Labrasol, as a non-ionic surfactant, can enhance the permeation and absorption of drugs, and is extensively used in topical, transdermal, and oral pharmaceutical preparations as an emulsifier and absorption enhancer. ...Labrasol, as a non-ionic surfactant, can enhance the permeation and absorption of drugs, and is extensively used in topical, transdermal, and oral pharmaceutical preparations as an emulsifier and absorption enhancer. Recent studies in our laboratory have indicated that labrasol has a strong absorption enhancing effect on different types of drugs in vitro and in vivo. This study was performed to further elucidate the action mechanism of labrasol on the corneal penetration. In this research, the fluorescein sodium, a marker of passive paracellular transport of tight junction, was selected as the model drug to assess the effect of labrasol on in vitro corneal permeability. To investigate the continuous and real-time influence of labrasol on the membrane permeability and integrity, the Ussing chamber system was applied to monitor the electrophysiological parameters. And, furthermore, we elucidated the effect of labrasol on excised cornea at the molecular level by application of RT-PCR, Western blot, and immunohistochemical staining. The results indicated that labrasol obviously enhance the transcorneal permeability of fluorescein sodium, and the enhancement was realized by interacting with and down-regulating the associated proteins, such as Factin, claudin-1 and β-catenin, which were contributed to cell-cell connections, respectively.展开更多
The proteins from endosperm, root, stem, leaf, leafstalk of Jatropha curcas L. and their calli were hybridized to the seed toxin protein (curcin) of Jatropha curcas L. by western blot. The result showed that the curci...The proteins from endosperm, root, stem, leaf, leafstalk of Jatropha curcas L. and their calli were hybridized to the seed toxin protein (curcin) of Jatropha curcas L. by western blot. The result showed that the curcin was specifically expressed in the endosperm and its calli, while it was not detected in root, stem, leaf, and leafstalk of Jatropha curcas L. and their calli . This study indicated that calli induced from endosperm can be used to produce curcin.展开更多
文摘Labrasol, as a non-ionic surfactant, can enhance the permeation and absorption of drugs, and is extensively used in topical, transdermal, and oral pharmaceutical preparations as an emulsifier and absorption enhancer. Recent studies in our laboratory have indicated that labrasol has a strong absorption enhancing effect on different types of drugs in vitro and in vivo. This study was performed to further elucidate the action mechanism of labrasol on the corneal penetration. In this research, the fluorescein sodium, a marker of passive paracellular transport of tight junction, was selected as the model drug to assess the effect of labrasol on in vitro corneal permeability. To investigate the continuous and real-time influence of labrasol on the membrane permeability and integrity, the Ussing chamber system was applied to monitor the electrophysiological parameters. And, furthermore, we elucidated the effect of labrasol on excised cornea at the molecular level by application of RT-PCR, Western blot, and immunohistochemical staining. The results indicated that labrasol obviously enhance the transcorneal permeability of fluorescein sodium, and the enhancement was realized by interacting with and down-regulating the associated proteins, such as Factin, claudin-1 and β-catenin, which were contributed to cell-cell connections, respectively.
文摘The proteins from endosperm, root, stem, leaf, leafstalk of Jatropha curcas L. and their calli were hybridized to the seed toxin protein (curcin) of Jatropha curcas L. by western blot. The result showed that the curcin was specifically expressed in the endosperm and its calli, while it was not detected in root, stem, leaf, and leafstalk of Jatropha curcas L. and their calli . This study indicated that calli induced from endosperm can be used to produce curcin.
文摘目的对Western Blot和流式细胞术(Flowcytometry,FCM)这两种磷酸化STAT3的检测方法进行比较。方法用不同浓度的白细胞介素5(interleukin 5,IL-5)刺激人早幼粒细胞白血病细胞株HL-60,活化细胞内的STAT3,然后分别用Western Blot和流式细胞术进行检测。结果 Western Blot与流式细胞术的检测结果一致。结论 Western Blot与流式细胞术用于磷酸化STAT3的检测各有优势,可以互为补充。
