Effect of acupuncture on cerebral hematoma volume and HO-1 expression in rats with acute cerebral hemorrhage

Objective:To explore the effect of acupuncture on the expression of heme oxygenase-1 in rats with acute cerebral hemorrhage.Methods:108 Wistar male rats were randomly divided into sham operation group,model group,and acupuncture combined with model group(referred to as acupuncture group).Each group was divided into 3 subgroups according to 1d,3d and 7d,with 6 rats in each subgroup.The rat model of cerebral hemorrhage was established by autologous blood injection.Acupuncture was given at Baihui(GU20)and Qubin(GB7).Separately,at the 1st,3rd and 7th day,modified neurological severity score was used to evaluate the neurological function of rats,HE staining was used to measure the volume of cerebral hematoma and western blot was used to detect the expression of heme oxygenase-1 protein in cerebral hematoma tissue.Results:Compared with the model group,at each time point,the modified neurological severity score of the acupuncture group was significantly reduced(P<0.01);at the two time points of 3rd and 7th day,the cerebral hematoma volume of the acupuncture group was significantly reduced(P<0.05)and the expression of heme oxygenase-1 protein in brain tissue was significantly increased(P<0.05).Conclusion:Acupuncture may promote the expression of heme oxygenase-1 protein,reduce the volume of hematoma and the score of neurological deficit in rats with intracerebral hemorrhage,improve the performance of neurological deficit,and play a role in brain protection.

百会透曲鬓穴为主治疗小儿脑瘫疗效观察

目的:观察百会透曲鬓穴为主治疗小儿脑瘫的临床疗效。方法:60例脑瘫患儿随机分为对照组30例和治疗组30例。对照组给予肌注神经节苷脂;治疗组在此基础上给予百会透曲鬓穴为主、体穴点刺为辅的方法进行针刺治疗。治疗3个疗程后,分别进行粗大运动功能量表(GMFM)评估,对比两组的疗效。结果:治疗组GMFM评分高于对照组,差异有统计学意义(P<0.05)。结论:百会透曲鬓穴为主治疗小儿脑瘫疗效肯定,在常规药物治疗基础上介入此针刺法能明显地提高疗效。