Quorum systems have been used to solve the problem of data consistency in distributed fault-tolerance systems. But when intrusions occur, traditional quorum systems have some disadvantages. For example, synchronous qu...Quorum systems have been used to solve the problem of data consistency in distributed fault-tolerance systems. But when intrusions occur, traditional quorum systems have some disadvantages. For example, synchronous quorum systems are subject to DOS attacks, while asynchronous quorum systems need a larger system size （at least 3f＋1 for generic data, and f fewer for self-verifying data）. In order to solve the problems above, an intrusion-tolerance quorum system （ITQS） of hybrid time model based on trust timely computing base is presented （TTCB）. The TTCB is a trust secure real-time component inside the server with a well defined interface and separated from the operation system. It is in the synchronous communication environment while the application layer in the server deals with read-write requests and executes update-copy protocols asynchronously. The architectural hybridization of synchrony and asynchrony can achieve the data consistency and availability correctly. We also build two kinds of ITQSes based on TTCB, i.e., the symmetrical and the asymmetrical TTCB quorum systems. In the performance evaluations, we show that TTCB quorum systems are of smaller size, lower load and higher availability.展开更多
Rapid developments in network systems of business service have resulted in more reliance on distributed computing, typified by “subscriber/push” architectures. Unfortunately, frequent and unexpectable network failur...Rapid developments in network systems of business service have resulted in more reliance on distributed computing, typified by “subscriber/push” architectures. Unfortunately, frequent and unexpectable network failures were routine, and downtime was not in hours, but in days. High availability has become the most important factor decreasing business risk and improving Quality of Service. Cluster technology has solved the non-stop problem on Local Area Network. However, most technologies including cluster today fail to ensure the non-stop Internet service based on Routers. With good performance on high availability and fault tolerance, quorum systems are very suitable for application to distributed business service networks. In this work, we modeled and developed a non-stop Internet service system based on a new quorum system, circle quorum system, for Boston Mutual Fund Broker, US. With five protocols, it provided highly available data services for clients on Internet.展开更多
Quorum system is a preferable model to construct distributed access control architecture, but not all quorum system can satisfy the requirements of distributed access control architecture. Aiming at the dependable pro...Quorum system is a preferable model to construct distributed access control architecture, but not all quorum system can satisfy the requirements of distributed access control architecture. Aiming at the dependable problem of authorization server in distributed system and combining the requirements of access control, a set of criterions to select and evaluate quorum system is presented. The scheme and algorithm of constructing an authorization server system based on Paths quorum system are designed, and the integrated sys- tem performance under some servers attacked is fully analyzed. Role-based access control on the Web implemented by this scheme is introduced. Analysis shows that with certain node failure probability, the scheme not only has high dependability but also can satisfy the special requirements of distributed access control such as real-time, parallelism, and consistency of security policy.展开更多
Quorum sensing （QS） is a bacterial cell-cell communication process by which bacteria communicate using extracellular signals called autoinducers. Two QS systems have been identified in Escherichia coli K-12, includi...Quorum sensing （QS） is a bacterial cell-cell communication process by which bacteria communicate using extracellular signals called autoinducers. Two QS systems have been identified in Escherichia coli K-12, including an intact QS system 2 that is stimulated by the cyclic AMP （cAMP）-cAMP receptor protein （CRP） complex and a partial QS system 1 that consists of SdiA （suppressor of cell division inhibitor） responding to signals generated by other microbial species. The relationship between QS system 1 and system 2 in E. coli, however, remains obscure. Here, we show that an EAL domain protein, encoded by ydiV, and cAMP are involved in the interaction between the two QS systems in E. coli. Expression of sdiA and ydiV is inhibited by glucose. SdiA binds to the ydiV promoter region in a dose-dependent, but nonspecific, manner; extracellular autoinducer 1 from other species stimulates ydiV expression in an sdiA-depen- dent manner. Furthermore, we discovered that the double sdiA-ydiV mutation, but not the single mutation, causes a 2-fold decrease in intracellular cAMP concentration that leads to the inhibition of QS system 2. These results indicate that signaling pathways that respond to important environmental cues, such as autoinducers and glucose, are linked together for their control in E. coli.展开更多
Quorum sensing(QS)system can dynamically control the expression of proteins along with the cell growth.The promoting period of QS system has been little focused on until now.In this study,a self-induced dynamic regula...Quorum sensing(QS)system can dynamically control the expression of proteins along with the cell growth.The promoting period of QS system has been little focused on until now.In this study,a self-induced dynamic regulated expression(SIDRE)system was constructed in Escherichia coli.To enable the system suitable for the expression of enzymes,promoter engineering was used to obtain P_(luxI)mutants.To test the SIDRE system,alginate lyase AL493 and esterase Est7 were used as target protein for expression.The enzyme activity of alginate lyase and esterase reached 96.38%and 106.71%of the control strains containing the T7 promoter.In high-density fermentation,the activity of alginate lyase expressed by the SIDRE system with P_(luxI)(T-38C)as promoter was 4.34-fold of that expressed by the T7 promoter.Therefore,the P_(luxI)mutants with different promoting periods and/or different strengths show great potential in both laboratory and industrial scale for protein expression.展开更多
Objective: This study focused on the antibacterial and anti-proliferative activity of extracts from Carica papaya and Cocos nucifera roots. Methodology: The minimum inhibitory concentration and the minimum bactericida...Objective: This study focused on the antibacterial and anti-proliferative activity of extracts from Carica papaya and Cocos nucifera roots. Methodology: The minimum inhibitory concentration and the minimum bactericidal concentration of the extracts on Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutans, and Staphylococcus aureus were deduced by the microdilution method. The anti-biofilm activity was determined on all four strains and anti-quorum sensing activity by inhibition of violacein production in Chromobacterium violaceum. Anti-proliferative activity on prostate cultured cancer cells was evaluated by MTT assay. Sterols and triterpenes were also assayed in this study. Results: The methanolic extract of Carica papaya showed the best anti-biofilm effect with a percentage inhibition of 66.10 ± 1.79. The methanolic extract of Cocos nucifera had the strongest inhibition on the production of quorum sensing (61.42 ± 0.28). In addition, the methanolic extract of Cocos nucifera roots showed the best cytotoxic effect on prostate cancer LNCaP cell lines (IC<sub>50</sub> = 26.98 ± 2.6 μg/mL) and Carica papaya on the PC-3 lines (IC<sub>50</sub> = 127.20 ± 5.99 μg/mL). The extracts were also rich in triterpenes and sterols. Conclusion: This study provides support for the ethnomedical use of Carica papaya and Cocos nucifera roots as an antimicrobial and anticancer.展开更多
Bacterial cells rely on signaling molecules to communicate with others from the same species and induce certain genes in a process known as quorum sensing (QS). A common molecule is N-acyl homoserine lactone (AHL) whi...Bacterial cells rely on signaling molecules to communicate with others from the same species and induce certain genes in a process known as quorum sensing (QS). A common molecule is N-acyl homoserine lactone (AHL) which is responsible for the expression of virulence and other factors that allow the organisms to compete in a given environment. On the other hand, other bacteria produce certain enzymes such as AHL-lactonase that break down AHL molecules and prevent gene expression of these factors. The aim of this work was to examine the level of degradation of AHL molecules by AHL-lactonase in 62 Bacillus thuringiensis (Bt) strains isolated from Middle Tennessee, Mississippi, and Alabama. N-hexanoyl-homoserine lactone (C<sub>6</sub>-HSL) and N-3-oxo-hexanoyl homoserine lactone (3-oxo-C<sub>6</sub>-HSL), which cause Chromobacterium violaceum (CV026) to produce a purple pigment were tested at different concentrations to view the Bt lactonase activity. In addition, PCR was used to test for the presence of the lactonase gene. The results showed that among the 62 Bt strains, there were 58 that possessed the AHL-lactonase (aiiA) gene and 48 strains were able to degrade C<sub>6</sub>-HSL. At high concentrations of AHL, only 13 strains were able to completely degrade C6-HSL. In addition, degradation of 3-oxo-C<sub>6</sub>-HSL was weak compared to C<sub>6</sub>-HSL. The results also revealed that AHL lactonase was thermostable, and it was concluded that the level of degradation varies in Bt strains. Only 13 of the strains studied have potent inhibitory activity against C<sub>6</sub>-HSL, which may be good to be used in field applications to control agricultural pest.展开更多
在无线传感器网络中,异步低占空比技术可以极大地降低能耗,但是由于节点的低占空比唤醒会造成极大的端到端数据时延。针对这个问题提出一种基于Quorum的异步自适应低占空比路由算法ORDA(Optimal-Reliable delay routing algorithm for l...在无线传感器网络中,异步低占空比技术可以极大地降低能耗,但是由于节点的低占空比唤醒会造成极大的端到端数据时延。针对这个问题提出一种基于Quorum的异步自适应低占空比路由算法ORDA(Optimal-Reliable delay routing algorithm for low duty cycle WSNs based on Quorum),将异步占空比网络和实际链路模型相结合,在异步占空比网络中节点在不同时刻的邻居发现延迟也在不断变化。首先为每个节点根据网络负载选择自身的Quorum类型,并利用Quorum特性来计算邻居节点的重叠时隙个数;然后根据链路质量进一步计算出这一跳范围内邻居节点间的成功转发预期值,并在即将唤醒的节点中选择更可靠的节点转发数据。仿真实验证明,该算法不仅能够降低端到端延迟,而且能获得很好的转发成功率。展开更多
针对无线传感器网络占空比MAC协议在链路或节点失效环境下易导致严重的能量浪费问题,提出了一种基于Grid Quorum的异步低占空比M AC协议-AGQ-M AC(Asynchronous Grid Quorum M AC).AGQ-M AC采用Grid Quorum组分配信道,通过动态调配Quoru...针对无线传感器网络占空比MAC协议在链路或节点失效环境下易导致严重的能量浪费问题,提出了一种基于Grid Quorum的异步低占空比M AC协议-AGQ-M AC(Asynchronous Grid Quorum M AC).AGQ-M AC采用Grid Quorum组分配信道,通过动态调配Quorum比率得到了节点的最优占空比,并在邻节点发现过程中采用双前导序文抽样来监测信道状态,以减少节点唤醒时间从而达到能量高效.仿真实验结果表明,与其他占空比MAC协议相比,AGQ-MAC延长了网络生存时间,降低了网络能耗,提高了邻发现数据传输率,同时保持较低的平均邻发现延迟.展开更多
基金supported by the National Natural Science Foundation of China (60774091)
文摘Quorum systems have been used to solve the problem of data consistency in distributed fault-tolerance systems. But when intrusions occur, traditional quorum systems have some disadvantages. For example, synchronous quorum systems are subject to DOS attacks, while asynchronous quorum systems need a larger system size （at least 3f＋1 for generic data, and f fewer for self-verifying data）. In order to solve the problems above, an intrusion-tolerance quorum system （ITQS） of hybrid time model based on trust timely computing base is presented （TTCB）. The TTCB is a trust secure real-time component inside the server with a well defined interface and separated from the operation system. It is in the synchronous communication environment while the application layer in the server deals with read-write requests and executes update-copy protocols asynchronously. The architectural hybridization of synchrony and asynchrony can achieve the data consistency and availability correctly. We also build two kinds of ITQSes based on TTCB, i.e., the symmetrical and the asymmetrical TTCB quorum systems. In the performance evaluations, we show that TTCB quorum systems are of smaller size, lower load and higher availability.
文摘Rapid developments in network systems of business service have resulted in more reliance on distributed computing, typified by “subscriber/push” architectures. Unfortunately, frequent and unexpectable network failures were routine, and downtime was not in hours, but in days. High availability has become the most important factor decreasing business risk and improving Quality of Service. Cluster technology has solved the non-stop problem on Local Area Network. However, most technologies including cluster today fail to ensure the non-stop Internet service based on Routers. With good performance on high availability and fault tolerance, quorum systems are very suitable for application to distributed business service networks. In this work, we modeled and developed a non-stop Internet service system based on a new quorum system, circle quorum system, for Boston Mutual Fund Broker, US. With five protocols, it provided highly available data services for clients on Internet.
基金Supported by the National Natural Science Foundation of China (70771043, 60873225, 60773191)
文摘Quorum system is a preferable model to construct distributed access control architecture, but not all quorum system can satisfy the requirements of distributed access control architecture. Aiming at the dependable problem of authorization server in distributed system and combining the requirements of access control, a set of criterions to select and evaluate quorum system is presented. The scheme and algorithm of constructing an authorization server system based on Paths quorum system are designed, and the integrated sys- tem performance under some servers attacked is fully analyzed. Role-based access control on the Web implemented by this scheme is introduced. Analysis shows that with certain node failure probability, the scheme not only has high dependability but also can satisfy the special requirements of distributed access control such as real-time, parallelism, and consistency of security policy.
文摘Quorum sensing （QS） is a bacterial cell-cell communication process by which bacteria communicate using extracellular signals called autoinducers. Two QS systems have been identified in Escherichia coli K-12, including an intact QS system 2 that is stimulated by the cyclic AMP （cAMP）-cAMP receptor protein （CRP） complex and a partial QS system 1 that consists of SdiA （suppressor of cell division inhibitor） responding to signals generated by other microbial species. The relationship between QS system 1 and system 2 in E. coli, however, remains obscure. Here, we show that an EAL domain protein, encoded by ydiV, and cAMP are involved in the interaction between the two QS systems in E. coli. Expression of sdiA and ydiV is inhibited by glucose. SdiA binds to the ydiV promoter region in a dose-dependent, but nonspecific, manner; extracellular autoinducer 1 from other species stimulates ydiV expression in an sdiA-depen- dent manner. Furthermore, we discovered that the double sdiA-ydiV mutation, but not the single mutation, causes a 2-fold decrease in intracellular cAMP concentration that leads to the inhibition of QS system 2. These results indicate that signaling pathways that respond to important environmental cues, such as autoinducers and glucose, are linked together for their control in E. coli.
基金supported by the National Natural Science Foundation of China(31922072)Natural Science Foundation of Shandong Province(ZR2020JQ15)+3 种基金Project of Shandong Province Higher Educational Science and Technology Program(2019KJF012)Taishan Scholar Project of Shandong Province(tsqn201812020)China Agriculture Research System(CARS-48)Qingdao Science and Technology Demonstration and Guidance Project for Benefiting the People(20-3-4-28-nsh)。
文摘Quorum sensing(QS)system can dynamically control the expression of proteins along with the cell growth.The promoting period of QS system has been little focused on until now.In this study,a self-induced dynamic regulated expression(SIDRE)system was constructed in Escherichia coli.To enable the system suitable for the expression of enzymes,promoter engineering was used to obtain P_(luxI)mutants.To test the SIDRE system,alginate lyase AL493 and esterase Est7 were used as target protein for expression.The enzyme activity of alginate lyase and esterase reached 96.38%and 106.71%of the control strains containing the T7 promoter.In high-density fermentation,the activity of alginate lyase expressed by the SIDRE system with P_(luxI)(T-38C)as promoter was 4.34-fold of that expressed by the T7 promoter.Therefore,the P_(luxI)mutants with different promoting periods and/or different strengths show great potential in both laboratory and industrial scale for protein expression.
文摘Objective: This study focused on the antibacterial and anti-proliferative activity of extracts from Carica papaya and Cocos nucifera roots. Methodology: The minimum inhibitory concentration and the minimum bactericidal concentration of the extracts on Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutans, and Staphylococcus aureus were deduced by the microdilution method. The anti-biofilm activity was determined on all four strains and anti-quorum sensing activity by inhibition of violacein production in Chromobacterium violaceum. Anti-proliferative activity on prostate cultured cancer cells was evaluated by MTT assay. Sterols and triterpenes were also assayed in this study. Results: The methanolic extract of Carica papaya showed the best anti-biofilm effect with a percentage inhibition of 66.10 ± 1.79. The methanolic extract of Cocos nucifera had the strongest inhibition on the production of quorum sensing (61.42 ± 0.28). In addition, the methanolic extract of Cocos nucifera roots showed the best cytotoxic effect on prostate cancer LNCaP cell lines (IC<sub>50</sub> = 26.98 ± 2.6 μg/mL) and Carica papaya on the PC-3 lines (IC<sub>50</sub> = 127.20 ± 5.99 μg/mL). The extracts were also rich in triterpenes and sterols. Conclusion: This study provides support for the ethnomedical use of Carica papaya and Cocos nucifera roots as an antimicrobial and anticancer.
文摘Bacterial cells rely on signaling molecules to communicate with others from the same species and induce certain genes in a process known as quorum sensing (QS). A common molecule is N-acyl homoserine lactone (AHL) which is responsible for the expression of virulence and other factors that allow the organisms to compete in a given environment. On the other hand, other bacteria produce certain enzymes such as AHL-lactonase that break down AHL molecules and prevent gene expression of these factors. The aim of this work was to examine the level of degradation of AHL molecules by AHL-lactonase in 62 Bacillus thuringiensis (Bt) strains isolated from Middle Tennessee, Mississippi, and Alabama. N-hexanoyl-homoserine lactone (C<sub>6</sub>-HSL) and N-3-oxo-hexanoyl homoserine lactone (3-oxo-C<sub>6</sub>-HSL), which cause Chromobacterium violaceum (CV026) to produce a purple pigment were tested at different concentrations to view the Bt lactonase activity. In addition, PCR was used to test for the presence of the lactonase gene. The results showed that among the 62 Bt strains, there were 58 that possessed the AHL-lactonase (aiiA) gene and 48 strains were able to degrade C<sub>6</sub>-HSL. At high concentrations of AHL, only 13 strains were able to completely degrade C6-HSL. In addition, degradation of 3-oxo-C<sub>6</sub>-HSL was weak compared to C<sub>6</sub>-HSL. The results also revealed that AHL lactonase was thermostable, and it was concluded that the level of degradation varies in Bt strains. Only 13 of the strains studied have potent inhibitory activity against C<sub>6</sub>-HSL, which may be good to be used in field applications to control agricultural pest.
文摘在无线传感器网络中,异步低占空比技术可以极大地降低能耗,但是由于节点的低占空比唤醒会造成极大的端到端数据时延。针对这个问题提出一种基于Quorum的异步自适应低占空比路由算法ORDA(Optimal-Reliable delay routing algorithm for low duty cycle WSNs based on Quorum),将异步占空比网络和实际链路模型相结合,在异步占空比网络中节点在不同时刻的邻居发现延迟也在不断变化。首先为每个节点根据网络负载选择自身的Quorum类型,并利用Quorum特性来计算邻居节点的重叠时隙个数;然后根据链路质量进一步计算出这一跳范围内邻居节点间的成功转发预期值,并在即将唤醒的节点中选择更可靠的节点转发数据。仿真实验证明,该算法不仅能够降低端到端延迟,而且能获得很好的转发成功率。
文摘针对无线传感器网络占空比MAC协议在链路或节点失效环境下易导致严重的能量浪费问题,提出了一种基于Grid Quorum的异步低占空比M AC协议-AGQ-M AC(Asynchronous Grid Quorum M AC).AGQ-M AC采用Grid Quorum组分配信道,通过动态调配Quorum比率得到了节点的最优占空比,并在邻节点发现过程中采用双前导序文抽样来监测信道状态,以减少节点唤醒时间从而达到能量高效.仿真实验结果表明,与其他占空比MAC协议相比,AGQ-MAC延长了网络生存时间,降低了网络能耗,提高了邻发现数据传输率,同时保持较低的平均邻发现延迟.
