[Objective] The aim was to provide information for the breeding of wheat in China.[Method] 64 wheat cultivars from Australia were analyzed by SDS-PAGE to determine their high molecular weight（HMW） glutenin subunit c...[Objective] The aim was to provide information for the breeding of wheat in China.[Method] 64 wheat cultivars from Australia were analyzed by SDS-PAGE to determine their high molecular weight（HMW） glutenin subunit combinations.Then,the cultivars had good HMW-GS were screened.[Result] A total of nine alleles were identified at Glu-1.Glu-A1 encoded two subunits,1 and null subunit,and 1 was the major type,the frequency was 59.37%;Glu-B1 encoded five subunits,7,7＋8,7＋9,14＋15,17＋18,and 7＋8 was the major type,the frequency was 56.2%;Glu-D1 encoded two subunits,2＋12,5＋10,and 2＋12 was the major type,the frequency was 70.3%.Furthermore,12 subunit combinations were detected,and the composition of ＂1,2＋12,7＋8＂ was the major type.The quality scores of these cultivars ranged from 4 to 10,with an average of 7.4.[Conclusion] The quality of these varieties was good.展开更多
To investigate the effect of high molecular weight glutenin subunits (HMW-GS) 5+10 inhigh quality spring wheat, four near-isogenic lines (NILs) developed by 6 consecutivebackcrosses sharing the same genetic background...To investigate the effect of high molecular weight glutenin subunits (HMW-GS) 5+10 inhigh quality spring wheat, four near-isogenic lines (NILs) developed by 6 consecutivebackcrosses sharing the same genetic background and differing only at the Glu-D1 locusin each pair were employed in this study. The results showed that gluten indexes were allincreased by 3.1-27.7% (P<0.01) after the 5+10 subunits introduced into the fourcultivars. Their stability time was increased by 128, 104, 233 and 36%, where the maximumresistance raised by 130, 56.04, 95.10 and 16.33% in Kefeng6, Longmai20, Longfumai10and Xiaobingmai33, respectively.展开更多
To determine the effect of 6 ＋ 8 and 1.5 ＋ 10 HMW-GS of synthetic hexaploid wheat （SHW） on main quality parameters of wheat, a set of recombinant inbred lines （RILs） derived from the cross between a SHW with N,...To determine the effect of 6 ＋ 8 and 1.5 ＋ 10 HMW-GS of synthetic hexaploid wheat （SHW） on main quality parameters of wheat, a set of recombinant inbred lines （RILs） derived from the cross between a SHW with N, 6 ＋ 8, 1.5 ＋ 10 HMW-GS and a cultivar Chuanyu 12-1 （CY 12-1） with 1, 7 ＋ 8, 2 ＋ 12 were planted in three environments in 2005 and 2006 and totally 16 quality parameters were tested for each line. Significant differences in all tested quality parameters but flour yield were observed between the two parents. The mean values of the RILs were intermediate to the parents for grain and protein parameters and some farinograph parameters, flour water absorption （FWA）, and farinograph softening （SOF） but beyond parents at dough stability time （DST）, breakdown time （BRT）, quality number （QN）, noodle score （NS）, and loaf volume （LOV）. All of the quality traits, especially in grain hardness （GH）, zeleny sedimentation volume （SED）, and most of farinograph parameters had significant difference between the different HMW-GS components. The effects of different alleles of HMW-GS at same locus （Glu-A1 or Glu-B1 or GIu-D1） on the different quality parameters were also different and affected by the other two loci. For most of parameters tested, 6 ＋ 8 was better than 7 ＋ 8 and there was no difference between 1.5 ＋ 10 and 2 ＋ 12. End-use quality was greatly influenced by components of HMW-GS. The components of 1, 6 ＋ 8, 1.5 ＋ 10 had the highest LOV and bread score （BS） values, whereas the components of 1, 7＋ 8 and 1.5 ＋ 10 had the highest NS values. Noodle score performed a positive linear relationship with falling number （FN） and its relationships to other quality parameters were affected by environments. Loaf volume had a significant negative relationship to SOF and positive associations with most of quality parameters. It could be concluded that HMW-GS 6＋ 8 from SHW had better overall quality characteristics than 7 ＋ 8, whereas the effects of 1.5 ＋ 10 on quality was different in respect to quality parameters and the HMW-GS components. Synthetic hexaploid wheat with subunits 6 ＋ 8 and 1.5 ＋ 10 had the potentials to improve the end-use quality of wheat cultivars.展开更多
Hand-extended noodle, a special kind of noodle, requires particular quality flour to make it. High molecular weight glutenin subunits （HMW-GS） in wheat are important protein subunits, which affect flour quality. To ...Hand-extended noodle, a special kind of noodle, requires particular quality flour to make it. High molecular weight glutenin subunits （HMW-GS） in wheat are important protein subunits, which affect flour quality. To improve breeding and selection efficiency of wheat varieties which are used in making hand-extended noodle, 100 spring wheat varieties were selected to study the importance of HMW-GS on noodle quality score indexes such as color, appearance, taste agreeability, toughness, stickiness, smoothness, taste, and total score, through methods of quantity theory and statistic evaluation. It was shown that the hand-extended noodle quality score of HMW-GS 1, 2°, N, 7, 7 ＋ 8, 17 ＋ 18, 22, 2 ＋ 10, 2 ＋ 11, 2＋ 12, 5 ＋ 10, and 10 was 5.40, 5.35, 0, 2.55, 2.56, 9.19, 0.05, 0.15, 1.49, 1.14, 10.00, and 5.14, respectively. The score system for hand-extended noodle quality based on HMW-GS index included eight multiple linear regression equations （R^2 〉 0.98）. Hence, using the HMW-GS composition, the eight hand-extended noodle quality indexes would be forecasted exactly. Results indicated that ideal subunit compositions of HMW-GS for this special usage were composition 1, 17 ＋ 18, 5 ＋ 10, or composition 2°, 17 ＋ 18, 5 ＋ 10. This standard could be used on variety selection in the early generation of breeding crosses. HMW-GS 2 ＋ 10, 2 ＋ 11, and 2 ＋ 12 were the least desirable subunits for hand-extended noodle, which should be avoided in wheat variety selection aimed for hand-extended noodle flour use.展开更多
High-molecular-weight glutenin subunits （HMW-GSs）, one class of seed storage proteins in wheat, play an important role in determining bread-making quality of flour. More and more proves support that HMW-GS- 1Bx 14 a...High-molecular-weight glutenin subunits （HMW-GSs）, one class of seed storage proteins in wheat, play an important role in determining bread-making quality of flour. More and more proves support that HMW-GS- 1Bx 14 and - 1Bx 15 subunits are strongly positively associated with good bread-making and excellent noodle-making quality. The two subunits are encoded by two genes, Glu-1Bx14 and Glu-1Bx15, which are tightly linked and located on the 1BL. Protein assay by SDS-PAGE indicated that the expression of Glu-1Bx14 gene was always much stronger than that of Glu-1By15 in the same variety. But, variation of expression level for Glu-1By15 gene existed among varieties, such as in Xiaoyan 54, Xiaoyan 6, Yanzhan 1 and Shanyou 225. We also investigated the transcription difference of Glu-1By14 and Glu-1By15 genes in Xiaoyan 54 and Shanyou 225 by semi-quantitative RT-PCR method. The Glu-1By14 always transcripts much more than the Glu-1By15. This was basically consistent with the translation difference between the two genes. Promoters of 1Bx14, 1By15, 1By8, 1Dx2 and 1Dy12 were cloned from Xiaoyan 54, Chinese Spring and Aegilops tauschii. Sequence analysis indicated that the HMW-GS genes had high homology at their promoter regions. However, significant difference existed between sequence of 1Bx14 promoter and those of other HMW-GS genes. The transient expression experiment showed that the promoter of 1By15 has lower activity than that of 1Bx14, which was consistent with their transcription level of the two genes in varieties. In addition, transient expression of the gus driven by the promoter （P2） of HMW-GS 1Dx2 gene was higher than by other HMW-GS promoters. Therefore, we constructed 1By15 gene expression vector driven by the 1Dx2 promoter, and transformed the 1By15 gene into wheat commercial variety, Jimai 20 by pollen tube method. Of 45 independent transgenic lines identified by PCR, 3 were confirmed to contain the HMW-GS 1By15 gene via Southern hybridization. The delivered 1By15 gene expressed the expected HMW-GS protein in the seeds of transgenic plants.展开更多
The composition of high molecular weight glutenin subunits( HMW-GS) was analyzed using SDS-PAGE with 70 Longdong dryland winter wheat germplasm resources as experimental materials.The results showed that Longdong dryl...The composition of high molecular weight glutenin subunits( HMW-GS) was analyzed using SDS-PAGE with 70 Longdong dryland winter wheat germplasm resources as experimental materials.The results showed that Longdong dryland winter wheat germplasm resources had N,7 + 8,2 + 12 as the dominant HMW-GS composition( 42.86%),while the dominant HMW-GS composition in the introduced germplasms was N,7 + 9,2 + 12( 36.84%).On the Glu-B1 locus of Longdong dryland germplasms,subunit 7 + 8 had the highest frequency,accounting for 65.71%,and on the Glu-B1 locus of the introduced germplasms,subunit7 + 9 had the highest frequency,accounting for 57.89%.Whether subunit 7 + 8 is related to drought resistance in varieties still needs further study.On Glu-D1 locus,high-quality subunit 5 + 10 appeared in the introduced resources for 5 times,accounting for 13.16% of all the introduced lines,and appeared in Longdong dryland winter wheat germplasm resources for 2 times,only accounting for 2.86% of all the resources,suggesting that Longdong dryland germplasms lack high-quality subunits and need further improvement.展开更多
利用普通小麦品种藁城8901和PH85-16按单粒传方法构建的重组自交系群体F6(RIL-6)共112个家系,研究了影响小麦粉及面片色泽的主要因素。结果表明:硬度、吸水率、湿面筋、干面筋、蛋白质含量,与小麦粉白度、L*值及鲜面片0 h L*值呈负相关...利用普通小麦品种藁城8901和PH85-16按单粒传方法构建的重组自交系群体F6(RIL-6)共112个家系,研究了影响小麦粉及面片色泽的主要因素。结果表明:硬度、吸水率、湿面筋、干面筋、蛋白质含量,与小麦粉白度、L*值及鲜面片0 h L*值呈负相关,与小麦粉及鲜面片0 h的a*和b*值呈正相关;叶黄素和PPO活性,与小麦粉及鲜面片0 h L*值呈负相关,与b*值呈正相关;面筋指数和稳定时间,与小麦粉及面片的L*值呈正相关;淀粉糊化性状的几个参数,与小麦粉白度、L*值及鲜面片0 h L*值呈正相关,与小麦粉及鲜面片0 h的a*值呈负相关。由结果看出在小麦粉及面片色泽性状的选育过程中,对蛋白质和淀粉等组分含量进行选择的同时,也要注意内部特性的改良。选择面筋指数高、叶黄素和PPO活性低、淀粉糊化黏度高的株系,以满足人们对亮白色食品的需求。展开更多
基金Supported by National wheat industry,modern agriculture technolo-gy system (CARS-03)China Ministry of Science and Technologyunder Contract (2009CB118300)~~
文摘[Objective] The aim was to provide information for the breeding of wheat in China.[Method] 64 wheat cultivars from Australia were analyzed by SDS-PAGE to determine their high molecular weight（HMW） glutenin subunit combinations.Then,the cultivars had good HMW-GS were screened.[Result] A total of nine alleles were identified at Glu-1.Glu-A1 encoded two subunits,1 and null subunit,and 1 was the major type,the frequency was 59.37%;Glu-B1 encoded five subunits,7,7＋8,7＋9,14＋15,17＋18,and 7＋8 was the major type,the frequency was 56.2%;Glu-D1 encoded two subunits,2＋12,5＋10,and 2＋12 was the major type,the frequency was 70.3%.Furthermore,12 subunit combinations were detected,and the composition of ＂1,2＋12,7＋8＂ was the major type.The quality scores of these cultivars ranged from 4 to 10,with an average of 7.4.[Conclusion] The quality of these varieties was good.
基金financially supported by the National Natural Science Foundation of China(39770461)the Science and Technology Department of Heilongjiang Province,China.
文摘To investigate the effect of high molecular weight glutenin subunits (HMW-GS) 5+10 inhigh quality spring wheat, four near-isogenic lines (NILs) developed by 6 consecutivebackcrosses sharing the same genetic background and differing only at the Glu-D1 locusin each pair were employed in this study. The results showed that gluten indexes were allincreased by 3.1-27.7% (P<0.01) after the 5+10 subunits introduced into the fourcultivars. Their stability time was increased by 128, 104, 233 and 36%, where the maximumresistance raised by 130, 56.04, 95.10 and 16.33% in Kefeng6, Longmai20, Longfumai10and Xiaobingmai33, respectively.
基金the Sichuan Provincial Youth Foundation (04ZQ026-009)National 863 Pro-gram of China (2006AA10Z1C6)+1 种基金National Natural Science Foundation of China (30771338)Na-tional Key Technology R&D Program of China(2006BAD01A02, 2006BAD13B02)
文摘To determine the effect of 6 ＋ 8 and 1.5 ＋ 10 HMW-GS of synthetic hexaploid wheat （SHW） on main quality parameters of wheat, a set of recombinant inbred lines （RILs） derived from the cross between a SHW with N, 6 ＋ 8, 1.5 ＋ 10 HMW-GS and a cultivar Chuanyu 12-1 （CY 12-1） with 1, 7 ＋ 8, 2 ＋ 12 were planted in three environments in 2005 and 2006 and totally 16 quality parameters were tested for each line. Significant differences in all tested quality parameters but flour yield were observed between the two parents. The mean values of the RILs were intermediate to the parents for grain and protein parameters and some farinograph parameters, flour water absorption （FWA）, and farinograph softening （SOF） but beyond parents at dough stability time （DST）, breakdown time （BRT）, quality number （QN）, noodle score （NS）, and loaf volume （LOV）. All of the quality traits, especially in grain hardness （GH）, zeleny sedimentation volume （SED）, and most of farinograph parameters had significant difference between the different HMW-GS components. The effects of different alleles of HMW-GS at same locus （Glu-A1 or Glu-B1 or GIu-D1） on the different quality parameters were also different and affected by the other two loci. For most of parameters tested, 6 ＋ 8 was better than 7 ＋ 8 and there was no difference between 1.5 ＋ 10 and 2 ＋ 12. End-use quality was greatly influenced by components of HMW-GS. The components of 1, 6 ＋ 8, 1.5 ＋ 10 had the highest LOV and bread score （BS） values, whereas the components of 1, 7＋ 8 and 1.5 ＋ 10 had the highest NS values. Noodle score performed a positive linear relationship with falling number （FN） and its relationships to other quality parameters were affected by environments. Loaf volume had a significant negative relationship to SOF and positive associations with most of quality parameters. It could be concluded that HMW-GS 6＋ 8 from SHW had better overall quality characteristics than 7 ＋ 8, whereas the effects of 1.5 ＋ 10 on quality was different in respect to quality parameters and the HMW-GS components. Synthetic hexaploid wheat with subunits 6 ＋ 8 and 1.5 ＋ 10 had the potentials to improve the end-use quality of wheat cultivars.
文摘Hand-extended noodle, a special kind of noodle, requires particular quality flour to make it. High molecular weight glutenin subunits （HMW-GS） in wheat are important protein subunits, which affect flour quality. To improve breeding and selection efficiency of wheat varieties which are used in making hand-extended noodle, 100 spring wheat varieties were selected to study the importance of HMW-GS on noodle quality score indexes such as color, appearance, taste agreeability, toughness, stickiness, smoothness, taste, and total score, through methods of quantity theory and statistic evaluation. It was shown that the hand-extended noodle quality score of HMW-GS 1, 2°, N, 7, 7 ＋ 8, 17 ＋ 18, 22, 2 ＋ 10, 2 ＋ 11, 2＋ 12, 5 ＋ 10, and 10 was 5.40, 5.35, 0, 2.55, 2.56, 9.19, 0.05, 0.15, 1.49, 1.14, 10.00, and 5.14, respectively. The score system for hand-extended noodle quality based on HMW-GS index included eight multiple linear regression equations （R^2 〉 0.98）. Hence, using the HMW-GS composition, the eight hand-extended noodle quality indexes would be forecasted exactly. Results indicated that ideal subunit compositions of HMW-GS for this special usage were composition 1, 17 ＋ 18, 5 ＋ 10, or composition 2°, 17 ＋ 18, 5 ＋ 10. This standard could be used on variety selection in the early generation of breeding crosses. HMW-GS 2 ＋ 10, 2 ＋ 11, and 2 ＋ 12 were the least desirable subunits for hand-extended noodle, which should be avoided in wheat variety selection aimed for hand-extended noodle flour use.
文摘High-molecular-weight glutenin subunits （HMW-GSs）, one class of seed storage proteins in wheat, play an important role in determining bread-making quality of flour. More and more proves support that HMW-GS- 1Bx 14 and - 1Bx 15 subunits are strongly positively associated with good bread-making and excellent noodle-making quality. The two subunits are encoded by two genes, Glu-1Bx14 and Glu-1Bx15, which are tightly linked and located on the 1BL. Protein assay by SDS-PAGE indicated that the expression of Glu-1Bx14 gene was always much stronger than that of Glu-1By15 in the same variety. But, variation of expression level for Glu-1By15 gene existed among varieties, such as in Xiaoyan 54, Xiaoyan 6, Yanzhan 1 and Shanyou 225. We also investigated the transcription difference of Glu-1By14 and Glu-1By15 genes in Xiaoyan 54 and Shanyou 225 by semi-quantitative RT-PCR method. The Glu-1By14 always transcripts much more than the Glu-1By15. This was basically consistent with the translation difference between the two genes. Promoters of 1Bx14, 1By15, 1By8, 1Dx2 and 1Dy12 were cloned from Xiaoyan 54, Chinese Spring and Aegilops tauschii. Sequence analysis indicated that the HMW-GS genes had high homology at their promoter regions. However, significant difference existed between sequence of 1Bx14 promoter and those of other HMW-GS genes. The transient expression experiment showed that the promoter of 1By15 has lower activity than that of 1Bx14, which was consistent with their transcription level of the two genes in varieties. In addition, transient expression of the gus driven by the promoter （P2） of HMW-GS 1Dx2 gene was higher than by other HMW-GS promoters. Therefore, we constructed 1By15 gene expression vector driven by the 1Dx2 promoter, and transformed the 1By15 gene into wheat commercial variety, Jimai 20 by pollen tube method. Of 45 independent transgenic lines identified by PCR, 3 were confirmed to contain the HMW-GS 1By15 gene via Southern hybridization. The delivered 1By15 gene expressed the expected HMW-GS protein in the seeds of transgenic plants.
基金Supported by the Gansu Dryland Winter Wheat Germplasm Innovation and Application Engineering Research Center
文摘The composition of high molecular weight glutenin subunits( HMW-GS) was analyzed using SDS-PAGE with 70 Longdong dryland winter wheat germplasm resources as experimental materials.The results showed that Longdong dryland winter wheat germplasm resources had N,7 + 8,2 + 12 as the dominant HMW-GS composition( 42.86%),while the dominant HMW-GS composition in the introduced germplasms was N,7 + 9,2 + 12( 36.84%).On the Glu-B1 locus of Longdong dryland germplasms,subunit 7 + 8 had the highest frequency,accounting for 65.71%,and on the Glu-B1 locus of the introduced germplasms,subunit7 + 9 had the highest frequency,accounting for 57.89%.Whether subunit 7 + 8 is related to drought resistance in varieties still needs further study.On Glu-D1 locus,high-quality subunit 5 + 10 appeared in the introduced resources for 5 times,accounting for 13.16% of all the introduced lines,and appeared in Longdong dryland winter wheat germplasm resources for 2 times,only accounting for 2.86% of all the resources,suggesting that Longdong dryland germplasms lack high-quality subunits and need further improvement.
文摘利用普通小麦品种藁城8901和PH85-16按单粒传方法构建的重组自交系群体F6(RIL-6)共112个家系,研究了影响小麦粉及面片色泽的主要因素。结果表明:硬度、吸水率、湿面筋、干面筋、蛋白质含量,与小麦粉白度、L*值及鲜面片0 h L*值呈负相关,与小麦粉及鲜面片0 h的a*和b*值呈正相关;叶黄素和PPO活性,与小麦粉及鲜面片0 h L*值呈负相关,与b*值呈正相关;面筋指数和稳定时间,与小麦粉及面片的L*值呈正相关;淀粉糊化性状的几个参数,与小麦粉白度、L*值及鲜面片0 h L*值呈正相关,与小麦粉及鲜面片0 h的a*值呈负相关。由结果看出在小麦粉及面片色泽性状的选育过程中,对蛋白质和淀粉等组分含量进行选择的同时,也要注意内部特性的改良。选择面筋指数高、叶黄素和PPO活性低、淀粉糊化黏度高的株系,以满足人们对亮白色食品的需求。
