Prognostic role of ring finger and WD repeat domain 3 and immune cell infiltration in hepatocellular carcinoma

We have found that the expression of ring finger and WD repeat domain 3(RFWD3)is significantly higher in unpaired and paired hepatocellular carcinoma(HCC)tissues than in normal tissues.Moreover,this expression has a significant correlation with the infiltration level of 14 immune cell types and when the detected RFWD3 expression levels were grouped as high and low,a prominent difference was revealed for overall survival,disease-specific survival,and progression-free interval.Through statistical analysis(univariate Cox),we were also able to identify RFWD3 as an independent prognostic element for HCC,with RFWD3 having an ability to accurately predict HCC prognosis(area under the curve of 0.863).Finally,we have generated prognostic nomograms for probabilities of 1-,3-and 5-year overall survival in HCC via integrating the factors of age,pathologic stage,alpha-fetoprotein level,and RFWD3 expression.

Comparison of the Kobayashi-Royden and Sibony metrics on ring domains

In this paper we show that the Kobayashi-Royden metric and the Sibony metric are different on ring domains,i.e.,the difference of two concentric balls,in higher dimension.

RING finger and WD repeat domain 3 regulates proliferation and metastasis through the Wnt/β-catenin signalling pathways in hepatocellular carcinoma

BACKGROUND Hepatocellular carcinoma(HCC)exhibits high invasiveness and mortality rates,and the molecular mechanisms of HCC have gained increasing research interest.The abnormal DNA damage response has long been recognized as one of the important factors for tumor occurrence and development.Recent studies have shown the potential of the protein RING finger and WD repeat domain 3(RFWD3)that positively regulates p53 stability in response to DNA damage as a therapeutic target in cancers.AIM To investigate the relationship between HCC and RFWD3 in vitro and in vivo and explored the underlying molecular signalling transduction pathways.METHODS RFWD3 gene expression was analyzed in HCC tissues and adjacent normal tissues.Lentivirus was used to stably knockdown RFWD3 expression in HCC cell lines.After verifying the silencing efficiency,Celigo/cell cycle/apoptosis and MTT assays were used to evaluate cell proliferation and apoptosis.Subsequently,cell migration and invasion were assessed by wound healing and transwell assays.In addition,transduced cells were implanted subcutaneously and injected into the tail vein of nude mice to observe tumor growth and metastasis.Next,we used lentiviral-mediated rescue of RFWD3 shRNA to verify the phenotype.Finally,the microarray,ingenuity pathway analysis,and western blot analysis were used to analyze the regulatory network underlying HCC.RESULTS Compared with adjacent tissues,RFWD3 expression levels were significantly higher in clinical HCC tissues and correlated with tumor size and TNM stage(P<0.05),which indicated a poor prognosis state.RFWD3 silencing in BEL-7404 and HCC-LM3 cells increased apoptosis,decreased growth,and inhibited the migration in shRNAi cells compared with those in shCtrl cells(P<0.05).Furthermore,the in vitro results were supported by the findings of the in vivo experiments with the reduction of tumor cell invasion and migration.Moreover,the rescue of RFWD3 shRNAi resulted in the resumption of invasion and metastasis in HCC cell lines.Finally,gene expression profiling and subsequent experimental verification revealed that RFWD3 might influence the proliferation and metastasis of HCC via the Wnt/β-catenin signalling pathway.CONCLUSION We provide evidence for the expression and function of RFWD3 in HCC.RFWD3 affects the prognosis,proliferation,invasion,and metastasis of HCC by regulating the Wnt/β-catenin signalling pathway.

Goldbach's Problem in the Matrix Ring over a Principal Ideal Domain

In this paper, we consider the Goldbach＇s problem for matrix rings, namely, we decompose an n × n （n 〉 1） matrix over a principal ideal domain R into a sum of two matrices in Mn,（R） with given determinants. We prove the following result： Let n 〉 1 be a natural number and A = （aij） be a matrix in Mn（R）. Define d（A） ：= g.c.d{aij}. Suppose that p and q are two elements in R. Then （1） If n 〉 1 is even, then A can be written as a sum of two matrices X, Y in Mn（R） with det（X） = p and det（Y） = q if and only if d（A） [ p - q; （2） If n 〉 1 is odd, then A can be written as a sum of two matrices X, Y in Mn（R） with det（X） = p and det（Y） = q if and only if d（A） ｜ p ＋ q. We apply the result to the matrices in Mn（Z） and Mn（Q[x]） and prove that if R = 7. or Q[x], then any nonzero matrix A in Mn（R） can be written as a sum of two matrices in Mn（R） with prime determinants.

Adjustable transmission properties through ring-shaped nanotube arrays using finite-difference time-domain method

Metallic ring-shaped nanotube arrays are proposed and its optical transmission properties are studied by using finite-difference time-domain(FDTD) method.Compared with the transmission spectra of conventional circular nanotube arrays,two photonic band gaps are emerged in the transmission spectra of ring-shaped nanotube arrays,the two band gaps and transmission spectra are adjusted by the length,inner radius,intertube spacing and the dielectric constants of the core and embedding medium,and magnitude modification,redshift and blueshift of the resonance modes are observed.A metallic ring-shaped nanotube arrays for subwavelength band-stop filter in the range of visible light can be achieved.To understand its physical origin,field-interference mechanism was suggested by the field distributions.The proposed nanostructures and results may have great potential applications in subwavelength near-field optics.

面向V2I通信的异构跨域条件隐私保护环签密方案

V2I通信是车载自组织网络的重要组成部分。确保在V2I中消息的安全传输一直是近年来研究人员研究的热点,提出了各种适用于V2I通信的条件隐私保护方案。然而,现有大多数基于环签密的方案中,普遍的假设是发送者和接收者都必须处于相同的密码体制和使用相同系统参数的同一域中。随着通信场景的复杂化,这种假设存在局限性,并且大多数基于环签密的方案未能满足已知临时会话密钥安全。为了解决这些问题,文章提出了一种面向V2I通信的异构跨域条件隐私保护环签密方案。该方案实现了无证书密码体制中的车辆到公钥基础设施密码体制中的基础设施的跨域通信。在随机预言机模型中进行了严格安全性证明,结果显示文章方案满足机密性和不可伪造性。与4种提供类似功能的方案进行比较分析,文章方案具有最低的计算成本和跟踪成本。

RNF122诱导细胞凋亡功能依赖于其RING结构域

人类功能基因RNF122能够明显抑制细胞生长,导致细胞凋亡.RNF122含有RING-H2结构域.为了研究RNF122的RING结构域和凋亡的相互关系,构建了RING结构域突变体.MTT和凋亡实验发现,RNF122与细胞存活的密切关系依赖于其RING结构域.进一步的实验提示,RNF122能够负向调节ERK通路,而RING结构域突变型RNF122则能够增强ERK的磷酸化,提示RNF122可能通过ERK通路调节细胞的存活.总之,RING结构域对于RNF122发挥功能起至关重要的作用.

基于光纤环衰荡技术的锥形光纤磁场测量方法

针对目前光纤磁场传感器解调方法中存在的实用性差且造价昂贵的问题,提出了一种基于光纤环衰荡技术的锥形光纤磁场测量方法,实现了磁场的时间域解调。施加不同强度的磁场时,光在锥形光纤处引起的损耗不同,导致系统衰减时间不同。将系统衰减时间和磁场强度建立联系,实现了磁场测量。为降低传感单元温度和磁场之间的交叉敏感,设计了温度控制电路,将传感单元的温度设定在25.0℃。实验结果表明,该传感器在磁场强度2 mT~9 mT范围内时,磁场强度H和衰减时间τ具有R^(2)=0.98273的线性拟合度,计算得到该传感器的磁场灵敏度为-11.65μs/mT。与光纤磁场传感器的其他解调方式相比,采用光纤环衰荡技术的磁场测量方法,具有响应速度快、成本低、稳定性好的优点。

Bezout domain环上矩阵的广义逆

给出了Bezoutdomain环上矩阵广义逆的定义,并利用Bezoutdomain环酉矩阵的概念得出A+存在的充要条件及(i,…,j)-逆的表达式,从而得到一些有意义的结果。

沙田柚RING-H2 finger基因的生物信息学分析

为探讨沙田柚自交不亲和性的机理,利用高通量测序技术对沙田柚自交和异交花柱进行转录组测序,通过差异分析得到了沙田柚RING-H2 finger基因序列。采用生物信息学方法,对其编码的蛋白质从序列特征、理化性质、跨膜结构域、高级结构以及功能域等方面进行了预测和分析。结果表明:该基因全长为845 bp,开放阅读框(ORF)全长为387 bp,编码128个氨基酸,编码蛋白质的分子量为13.61 KDa,理论等电点为4.26;该蛋白质含有一个植物RING-H2 finger蛋白家族的保守结构域,为疏水性不稳定蛋白。氨基酸序列分析表明,其编码的氨基酸与甜橙(Cs4g15340.1)RING-H2 finger蛋白的相似度为98%。这些分析结果可为今后深入研究该蛋白的结构特征和功能提供参考。

基于TCGA数据库分析UHRF1基因在恶性胸膜间皮瘤中的表达及预后意义

利用生物信息学数据库分析泛素样含PHD和环指结构域蛋白1(UHRF1)在恶性胸膜间皮瘤(MPM)中的表达水平及临床意义。基于TCGA数据库和GTEx数据库差异表达分析UHRF1 m RNA在MPM组织和正常肺组织中的表达水平;使用R软件分析UHRF1 mRNA表达量与临床病理参数的相关性;构建Kaplan-Meier模型和单因素多因素COX回归模型分析UHRF1基因在MPM中的预后;利用TIMER2.0数据库分析UHRF1基因与免疫细胞浸润的关系;GSEA分析UHRF1基因发挥功能的主要富集通路。选取8例MPM组织及4例非MPM胸膜组织,通过RT-q PCR的方法验证UHRF1在MPM与非MPM胸膜组织的表达情况。数据库分析结果表明,与正常肺组织相比,UHRF1 m RNA在MPM组织中高表达;UHRF1高表达患者提示MPM患者预后不良;UHRF1基因表达量与CD4^(+)辅助型T细胞2、CD4^(+)效应记忆性T细胞、巨噬细胞等多种免疫细胞浸润水平具有显著的相关性(P<0.01),且显著影响MPM患者的预后。功能富集分析显示,UHRF1主要在DNA复制、蛋白酶体、同源重组等通路中起作用。在收集到的病例样本中,与非MPM胸膜组织相比,UHRF1 mRNA在MPM组织中的表达显著增高(P<0.001)。UHRF1在MPM组织中高表达,可能通过调节DNA甲基化和免疫细胞浸润来影响MPM患者预后,有望成为MPM治疗和预后评估的潜在靶点。

植物含跨膜域RING E3泛素连接酶研究进展

E3泛素连接酶(ubiquitin ligase enzyme)能识别底物蛋白并将其泛素化,导致底物蛋白通过26S蛋白酶体进行降解,是调节蛋白水平的重要因子。植物E3泛素连接酶在调控激素响应、参与形态建成、抗病防御反应和非生物胁迫响应方面起着重要作用。RING(Really Interesting New Gene 1)家族是含环指结构(RING finger domain)的E3泛素连接酶家族,一般定位于细胞核。含有跨膜结构域(transmembrane domain,TMD)定位于膜的RING E3泛素连接酶(TMD-RING)是该家族中较为特殊的亚家族。通过对植物中含有跨膜域RING E3泛素连接酶的研究进展进行总结,以期为此类基因研究提供借鉴和帮助。

人类RING型锌指蛋白DTX2的全长cDNA克隆与序列分析

鉴定了一个人类WWE与RING型锌指基因DTX2,位于人染色体7q11．23．通过RACE获得的全长cDNA显示,DTX2编码包含两个WWE区域与一个RING锌指区域的蛋白．

Krull整环的Gorenstein同调刻画

从Gorenstein同调代数的角度给出Krull整环新的阐述.应用w-算子,证明整环R是Krull的当且仅当对R的任何非零真w-理想,它w-商环的Gorenstein整体维数都为0.

两芯环形通信物联网中压配网设备管理系统研究

针对中压配网设备管理松散、管理效率低的问题,通过融合物联网技术对管理模式进行优化改进,设计了两芯环形通信物联网中压配网设备管理系统。采用网格化管理模式组成严谨的设备管理方式,利用多种终端提高指令的强控性。设计两芯环形通信线路,避免了传输信号的中断,从而保证管理指令的顺利传达。在技术上,为了提高数据信息处理能力,构建了改进信赖域算法。该算法通过运行状态中的有功和无功功率,有效保证了系统节点传输过程的安全性。通过Proteus软件,仿真配网设备运行过程。试验结果表明,网格化中压配网设备管理系统负荷最大为473.58 kVA,管理指令传输速度为8.4 MB/s,设备管理效率为96.5%。通过仿真对比三种不同管理模式指令传输速度和设备管理效率曲线,验证了所提系统的优越性。

TaGW2L,a GW2-like RING finger E3 ligase,positively regulates heading date in common wheat(Triticum aestivum L.)

RING finger E3 ligases play an important role in regulating plant growth and development by mediating substrate degradation.In this study,we identified TaGW2L,encoding a Grain width and weight2(GW2)-like RING finger E3 ligase,as a novel positive regulator of heading date in wheat(Triticum aestivum L.).TaGW2L exhibited high amino acid sequence similarities with TaGW2 homoeologs,particularly in the conserved RING finger domain.Expression analysis indicated that TaGW2L was constitutively expressed in various wheat tissues.TaGW2L showed transactivation activity in yeast and could interact with the ubiquitin-conjugating enzymes E2_(s).An in vitro ubiquitination assay verified that TaGW2L possessed a similar E3 ligase activity to TaGW2.Overexpression of the TaGW2L-7A homoeolog in wheat led to a significantly earlier heading date under both natural conditions and long-day conditions.Transcriptome analysis revealed that multiple known genes positively regulating wheat heading were significantly upregulated in the TaGW2L-7A-overexpression plants compared with the wild-type control.Together,our findings shed light on the role of TaGW2L in wheat heading date and provide potential applications of TaGW2L for the adaptation improvement of crops.

The Generalization and Proof of “Square Root of 2 Is Not a Rational Number” on the Integral Domain

In this paper, the traditional proof of “square root of 2 is not a rational number” has been reviewed, and then the theory has been generalized to “if n is not a square, square root of n is not a rational number”. And then some conceptions of ring, integral domain, ideal, quotient ring in Advanced algebra, have been introduced. Integers can be regarded as an integral domain, the rational numbers can be regard as a fractional domain. Evens and odds are principal ideals in integral domain. The operations on evens and odds are operations on quotient ring. After introducing “the minimalist form” in fraction ring. The paper proves the main conclusion: in a integral domain, multiplicative subset S produces a fraction ring S−1R, and n is not a square element in R, then to every element a∈R, a2≠n.

若干非圆弧的扇形环域上二重积分变换

本文介绍特殊区域下二重积分变换.

胃癌组织中UHRF1、YAP1和FOXP3表达及与患者临床病理特征的关系

目的探讨胃癌组织中类泛素样含环指域蛋白1(UHRF1)、Yes相关蛋白1(YAP1)和叉头翼状螺旋转录因子3(FOXP3)表达及与患者临床病理特征的关系。方法收集2021年1至12月湖州市第一人民医院经手术切除的85例胃癌患者的癌组织与配对癌旁组织,采用免疫组化法测定UHRF1、YAP1和FOXP3的阳性率。比较胃癌组织与癌旁组织及不同临床病理特征患者间UHRF1、YAP1和FOXP3的阳性率。结果胃癌组织中UHRF1、YAP1和FOXP3阳性率均显著高于癌旁组织,差异均有统计学意义(均P<0.01)。不同性别、年龄、BMI和肿瘤直径胃癌患者UHRF1、YAP1和FOXP3阳性率比较,差异均无统计意义(均P>0.05);低分化胃癌患者高于高、中分化胃癌患者,Ⅲ、Ⅳ期胃癌患者高于Ⅰ、Ⅱ期胃癌患者,淋巴结转移胃癌患者高于无淋巴结转移胃癌患者,差异均有统计学意义(均P<0.05)。结论胃癌组织UHRF1、YAP1和FOXP3阳性率高,并与患者肿瘤分化程度、临床分期和淋巴结转移等临床病理特征有关。

空域无源光纤腔衰荡乙炔体积分数检测仿真

为了更准确地分析空域无源光纤腔衰荡乙炔体积分数检测系统,建立了考虑系统噪声的气体体积分数传感理论模型。采用该模型对空域无源光纤腔衰荡乙炔体积分数检测系统的性能进行了仿真和讨论。结果表明,利用空域无源光纤腔衰荡传感技术,通过测量衰荡距离,可实现灵敏度高达56.226 km^(-1)的乙炔体积分数的测量,稳定性达0.47%,检测极限达260.577×10^(-6),且稳定性和检测极限还可通过减少光纤腔的固有腔损耗得到进一步的提高。此研究对于体积分数传感系统的优化设计应用具有理论指导意义。