Objective： The aim of this study was to investigate the MRP-1/CD9mRNA expression in lung cancer and normal lung tissues and the relationship between its expression and pathologic grades, clinical stages, metastasis a...Objective： The aim of this study was to investigate the MRP-1/CD9mRNA expression in lung cancer and normal lung tissues and the relationship between its expression and pathologic grades, clinical stages, metastasis and prognosis. Methods： To observe MRP-1/C9mRNA expression, tissue microarray （TMA） containing 54 lung cancers and 10 normal lung tissues was prepared and Fluorescence in situ hybridization was used. Results： The positive rate of MRP-1/CD9 expression was 48.1% in lung cancer, lower than that of normal lung tissues. The statistical difference was significant （P〈0.05）. Its protein expression had no relationship with the patients＇ ages, sex and the macroscopic type of tumor, but had relationships with the histological type, clinical stage, differentiated degree and metastasis. The expression in non-small cell lung cancer （NSCLC） was higher than that in small cell lung cancer （SCLC）; in well-moderately differentiated group was higher than that in poorly differentiated group; Earlier period group （I＋II） was higher than in later period group （Ⅲ＋Ⅳ）; and in group without lymphoid metastasis was higher than in patients with lymphoid metastasis. Conclusion： The progression of the lung cancer maybe related with the descended MRP-1/Cd9 expression, which may be useful in evaluating the prognosis of cancer patients.展开更多
Objective To investigate the expression of Survivin mRNA in lung cancer tissue microarray （TMA） by fluorescence in .situ hybridization （FISH） method, and determine the role and significance of it in lung cancer ge...Objective To investigate the expression of Survivin mRNA in lung cancer tissue microarray （TMA） by fluorescence in .situ hybridization （FISH） method, and determine the role and significance of it in lung cancer genesis and progress. Methods The expression of Survivin mRNA was detected by FISH method and TMA technology. Fifty-four cases of lung cancer and 10 cases of normal lung tissue were examined. Survivin mRNA was expressed in 66.7% （36/54） of lung cancer; the positive ratio of lung cancer was significantly higher than that of normal lung tissue （0/10;X^2= 15.238, P 〈 0.05）. The positive ratio of Survivin mRNA was significantly higher in poor differentiated cancer （20/24, 83.3% ） than moderate and well differentiated cancer （16/30, 53.3%; X^2 = 5.40, P 〈 0.05）. The positive ratio of Survivin mRNA was significantly higher in group with lymph node metastasis （27/32, 84.4%） than without lymph node metastasis （9/22, 40.9%; X^2= 11.084, P 〈 0.05）. The positive ratio of Survivin mRNA was significantly higher in stage Ⅲ-Ⅳ（12/13, 92.3%） than stage Ⅰ- Ⅱ （24/41,58.5%; X^2=5.066, P〈 0.05）. Conclusion Survivin mRNA highly expresses in lung cancer, which is related to the progress and malignant behavior. Survivin may play a promoting role in lung cancer genesis and progress and provide a basis for estimating prognosis and treatment.展开更多
A series of human tissue samples and cultured cell lines were formalin-fixed and paraffin-embedded. Specimen cylinder （1.2 -1.8ram） were punched by a modified bone marrow biopsy needle and arrayed on a recipient par...A series of human tissue samples and cultured cell lines were formalin-fixed and paraffin-embedded. Specimen cylinder （1.2 -1.8ram） were punched by a modified bone marrow biopsy needle and arrayed on a recipient paraffin block. Microscopic analysis on the sections from this tissue microarray （ TMA ） block demonstrated that the spots of tissues and cells were well preserved, and the cultured cell samples were successfully embedded from 5 × 104 to 2 × 105 in number. These TMA sections were also suitable for immunohistochemistry and RNA in situ hybridization.展开更多
AIM:To rapidly detect molecular alterations in different malignancies and investigate the possible role of Tp53,C-myc,and CCND1 genes in development of tumors in human organs and their adjacent normal tissues,as well ...AIM:To rapidly detect molecular alterations in different malignancies and investigate the possible role of Tp53,C-myc,and CCND1 genes in development of tumors in human organs and their adjacent normal tissues,as well as the possible relation between well-and poorly-differentiated tumors.METHODS:A tissue array consisting of seven different tumors was generated.The tissue array included 120 points of esophagus,120 points of stomach,80 points of rectum,60 points of thyroid gland,100 points of mammary gland,80 points of liver,and 80 points of colon.Expressions of Tp53,C-myc,and CCND1 were determined by RNA in situ hybridization.3' terminal digoxin-labeled anti-sense single stranded oligonucleotide and locked nucleic acid modifying probe were used.RESULTS:The expression level of Tp53 gene was higher in six different carcinoma tissue samples than in paracancerous tissue samples with the exception in colon carcinoma tissue samples(P < 0.05).The expression level of CCND1 gene was significantly different in different carcinoma tissue samples with the exception in esophagus and colon carcinoma tissue samples.The expression level of C-myc gene was different in esophagus carcinoma tissue samples(c2 = 18.495,P = 0.000),stomach carcinoma tissue samples(c2 = 23.750,P = 0.000),and thyroid gland tissue samples(c2 = 10.999,P = 0.004).The intensity of signals was also different in different carcinoma tissue samples and paracancerous tissue samples.CONCLUSION:Over-expression of the Tp53,CCND1,and C-myc genes appears to play a role in development of human cancer by regulating the expression of mRNA.Tp53,CCND1 and C-myc genes are significantly correlated with the development of different carcinomas.展开更多
OBJECTIVE Numerous microRNAs (miRNAs) are deregulatedin human cancers. The experimental evidence supports thatmiRNAs plays a role in the initiation and progression of humanmalignancies.The present study was undertaken...OBJECTIVE Numerous microRNAs (miRNAs) are deregulatedin human cancers. The experimental evidence supports thatmiRNAs plays a role in the initiation and progression of humanmalignancies.The present study was undertaken to evaluatethe differential expression of 6 miRNAs as biomarker for earlydetection of prostate cancer, and then to determine whether theexpression profiling of these miRNAs could predict the prognosisof prostate cancer.METHODS The expression profilings of these 6 miRNAs wereinvestigated using the method of locked nucleic acid (LNA)-modified oligonucleotide in situ hybridization (ISH). And thetechnology of tissue microarray (TMA) was employed using theformalin-fixed, paraffin-embedd (FFPE) specimens taken from52 patients with prostate carcinoma (PCa) and 38 patients withbenign prostatic hyperplasia (BPH).RESULTS The rates of positive expression for 6 miRNAs (miR-15b, miR-16, let-7g, miR- 96,miR-182 and miR-183) were 26.92%,15.38%, 15.38%, 67.31%, 61.54% and 71.15% in the specimens ofprostate cancer, and 57.89%, 76.32%, 68.42%, 44.74%, 31.58%,47.37% in the tissues of benign prostatic hyperplasia, respectively.The expressions of all 6 miRNAs between the prostate cancer andbenign prostatic hyperplasia tissues were significantly different(P < 0.05). The positive rate of these 6 miRNAs was significantlyrelated to the Gleason Grading of prostate cancer (P < 0.01). Therewas no significant correlation between the expression of thesemiRNAs and age and the concentration of serum PSA of thepatient (P >0.05). We also found that the expression of miR-15b,miR-96 and miR-182 correlated with clinical stages of tumor (P <0.05). The expression of miR-96 correlated with lobus prostatae oftumor invasion (P < 0.01), but the expressions of the remaining fivemiRNAs were not correlated with that (P >0.05). In addition, theexpression of miR-15b was negatively related to that of miR-96,miR-182 and miR-183, respectively (P < 0.01, r < 0.00).There wasa positive correlation among the expressions of miR-96, miR-182and miR-183 in prostate cancer (P < 0.01, r >0.00). The expressionof miR-16 was positively related to that of miR-let-7g (P < 0.01, r >0.00).CONCLUSION The results suggest that miRNA expressionprofiling could have relevance to the biological and clinicalbehavior of prostate cancer,and they might be importantbiomarkers for early detection and prognostic assessment ofprostate cancer.展开更多
基金This work was supported by a grant from Tianjin Science and Technology Committee (No. 033804211)
文摘Objective： The aim of this study was to investigate the MRP-1/CD9mRNA expression in lung cancer and normal lung tissues and the relationship between its expression and pathologic grades, clinical stages, metastasis and prognosis. Methods： To observe MRP-1/C9mRNA expression, tissue microarray （TMA） containing 54 lung cancers and 10 normal lung tissues was prepared and Fluorescence in situ hybridization was used. Results： The positive rate of MRP-1/CD9 expression was 48.1% in lung cancer, lower than that of normal lung tissues. The statistical difference was significant （P〈0.05）. Its protein expression had no relationship with the patients＇ ages, sex and the macroscopic type of tumor, but had relationships with the histological type, clinical stage, differentiated degree and metastasis. The expression in non-small cell lung cancer （NSCLC） was higher than that in small cell lung cancer （SCLC）; in well-moderately differentiated group was higher than that in poorly differentiated group; Earlier period group （I＋II） was higher than in later period group （Ⅲ＋Ⅳ）; and in group without lymphoid metastasis was higher than in patients with lymphoid metastasis. Conclusion： The progression of the lung cancer maybe related with the descended MRP-1/Cd9 expression, which may be useful in evaluating the prognosis of cancer patients.
基金Supported byaK eyProjectofTianjin ScientificCom m ittee(033804211).
文摘Objective To investigate the expression of Survivin mRNA in lung cancer tissue microarray （TMA） by fluorescence in .situ hybridization （FISH） method, and determine the role and significance of it in lung cancer genesis and progress. Methods The expression of Survivin mRNA was detected by FISH method and TMA technology. Fifty-four cases of lung cancer and 10 cases of normal lung tissue were examined. Survivin mRNA was expressed in 66.7% （36/54） of lung cancer; the positive ratio of lung cancer was significantly higher than that of normal lung tissue （0/10;X^2= 15.238, P 〈 0.05）. The positive ratio of Survivin mRNA was significantly higher in poor differentiated cancer （20/24, 83.3% ） than moderate and well differentiated cancer （16/30, 53.3%; X^2 = 5.40, P 〈 0.05）. The positive ratio of Survivin mRNA was significantly higher in group with lymph node metastasis （27/32, 84.4%） than without lymph node metastasis （9/22, 40.9%; X^2= 11.084, P 〈 0.05）. The positive ratio of Survivin mRNA was significantly higher in stage Ⅲ-Ⅳ（12/13, 92.3%） than stage Ⅰ- Ⅱ （24/41,58.5%; X^2=5.066, P〈 0.05）. Conclusion Survivin mRNA highly expresses in lung cancer, which is related to the progress and malignant behavior. Survivin may play a promoting role in lung cancer genesis and progress and provide a basis for estimating prognosis and treatment.
文摘A series of human tissue samples and cultured cell lines were formalin-fixed and paraffin-embedded. Specimen cylinder （1.2 -1.8ram） were punched by a modified bone marrow biopsy needle and arrayed on a recipient paraffin block. Microscopic analysis on the sections from this tissue microarray （ TMA ） block demonstrated that the spots of tissues and cells were well preserved, and the cultured cell samples were successfully embedded from 5 × 104 to 2 × 105 in number. These TMA sections were also suitable for immunohistochemistry and RNA in situ hybridization.
基金Supported by Foundation of Xiamen Science and Technology Bureau,Fujian Province,China,No.3502Z20074023
文摘AIM:To rapidly detect molecular alterations in different malignancies and investigate the possible role of Tp53,C-myc,and CCND1 genes in development of tumors in human organs and their adjacent normal tissues,as well as the possible relation between well-and poorly-differentiated tumors.METHODS:A tissue array consisting of seven different tumors was generated.The tissue array included 120 points of esophagus,120 points of stomach,80 points of rectum,60 points of thyroid gland,100 points of mammary gland,80 points of liver,and 80 points of colon.Expressions of Tp53,C-myc,and CCND1 were determined by RNA in situ hybridization.3' terminal digoxin-labeled anti-sense single stranded oligonucleotide and locked nucleic acid modifying probe were used.RESULTS:The expression level of Tp53 gene was higher in six different carcinoma tissue samples than in paracancerous tissue samples with the exception in colon carcinoma tissue samples(P < 0.05).The expression level of CCND1 gene was significantly different in different carcinoma tissue samples with the exception in esophagus and colon carcinoma tissue samples.The expression level of C-myc gene was different in esophagus carcinoma tissue samples(c2 = 18.495,P = 0.000),stomach carcinoma tissue samples(c2 = 23.750,P = 0.000),and thyroid gland tissue samples(c2 = 10.999,P = 0.004).The intensity of signals was also different in different carcinoma tissue samples and paracancerous tissue samples.CONCLUSION:Over-expression of the Tp53,CCND1,and C-myc genes appears to play a role in development of human cancer by regulating the expression of mRNA.Tp53,CCND1 and C-myc genes are significantly correlated with the development of different carcinomas.
文摘OBJECTIVE Numerous microRNAs (miRNAs) are deregulatedin human cancers. The experimental evidence supports thatmiRNAs plays a role in the initiation and progression of humanmalignancies.The present study was undertaken to evaluatethe differential expression of 6 miRNAs as biomarker for earlydetection of prostate cancer, and then to determine whether theexpression profiling of these miRNAs could predict the prognosisof prostate cancer.METHODS The expression profilings of these 6 miRNAs wereinvestigated using the method of locked nucleic acid (LNA)-modified oligonucleotide in situ hybridization (ISH). And thetechnology of tissue microarray (TMA) was employed using theformalin-fixed, paraffin-embedd (FFPE) specimens taken from52 patients with prostate carcinoma (PCa) and 38 patients withbenign prostatic hyperplasia (BPH).RESULTS The rates of positive expression for 6 miRNAs (miR-15b, miR-16, let-7g, miR- 96,miR-182 and miR-183) were 26.92%,15.38%, 15.38%, 67.31%, 61.54% and 71.15% in the specimens ofprostate cancer, and 57.89%, 76.32%, 68.42%, 44.74%, 31.58%,47.37% in the tissues of benign prostatic hyperplasia, respectively.The expressions of all 6 miRNAs between the prostate cancer andbenign prostatic hyperplasia tissues were significantly different(P < 0.05). The positive rate of these 6 miRNAs was significantlyrelated to the Gleason Grading of prostate cancer (P < 0.01). Therewas no significant correlation between the expression of thesemiRNAs and age and the concentration of serum PSA of thepatient (P >0.05). We also found that the expression of miR-15b,miR-96 and miR-182 correlated with clinical stages of tumor (P <0.05). The expression of miR-96 correlated with lobus prostatae oftumor invasion (P < 0.01), but the expressions of the remaining fivemiRNAs were not correlated with that (P >0.05). In addition, theexpression of miR-15b was negatively related to that of miR-96,miR-182 and miR-183, respectively (P < 0.01, r < 0.00).There wasa positive correlation among the expressions of miR-96, miR-182and miR-183 in prostate cancer (P < 0.01, r >0.00). The expressionof miR-16 was positively related to that of miR-let-7g (P < 0.01, r >0.00).CONCLUSION The results suggest that miRNA expressionprofiling could have relevance to the biological and clinicalbehavior of prostate cancer,and they might be importantbiomarkers for early detection and prognostic assessment ofprostate cancer.
