目的:观察甲氨蝶呤(MTX)联合不同中药影响Ⅱ型胶原诱导关节炎(CIA)大鼠血清肿瘤坏死因子-α(TNF-α)以及孤核受体m RNA(RORγt m RNA)表达的差异。方法:将Waster大鼠随机分为5组,建立Ⅱ型胶原诱导关节炎模型,正常组和模型组给予0.9%氯...目的:观察甲氨蝶呤(MTX)联合不同中药影响Ⅱ型胶原诱导关节炎(CIA)大鼠血清肿瘤坏死因子-α(TNF-α)以及孤核受体m RNA(RORγt m RNA)表达的差异。方法:将Waster大鼠随机分为5组,建立Ⅱ型胶原诱导关节炎模型,正常组和模型组给予0.9%氯化钠溶液灌胃,各给药组分别给予对应药品,6周后,各组大鼠经腹主动脉取血,采用ELISA法检测大鼠中血清TNF-α,以荧光PCR法检测大鼠脾脏RORγt m RNA表达,评价MTX联合中药对类风湿性关节炎治疗差异。结果:与正常组比较,模型组大鼠血清TNF-α及脾脏RORγt m RNA表达水平均有所升高(P<0.01);与模型组比较,各治疗组大鼠血清TNF-α及脾脏RORγt m RNA表达水平降低(P<0.01)。与MTX组比较,MTX+雷公藤组和MTX+海蛇药酒组大鼠血清TNF-α及脾脏RORγt m RNA表达水平均明显降低(P<0.05)。结论:MTX、MTX联合雷公藤与海蛇药酒对CIA大鼠有一定的治疗作用,且MTX+雷公藤、MTX+海蛇药酒治疗效果最佳,能显著下调关节滑膜RORγt m RNA的表达,降低血清TNF-α水平,从而发挥抑制关节炎性反应的作用。展开更多
目的观察中药白疕合剂对体外培养人永生化表皮角质形成细胞(Ha Ca T细胞)分泌肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6及TNF-αm RNA、IL-6 m RNA表达的影响,并探讨其作用机制。方法采用ELISA法检测白疕合剂对Ha Ca T细胞分泌TNF-α、I...目的观察中药白疕合剂对体外培养人永生化表皮角质形成细胞(Ha Ca T细胞)分泌肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6及TNF-αm RNA、IL-6 m RNA表达的影响,并探讨其作用机制。方法采用ELISA法检测白疕合剂对Ha Ca T细胞分泌TNF-α、IL-6的影响;采用RT-PCR方法检测白疕合剂对Ha Ca T细胞TNF-αm RNA、IL-6m RNA表达的影响。结果白疕合剂低、中、高剂量组干预Ha Ca T细胞后,对TNF-α、IL-6的分泌量及TNF-αm RNA、IL-6 m RNA的表达均有明显的抑制作用。白疕合剂高剂量组对IL-6分泌量的抑制作用及降低TNF-αm RNA、IL-6m RNA的表达与消银颗粒组和阿维A组比较差异无统计学意义;对TNF-α的抑制作用与消银颗粒组比较差异有统计学意义,与阿维A组比较差异无统计学意义。结论白疕合剂可能通过抑制TNF-α、IL-6的分泌和降低TNF-αm RNA、IL-6 m RNA的表达,发挥对银屑病的治疗作用。展开更多
RNA modifications are abundant in eukaryotes, bacteria, and archaea. N^6-methyladenosine(m^6A), a type of RNA modification mainly found in messenger RNA(mRNA), has significant effects on the metabolism and function of...RNA modifications are abundant in eukaryotes, bacteria, and archaea. N^6-methyladenosine(m^6A), a type of RNA modification mainly found in messenger RNA(mRNA), has significant effects on the metabolism and function of m RNAs. This modification is governed by three types of proteins, namely methyltransferases as ‘‘writers' ', demethylases as ‘‘erasers' ',and specific m^6A-binding proteins(YTHDF1-3) as ‘‘readers' '. Further, it is important for the regulation of cell fate and has a critical function in many biological processes including virus replication, stem cell differentiation, and cancer development, and exerts its effect by controlling gene expression. Herein, we summarize recent advances in research on m^6A in virus replication and T cell regulation, which is a rapidly emerging field that will facilitate the development of antiviral therapies and the study of innate immunity.展开更多
文摘目的:观察甲氨蝶呤(MTX)联合不同中药影响Ⅱ型胶原诱导关节炎(CIA)大鼠血清肿瘤坏死因子-α(TNF-α)以及孤核受体m RNA(RORγt m RNA)表达的差异。方法:将Waster大鼠随机分为5组,建立Ⅱ型胶原诱导关节炎模型,正常组和模型组给予0.9%氯化钠溶液灌胃,各给药组分别给予对应药品,6周后,各组大鼠经腹主动脉取血,采用ELISA法检测大鼠中血清TNF-α,以荧光PCR法检测大鼠脾脏RORγt m RNA表达,评价MTX联合中药对类风湿性关节炎治疗差异。结果:与正常组比较,模型组大鼠血清TNF-α及脾脏RORγt m RNA表达水平均有所升高(P<0.01);与模型组比较,各治疗组大鼠血清TNF-α及脾脏RORγt m RNA表达水平降低(P<0.01)。与MTX组比较,MTX+雷公藤组和MTX+海蛇药酒组大鼠血清TNF-α及脾脏RORγt m RNA表达水平均明显降低(P<0.05)。结论:MTX、MTX联合雷公藤与海蛇药酒对CIA大鼠有一定的治疗作用,且MTX+雷公藤、MTX+海蛇药酒治疗效果最佳,能显著下调关节滑膜RORγt m RNA的表达,降低血清TNF-α水平,从而发挥抑制关节炎性反应的作用。
文摘目的观察中药白疕合剂对体外培养人永生化表皮角质形成细胞(Ha Ca T细胞)分泌肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6及TNF-αm RNA、IL-6 m RNA表达的影响,并探讨其作用机制。方法采用ELISA法检测白疕合剂对Ha Ca T细胞分泌TNF-α、IL-6的影响;采用RT-PCR方法检测白疕合剂对Ha Ca T细胞TNF-αm RNA、IL-6m RNA表达的影响。结果白疕合剂低、中、高剂量组干预Ha Ca T细胞后,对TNF-α、IL-6的分泌量及TNF-αm RNA、IL-6 m RNA的表达均有明显的抑制作用。白疕合剂高剂量组对IL-6分泌量的抑制作用及降低TNF-αm RNA、IL-6m RNA的表达与消银颗粒组和阿维A组比较差异无统计学意义;对TNF-α的抑制作用与消银颗粒组比较差异有统计学意义,与阿维A组比较差异无统计学意义。结论白疕合剂可能通过抑制TNF-α、IL-6的分泌和降低TNF-αm RNA、IL-6 m RNA的表达,发挥对银屑病的治疗作用。
基金supported by funding from the National Natural Science Foundation of China (Nos. 81672004, 31270202,81801993, and 81801994)the Jilin University Science and Technology Innovative Research Team (JLUSTIRT, 2017TD-05)+3 种基金the Science and Technology Department of Jilin Province (20160101044JC)the Health and Family Planning Commission of Jilin Province (2013Z066)the Key Laboratory of Molecular Virology, Jilin Province (20102209)China Postdocotoral Science Foundation (2018M631869)
文摘RNA modifications are abundant in eukaryotes, bacteria, and archaea. N^6-methyladenosine(m^6A), a type of RNA modification mainly found in messenger RNA(mRNA), has significant effects on the metabolism and function of m RNAs. This modification is governed by three types of proteins, namely methyltransferases as ‘‘writers' ', demethylases as ‘‘erasers' ',and specific m^6A-binding proteins(YTHDF1-3) as ‘‘readers' '. Further, it is important for the regulation of cell fate and has a critical function in many biological processes including virus replication, stem cell differentiation, and cancer development, and exerts its effect by controlling gene expression. Herein, we summarize recent advances in research on m^6A in virus replication and T cell regulation, which is a rapidly emerging field that will facilitate the development of antiviral therapies and the study of innate immunity.
