Effect of Biostimulants Based on Natural Products on the Growth and Nutritional Value of Bean (Phaseolus vulgaris L.)

Beans (Phaseolus vulgaris L.) are widely grown in Cameroon and play a key role in the fight against food insecurity, malnutrition and poverty. However, its cultivation encounters problems due to abiotic and biotic stresses, which leads to the use of synthetic fertilizers and pesticides, which cause significant damage to the environment and human health due to the presence of synthetics residues in the seeds, pods and in the leaves that are eaten. Promoting the use of natural products is becoming a necessity for organic and eco-responsible agriculture that limits contamination problems and improves people’s purchasing power. This study aims to assess the effect of biostimulants based on natural products on the growth and nutritional value of common bean (Phaseolus vulgaris L.). Bean seedlings from white variety (MEX-142) and red variety (DOR-701) were treated every seven days in the field from their pre-emergence, emergence and growth to their maturation under a randomized block experimental design. Six treatments and three repetitions with the biostimulants based on natural products and controls were thus performed and the agromorphological parameters were measured. After 120 days, the contents of growth biomarkers and defense-related enzymes were evaluated in leaves, while the contents of macromolecules, minerals and antinutrients were evaluated in seeds. These biostimulants significantly increased (P P < 0.0001) of antinutrients including oxalates, phytates, tannins and saponins in seeds compared to controls (T+ and T−). Treatment with biostimulants, in particular BS4, improves the performance of bean plants in the field as well as the biofortification of seeds regardless of the variety.

基于BERT的煤矿事故风险LEC评价与优化研究

为了提高煤矿事故安全评价的准确性和客观性,针对煤矿安全风险评估中常用的LEC评价法可能出现的不客观以及评价过程中L值评价过于复杂的现状,提出了一种基于BERT预训练模型代替人工进行打分并对L值的评价指标进行优化的方法。通过专家打分的事故分析对模型进行训练,得到预测模型,利用预测模型对需要评价的数据进行预测打分,再结合优化后的L值公式得到事故发生可能性的分值。研究结果表明:BERT模型在经过训练后预测效果较好,与专家判断指标的综合重合度高达92.09%,且改良后的L值判断公式综合了环境物体因素、施工操作人员因素和安全管理因素,在判断时可以较为客观地体现出潜在风险发生的可能性,该研究为煤矿行业中作业条件的危险性评估提供了一种新的思路和方法。

Use of Support Vector Regression Based on Mean Impact Value Model to Identify Active Compounds in a Combination of Curcuma longa L.and Glycyrrhiza extracts

A support vector regression based on the mean impact value (MIV) model was constructed to identify the bioactive compounds inhibiting proliferation of HeLa cells in a combination of turmeric (Curcuma longa L.) and liquorice (Glycyrrhiza) extracts. The quantitative chemical fingerprint from 50 batches of turmeric and liquorice extracts was established using high performance liquid chromatography hyphenated to an ultraviolet visible detector. Qualitative results were obtained using ultra performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry. A total of 46 peaks (peaks 1–15 from turmeric and 16–46 from liquorice) were selected as “common peaks” for analysis. The inhibitory effect of the combined extracts on HeLa cells was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. It was found that 15 compounds (peaks: 8, 12, 30, 24, 46, 11, 14, 9, 3, 1, 44, 18, 7, 45 and 43) possessing high absolute MIV exhibited a significant correlation with the cytotoxicity against HeLa cells; most of these have already been confirmed with potential cytotoxicity in previous research. The important potential application of the present model can be extended to help discover active compounds from complex herbal medicine prior to traditional bioassay-guided separation. It is considered that this could be a useful tool for re-developing herbal medicine based on the use of these active compounds. © 2017, Tianjin University and Springer-Verlag Berlin Heidelberg.

A NOTE ON SAMPLE PATH PROPERTIES OF l^p-VALUED GAUSSIAN PROCESSES

The a.s. sample path properties for l p valued Gaussian processes with stationary increments under some more general conditions are established.

Correlation Analysis of SPAD Value with Chlorophyll Content and Economic Yield Traits of Brassica napus L.

[Objective] The aim was to compare differences of SPAD value, chloro- phyll content, agronomic characters, economic characters and yield traits to analyze correlation of SPAD value with other indices and establish regression functions. [Method] Based on 34 Brassica napus L. varieties, SPAD value, chlorophyll content, agronomic characters, economic characters and yield traits were measured and re- gression functions were established according to correlations. [Result] SPAD value, chlorophyll content, agronomic and economic characters and yield traits all achieved significant level in differences among 34 varieties. Specifically, SPAD value was of extremely significant correlation with chlorophyll a and b, total chlorophyll and carotenoid, and the correlation from high to low was chl-b〉chl-z〉chl-a〉chl-x. SPAD value was of significantly positive correlation with total pod number per plant, plant height, seed number per pod, yield per plant and harvest yield, and of insignificant correlation with branch point height, effective branch number, pod density of main stem, and pod length. [Conclusion] It is simple and rapid to predict chlorophyll con- tent, economic characters and yields of Brassica napus L. with SPAD value and re- gression functions.

Effects of Plant Density on Forage Nutritive Value of Whole Plant Corn

In the field experiment, the effects of plant densities (75 000, 112 500 and 150 000 plants ha-1) on forage nutritive value of whole plant corn (WPC) were studied from 1999 to 2001. The results demonstrated that with the increasing of plant density, the forage matter yield per plant corn decreased significantly, while the fresh matter and dry matter per hectare corn increased significantly, and a higher grains yield was gotten at higher plant densities. Forage nutritive quality of whole plant corn was changed as plant density increased, the crude protein (CP), ether extract (EE), crude fiber (CF), nitrogen free extract (NFE) and general energy (GE) yields increased obviously. Increas- ing plant density reasonably with the application of plant growth regulators could improve plant properties, harvest more forage matter, and enhance forage nutritive value of WPC.

Detection of QTLs with Additive Effects, Epistatic Effects, and QTL × Environment Interactions for Zeleny Sedimentation Value Using a Doubled Haploid Population in Cultivated Wheat

In order to understand the genetic basis for Zeleny sedimentation value （ZSV） of wheat, a doubled haploid （DH） population Huapei 3 × Yumai 57 （Yumai 57 is superior to Huapei 3 for ZSV）, and a linkage map consisting of 323 marker loci were used to search QTLs for ZSV. This program was based on mixed linear models and allowed simultaneous mapping of additive effect QTLs, epistatic QTLs, and QTL x environment interactions （QEs）. The DH population and the parents were evaluated for ZSV in three field trials. Mapping analysis produced a total of 8 QTLs and 2 QEs for ZSV with a single QTL explaining 0.64-14.39% of phenotypic variations. Four additive QTLs, 4 pairs of epistatic QTLs, and two QEs collectively explained 46.11% of the phenotypic variation （PVE）. This study provided a precise location of ZSV gene within the Xwmc 93 and GluD1 interval, which was designated as Qzsv-1D. The information obtained in this study should be useful for manipulating the QTLs for ZSV by marker assisted selection （MAS） in wheat breeding programs.

APPROXIMATION ANALYSES FOR FUZZY VALUED FUNCTIONS IN L_1(μ)-NORM BY REGULAR FUZZY NEURAL NETWORKS

By defining fuzzy valued simple functions and giving L1(μ) approximations of fuzzy valued integrably bounded functions by such simple functions, the paper analyses by L1(μ)-norm the approximation capability of four-layer feedforward regular fuzzy neural networks to the fuzzy valued integrably bounded function F : Rn → FcO(R). That is, if the transfer functionσ: R→R is non-polynomial and integrable function on each finite interval, F may be innorm approximated by fuzzy valued functions defined as to anydegree of accuracy. Finally some real examples demonstrate the conclusions.

Acid Value Determination and Pre-Esterification of Crude Euphorbia lathyris L. Oil

There were various problems in the determination of oil acid value of the national standard method, and this paper developed a low cost, simple and effective way to determine the acid value of oil and grease. Furthermore, the esterification of high acid Euphorbia lathyris L. oil (ELO) with methanol could be efficiently catalyzed by hydrochloric acid to produce biodiesel, and the influencing factors such as the amount of catalyst, reaction time, reaction temperature and molar ratio of oil to methanol were also studied. Under the optimized conditions with the oil to methanol molar ratio of 1:30 and a reaction temperature of 70℃, 95.8% oil conversion was obtained within 40 min in the presence of only 2.0 wt% of catalyst. Therefore, the low-cost non-edible Euphorbia lathyris L. oil as a raw material had good potential for the synthesis of biodiesel in industry.

CONTINUITY OF l^2-VALUED TWO-PARAMETER ORNSTEIN UHLENBECK PROCESSES

Let {Y (t);t= (t 1,t 2)≥0}={X k(t 1,t 2);t 1≥0,t 2≥0} ∞ k=1 be a sequence of two parameter Ornstein Uhlenbeck processes (OUP 2) with coefficients α k>0,β k>0 . A Fernique type inequality is established and the sufficient condition for a.s. l 2 continuity of Y(·) is studied by means of the inequality.

Nutritional Value of Lippia nodiflora( L. ) Greene as Feed

To analyze the nutritional value as feed,the aerial stem and leaves of Lippia nodiflora( L.) Greene and Phyla nodiflora( L.) Greene were sampled to measure the nutritional indices. The nutritional indices were also compared with common forage grass,and the development value of L. nodiflora as feed was analyzed. Compared with P. nodiflora,L. nodiflora had obvious fast-growing and high-yield abilities. The contents of crude fat,crude fiber,crude ash,lysine,vitamin C,Ca,and Fe in L. nodiflora were obviously higher than that in P. nodiflora,but the content of crude protein and total P in L. nodiflora were significantly lower than that in P. nodiflora. Compared with common forage feed ryegrass,alfalfa,millet straw and corn straw,L. nodiflora had higher contents of crude fat and crude ash and lower contents of crude fiber and crude protein. Therefore,L. nodiflora had higher development value as feed.

A Medicinal and Edible Plant Crocus sativus L.and Its Therapeutic Effects on Cardiovascular and Cerebrovascular Diseases

This paper introduces the biological characteristics,medicinal value,chemical component,and pharmacological effects of Crocus sativus L.,and explores its therapeutic effects on cardiovascular and cerebrovascular diseases such as angina pectoris and coronary heart disease.

Diagnosis of Premature Senescence of Cotton Using SPAD Value

Field experiments were conducted in 2011 on an experimental farm at the Cotton Research Insti-tute, Chinese Academy of Agricultural Sciences, Anyang, China. We conducted experiments with a “SPAD-502” meter to quickly measure the relative value of chlorophyll content in the leaf blades of field cotton. Our goal was to seek a suitable leaf on a cotton plant to diagnose senescence status of crop plants at later stages of growth. We began by studying the dynamic change rule of the dis-tal-most four leaves of the cotton plant during the entire growth period with two early-maturing cultivars (CCRI 36, CCRI 50) and two mid-maturing cultivars (CCRI 41, SCRC 28). We also examined the effect of different nitrogen and potassium fertilizer rates on SPAD values of the leaves of SCRC 28. Our results suggest that SPAD values of the 1st distal stem leaves from two early cultivars can act as good indicators of senescence status in the plants, if they are measured between 10 d before the boll-opening stage and 10 d after boll opening stage. Differences of SPAD values of the 3rd distal stem leaves of two mid-maturing cultivars measured between about 15 d before the boll opening stage and 15 d after the boll opening stage can also be used to measure senescence status in these cotton cultivars. The conclusion can be used for cotton producer to manage N fertilizer better at later growth stage.

Characteristic finite difference method and application for moving boundary value problem of coupled system

The coupled system of multilayer dynamics of fluids in porous media is to describe the history of oil-gas transport and accumulation in basin evolution.It is of great value in rational evaluation of prospecting and exploiting oil-gas resources.The mathematical model can be described as a coupled system of nonlinear partial differential equations with moving boundary values.A kind of characteristic finite difference schemes is put forward,from which optimal order estimates in l~2 norm are derived for the error in the approximate solutions.The research is important both theoretically and practically for the model analysis in the field,the model numerical method and software development.

一个二元多项式的Mahler测度

将一个二元多项式P(x,y)=(x2+1)y2+2(x2+x)y+x(x2+1)的Mahler测度表达成一些Bloch-Wigner双对数函数的线性和,进而得到其与χ-3的L函数特殊值的有理倍数关系:m(P(x,y))=5/2L′(X_(-3),-1)。

Nutritional Value Evaluation of Saccharum spontaneum L. Germplasm Resources

The contents of dry matter, crude fat, crude protein, neutral detergent fiber, acid detergent fiber, crude ash, Ca and P in 43 Saccharum spontaneum L.germplasms during the vegetative period were determined. Among them, the crude protein, crude fat, neutral detergent fiber, acid detergent fiber, P and Ca contents were treated as the judging indicators. The nutritional value evaluation was carried out with the analytic hierarchy process(AHP). The results showed A49(Guangxi)had the highest nutritional value. Its dry matter, crude protein, crude fat, crude ash,neutral detergent fiber, acid detergent fiber, Ca and P contents were 32.35%,7.20%, 4.06%, 8.07%, 75.81%, 50.72%, 0.23% and 0.16% respectively. While A3(Guangdong) had the lowest nutritional value. Its dry matter, crude protein, crude fat, crude ash, neutral detergent fiber, acid detergent fiber, Ca and P contents were32.24%, 4.62%, 0.51%, 6.38%, 46.40%, 40.02%, 0.15% and 0.09% respectively.The crude protein content differed significantly among different germplasms. A48 had the highest crude protein content(9.11%), and A14 had the lowest crude protein content(3.72%). Based on the evaluation results, the 43 Saccharum spontaneum L.germplasms were divided into 3 groups: high-nutritional value type, moderate-nutritional value type and low-nutritional value type. We hoped to provide a theoretical reference for the application of Saccharum spontaneum L. as a forage grass.

Value Distribution of L-Functions with Rational Moving Targets

We prove some value-distribution results for a class of L-functions with rational moving targets. The class contains Selberg class, as well as the Riemann-zeta function.

Mean Values of the Hardy Sum

Let p≥5 be a prime. For any integer h, the Hardy sum is defined by H(h,p)=sum((-1)^(j+1+[(hj)/p]))from (p-1) to (j=1) which is related to the classical Dedekind sum. The mean values of the Hardy sum in short intervals are studied by using the mean value theorems of Dirichlet L-functions.

急性大脑中动脉M1段闭塞脑梗死患者低剂量rt-PA静脉溶栓后桥接取栓与直接动脉取栓的临床价值

目的探讨急性大脑中动脉M1段闭塞脑梗死患者低剂量rt-PA静脉溶栓后桥接取栓与直接动脉取栓的临床价值。方法选取2021年6月至2022年6月景德镇市第一人民医院收治的80例急性大脑中动脉M1段闭塞脑梗死患者作为研究对象,根据患者及家属意愿分为A组与B组,每组40例。A组行直接动脉取栓治疗,B组行静脉溶栓后桥接动脉取栓治疗。比较两组血管开通率、病死率、不良事件发生率、美国国立卫生院卒中量表(NIHSS)及改良Rankin评分量表(mRS)评分。结果B组血管开通率高于A组,差异有统计学意义(P<0.05)。两组不良事件总发生率比较差异无统计学意义。治疗前、治疗后24 h,两组NIHSS评分比较差异无统计学意义;治疗后3、7 d,B组NIHSS评分均低于B组,差异有统计学意义(P<0.05)。治疗3个月后,B组mRS评分低于A组,差异有统计学意义(P<0.05)。两组病死率比较差异无统计学意义。结论低剂量rt-PA静脉溶栓后桥接动脉取栓治疗急性大脑中动脉M1段闭塞脑梗死安全、有效,可提高血管开通率,且可改善患者预后,值得临床推广应用。

实时荧光定量RT-PCR在麻疹和风疹病毒检测中的应用

目的探讨实时荧光定量RT-PCR在麻疹和风疹病毒检测中的应用效果。方法选取2021年12月—2022年12月盘锦市疾病预防控制中心接收的麻疹与风疹患者76例为研究对象,入院后所有研究对象均接受酶联免疫吸附试验法和实时荧光定量RT-PCR法检测,以检测患者恢复期血免疫球蛋白G(IgG)抗体水平(相对急性期是否4倍升高)为麻疹诊断金标准,对比两种检测方法的检测准确率、不同检测时间的阳性检出率。结果实时荧光定量RT-PCR法总诊断准确率为97.37%;酶联免疫吸附试验法中总诊断准确率为88.16%,比较两种方法的诊断准确率差异有统计学意义(P<0.05)。第1d,实时荧光定量RT-PCR法阳性检出率高于酶联免疫吸附试验法,差异有统计学意义(P<0.05)。第5d,实时荧光定量RT-PCR法阳性检出率低于酶联免疫吸附试验法,差异有统计学意义(P<0.05)。第2d、第3d、第4d实时荧光定量RT-PCR法和酶联免疫吸附试验法阳性检出率对比差异无统计学意义(P>0.05)。结论实时荧光定量RT-PCR对麻疹、风疹检测有一定诊断作用,可以有效提高检测准确率,同时操作简单,灵敏性较高,值得在麻疹与风疹病毒检测中推广。