为了考察甲醇或乙醇促进植物生长与赤霉素(GA)的合成关系,该研究在MS固体培养基中培养并添加外源GA和GA合成抑制剂多效唑(PAC),分析其对2mmol/L甲醇或乙醇促进烟草生长的影响及GA合成调控转录因子RSG(for repression of shoot growth)...为了考察甲醇或乙醇促进植物生长与赤霉素(GA)的合成关系,该研究在MS固体培养基中培养并添加外源GA和GA合成抑制剂多效唑(PAC),分析其对2mmol/L甲醇或乙醇促进烟草生长的影响及GA合成调控转录因子RSG(for repression of shoot growth)应答甲醇或乙醇刺激的分子机理。结果显示:(1)外源添加GA可增强甲醇或乙醇对烟草生长的刺激作用,而添加PAC却抑制甲醇和乙醇对烟草生长的刺激作用。(2)14-3-3蛋白与RSG结合抑制RSG进入细胞核及其转录调控活性;甲醇和乙醇诱导烟草14-3-3基因的转录和表达,对RSG蛋白表达也有诱导作用。(3)甲醇和乙醇可降低14-3-3蛋白与RSG的相互作用,同时增强RSG与GA20ox1启动子的结合。研究表明,甲醇和乙醇刺激烟草的生长可能通过增加RSG表达,且减弱RSG与14-3-3蛋白的结合来增加RSG细胞核定位作用,从而增强RSG与GA20ox1启动子的结合,最终增加GA的合成,从而促进烟草的生长,这可能是甲醇和乙醇促进烟草生长的一种重要的分子机制。展开更多
Using Western blot and immunohistochemistry analysis, here the localization ofRSG6 protein was determined in various tissues of rice. Western blot showed only a weak signal in mature pollen. Nevertheless, according to...Using Western blot and immunohistochemistry analysis, here the localization ofRSG6 protein was determined in various tissues of rice. Western blot showed only a weak signal in mature pollen. Nevertheless, according to the result of immunohistochemistry with DAB and fluorescent staining, the expression ofRSG6 protein appeared to begin at the bicellular microspore stage, and then keep activity in the sperm cells of mature pollens. The fluorescence pattern showedRSG6 polypeptide was present close to or attached to the surface of the isolated sperm cells. And this suggested thatRSG6 might take an important part in the process of recognition of sperm cell and ovum. Key words Western blot - immunohistochemistry - location - protein - RSG6 - rice CLC number Q 939.91 Foundation item: Supported by the National Natural Science Foundation of China (30270090); the Doctoral Foundation of the Ministry of Education (98061001) and the Key Project of Natural Science Foundation of Sichuan Province.Biography: Lan Li-qiong (1967-) female, Ph. D; research direction: plant developmental biology and reproductive engineering.展开更多
文摘为了考察甲醇或乙醇促进植物生长与赤霉素(GA)的合成关系,该研究在MS固体培养基中培养并添加外源GA和GA合成抑制剂多效唑(PAC),分析其对2mmol/L甲醇或乙醇促进烟草生长的影响及GA合成调控转录因子RSG(for repression of shoot growth)应答甲醇或乙醇刺激的分子机理。结果显示:(1)外源添加GA可增强甲醇或乙醇对烟草生长的刺激作用,而添加PAC却抑制甲醇和乙醇对烟草生长的刺激作用。(2)14-3-3蛋白与RSG结合抑制RSG进入细胞核及其转录调控活性;甲醇和乙醇诱导烟草14-3-3基因的转录和表达,对RSG蛋白表达也有诱导作用。(3)甲醇和乙醇可降低14-3-3蛋白与RSG的相互作用,同时增强RSG与GA20ox1启动子的结合。研究表明,甲醇和乙醇刺激烟草的生长可能通过增加RSG表达,且减弱RSG与14-3-3蛋白的结合来增加RSG细胞核定位作用,从而增强RSG与GA20ox1启动子的结合,最终增加GA的合成,从而促进烟草的生长,这可能是甲醇和乙醇促进烟草生长的一种重要的分子机制。
文摘Using Western blot and immunohistochemistry analysis, here the localization ofRSG6 protein was determined in various tissues of rice. Western blot showed only a weak signal in mature pollen. Nevertheless, according to the result of immunohistochemistry with DAB and fluorescent staining, the expression ofRSG6 protein appeared to begin at the bicellular microspore stage, and then keep activity in the sperm cells of mature pollens. The fluorescence pattern showedRSG6 polypeptide was present close to or attached to the surface of the isolated sperm cells. And this suggested thatRSG6 might take an important part in the process of recognition of sperm cell and ovum. Key words Western blot - immunohistochemistry - location - protein - RSG6 - rice CLC number Q 939.91 Foundation item: Supported by the National Natural Science Foundation of China (30270090); the Doctoral Foundation of the Ministry of Education (98061001) and the Key Project of Natural Science Foundation of Sichuan Province.Biography: Lan Li-qiong (1967-) female, Ph. D; research direction: plant developmental biology and reproductive engineering.
