Objective:To study the effects of Traditional Chinese Medicine Jianpi Chushi decoction and ointment on chronic eczema.Methods:DNCB acetone solution was used to sensitize the skin of back and ears of 36 rats in order t...Objective:To study the effects of Traditional Chinese Medicine Jianpi Chushi decoction and ointment on chronic eczema.Methods:DNCB acetone solution was used to sensitize the skin of back and ears of 36 rats in order to establish chronic eczema model.A total of 36 rats were divided into four groups of 9 randomly including oral medicine group,external inunctum group,combination therapy group,and model control group respectively.Besides,the blank group of 4 healthy rats were set.The oral medicine group was given Traditional Chinese Medicine Jianpi Chushi decoction [(Poria cocos,Chinese yam,Cortex dictamni,Zaocys dhumnade,Rhizoma atractylodis,Pericarpium citri reticulatae,Scutellaria baicalensis,Radix Sophorae Flavescentis,Raw Radix Paeoniae Alba,Licorice roots(Northwest Origin)] by gastric infusion(1.6 g/m L·5 m L/d); the external inunctum group was given Qingpeng ointment on the skin,the combination therapy group was given Jianpi Chushi decoction by gastric infusion and Qingpeng ointment combination therapy.The model control group was given normal saline(NS) of the same volume by gastric infusion and vaseline on skin.Continuous administration 15 d and stopped for 3 d.The thickness difference and weight difference of left and right ear of every group were measured and the degree of ear swelling were evaluated.The CD4^+ and CD8^+ content and the IL-2,IL-4 level of serum were detected,and the inflammatory cells counts of back skin were recorded.Results:After treatment,the degree of ear swelling of oral medicine group,external inunctum group and combination therapy group significant decreased compared with model control group(P<0.05).The CD4^+,CD8^+ cell content and IL-2 level of oral medicine group,external inunctum group,combination therapy group and model control group significant decreased compared with blank group,and IL-2 level and the inflammatory cells count increased.After 15 d of treatment,the CD4^+,CD8^+ cell content and IL-2 level of serum of oral medicine group,external inunctum group and combination therapy group raised and the IL-4 level and the inflammatory cells count had significant decreased compared with model control group,and the effect of combination therapy group was more obvious(P<0.05).Conclusions:Qral Jianpi Chushi decoction could treat chronic eczema effectively,and oral Chinese medicine combined with ointment could enhance and speed up the efficacy.展开更多
目的分析青鹏软膏对湿疹皮炎大鼠治疗的作用机理。方法将湿疹皮炎造模成功的14只大鼠随机分成对照组和研究组,对照组不给予任何处理,研究组给予青鹏软膏治疗,另选取7只大鼠为正常组,采取免疫组织化学法检测皮肤组织中高迁移率族蛋白1(hi...目的分析青鹏软膏对湿疹皮炎大鼠治疗的作用机理。方法将湿疹皮炎造模成功的14只大鼠随机分成对照组和研究组,对照组不给予任何处理,研究组给予青鹏软膏治疗,另选取7只大鼠为正常组,采取免疫组织化学法检测皮肤组织中高迁移率族蛋白1(high mobility group protein,HMGB)、Toll样受体4(Toll like receptor 4,TLR4)及白细胞介素(interleukin-17,IL-17)蛋白表达水平。结果对照组BUN显著高于正常组,研究组BUN显著低于对照组,而高于正常组(P<0.05)。研究组和对照组大鼠IL-17、HMGB1、TLR4在皮肤组织中的表达水平均较正常组明显升高,而研究组大鼠3项指标水平较对照组大鼠明显下降,上述对比差异均有统计学意义(P<0.05)。结论青鹏软膏抑制湿疹皮炎大鼠HMGB1、TLR4、IL-17表达,并改善症状。展开更多
文摘Objective:To study the effects of Traditional Chinese Medicine Jianpi Chushi decoction and ointment on chronic eczema.Methods:DNCB acetone solution was used to sensitize the skin of back and ears of 36 rats in order to establish chronic eczema model.A total of 36 rats were divided into four groups of 9 randomly including oral medicine group,external inunctum group,combination therapy group,and model control group respectively.Besides,the blank group of 4 healthy rats were set.The oral medicine group was given Traditional Chinese Medicine Jianpi Chushi decoction [(Poria cocos,Chinese yam,Cortex dictamni,Zaocys dhumnade,Rhizoma atractylodis,Pericarpium citri reticulatae,Scutellaria baicalensis,Radix Sophorae Flavescentis,Raw Radix Paeoniae Alba,Licorice roots(Northwest Origin)] by gastric infusion(1.6 g/m L·5 m L/d); the external inunctum group was given Qingpeng ointment on the skin,the combination therapy group was given Jianpi Chushi decoction by gastric infusion and Qingpeng ointment combination therapy.The model control group was given normal saline(NS) of the same volume by gastric infusion and vaseline on skin.Continuous administration 15 d and stopped for 3 d.The thickness difference and weight difference of left and right ear of every group were measured and the degree of ear swelling were evaluated.The CD4^+ and CD8^+ content and the IL-2,IL-4 level of serum were detected,and the inflammatory cells counts of back skin were recorded.Results:After treatment,the degree of ear swelling of oral medicine group,external inunctum group and combination therapy group significant decreased compared with model control group(P<0.05).The CD4^+,CD8^+ cell content and IL-2 level of oral medicine group,external inunctum group,combination therapy group and model control group significant decreased compared with blank group,and IL-2 level and the inflammatory cells count increased.After 15 d of treatment,the CD4^+,CD8^+ cell content and IL-2 level of serum of oral medicine group,external inunctum group and combination therapy group raised and the IL-4 level and the inflammatory cells count had significant decreased compared with model control group,and the effect of combination therapy group was more obvious(P<0.05).Conclusions:Qral Jianpi Chushi decoction could treat chronic eczema effectively,and oral Chinese medicine combined with ointment could enhance and speed up the efficacy.
文摘目的分析青鹏软膏对湿疹皮炎大鼠治疗的作用机理。方法将湿疹皮炎造模成功的14只大鼠随机分成对照组和研究组,对照组不给予任何处理,研究组给予青鹏软膏治疗,另选取7只大鼠为正常组,采取免疫组织化学法检测皮肤组织中高迁移率族蛋白1(high mobility group protein,HMGB)、Toll样受体4(Toll like receptor 4,TLR4)及白细胞介素(interleukin-17,IL-17)蛋白表达水平。结果对照组BUN显著高于正常组,研究组BUN显著低于对照组,而高于正常组(P<0.05)。研究组和对照组大鼠IL-17、HMGB1、TLR4在皮肤组织中的表达水平均较正常组明显升高,而研究组大鼠3项指标水平较对照组大鼠明显下降,上述对比差异均有统计学意义(P<0.05)。结论青鹏软膏抑制湿疹皮炎大鼠HMGB1、TLR4、IL-17表达,并改善症状。
