Red blood cell(RBC)aggregation as well as their deformation significantly affects blood microrheology.These processes depend on various factors,one of which is concentration of the nitric oxide,one of the main signali...Red blood cell(RBC)aggregation as well as their deformation significantly affects blood microrheology.These processes depend on various factors,one of which is concentration of the nitric oxide,one of the main signaling molecule in the bloodstream.The purpose of this study was to investigate the effect of nitric oxide on the microrheological properties of red blood cells(RBCs)in RBC samples of various media after the addition of nitric oxide donor sodium nitroprusside in vitro.Microrheological properties were measured using laser aggregometer and ektacytometer based on diffuse light scattering and diffraction of laser light on a suspension of RBCs,respectively.The study found that heparin-stabilized blood showed increased RBC aggregation and deformation with sodium nitroprusside concentrations of 100,and 200M,while EDTA-stabilized blood showed slightly decreased aggregation and unchanged deformation.With washed RBCs in dextran solution,the addition of sodium nitroprusside(in the concentrations of 100,and 200M)resulted in decreased aggregation and increased deformation.These-ndings aid in our understanding of nitric oxide's effect on RBC microrheological properties.展开更多
A two-dinmnsional red blood cell （RBC） membrane model based on elastic and Euler- Bernoulli beam theories is introduced in the frame of immersed boundary-lattice Boltz- mann method （IB-LBM）. The effect of the flex...A two-dinmnsional red blood cell （RBC） membrane model based on elastic and Euler- Bernoulli beam theories is introduced in the frame of immersed boundary-lattice Boltz- mann method （IB-LBM）. The effect of the flexible membrane is handled by the immersed boundary method in which the stress exerted by the RBC on the ambient fluid is spread onto the collocated grid points near the boundary. The fluid dynamics is obtained by solving the discrete lattice Boltzmann equation. A ＂ghost shape＂, to which the RBC returns when restoring, is introduced by prescribing a bending force along the bound- ary. Numerical examples involving tumbling, tank-treading and RBC aggregation in shear flow and deformation and restoration in poiseuille flow are presented to verify the method and illustrate its efficiency. As an application of the present method, a ten-RBC colony being compressed through a stenotic microvessel is studied focusing the cell-cell interaction strength. Quantitative comparisons of the pressure and velocity on speci- fled microvessel interfaces are made between each aggregation case. It reveals that the stronger aggregation may lead to more resistance against blood flow and result in higher pressure difference at the stenosis.展开更多
基金supported by the Russian Science Foundation grant(No.22-15-00120)supported by the grant(No.21-2-10-59-1)from the Foundation for the Development of Theoretical Physics and Mathematics BASIS.
文摘Red blood cell(RBC)aggregation as well as their deformation significantly affects blood microrheology.These processes depend on various factors,one of which is concentration of the nitric oxide,one of the main signaling molecule in the bloodstream.The purpose of this study was to investigate the effect of nitric oxide on the microrheological properties of red blood cells(RBCs)in RBC samples of various media after the addition of nitric oxide donor sodium nitroprusside in vitro.Microrheological properties were measured using laser aggregometer and ektacytometer based on diffuse light scattering and diffraction of laser light on a suspension of RBCs,respectively.The study found that heparin-stabilized blood showed increased RBC aggregation and deformation with sodium nitroprusside concentrations of 100,and 200M,while EDTA-stabilized blood showed slightly decreased aggregation and unchanged deformation.With washed RBCs in dextran solution,the addition of sodium nitroprusside(in the concentrations of 100,and 200M)resulted in decreased aggregation and increased deformation.These-ndings aid in our understanding of nitric oxide's effect on RBC microrheological properties.
文摘A two-dinmnsional red blood cell （RBC） membrane model based on elastic and Euler- Bernoulli beam theories is introduced in the frame of immersed boundary-lattice Boltz- mann method （IB-LBM）. The effect of the flexible membrane is handled by the immersed boundary method in which the stress exerted by the RBC on the ambient fluid is spread onto the collocated grid points near the boundary. The fluid dynamics is obtained by solving the discrete lattice Boltzmann equation. A ＂ghost shape＂, to which the RBC returns when restoring, is introduced by prescribing a bending force along the bound- ary. Numerical examples involving tumbling, tank-treading and RBC aggregation in shear flow and deformation and restoration in poiseuille flow are presented to verify the method and illustrate its efficiency. As an application of the present method, a ten-RBC colony being compressed through a stenotic microvessel is studied focusing the cell-cell interaction strength. Quantitative comparisons of the pressure and velocity on speci- fled microvessel interfaces are made between each aggregation case. It reveals that the stronger aggregation may lead to more resistance against blood flow and result in higher pressure difference at the stenosis.
