions for the ground-ice meltwater.Enhanced evapotranspiration,substantial weakening of the Southwest China Autumn Rain,and anthropogenic disturbances may largely account for the declined streamflow in the SAYR.

Prevention and management of wind-blown sand damage along Qinghai-Tibet Railway in Cuonahu Lake area

This paper analyzes the characteristics of climate, geology and geomorphology, vegetation, and sand dune distribution in the Cuonahu Lake area beside the Qinghai-Tibet Railway. The types and causes of railway blown-sand hazards are discussed, and the effectiveness of various sand-controlling measures is assessed. From the perspective of integrated management, a sand-controlling system that combines several engineering measures, including nylon net sand barriers, concrete sand barriers, movable-board sand barriers, sand interception ditches, gravel/rock cover, film sandbags, and permanent vegetation is most beneficial.

Extracting water body data based on SDWI and threshold segmentation:A case study in permafrost area surrounding Salt Lake in Hoh Xil,Qinghai-Tibet Plateau,China

There are a large number of lakes,rivers,and other natural water bodies distributed in the permafrost area of the Qinghai-Tibet Plateau(QTP).The changes in water bodies will affect the distribution of water resources in sur-rounding areas and downstream areas,resulting in environmental impact and bringing potential flood disasters,which will induce more serious issues and problems in alpine and high-altitude areas with a fragile habitat(such as the QTP in China).Generally,effective,reasonable,and scientific monitoring of large-scale water bodies can not only document the changes in water bodies intuitively,but also provide important theoretical reference for subsequent environmental impact prediction,and disaster prevention and mitigation in due course of time.The large-scale water extraction technology derived from the optical remote sensing(RS)image is seriously affected by clouds,bringing about large differences among the extracted water result products.Synthetic aperture radar(SAR)RS technology has the unique advantage characteristics of all-weather,all-day,strong penetration,and not being affected by clouds,which is hopeful in extracting water body data,especially for days with cloudy weather.The data extraction of large-scale water bodies based on SAR images can effectively avoid the errors caused by clouds that become prevalent at present.In this paper,the Hoh Xil Salt Lake on the QTP and its surrounding five lakes are taken as the research objects.The 2-scene Sentinel-1 SAR image data covering the whole area on 22 August 2022 was used to verify the feasibility of extracting water body data in permafrost zones.Furthermore,on 22 August 2022,the wealth here was cloudy,which made the optical RS images,e.g.,Sentinel-2 images full of clouds.The results show that:using the Sentinel-1 image and threshold segmentation method to extract water body data is efficient and effective with excellent results in permafrost areas.Concretely,the Sentinel-1 dual-polarized water index(SDWI),calculated by combining dual vertical–vertical(VV)polarized and verti-cal–horizontal(VH)polarized data is a useful index for water extraction and the result is better than each of the VV or VH polarized images.

Large-scale characteristics of thermokarst lakes across the source area of the Yellow River on the Qinghai-Tibetan Plateau

As significant evidence of permafrost degradation,thermokarst lakes play an important role in the permafrost regions by regulating hydrology,ecology,and biogeochemistry.In the Sources Area of the Yellow River(SAYR),permafrost degradation has accelerated since the 1980s,and numerous thermokarst lakes have been discovered.In this paper,we use Sentinel-2 images to extract thermokarst lake boundaries and perform a regional-scale study on their geometry across the permafrost region in the SAYR.We also explored the spatiotemporal variations and potential drivers from the perspectives of the permafrost,climate,terrain and vegetation conditions.The results showed that there were 47,518 thermokarst lakes in 2021 with a total area of 190.22×106 m^(2),with an average size of 4,003.3 m^(2).The 44,928 ponds(≤10,000 m^(2))predominated the whole lake number(94.1%)but contributed to a small portion of the total lake area(28.8%).With 2,590 features(5.9%),small-sized(10,000 to 100,000 m^(2))and large-sized lakes(>100,000 m^(2))constituted up to 71.2%of the total lake area.Thermokarst lakes developed more significantly in warm permafrost regions than in cold permafrost areas;74.1%of lakes with a total area of 119.6×106 m^(2)(62.9%),were distributed in warm permafrost regions.Most thermokarst lakes were likely to develop within the elevation range of 4,500~4,800 m,on flat terrain(slope<10°),on SE and S aspects and in alpine meadow areas.The thermokarst lakes in the study region experienced significant shrinkage between 1990 and 2021,characterized by obvious lake drainage;the lake numbers decreased by 5418(56.1%),with a decreasing area of 58.63×106 m^(2)(49.0%).This shrinkage of the thermokarst lake area was attributable mainly to the intensified degradation of rich-ice permafrost thawing arising from continued climate warming,despite the wetting climatic trend.

Ecological-Based Mining:A Coal-Water-Thermal Collaborative Paradigm in Ecologically Fragile Areas in Western China

A substantial reduction in groundwater level,exacerbated by coal mining activities,is intensifying water scarcity in western China’s ecologically fragile coal mining areas.China’s national strategic goal of achieving a carbon peak and carbon neutrality has made eco-friendly mining that prioritizes the protection and efficient use of water resources essential.Based on the resource characteristics of mine water and heat hazards,an intensive coal-water-thermal collaborative co-mining paradigm for the duration of the mining process is proposed.An integrated system for the production,supply,and storage of mining companion resources is achieved through technologies such as roof water inrush prevention and control,hydrothermal quality improvement,and deep-injection geological storage.An active preventive and control system achieved by adjusting the mining technology and a passive system centered on multiobjective drainage and grouting treatment are suggested,in accordance with the original geological characteristics and dynamic process of water inrush.By implementing advanced multi-objective drainage,specifically designed to address the“skylight-type”water inrush mode in the Yulin mining area of Shaanxi Province,a substantial reduction of 50%in water drillings and inflow was achieved,leading to stabilized water conditions that effectively ensure subsequent safe coal mining.An integrated-energy complementary model that incorporates the clean production concept of heat utilization is also proposed.The findings indicate a potential saving of 8419 t of standard coal by using water and air heat as an alternative heating source for the Xiaojihan coalmine,resulting in an impressive energy conservation of 50.2%and a notable 24.2%reduction in carbon emissions.The ultra-deep sustained water injection of 100 m^(3)·h^(-1)in a single well would not rupture the formation or cause water leakage,and 7.87×10^(5)t of mine water could be effectively stored in the Liujiagou Formation,presenting a viable method for mine-water management in the Ordos Basin and providing insights for green and low-carbon mining.

Correlation of periodontal inflamed surface area with glycated hemoglobin,interleukin-6 and lipoprotein(a)in type 2 diabetes with retinopathy

BACKGROUND The two-way relationship between periodontitis and type 2 diabetes mellitus(T2DM)is well established.Prolonged hyperglycemia contributes to increased periodontal destruction and severe periodontitis,accentuating diabetic complications.An inflammatory link exists between diabetic retinopathy(DR)and periodontitis,but the studies regarding this association and the role of lipoprotein(a)[Lp(a)]and interleukin-6(IL-6)in these conditions are scarce in the literature.AIM To determine the correlation of periodontal inflamed surface area(PISA)with glycated Hb(HbA1c),serum IL-6 and Lp(a)in T2DM subjects with retinopathy.METHODS This cross-sectional study comprised 40 T2DM subjects with DR and 40 T2DM subjects without DR.All subjects were assessed for periodontal parameters[bleeding on probing(BOP),probing pocket depth,clinical attachment loss(CAL),oral hygiene index-simplified,plaque index(PI)and PISA],and systemic parameters[HbA1c,fasting plasma glucose and postprandial plasma glucose,fasting lipid profile,serum IL-6 and serum Lp(a)].RESULTS The proportion of periodontitis in T2DM with and without DR was 47.5%and 27.5%respectively.Severity of periodontitis,CAL,PISA,IL-6 and Lp(a)were higher in T2DM with DR group compared to T2DM without DR group.Significant difference was observed in the mean percentage of sites with BOP between T2DM with DR(69%)and T2DM without DR(41%),but there was no significant difference in PI(P>0.05).HbA1c was positively correlated with CAL(r=0.351,P=0.001),and PISA(r=0.393,P≤0.001)in study subjects.A positive correlation was found between PISA and IL-6(r=0.651,P<0.0001);PISA and Lp(a)(r=0.59,P<0.001);CAL and IL-6(r=0.527,P<0.0001)and CAL and Lp(a)(r=0.631,P<0.001)among study subjects.CONCLUSION Despite both groups having poor glycemic control and comparable plaque scores,the periodontal parameters were higher in DR as compared to T2DM without DR.Since a bidirectional link exists between periodontitis and DM,the presence of DR may have contributed to the severity of periodontal destruction and periodontitis may have influenced the progression of DR.

Research on the Relationship Between Environmental and Economic Coupling Systems in Bohai Bay Area Based on a Vector Autoregression(VAR)Model

This study analyzed the impact of land-based contaminants and tertiary industrial structure on economic development in the selected Bohai Bay area,China.Based on panel data spanning 2011-2020,a vector autoregressive(VAR)model is used to analyze and forecast the short-run and long-run relationships between three industrial structures,pollutant discharge,and economic development.The results showed that the environmental index had a long-term cointegration relationship with the industrial structure economic index.Per capital chemical oxygen demand(PCOD)and per capita ammonia nitrogen(PNH_(3)N)had a positive impact on delta per capita GDP(dPGDP),while per capita solid waste(PSW),the secondary industry rate(SIR)and delta tertiary industry(dTIR)had a negative impact on dPGDP.The VAR model under this coupling system had stability and credibility.The impulse response results showed that the short-term effect of the coupling system on dPGDP was basically consistent with the Granger causality test results.In addition,variance decomposition was used in this study to predict the long-term impact of the coupling system in the next ten periods(i.e.,ten years).It was found that dTIR had a great impact on dPGDP,with a contribution rate as high as 74.35%in the tenth period,followed by the contribution rate of PCOD up to 3.94%,while the long-term contribution rates of PSW,SIR and PNH3N were all less than 1%.The results show that the government should support the development of the tertiary industry to maintain the vitality of economic development and prevent environmental deterioration.

Seismic response of a mid-story isolated structure considering SSI in mountainous areas under long-period earthquakes

At present,there is not much research on mid-story isolated structures in mountainous areas.In this study,a model of a mid-story isolated structure considering soil-structure interaction(SSI)in mountainous areas is established along with a model that does not consider SSI.Eight long-period earthquake waves and two ordinary earthquake waves are selected as inputs for the dynamic time history analysis of the structure.The results show that the seismic response of a mid-story isolated structure considering SSI in mountainous areas can be amplified when compared with a structure that does not consider SSI.The structure response under long-period earthquakes is larger than that of ordinary earthquakes.The structure response under far-field harmonic-like earthquakes is larger than that of near-fault pulse-type earthquakes.The structure response under near-fault pulse-type earthquakes is larger than that of far-field non-harmonic earthquakes.When subjected to long-period earthquakes,the displacement of the isolated bearings exceeded the limit value,which led to instability and overturning of the structure.The structure with dampers in the isolated story could adequately control the nonlinear response of the structure,effectively reduce the displacement of the isolated bearings,and provide a convenient,efficient and economic method not only for new construction but also to retrofit existing structures.

Biological soil crusts and their potential applications in the sand land over Qinghai-Tibet Plateau

The Qinghai-Tibet Plateau is now experiencing ecological degradation risks as a result of climate change and human activities.The alpine grassland ecology in permafrost zones is fragile and susceptible to deterioration due to its high altitude,low temperature,and limited oxygen,which complicates the repair of damaged land.Biological soil crusts(BSCs)are crucial for land restoration in plateau regions because they can thrive in harsh conditions and have environmentally beneficial traits.Inoculated biological soil crust(IBSC)has shown success in low-altitude desert regions,but may not be easily duplicated to the plateau environment.Therefore,it is essential to do a comprehensive and multifaceted analysis of the basic theoretical comprehension and practical application of BSCs on the Tibetan Plateau.This review article aims to provide a brief summary of the ecological significance and the mechanisms related to the creation,growth,and progression of BSCs.It discusses the techniques used for cultivating BSCs in laboratories and using them in the field,focusing on the Qinghai-Tibet Plateau circumstance.We thoroughly discussed the potential and the required paths for further studies.This study may be used as a basis for selecting suitable microbial strains and accompanying supplemental actions for implementing IBSCs in the Qinghai-Tibet Plateau.

Significant lake expansion has accelerated permafrost degradation on the Qinghai-Tibet Plateau

In recent years, lakes on the Qinghai-Tibet Plateau have become more responsive to climate change. In September 2011, Zonag Lake in Hoh Xil experienced sudden drainage, the water eventually flowed into Yanhu Lake, which caused Yanhu Lake to continue to expand. The potential collapse of Yanhu Lake could directly threaten the operational safety of the adjacent Qinghai-Tibet Highway, Qinghai-Tibet Railway. To explore the implications of expanding lakes on the surrounding permafrost, we selected Hoh Xil Yanhu Lake on the Qinghai-Tibet Plateau to study the effect of lake expansion on permafrost degradation. The permafrost degradation in the Yanhu Lake basin from October 2017 to December 2022 was inverted using Sentinel-1 satellite image data and small baseline subset interferometry synthetic aperture radar(SBAS-In SAR) technology. Additionally, permafrost degradation from February 2007 and February 2010 was analyzed using advanced land observing satellite phased array-type L-band synthetic aperture radar(ALOS PALSAR) satellite images and differential interferometric synthetic aperture radar(D-In SAR) technique. The results showed that the permafrost around Yanhu Lake experienced accelerated degradation. Prior to the expansion of Yanhu Lake, the average annual deformation rate along the line of sight(LOS) direction was 6.7 mm/yr. After the expansion, the rate increased to 20.9 mm/yr. The integration of spatial-temporal distribution maps of surface subsidence, Wudaoliang borehole geothermal data, meteorological data, Yanhu Lake surface area changes, and water level changes supports the assertion that the intensified permafrost degradation could be attributed to lake expansion rather than the rising air temperature. Furthermore, permafrost degradation around Yanhu Lake could impact vital infrastructure such as the adjacent Qinghai-Tibet Highway and Qinghai-Tibet Railway.

Central Asia revealed as a key area in evolution of Eremurus (Asphodelaceae)

Eremurus was described at the beginning of the 19th century.However,due to limited sampling and the small number of gene markers to date,its phylogeny and evolution are largely unknown.In this study,we analyzed plastomes from 27 species belonging to 2 subgenera and 3 sections of Eremurus,which are found in Central Asia(its center of diversity)and China.We also analyzed nuclear DNA ITS of 33 species,encompassing all subgenera and sections of the genus in Central Asia,southwest Asia and China.Our findings revealed that the genus was monophyletic,although both subgenera Eremurus and Henningia were found to be paraphyletic.Both plastome and nrDNA-based phylogenetic trees had three clades that did not reflect the current taxonomy of the genus.Our biogeographical and time-calibrated trees suggest that Eremurus originated in the ancient Tethyan area in the second half of the Eocene.Diversification of Eremurus occurred from the early Oligocene to the late Miocene.Paratethys Sea retreat and several orogenetic events,such as the progressive uplift of the Qinghai-Tibet Plateau and surrounding mountain belts(Altai,Pamir,Tian Shan),caused serious topographic and climate(aridification)changes in Central Asia that may have triggered a split of clades and speciation.In this transformed Central Asia,speciation proceeded rapidly driven mainly by vicariance caused by numerous mountain chains and specialization to a variety of climatic,topographic and soil conditions that exist in this region.

Cryptic diversity and rampant hybridization in annual gentians on the Qinghai-Tibet Plateau revealed by population genomic analysis

Understanding the evolutionary and ecological processes involved in population differentiation and speciation provides critical insights into biodiversity formation. In this study, we employed 29,865 single nucleotide polymorphisms(SNPs) and complete plastomes to examine genomic divergence and hybridization in Gentiana aristata, which is endemic to the Qinghai-Tibet Plateau(QTP) region. Genetic clustering revealed that G. aristata is characterized by geographic genetic structures with five clusters(West, East, Central, South and North). The West cluster has a specific morphological character(i.e., blue corolla) and higher values of FSTcompared to the remaining clusters, likely the result of the geological barrier formed by the Yangtze River. The West cluster diverged from the other clusters in the Early Pliocene;these remaining clusters diverged from one another in the Early Quaternary. Phylogenetic reconstructions based on SNPs and plastid data revealed substantial cyto-nuclear conflicts. Genetic clustering and D-statistics demonstrated rampant hybridization between the Central and North clusters,along the Bayankala Mountains, which form the geological barrier between the Central and North clusters. Species distribution modeling demonstrated the range of G. aristata expanded since the Last Interglacial period. Our findings provide genetic and morphological evidence of cryptic diversity in G. aristata, and identified rampant hybridization between genetic clusters along a geological barrier.These findings suggest that geological barriers and climatic fluctuations have an important role in triggering diversification as well as hybridization, indicating that cryptic diversity and hybridization are essential factors in biodiversity formation within the QTP region.

Diversity of Orchids in Terms of Their Distribution, Uses and Conservation in Annapurna Conservation Area of Nepal

The Annapurna Conservation Area (ACA), the first conservation area and the largest protected area (PA) in Nepal, is incredibly rich in biodiversity. Notwithstanding this, orchids in the ACA have not been explored enough yet thus making the need for ambitious research to be carried out. Previous study only included 81 species of orchids within ACA. This study aims to update the record of species and genera richness in the ACA. In total 198 species of orchids, belonging to 67 genera (40% and 62% of the total recorded orchid species and genera in Nepal) has been recorded in ACA. This represents an increase of 144% in species and 56% in genera over the previous data. Out of the 198 species, 99 were epiphytes, 6 were holomycotrophic and 93 were terrestrial. Among the 67 genera, Bulbophyllum (17) species were dominant, followed by Dendrobium (16), Herminium (10), Coelogyne, Plantanthera (9 each), Eria, Habenaria, Oberonia (8 each), Calanthe (7), and Liparis (6). Fifty-six species were found to be ornamentally significant and 85 species medicinally significant.

Unveiling evapotranspiration patterns and energy balance in a subalpine forest of the Qinghai-Tibet Plateau:observations and analysis from an eddy covariance system

Evapotranspiration is an important parameter used to characterize the water cycle of ecosystems.To under-stand the properties of the evapotranspiration and energy balance of a subalpine forest in the southeastern Qinghai-Tibet Plateau,an open-path eddy covariance system was set up to monitor the forest from November 2020 to October 2021 in a core area of the Three Parallel Rivers in the Qing-hai-Tibet Plateau.The results show that the evapotranspira-tion peaked daily,the maximum occurring between 11:00 and 15:00.Environmental factors had significant effects on evapotranspiration,among them,net radiation the greatest(R^(2)=0.487),and relative humidity the least(R^(2)=0.001).The energy flux varied considerably in different seasons and sensible heat flux accounted for the main part of turbulent energy.The energy balance ratio in the dormant season was less than that in the growing season,and there is an energy imbalance at the site on an annual time scale.

The environmental analysis and site selection of mussel and large yellow croaker aquaculture areas based on high resolution remote sensing

Mussel aquaculture and large yellow croaker aquaculture areas and their environmental characteristics in Zhoushan were analyzed using satellite data and in-situ surveys.A new two-step remote sensing method was proposed and applied to determine the basic environmental characteristics of the best mussel and large yellow croaker aquaculture areas.This methodology includes the first step of extraction of the location distribution and the second step of the extraction of internal environmental factors.The fishery ranching index(FRI1,FRI2)was established to extract the mussel and the large yellow croaker aquaculture area in Zhoushan,using Gaofen-1(GF-1)and Gaofen-6(GF-6)satellite data with a special resolution of 2 m.In the second step,the environmental factors such as sea surface temperature(SST),chlorophyll a(Chl-a)concentration,current and tide,suspended sediment concentration(SSC)in mussel aquaculture area and large yellow croaker aquaculture area were extracted and analyzed in detail.The results show the following three points.(1)For the extraction of the mussel aquaculture area,FRI1 and FRI2 are complementary,and the combination of FRI1 and FRI2 is suitable to extract the mussel aquaculture area.As for the large yellow croaker aquaculture area extraction,FRI2 is suitable.(2)Mussel aquaculture and the large yellow croaker aquaculture area in Zhoushan are mainly located on the side near the islands that are away from the eastern open waters.The water environment factor template suitable for mussel and large yellow croaker aquaculture was determined.(3)This two-step remote sensing method can be used for the preliminary screening of potential site selection for the mussels and large yellow croaker aquaculture area in the future.the fishery ranching index(FRI1,FRI2)in this paper can be applied to extract the mussel and large yellow croaker aquaculture areas in coastal waters around the world.