We have established an IL-2 independent malignant lymphoma line (CM-1) from peripheral T lymphocytes donated by a femalc patient with nervous systcm disease, the binlogical characteristics of CM-1 cells was studied in...We have established an IL-2 independent malignant lymphoma line (CM-1) from peripheral T lymphocytes donated by a femalc patient with nervous systcm disease, the binlogical characteristics of CM-1 cells was studied in this paper. Another T lymphocytes,such as peripheral T lymphocytes donated by a maIe patient with multiple sclerosis, could be transformed into a malignant lymphoma line by using filtered supernatant of the CM-1 cultured medium, thus the CM-2 cell line u'as estabIished. The CM-1 and CM-2 cells were transplanted by subcutaneous inoculation into nude mice, and could cause the occurrenceof typical maIignant lymphoma. The observation of eIectron micrographs suggested the existence of virions in the CM-1 and CM-2 cells, and these virions were similar toretrovirus in the ultra-structure characteristics. lt was found that this virus possesses reverse transcriptase activity. ResuIts obtained from serological assay, molecular hybridization and PCR excluded the existence of other human viruses, which were commonly usedin our laboratory. The unknown virus possesses strong transformation activity, and probably is a new retro virus. Meanwhile, the work on the clone and sequence analysis ofthis virus are being carried out.展开更多
Complete BV light curves of the W UMa type binary RT LMi are presented. From the observations, four times of minimum light were determined. Based on the new times of minimum light and those collected from the literatu...Complete BV light curves of the W UMa type binary RT LMi are presented. From the observations, four times of minimum light were determined. Based on the new times of minimum light and those collected from the literature, changes in the orbital period of the system were found and analyzed with Kalimeris et al.'s method. The result shows that the orbital period possibly oscillates with a cycle of about 64 years and an amplitude of 1.2 × 10-6 days. The present CCD photometric observations reveal that the light curves are obviously asymmetrical, and show a positive O'Connell effect, while the light curves obtained in 1982 exhibit a negative O'Connell effect. The present light curves were analyzed by means of the latest version of the Wilson-Devinney code, which was also used to correct the photometric effects, including the distortion on the radial-velocity curves obtained by Rucinski et al. The following absolute dimensions have been derived: MI -1.28 ±0.08 M⊙, M2 = 0.48 ±0.06 M⊙, R1 = 1.28 ±0.06 R⊙, R2 = 0.83 ±0.05 R⊙, L1 = 1.88±0.12 L⊙, L2 = 0.77±0.08 L⊙, and A = 2.64±0.02 R⊙. The asymmetry of the light curves can be explained by a model with a cool spot on the secondary component. The orbital period modulation can be reproduced by a magnetic activity cycle model of the secondary component with ΔJ = 5.6 × 1046 g cm2 s-1, ΔΩ/Ω = 8.8 × 10-4, and B = 5.1 × 103 G.展开更多
文摘We have established an IL-2 independent malignant lymphoma line (CM-1) from peripheral T lymphocytes donated by a femalc patient with nervous systcm disease, the binlogical characteristics of CM-1 cells was studied in this paper. Another T lymphocytes,such as peripheral T lymphocytes donated by a maIe patient with multiple sclerosis, could be transformed into a malignant lymphoma line by using filtered supernatant of the CM-1 cultured medium, thus the CM-2 cell line u'as estabIished. The CM-1 and CM-2 cells were transplanted by subcutaneous inoculation into nude mice, and could cause the occurrenceof typical maIignant lymphoma. The observation of eIectron micrographs suggested the existence of virions in the CM-1 and CM-2 cells, and these virions were similar toretrovirus in the ultra-structure characteristics. lt was found that this virus possesses reverse transcriptase activity. ResuIts obtained from serological assay, molecular hybridization and PCR excluded the existence of other human viruses, which were commonly usedin our laboratory. The unknown virus possesses strong transformation activity, and probably is a new retro virus. Meanwhile, the work on the clone and sequence analysis ofthis virus are being carried out.
基金the National Natural Science Foundation of China.
文摘Complete BV light curves of the W UMa type binary RT LMi are presented. From the observations, four times of minimum light were determined. Based on the new times of minimum light and those collected from the literature, changes in the orbital period of the system were found and analyzed with Kalimeris et al.'s method. The result shows that the orbital period possibly oscillates with a cycle of about 64 years and an amplitude of 1.2 × 10-6 days. The present CCD photometric observations reveal that the light curves are obviously asymmetrical, and show a positive O'Connell effect, while the light curves obtained in 1982 exhibit a negative O'Connell effect. The present light curves were analyzed by means of the latest version of the Wilson-Devinney code, which was also used to correct the photometric effects, including the distortion on the radial-velocity curves obtained by Rucinski et al. The following absolute dimensions have been derived: MI -1.28 ±0.08 M⊙, M2 = 0.48 ±0.06 M⊙, R1 = 1.28 ±0.06 R⊙, R2 = 0.83 ±0.05 R⊙, L1 = 1.88±0.12 L⊙, L2 = 0.77±0.08 L⊙, and A = 2.64±0.02 R⊙. The asymmetry of the light curves can be explained by a model with a cool spot on the secondary component. The orbital period modulation can be reproduced by a magnetic activity cycle model of the secondary component with ΔJ = 5.6 × 1046 g cm2 s-1, ΔΩ/Ω = 8.8 × 10-4, and B = 5.1 × 103 G.
