Objective: To observe changes in RBC count, changes, RBC morphology, and serum potassium during compressed transfusion of WBC-removal whole blood. Methods: Prepared human WBC-removal whole blood and connected transf...Objective: To observe changes in RBC count, changes, RBC morphology, and serum potassium during compressed transfusion of WBC-removal whole blood. Methods: Prepared human WBC-removal whole blood and connected transfusion apparatus with different sizes of intravenous detaining needles (18G, 20G, 22G and 24G). Observed RBC count and serum potassium concentration under different pressures (100 mmHg, 200 mmHg, and 300 mmHg) as blood flowed out of the pinhead end of the intravenous detaining needle. Samples obtained with the 20G needle were smeared on glass slides, and RBC morphologic changes were observed under an oil immersion lens. Results: RBC count and serum potassium changed slightly under different pressures with different sizes of intravenous detaining needles as blood flowed through the transfusion apparatus. In addition, the observation of blood samples under a common light microscope revealed that coarse- prick, oblong, and spindle cell counts in the visual fields increased gradually as the pressure increased. Additionally, a portion of cells had undergone splintering. Conclusions: While applying 18G, 20G, 22G and 24G intravenous detaining needles for blood transfusion under less than 300 mmHg of pressure, no significant RBC count change was found in blood samples in the short term. However, there were significant RBC morphologic changes. The results could offer more basis to ensure the clinical safetv of oatients undereoing blood transfilsion.展开更多
目的了解复方电解质注射液洗涤的术中回收(IBS)红细胞回输患者时间<3 d血浆Hb及RBC的变化情况。方法选择46名择期行骨科手术且做IBS的患者,分别以生理盐水(NS组,n=24)和复方电解质液(MEI组,n=22)洗涤IBS红细胞,于术前、术(输)后即刻...目的了解复方电解质注射液洗涤的术中回收(IBS)红细胞回输患者时间<3 d血浆Hb及RBC的变化情况。方法选择46名择期行骨科手术且做IBS的患者,分别以生理盐水(NS组,n=24)和复方电解质液(MEI组,n=22)洗涤IBS红细胞,于术前、术(输)后即刻、术(输)后1-3 d取2组患者静脉血2 mL/人(份),检测Hb、RBC并记录术中2组失血量、回收红细胞净输入量和术后引流量等。结果2组患者术前一般情况、手术时间、回收红细胞离体时间、术中失血量、回收红细胞净输入量相近(P>0.05)。术前、术(输)后即刻及术(输)后1-3 d Hb(g/L),组内比较:NS组术前vs术(输)后即刻为121.4±14.5 vs 109.0±18.3、术(输)后1 d vs术(输)后2 d为110.0±16.7 vs 102.8±17.7、术(输)后2-3 d vs术(输)后即刻为102.8±17.7 vs 101.7±15.6 vs 109.0±18.3(P<0.01);术(输)后即刻vs术(输)后1 d为109.0±18.3 vs 110.0±16.7、术(输)后2 d vs术(输)后3 d为102.8±17.7 vs 101.7±15.6(P>0.05),MEI组术前vs术(输)后即刻为127.3±14.0 vs 112.0±14.4、术后(输)即刻vs术(输)后1 d为112.0±14.4 vs 119.8±15.7、术(输)后1 d vs术(输)后2 d为119.8±15.7 vs 106.6±16.3(P<0.01);术(输)后2 d vs术(输)后3 d为106.6±16.3 vs 107.3±15.0、术(输)后2-3 d vs术(输)后即刻为106.6±16.3 vs 107.3±15.0 vs 112.0±14.4(P>0.05);组间比较:NS与MEI组术(输)后1 d为110.0±16.7 vs 119.8±15.7(P<0.05)、其余时间的检测值2组相近(P>0.05)。术前、术(输)后即刻、术(输)后1-3 d RBC(×10^9/L),组内比较:NS组分别为术前vs术(输)后即刻为3.91±0.49 vs 3.52±0.63、术(输)1 d vs术(输)2 d为3.56±0.59 vs 3.31±0.56、术(输)后2-3 d vs术(输)后即刻为3.31±0.56 vs 3.30±0.53 vs 3.52±0.63(P<0.01);术(输)后即刻vs术(输)后1 d为3.52±0.63 vs 3.56±0.59、术(输)后2 d vs术(输)后3 d为3.31±0.56 vs 3.30±0.53(P>0.05),MEI组分别为术前vs术(输)后即刻为4.13±0.43 vs 3.67±0.47、术(输)后即刻vs术(输)1 d为3.67±0.47 vs 3.92±0.55、术(输)后1 d vs术(输)2 d后为3.92±0.55 vs 3.53±0.55(P<0.01),术(输)后2 d vs术(输)后3 d为3.53±0.55 vs 3.56±0.50、术(输)后2-3 d vs术(输)后即刻为3.53±0.55 vs 3.56±0.50 vs 3.67±0.47(P>0.05),组间比较:NS与MEI组术(输)后1 d为3.56±0.59 vs 3.92±0.55(P<0.05),其余时间的检测值2组相近(P>0.05)。结论复方电解质注射液可以延缓IBS红细胞回输体内后患者Hb和RBC下降速度。展开更多
基金supported by the Shanxi Science and Technology Development Fund(No.200233)
文摘Objective: To observe changes in RBC count, changes, RBC morphology, and serum potassium during compressed transfusion of WBC-removal whole blood. Methods: Prepared human WBC-removal whole blood and connected transfusion apparatus with different sizes of intravenous detaining needles (18G, 20G, 22G and 24G). Observed RBC count and serum potassium concentration under different pressures (100 mmHg, 200 mmHg, and 300 mmHg) as blood flowed out of the pinhead end of the intravenous detaining needle. Samples obtained with the 20G needle were smeared on glass slides, and RBC morphologic changes were observed under an oil immersion lens. Results: RBC count and serum potassium changed slightly under different pressures with different sizes of intravenous detaining needles as blood flowed through the transfusion apparatus. In addition, the observation of blood samples under a common light microscope revealed that coarse- prick, oblong, and spindle cell counts in the visual fields increased gradually as the pressure increased. Additionally, a portion of cells had undergone splintering. Conclusions: While applying 18G, 20G, 22G and 24G intravenous detaining needles for blood transfusion under less than 300 mmHg of pressure, no significant RBC count change was found in blood samples in the short term. However, there were significant RBC morphologic changes. The results could offer more basis to ensure the clinical safetv of oatients undereoing blood transfilsion.
文摘目的了解复方电解质注射液洗涤的术中回收(IBS)红细胞回输患者时间<3 d血浆Hb及RBC的变化情况。方法选择46名择期行骨科手术且做IBS的患者,分别以生理盐水(NS组,n=24)和复方电解质液(MEI组,n=22)洗涤IBS红细胞,于术前、术(输)后即刻、术(输)后1-3 d取2组患者静脉血2 mL/人(份),检测Hb、RBC并记录术中2组失血量、回收红细胞净输入量和术后引流量等。结果2组患者术前一般情况、手术时间、回收红细胞离体时间、术中失血量、回收红细胞净输入量相近(P>0.05)。术前、术(输)后即刻及术(输)后1-3 d Hb(g/L),组内比较:NS组术前vs术(输)后即刻为121.4±14.5 vs 109.0±18.3、术(输)后1 d vs术(输)后2 d为110.0±16.7 vs 102.8±17.7、术(输)后2-3 d vs术(输)后即刻为102.8±17.7 vs 101.7±15.6 vs 109.0±18.3(P<0.01);术(输)后即刻vs术(输)后1 d为109.0±18.3 vs 110.0±16.7、术(输)后2 d vs术(输)后3 d为102.8±17.7 vs 101.7±15.6(P>0.05),MEI组术前vs术(输)后即刻为127.3±14.0 vs 112.0±14.4、术后(输)即刻vs术(输)后1 d为112.0±14.4 vs 119.8±15.7、术(输)后1 d vs术(输)后2 d为119.8±15.7 vs 106.6±16.3(P<0.01);术(输)后2 d vs术(输)后3 d为106.6±16.3 vs 107.3±15.0、术(输)后2-3 d vs术(输)后即刻为106.6±16.3 vs 107.3±15.0 vs 112.0±14.4(P>0.05);组间比较:NS与MEI组术(输)后1 d为110.0±16.7 vs 119.8±15.7(P<0.05)、其余时间的检测值2组相近(P>0.05)。术前、术(输)后即刻、术(输)后1-3 d RBC(×10^9/L),组内比较:NS组分别为术前vs术(输)后即刻为3.91±0.49 vs 3.52±0.63、术(输)1 d vs术(输)2 d为3.56±0.59 vs 3.31±0.56、术(输)后2-3 d vs术(输)后即刻为3.31±0.56 vs 3.30±0.53 vs 3.52±0.63(P<0.01);术(输)后即刻vs术(输)后1 d为3.52±0.63 vs 3.56±0.59、术(输)后2 d vs术(输)后3 d为3.31±0.56 vs 3.30±0.53(P>0.05),MEI组分别为术前vs术(输)后即刻为4.13±0.43 vs 3.67±0.47、术(输)后即刻vs术(输)1 d为3.67±0.47 vs 3.92±0.55、术(输)后1 d vs术(输)2 d后为3.92±0.55 vs 3.53±0.55(P<0.01),术(输)后2 d vs术(输)后3 d为3.53±0.55 vs 3.56±0.50、术(输)后2-3 d vs术(输)后即刻为3.53±0.55 vs 3.56±0.50 vs 3.67±0.47(P>0.05),组间比较:NS与MEI组术(输)后1 d为3.56±0.59 vs 3.92±0.55(P<0.05),其余时间的检测值2组相近(P>0.05)。结论复方电解质注射液可以延缓IBS红细胞回输体内后患者Hb和RBC下降速度。