Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSP...Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.展开更多
随着数字政府、智慧税务的全面推进,税务部门通过使用现代化信息技术手段,实现税务数字化转型从而进行税收征管体制改革。RPA(robotic process automation)技术可依托“流程全自动化”的优势,配合“数据+规则”应用,推动税务征管模式改...随着数字政府、智慧税务的全面推进,税务部门通过使用现代化信息技术手段,实现税务数字化转型从而进行税收征管体制改革。RPA(robotic process automation)技术可依托“流程全自动化”的优势,配合“数据+规则”应用,推动税务征管模式改革,实现税收征管数字化转型。基于当前RPA技术在税务部门的应用,文章总结当前征管体制的主要现状,探讨RPA技术在基层税务的价值和可行性,深化RPA技术在税务数字化转型具有深刻意义。展开更多
操作票系统是电力系统的重要组成部分,是保证倒闸操作的必备安全条件。传统的操作票开票方式以人工开票为主,开票全流程需机械化、重复性地点击操作,流程烦琐且无法简化,批量开票时易疲劳导致开票出错,且工作效率低。为解决上述问题,提...操作票系统是电力系统的重要组成部分,是保证倒闸操作的必备安全条件。传统的操作票开票方式以人工开票为主,开票全流程需机械化、重复性地点击操作,流程烦琐且无法简化,批量开票时易疲劳导致开票出错,且工作效率低。为解决上述问题,提出一种基于RPA(Robotic Process Automation,机器人流程自动化)平台的变电运维端智能开票方法,该方法利用RPA代替人工开票并已在班组实际工作中投入使用,经实践证明,所提方法可有效缩短操作票开票时间,并降低操作票开票出错率,切实达到提质增效。展开更多
基金supported by the Scientific and Innovative Action Plan of Shanghai(21N31900800)Shanghai Rising-Star Program(23QB1403500)+4 种基金the Shanghai Sailing Program(20YF1443000)Shanghai Science and Technology Commission,the Belt and Road Project(20310750500)Talent Project of SAAS(2023-2025)Runup Plan of SAAS(ZP22211)the SAAS Program for Excellent Research Team(2022(B-16))。
文摘Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.
文摘随着数字政府、智慧税务的全面推进,税务部门通过使用现代化信息技术手段,实现税务数字化转型从而进行税收征管体制改革。RPA(robotic process automation)技术可依托“流程全自动化”的优势,配合“数据+规则”应用,推动税务征管模式改革,实现税收征管数字化转型。基于当前RPA技术在税务部门的应用,文章总结当前征管体制的主要现状,探讨RPA技术在基层税务的价值和可行性,深化RPA技术在税务数字化转型具有深刻意义。
文摘操作票系统是电力系统的重要组成部分,是保证倒闸操作的必备安全条件。传统的操作票开票方式以人工开票为主,开票全流程需机械化、重复性地点击操作,流程烦琐且无法简化,批量开票时易疲劳导致开票出错,且工作效率低。为解决上述问题,提出一种基于RPA(Robotic Process Automation,机器人流程自动化)平台的变电运维端智能开票方法,该方法利用RPA代替人工开票并已在班组实际工作中投入使用,经实践证明,所提方法可有效缩短操作票开票时间,并降低操作票开票出错率,切实达到提质增效。