Prognostic role of ring finger and WD repeat domain 3 and immune cell infiltration in hepatocellular carcinoma

We have found that the expression of ring finger and WD repeat domain 3(RFWD3)is significantly higher in unpaired and paired hepatocellular carcinoma(HCC)tissues than in normal tissues.Moreover,this expression has a significant correlation with the infiltration level of 14 immune cell types and when the detected RFWD3 expression levels were grouped as high and low,a prominent difference was revealed for overall survival,disease-specific survival,and progression-free interval.Through statistical analysis(univariate Cox),we were also able to identify RFWD3 as an independent prognostic element for HCC,with RFWD3 having an ability to accurately predict HCC prognosis(area under the curve of 0.863).Finally,we have generated prognostic nomograms for probabilities of 1-,3-and 5-year overall survival in HCC via integrating the factors of age,pathologic stage,alpha-fetoprotein level,and RFWD3 expression.

Comparison of the Kobayashi-Royden and Sibony metrics on ring domains

In this paper we show that the Kobayashi-Royden metric and the Sibony metric are different on ring domains,i.e.,the difference of two concentric balls,in higher dimension.

RING finger and WD repeat domain 3 regulates proliferation and metastasis through the Wnt/β-catenin signalling pathways in hepatocellular carcinoma

BACKGROUND Hepatocellular carcinoma(HCC)exhibits high invasiveness and mortality rates,and the molecular mechanisms of HCC have gained increasing research interest.The abnormal DNA damage response has long been recognized as one of the important factors for tumor occurrence and development.Recent studies have shown the potential of the protein RING finger and WD repeat domain 3(RFWD3)that positively regulates p53 stability in response to DNA damage as a therapeutic target in cancers.AIM To investigate the relationship between HCC and RFWD3 in vitro and in vivo and explored the underlying molecular signalling transduction pathways.METHODS RFWD3 gene expression was analyzed in HCC tissues and adjacent normal tissues.Lentivirus was used to stably knockdown RFWD3 expression in HCC cell lines.After verifying the silencing efficiency,Celigo/cell cycle/apoptosis and MTT assays were used to evaluate cell proliferation and apoptosis.Subsequently,cell migration and invasion were assessed by wound healing and transwell assays.In addition,transduced cells were implanted subcutaneously and injected into the tail vein of nude mice to observe tumor growth and metastasis.Next,we used lentiviral-mediated rescue of RFWD3 shRNA to verify the phenotype.Finally,the microarray,ingenuity pathway analysis,and western blot analysis were used to analyze the regulatory network underlying HCC.RESULTS Compared with adjacent tissues,RFWD3 expression levels were significantly higher in clinical HCC tissues and correlated with tumor size and TNM stage(P<0.05),which indicated a poor prognosis state.RFWD3 silencing in BEL-7404 and HCC-LM3 cells increased apoptosis,decreased growth,and inhibited the migration in shRNAi cells compared with those in shCtrl cells(P<0.05).Furthermore,the in vitro results were supported by the findings of the in vivo experiments with the reduction of tumor cell invasion and migration.Moreover,the rescue of RFWD3 shRNAi resulted in the resumption of invasion and metastasis in HCC cell lines.Finally,gene expression profiling and subsequent experimental verification revealed that RFWD3 might influence the proliferation and metastasis of HCC via the Wnt/β-catenin signalling pathway.CONCLUSION We provide evidence for the expression and function of RFWD3 in HCC.RFWD3 affects the prognosis,proliferation,invasion,and metastasis of HCC by regulating the Wnt/β-catenin signalling pathway.

Goldbach's Problem in the Matrix Ring over a Principal Ideal Domain

In this paper, we consider the Goldbach＇s problem for matrix rings, namely, we decompose an n × n （n 〉 1） matrix over a principal ideal domain R into a sum of two matrices in Mn,（R） with given determinants. We prove the following result： Let n 〉 1 be a natural number and A = （aij） be a matrix in Mn（R）. Define d（A） ：= g.c.d{aij}. Suppose that p and q are two elements in R. Then （1） If n 〉 1 is even, then A can be written as a sum of two matrices X, Y in Mn（R） with det（X） = p and det（Y） = q if and only if d（A） [ p - q; （2） If n 〉 1 is odd, then A can be written as a sum of two matrices X, Y in Mn（R） with det（X） = p and det（Y） = q if and only if d（A） ｜ p ＋ q. We apply the result to the matrices in Mn（Z） and Mn（Q[x]） and prove that if R = 7. or Q[x], then any nonzero matrix A in Mn（R） can be written as a sum of two matrices in Mn（R） with prime determinants.

面向V2I通信的异构跨域条件隐私保护环签密方案

V2I通信是车载自组织网络的重要组成部分。确保在V2I中消息的安全传输一直是近年来研究人员研究的热点,提出了各种适用于V2I通信的条件隐私保护方案。然而,现有大多数基于环签密的方案中,普遍的假设是发送者和接收者都必须处于相同的密码体制和使用相同系统参数的同一域中。随着通信场景的复杂化,这种假设存在局限性,并且大多数基于环签密的方案未能满足已知临时会话密钥安全。为了解决这些问题,文章提出了一种面向V2I通信的异构跨域条件隐私保护环签密方案。该方案实现了无证书密码体制中的车辆到公钥基础设施密码体制中的基础设施的跨域通信。在随机预言机模型中进行了严格安全性证明,结果显示文章方案满足机密性和不可伪造性。与4种提供类似功能的方案进行比较分析,文章方案具有最低的计算成本和跟踪成本。

非肌层浸润性膀胱癌组织中OTUD5和RNF186表达与临床病理特征的关系及预后价值研究

目的研究非肌层浸润膀胱癌(non-muscle invasive bladder cancer,NMIBC)癌组织中结构域蛋白去泛素化酶5(domain protein ubiquitin 5,OTUD5)、环指蛋白186(ring finger protein 186,RNF186)表达与临床病理特征的关系及预后价值研究。方法收集2019年1月~2020年1月唐山市中医院收治的106例NMIBC患者。免疫组织化学检测NMIBC癌组织及癌旁组织OTUD5,RNF186表达。比较不同临床病理特征NMIBC癌组织OTUD5,RNF186表达差异。Kaplan-Meier曲线分析(Log-Rank检验)OTUD5,RNF186表达对NMIBC无进展生存预后的影响。COX回归模型分析影响NMIBC患者无进展生存预后的因素。结果NMIBC癌组织中OTUD5(62.26%),RNF186(66.04%)的阳性率高于癌旁组织(11.32%,8.49%),差异具有统计学意义(χ^(2)=59.146,75.079,均P<0.05)。NMIBC癌组织中OTUD5与RNF186表达呈显著正相关(r=0.659,P<0.05)。T 1期、高级别尿路上皮癌NMIBC癌组织中OTUD5(72.97%,84.21%),RNF186(77.03,86.84%)阳性率高于Ta/Tis期(37.50%,50.00%)、低级别尿路上皮癌(40.63%,54.41%),差异具有统计学意义(χ^(2)=11.964,13.199;12.143,11.431,均P<0.05)。OTUD5阳性组(40.91%)、RNF186阳性组(45.71%)三年累积无进展生存率低于OTUD5阴性组(90.00%)、RNF186阴性组(86.11%)患者(Log-Ranktestχ^(2)=24.710,14.586,均P<0.05)。OTUD5阳性(OR=1.446,95%CI:1.086~1.925)、RNF186阳性(OR=1.579,95%CI:1.132~2.024)、肿瘤TNM分期T1期(OR=1.582,95%CI:1.219~2.054)、病理分级高级别(OR=1.570,95%CI:1.188~2.074)是影响NMIBC患者无进展生存的独立危险因素(均P<0.001)。结论NMIBC患者癌组织OTUD5,RNF186表达升高,与肿瘤TNM分期、病理分级有关,是影响NMIBC患者预后的独立危险因素。

食管鳞癌组织中BARD1的表达及与淋巴结转移的关系

目的分析食管鳞癌(ESCC)中乳腺癌易感基因1相关环指结构域蛋白1(BARD1)的表达及与淋巴结转移的关系。方法纳入2018年1月至2021年12月本院收治且经组织病理学检查确诊的114例ESCC患者,免疫组化EnVision两步法检测癌组织与癌旁组织中BARD1的表达情况并比较BARD1阳性率,分析BARD1表达与ESCC临床病理特征的关系,建立Logistic回归模型分析BARD1表达对ESCC淋巴结转移的影响,绘制受试者工作特征(ROC)曲线分析BARD1表达对ES⁃CC淋巴结转移的预测价值。结果114例ESCC组织中BARD1阳性78例、阴性36例,对应癌旁组织中BARD1阳性23例、阴性91例,ESCC组织的BARD1阳性率为68.42%,高于癌旁组织的20.18%(χ^(2)=53.769,P<0.001)。BARD1表达与ESCC患者的T分期、分化程度和淋巴结转移有关(P<0.05),其中淋巴结转移组织的BARD1阳性率为80.36%(45/56),高于未转移组织的56.90%(33/58)。经Phi系数分析发现,BARD1表达与ESCC淋巴结转移呈正相关(r=0.276,P=0.003)。BARD1阳性是ESCC患者淋巴结转移的独立危险因素(OR=3.099,95%CI:1.339~7.175,P=0.008),且BARD1表达对ESCC患者淋巴结转移具有一定预测价值(AUC=0.636,P=0.020)。结论BARD1在ESCC中高表达,其与ESCC淋巴结转移密切相关,BARD1阳性会增加淋巴结转移风险,在评估ESCC淋巴结转移上有一定价值。

RNF122诱导细胞凋亡功能依赖于其RING结构域

人类功能基因RNF122能够明显抑制细胞生长,导致细胞凋亡.RNF122含有RING-H2结构域.为了研究RNF122的RING结构域和凋亡的相互关系,构建了RING结构域突变体.MTT和凋亡实验发现,RNF122与细胞存活的密切关系依赖于其RING结构域.进一步的实验提示,RNF122能够负向调节ERK通路,而RING结构域突变型RNF122则能够增强ERK的磷酸化,提示RNF122可能通过ERK通路调节细胞的存活.总之,RING结构域对于RNF122发挥功能起至关重要的作用.

基于光纤环衰荡技术的锥形光纤磁场测量方法

针对目前光纤磁场传感器解调方法中存在的实用性差且造价昂贵的问题,提出了一种基于光纤环衰荡技术的锥形光纤磁场测量方法,实现了磁场的时间域解调。施加不同强度的磁场时,光在锥形光纤处引起的损耗不同,导致系统衰减时间不同。将系统衰减时间和磁场强度建立联系,实现了磁场测量。为降低传感单元温度和磁场之间的交叉敏感,设计了温度控制电路,将传感单元的温度设定在25.0℃。实验结果表明,该传感器在磁场强度2 mT~9 mT范围内时,磁场强度H和衰减时间τ具有R^(2)=0.98273的线性拟合度,计算得到该传感器的磁场灵敏度为-11.65μs/mT。与光纤磁场传感器的其他解调方式相比,采用光纤环衰荡技术的磁场测量方法,具有响应速度快、成本低、稳定性好的优点。

Bezout domain环上矩阵的广义逆

给出了Bezoutdomain环上矩阵广义逆的定义,并利用Bezoutdomain环酉矩阵的概念得出A+存在的充要条件及(i,…,j)-逆的表达式,从而得到一些有意义的结果。

植物含跨膜域RING E3泛素连接酶研究进展

E3泛素连接酶(ubiquitin ligase enzyme)能识别底物蛋白并将其泛素化,导致底物蛋白通过26S蛋白酶体进行降解,是调节蛋白水平的重要因子。植物E3泛素连接酶在调控激素响应、参与形态建成、抗病防御反应和非生物胁迫响应方面起着重要作用。RING(Really Interesting New Gene 1)家族是含环指结构(RING finger domain)的E3泛素连接酶家族,一般定位于细胞核。含有跨膜结构域(transmembrane domain,TMD)定位于膜的RING E3泛素连接酶(TMD-RING)是该家族中较为特殊的亚家族。通过对植物中含有跨膜域RING E3泛素连接酶的研究进展进行总结,以期为此类基因研究提供借鉴和帮助。

沙田柚RING-H2 finger基因的生物信息学分析

为探讨沙田柚自交不亲和性的机理,利用高通量测序技术对沙田柚自交和异交花柱进行转录组测序,通过差异分析得到了沙田柚RING-H2 finger基因序列。采用生物信息学方法,对其编码的蛋白质从序列特征、理化性质、跨膜结构域、高级结构以及功能域等方面进行了预测和分析。结果表明:该基因全长为845 bp,开放阅读框(ORF)全长为387 bp,编码128个氨基酸,编码蛋白质的分子量为13.61 KDa,理论等电点为4.26;该蛋白质含有一个植物RING-H2 finger蛋白家族的保守结构域,为疏水性不稳定蛋白。氨基酸序列分析表明,其编码的氨基酸与甜橙(Cs4g15340.1)RING-H2 finger蛋白的相似度为98%。这些分析结果可为今后深入研究该蛋白的结构特征和功能提供参考。

基于TCGA数据库分析UHRF1基因在恶性胸膜间皮瘤中的表达及预后意义

利用生物信息学数据库分析泛素样含PHD和环指结构域蛋白1(UHRF1)在恶性胸膜间皮瘤(MPM)中的表达水平及临床意义。基于TCGA数据库和GTEx数据库差异表达分析UHRF1 m RNA在MPM组织和正常肺组织中的表达水平;使用R软件分析UHRF1 mRNA表达量与临床病理参数的相关性;构建Kaplan-Meier模型和单因素多因素COX回归模型分析UHRF1基因在MPM中的预后;利用TIMER2.0数据库分析UHRF1基因与免疫细胞浸润的关系;GSEA分析UHRF1基因发挥功能的主要富集通路。选取8例MPM组织及4例非MPM胸膜组织,通过RT-q PCR的方法验证UHRF1在MPM与非MPM胸膜组织的表达情况。数据库分析结果表明,与正常肺组织相比,UHRF1 m RNA在MPM组织中高表达;UHRF1高表达患者提示MPM患者预后不良;UHRF1基因表达量与CD4^(+)辅助型T细胞2、CD4^(+)效应记忆性T细胞、巨噬细胞等多种免疫细胞浸润水平具有显著的相关性(P<0.01),且显著影响MPM患者的预后。功能富集分析显示,UHRF1主要在DNA复制、蛋白酶体、同源重组等通路中起作用。在收集到的病例样本中,与非MPM胸膜组织相比,UHRF1 mRNA在MPM组织中的表达显著增高(P<0.001)。UHRF1在MPM组织中高表达,可能通过调节DNA甲基化和免疫细胞浸润来影响MPM患者预后,有望成为MPM治疗和预后评估的潜在靶点。

人类RING型锌指蛋白DTX2的全长cDNA克隆与序列分析

鉴定了一个人类WWE与RING型锌指基因DTX2,位于人染色体7q11．23．通过RACE获得的全长cDNA显示,DTX2编码包含两个WWE区域与一个RING锌指区域的蛋白．

Krull整环的Gorenstein同调刻画

从Gorenstein同调代数的角度给出Krull整环新的阐述.应用w-算子,证明整环R是Krull的当且仅当对R的任何非零真w-理想,它w-商环的Gorenstein整体维数都为0.

两芯环形通信物联网中压配网设备管理系统研究

针对中压配网设备管理松散、管理效率低的问题,通过融合物联网技术对管理模式进行优化改进,设计了两芯环形通信物联网中压配网设备管理系统。采用网格化管理模式组成严谨的设备管理方式,利用多种终端提高指令的强控性。设计两芯环形通信线路,避免了传输信号的中断,从而保证管理指令的顺利传达。在技术上,为了提高数据信息处理能力,构建了改进信赖域算法。该算法通过运行状态中的有功和无功功率,有效保证了系统节点传输过程的安全性。通过Proteus软件,仿真配网设备运行过程。试验结果表明,网格化中压配网设备管理系统负荷最大为473.58 kVA,管理指令传输速度为8.4 MB/s,设备管理效率为96.5%。通过仿真对比三种不同管理模式指令传输速度和设备管理效率曲线,验证了所提系统的优越性。

TaGW2L,a GW2-like RING finger E3 ligase,positively regulates heading date in common wheat(Triticum aestivum L.)

RING finger E3 ligases play an important role in regulating plant growth and development by mediating substrate degradation.In this study,we identified TaGW2L,encoding a Grain width and weight2(GW2)-like RING finger E3 ligase,as a novel positive regulator of heading date in wheat(Triticum aestivum L.).TaGW2L exhibited high amino acid sequence similarities with TaGW2 homoeologs,particularly in the conserved RING finger domain.Expression analysis indicated that TaGW2L was constitutively expressed in various wheat tissues.TaGW2L showed transactivation activity in yeast and could interact with the ubiquitin-conjugating enzymes E2_(s).An in vitro ubiquitination assay verified that TaGW2L possessed a similar E3 ligase activity to TaGW2.Overexpression of the TaGW2L-7A homoeolog in wheat led to a significantly earlier heading date under both natural conditions and long-day conditions.Transcriptome analysis revealed that multiple known genes positively regulating wheat heading were significantly upregulated in the TaGW2L-7A-overexpression plants compared with the wild-type control.Together,our findings shed light on the role of TaGW2L in wheat heading date and provide potential applications of TaGW2L for the adaptation improvement of crops.

The Effect of a Superstrate Layer on the Input Impedance of An Annular Ring Microstrip Antenna

The method of moments is used to analyze the effect of a superstrate on the input impedance of an annular ring microstrip antenna. Surface current and vertical unit current ae used to model the microstrip patch and the coaxical probe, respectively. The integral equations for the unknown current on the patch are solved by using Galerkins method applied in the Hankel transform domain. The expression for the input impedance is derived according to the patch current. The numerical results for the input impedance are presented for different values of the thickness and relative permittivity of a superstrate layer. It is shown from the numerical results that the superstrate has an effect of decreasing the resonant frequency and changing the input impedance level.

The Generalization and Proof of “Square Root of 2 Is Not a Rational Number” on the Integral Domain

In this paper, the traditional proof of “square root of 2 is not a rational number” has been reviewed, and then the theory has been generalized to “if n is not a square, square root of n is not a rational number”. And then some conceptions of ring, integral domain, ideal, quotient ring in Advanced algebra, have been introduced. Integers can be regarded as an integral domain, the rational numbers can be regard as a fractional domain. Evens and odds are principal ideals in integral domain. The operations on evens and odds are operations on quotient ring. After introducing “the minimalist form” in fraction ring. The paper proves the main conclusion: in a integral domain, multiplicative subset S produces a fraction ring S−1R, and n is not a square element in R, then to every element a∈R, a2≠n.

若干非圆弧的扇形环域上二重积分变换

本文介绍特殊区域下二重积分变换.