RET(rearranged during transfection)原癌基因重排及突变与甲状腺乳头状癌(PTC)密切相关,它在调节细胞增殖、迁移、分化过程中具有重要意义。RET原癌基因及其编码蛋白的结构已经明确,此基因可介导多条信号转导通路。研究发现,RET/PTC...RET(rearranged during transfection)原癌基因重排及突变与甲状腺乳头状癌(PTC)密切相关,它在调节细胞增殖、迁移、分化过程中具有重要意义。RET原癌基因及其编码蛋白的结构已经明确,此基因可介导多条信号转导通路。研究发现,RET/PTC重排与甲状腺的放射暴露、促炎性反应以及肿瘤的预后有关。针对此靶基因,目前有多种基因治疗方法正在研究过程中,并有望成为肿瘤治疗的新的突破口。展开更多
Objective The aim of this study was to investigate the effects of rearranged during transfection(RET)mutation on the expressions of calcitonin(CTn)and procalcitonin(PCT)in sporadic medullary thyroid carcinoma(SMTC).Me...Objective The aim of this study was to investigate the effects of rearranged during transfection(RET)mutation on the expressions of calcitonin(CTn)and procalcitonin(PCT)in sporadic medullary thyroid carcinoma(SMTC).Methods RET mutation was detected by polymerase chain reaction direct sequencing in 64 cases of SMTC,and the expression levels of CTn and PCT in SMTC tissues were detected using the immunohistochemical streptavidin-perosidase(SP)method.The effect of RET mutations on the expression of CTn and PCT along with its relationship with clinicopathological parameters were analyzed.Results The expression rates of CTn and PCT in SMTC tissues were 90.6%(58/64)and 67.2%(43/64),respectively.CTn and PCT expression were found to be associated with tumor size and lymph node metastasis(P<0.05)but not with gender,age,or tumor capsule invasion(P>0.05).There was a significant correlation between CTn and PCT expression(r=0.269,P=0.041),and the intensity of positive CTn expression was positively correlated with RET mutation(r=0.507,P=0.000).However,PCT expression was not associated with RET mutation(r=0.188,P=0.136).Conclusion High expression of CTn and PCT was associated with the progression of medullary carcinoma,and the intensity of CTn expression was associated with RET mutation.PCT may provide valuable information for the diagnosis and prognosis of SMTC.展开更多
文摘RET(rearranged during transfection)原癌基因重排及突变与甲状腺乳头状癌(PTC)密切相关,它在调节细胞增殖、迁移、分化过程中具有重要意义。RET原癌基因及其编码蛋白的结构已经明确,此基因可介导多条信号转导通路。研究发现,RET/PTC重排与甲状腺的放射暴露、促炎性反应以及肿瘤的预后有关。针对此靶基因,目前有多种基因治疗方法正在研究过程中,并有望成为肿瘤治疗的新的突破口。
基金Supported by a grant from the Gansu Provincial Funding for Health Research(No.GSWSKY2018-13).
文摘Objective The aim of this study was to investigate the effects of rearranged during transfection(RET)mutation on the expressions of calcitonin(CTn)and procalcitonin(PCT)in sporadic medullary thyroid carcinoma(SMTC).Methods RET mutation was detected by polymerase chain reaction direct sequencing in 64 cases of SMTC,and the expression levels of CTn and PCT in SMTC tissues were detected using the immunohistochemical streptavidin-perosidase(SP)method.The effect of RET mutations on the expression of CTn and PCT along with its relationship with clinicopathological parameters were analyzed.Results The expression rates of CTn and PCT in SMTC tissues were 90.6%(58/64)and 67.2%(43/64),respectively.CTn and PCT expression were found to be associated with tumor size and lymph node metastasis(P<0.05)but not with gender,age,or tumor capsule invasion(P>0.05).There was a significant correlation between CTn and PCT expression(r=0.269,P=0.041),and the intensity of positive CTn expression was positively correlated with RET mutation(r=0.507,P=0.000).However,PCT expression was not associated with RET mutation(r=0.188,P=0.136).Conclusion High expression of CTn and PCT was associated with the progression of medullary carcinoma,and the intensity of CTn expression was associated with RET mutation.PCT may provide valuable information for the diagnosis and prognosis of SMTC.
