The Effects of Radix Ginseng Rubra on cAMP of Experimental Ischemic Myocardium in Rats

The model of SD rats ligated at the proximate left anterior descend (LAD) of coronary was used. The number and dissociation constant of β receptor were studied by using receptor autoradiography to observe the changes in β receptor and the effects of Radix Ginseng Rubra on cAMP in experimental ischemic myocardium. The result showed that the number of binding site in simple ligation group (B max =0.279) was obviously higher than that in sham operation group (B max =0.093) and the dissociation constant of simple ligation group (Kd=12.431) was higher than that of sham operation group (k d=1.319). There was a significant difference between the two groups ( P <0.05). It proved that the number of β receptor was increased and the activity was elevated in myocardial cell membranes after ligation of LAD. The myocardial cAMP level in simple ligation group (1293.96±519.36)×10 -3 nmol/g was much higher than that in sham operation group (774.44±210.55)×10 -3 nmol/g ; but the level of cAMP in ligation group after receiving Radix Ginseng Rubra treatment (805.02±362.48 pm/g) was obviously lower than that in simple ligation group ( P <0.01), which was close to the result of sham operation. The results indicated that Radix Ginseng Rubra could decrease the cAMP level in ischemic myocardium.

Effect of Extracts from Radix Ginseng,Radix Notoginseng and Rhizoma Chuanxiong on Delaying Aging of Vascular Smooth Muscle Cells in Aged Rats

Objective： To observe the effect of extracts from Radix Ginseng, Radix Notoginseng and Rhizoma Chuanxiong （EXT） on delaying vascular smooth muscle cells （VSMCs） aging in aged rats. Methods： VSMCs were obtained by the modified tissue explants technique and were shown to be positive for smooth muscle α-actin （SM-α-actin） by immunohistochemistry staining. VSMCs obtained from the young rats were served as the young control group; VSMCs obtained from the old rats were treated with no drug （the old group）, with low dose extracts （20 mg/L, the EXT low-concentration group） and high dose extracts （40 mg/L, the EXT highconcentration group）, and with Probucal （106 mol/L, the Probucal group） as a positive control. All groups were cultured for 24 h in the medium with 10% serum for 24 h followed by another 24 h in the serum-free medium. At the end of the 48-h culture, the following analyses were performed including determination of senescenceassociated β-galactosidase （SA β-Gal） activity, flow cytometry analysis of cell cycle, real-time quantitative reverse transcription polymerase chain reaction （RT-PCR） analyses of p16, Cyclin D1, cyclin-dependent kinase 4 （CDK4） and retinoblastoma （Rb） mRNA expression, and Western blotting analyses of p16, cyclin D1, CDK4 and phosphoretinoblastoma （pRb） protein expressions. Results： （1） In comparison to the younger rats, VSMCs from aged rats had significantly more SA β-Gal positive cells （P〈0.01） and more cells in S phase （P〈0.05）. VSMCs from the all treated groups showed a significant decrease in both SA β-Gal positive cells （P〈0.05） and S phase （P〈0.05） compared to the old rats. （2） Compared with the young group, VSMCs in the old group had a significant decrease in p16 and Rb mRNA expression and a significant increase in Cyclin D1 and CDK4 mRNA expression. Compared with the old group, VSMCs in the treated groups had a significant increase in p16 and Rb mRNA expression and a significant decrease in Cyclin D1 and CDK4 mRNA expression （P〈0.05）. （3） Compared with the young group, VSMCs in the old group had a significant decrease in p16 protein expression and a significant increase in Cyclin D1, CDK4 and pRb protein expressions （P〈0.05）. Compared with the old group, VSMCs in the treated groups had a significant increase in p16 protein expression and a significant decrease in cyclinD1, CDK4 and pRb protein expressions （P〈0.05）. Conclusions： VSMCs obtained from old rats showed typical signs of cellular senescence and vascular aging. EXT had an effect on delaying senescence of VSMCs in vitro by altering the p16-cyclinD/CDK-Rb pathway.

Efficacy of Renshen(Radix Ginseng) plus Fuzi(Radix Aconiti Lateralis Preparata) on myocardial infarction by enhancing autophagy in rats

OBJECTIVE:To elucidate the protective effects of Renshen(Radix Ginseng)and Fuzi(Radix Aconiti Lateralis Preparata)on myocardial infarction(MI)through regulating myocardial autophagy.METHODS:Thirty-one male Sprague-Dawley rats were randomized into five groups(n=6 or 7 for each).After treatment for 3 weeks,electrocardiogram(ECG)and cardiac function were recorded.Hematoxylin and eosin(HE)staining was used to detect pathological changes in the heart.Enzyme-linked immunosorbent assay(ELISA)was used to detect serum B-type brain natriuretic peptide(BNP),cardiac troponin T(c Tn T),tumor necrosis factor-α(TNF-α),and serum inflammatory cytokines.Metabolomic analysis was used to identify differential biomarkers of MI in rats.Immunohistochemistry and western blotting were used to detect BNP,cTnT,TNF-α,LC3B,Beclin-1,p62,and adenosine monophosphate activated protein kinase(AMPK)expression in cardiac tissue.RESULTS:Fuzi(Radix Aconiti Lateralis Preparata)alone or Renshen(Radix Ginseng)plus Fuzi(Radix Aconiti Lateralis Preparata)markedly ameliorated cardiac dysfunction and abnormal ECGs,demonstrated by decreases in the heart weight/body weight ratio,BNP,and c Tn T.Pro-inflammation cytokine interleukin(IL)-1αsignificantly decreased and anti-inflammatory cytokine IL-10 significantly increased in Renshen(Radix Ginseng)single or Renshen(Radix Ginseng)plus Fuzi(Radix Aconiti Lateralis Preparata)groups compared with the control group.HE results suggested that co-treatment produced a greater reduction in cardiomyocyte cross-sectional area than Renshen(Radix Ginseng)or Fuzi(Radix Aconiti Lateralis Preparata)alone.Renshen(Radix Ginseng)plus Fuzi(Radix Aconiti Lateralis Preparata)reversed these changes to different degrees in MI rats.Furthermore,Renshen(Radix Ginseng)plus Fuzi(Radix Aconiti Lateralis Preparata)down-regulated LC3 B,Beclin-1,and AMPK expression in cardiac tissue and upregulated p62 expression.CONCLUSIONS:Renshen(Radix Ginseng)plus Fuzi(Radix Aconiti Lateralis Preparata)may have a greater effect on heart injury induced by MI in rats than Fuzi(Radix Aconiti Lateralis Preparata)treatment alone,and the underlying mechanism may be associated with the regulation of myocardial autophagy and anti-inflammation effects.These results provide fresh insight into the mechanism of co-treatment with Renshen(Radix Ginseng)and Fuzi(Radix Aconiti Lateralis Preparata)for MI.

Protective Effect of Radix Ginseng on Peroxidation Injury in Myocardium and Erythrocytes of Streptozocin－Induced Diabetic Rats

The authors have investigated the effect ot Radix Ginseng on peroxidation injury in my-ocardium and erythrocytes of streptozocin-induced diabetic rats. In the ginseng administered group (gin-seng solution 0. 2 g/200 g body weight once daily lasting 15-16 days) . there was a significant decreasein the level of fasting blood glucose and lipid peroxide in myocardium and erythrocytes, in comparison withthose of the model group (P<0. 05) . The superoxide dismutase activities in myocardium and erythrocytesof the ginseng group were increased (P<0. 05) . compared with those of the model group and the vitaminE treated group (vitamin E 10 mg/200 g.body weight once daily, lasting 15-16 days) . The results sug-gest that the mechanisms of antiperoxidation injury of ginseng might include the following: (1) loweringthe level of fasting blood glucose, decreasing monosaccharide autooxidation and partially protecting theproduction of free radicals; (2) elevating the activity of enzymatic free radical scavenger in cells, such assuperoxide dismutase, and (3) directly eliminating the superfluous free radicals.

Effects of extracts from Renshen(Radix Ginseng), Sanqi(Radix Notoginseng), and Chuanxiong(Rhizoma Chuanxiong) on F-actin in senescent microvascular endothelial cells

OBJECTIVE: To investigate the effects of extracts from Renshen(Radix Ginseng), Sanqi(Radix Notoginseng), and Chuanxiong(Rhizoma Chuanxiong) on the endothelial actin cytoskeleton in senescent human cardiac microvascular endothelial cells(HCMECs), and to propose the possible mechanism underlying the actions.METHODS: Lentiviral mediated RNA interference was applied to a replicative senescent HCMEC model by knocking down heat shock protein 27(HSP27)gene. Cells were treated with extracts from Renshen(Radix Ginseng), Sanqi(Radix Notoginseng), and Chuanxiong(Rhizoma Chuanxiong) at final concentrations of 50, 100, and 200 mg/L, respectively and with 10 μM resveratrol for 48 h. Untreated cells were used as controls. Senescence was detected by senescence β-galactosidase staining and cell proliferation was analyzed by cell counting kit-8 assays.Secreted nitric oxide levels were detected by nitrate reductase. Morphological changes of F-actin and G-actin were observed by laser scanning confocal microscopy. Protein and gene expression of Factin and HSP27 was detected by western blotting.RESULTS: Compared with the control group, the proportion of senescent HSP27 shRNA cells treated with the extracts was decreased and their proliferation was increased. In the extract intervention group, F-actin around the cell periphery became irregular and jagged fractures formed gradually and then dissipated. Moreover, some dynamic actin stress fiber filaments appeared. The G-actin stretched to the cell periphery and punctate staining was scattered in the cytoplasm. In addition, the mean optical density value of F/G-actin was decreased significantly and the protein expression of F-actin was downregulated.CONCLUSION: The extracts delayed microvascular endothelial cell senescence by downregulating the expression of F-actin through HSP27.

Simultaneous Determination of Saponins in Radix Glycyrrhizae, Notoginseng and Ginseng by High Performance Liquid Chromatography

To establish a method for determining five saponins(notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and ammonium glycyrrhizinate) in Glycyrrhizae, Notoginseng and Ginseng, the high performance liquid chromatography with diode array detector(HPLC-DAD) method was applied to an Inertsil ODS-SP column(4.6 mm×250 mm, 5 μm) with a mobile phase consisting of acetonitrile-0.05% phosphoric acid in a gradient elution manner. The flow rate was 1.0 mL/min. The column temperature was 30 ℃ and the detection wavelengths were 203 nm and 237 nm, respectively. The linear ranges were 0.700,0—7.000,0 μg for R1(r=1.000,0), 0.751,1— 7.511,4 μg for Rg1(r=1.000,0), 0.677,2—6.771,6 μg for Re(r=1.000,0), 0.733,9—7.339,1 μg for Rb1(r= 1.000,0), and 0.540,0—5.399,8 μg for ammonium glycyrrhizinate(r=0.999,9), respectively. In addition, their average recoveries were 100.28%, 105.83%, 104.09%, 99.36% and 98.54%, respectively. The relative standard deviations(RSDs) of precision, reproducibility and recovery were all less than 1.5%. The results indicate that the method is simple, accurate and reproducible so that it can be used for the simultaneous determination of the five saponins in Chinese patent medicines containing the three kinds of herbs.

Effects of Radix Ginseng on microbial infections: a narrative review

OBJECTIVE: To summarized the antimicrobial-like effects of Radix Ginseng, which provide important information to the relevant researchers and clinicians, and will benefit the clinical treatment of infectious diseases.METHODS: PubMed and Google were used to search for and collect scientific publications related to Radix Ginseng and microbial infections. The authors read, classified, and discussed the associated scientific results or evidences, and summarized the corresponding results.RESULTS: In this review, recent studies on the bene-ficial effects of Radix Ginseng extracts on microbial and biofilm infections were reviewed. The importance and significance of Radix Ginseng's beneficial effects are discussed. Evidence for the favorable effects of Radix Ginseng extracts on viral, bacterial,fungal, and parasitic infections and the possible underlying mechanisms are summarized.CONCLUSION: Radix Ginseng might be a promising supplemental remedy for the prevention and treatment of infectious diseases.

冠心生脉丸质量标准提升

目的提升冠心生脉丸质量标准。方法TLC法定性鉴别人参和三七,分析采用硅胶G薄层板,以三氯甲烷-甲醇-水(13∶7∶2)10℃以下放置的下层溶液为展开剂,10%硫酸乙醇溶液为显色剂。HPLC法测定人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1、人参皂苷Rd的含量,分析采用Thermo Accucore-C18色谱柱(4.6 mm×150 mm,2.6μm);流动相乙腈-水,梯度洗脱;体积流量0.8 mL/min;柱温20℃;检测波长203 nm。结果TLC斑点清晰,阴性无干扰。4种成分在各自范围内线性关系良好(R^(2)≥0.9999),平均加样回收率91.21%~106.86%,RSD 0.68%~1.43%。结论该方法专属性、重复性良好,可为冠心生脉丸质量控制提供参考。

人参、知母调控NR2B-CaMKⅡ-AMPAR1通路防治糖尿病认知功能障碍作用研究

目的 观察人参、知母防治糖尿病认知功能障碍(diabetic cognitive impairment,DCI)的作用,并探讨其机制。方法 采用高脂高糖饲料联合腹腔注射链脲佐菌素(STZ)(70 mg/kg)建立DCI小鼠模型,除空白组,造模成功后随机分为模型组、二甲双胍组(30 mg/kg)、多奈哌齐组(30 mg/kg)、人参组(3.6 g/kg)、知母组(10.8 g/kg)、人参知母组(3.6、7.2 g/kg),每组8只。连续灌胃给药30 d,记录各组小鼠体质量和血糖。给药结束用Moriss水迷宫法进行行为学测试,酶联免疫吸附测定(ELISA)法检测小鼠血清中糖化血红蛋白(GHbA1c);苏木素-伊红(HE)染色法观察海马组织病理变化,Western blot法检测NR2B、钙调蛋白激酶Ⅱ(CaMKⅡ)、α-氨基-3-羟基-5-甲基-4-异唑-丙酸谷氨酸受体1(AMPAR1)蛋白的表达。结果 与模型组相比,人参组(3.6 g/kg)、知母组(10.8 g/kg)、人参知母组(3.6、7.2 g/kg)小鼠体质量增长,血糖下降(P<0.05,P<0.01),逃避潜伏期、探索距离显著缩短(P<0.05,P<0.01),站台穿越次数、目标象限游泳时间显著增加(P<0.05,P<0.01),GHbA1c降低(P<0.05,P<0.01),小鼠海马CA1区神经细胞更加整齐紧密,脑组织中NR2B、AMPAR1蛋白表达显著下调(P<0.01),CaMKⅡ蛋白表达显著上调(P<0.01)。结论 人参知母可改善DCI,其机制可能与调控NR2B-CaMKⅡ-AMPAR1通路有关。

A Survey of the History and Applications of American Ginseng(西洋参)

American ginseng(Panax Quinquefolium L),also known as Xi Yang Shen(西洋参 Radix Panacis Quinquefolii),is indigenous to the United States and Canada.Its discovery in the 1700s paved the way for international trade,which boosted the economy of the New World and helped build commercial ties between the US and China.Due to its therapeutic effects,the demand for American ginseng grew steadily,eventually causing the volume of wild ginseng to dwindle.As a result,laws and regulations were introduced requiring farmers and exporters to engage in harvesting practices that would preserve wild American ginseng.Nowadays,wild American ginseng is considered an endangered and protected species.This article discusses the history,properties,and applications of American ginseng to optimize its use and protection.

牛黄-人参药对发展源流与配伍特征探析

自《神农本草经》首次提出“牛黄,人参为使”以来,历代医家对牛黄-人参配伍多有应用与发挥,内外妇儿各科均能找到应用痕迹。但关于牛黄-人参药对的发展源流及配伍特征尚待梳理与明确。通过对历代医籍相关记载的梳理,分析和总结牛黄-人参药对的历史沿革、配伍特征和临床应用,以期为更好地发挥其临床价值和现代开发提供文献参考。

红参的生物活性及综合利用研究概况

红参是人参的炮制品,具有大补元气、复脉固脱、益气摄血的功效。其主要生物活性成分为人参皂苷、多糖、挥发油等,具有抗癌、抗氧化、延缓衰老、抗中枢神经系统退行性病变、抗过敏、抗炎等多种药理作用。近年来国内外对红参及其残渣的综合利用展开了大量的研究。通过对红参的生物活性以及其产品和副产品的开发应用进行系统整理和综述,以期为红参及相关产品的开发提供参考。

红参提取液的制备及其化妆品应用研究

以中药材红参为研究对象,采用水提纯化富集技术制备红参提取液,对其进行皂苷含量检测以及成分分析,同时对其在化妆品应用进行探究。结果显示,红参提取液皂苷含量为4.8 mg/mL,同时含有多种人参皂苷成分。在LPS诱导RAW264.7细胞和C48/80诱导P815肥大细胞炎症模型上,质量分数为0.5%~2%的红参提取液能剂量依赖性地抑制NO产生及白介素8(IL-8)、肿瘤坏死因子TNF-α的表达,促进白介素10(IL-10)的表达,降低肥大细胞脱颗粒以及组胺释放。鸡胚绒毛尿囊膜刺激性实验显示,质量分数10%红参提取液无刺激。

参麦颗粒中红参掺伪研究

目的建立参麦颗粒中红参掺伪的检查方法。方法采用固相萃取-超高效液相色谱串联质谱(SPE-UPLC-MS/MS)法,色谱柱为Shimadzu Shim-pack GIST C18柱(100 mm×2.1 mm,2μm),流动相为乙腈-水(梯度洗脱),流速为0.35 mL/min,柱温为40℃,进样量为5μL。电喷雾离子源、负离子模式(ESI-),多反应监测(MRM)模式。结果拟人参皂苷F11质量浓度在9.030~344.0 ng/mL范围内与峰面积线性关系良好(r=0.9992,n=5),检测限和定量限分别为1.1288,22.8 ng/mL;精密度、稳定性、重复性试验结果的RSD均小于4.0%;平均加样回收率为108.17%,RSD为4.24%(n=6)。以0.051μg/g为掺伪判定限度,3家企业的85批样品中有9批超出限度(检出率为10.59%)。结论所建立的方法简便快速、准确度高、专属性强,可作为参麦颗粒中红参掺伪的检查方法。

参附配伍对心肌细胞减毒增效作用

通过从化学基础、药代动力学、药物代谢、成分间相互作用、生物学机制等方面进行叙述,揭示了参附方配伍的科学内涵。基于用药剂量和分子作用途径发现了参附方的“量-毒-时-效”关系,药效作用主要通过能量代谢途径实现,毒性作用主要是钙超载。分析乌头碱毒效转换窗口,利用基于生理的药代动力学与反向剂量学预测药物组分在体内的毒性阈值,预测中药早期毒性剂量、药物代谢酶和转运体对药物体内过程影响以及药物相互作用等。参附配伍通过药代机制降低乌头碱的浓度,通过毒理机制对抗毒性靶点,起到“七情和合”中的相畏相杀作用。研究初步阐明了参附配伍减毒增效的科学内涵,发展和完善了“七情和合”配伍理论。

Isolation of Organochlorine Pesticide from Ginseng with Supercritical CO_2

The feasibility of removal of the organochlorine pesticides residues of hexachlorocyclohexane(BHC) from radix ginseng with supercritical CO2 was explored. Some factors, such as extraction pressure, extraction temperature, and kinds of co-solvents were investigated. The experimental results indicate that it is possible to reduce BHC residues in radix ginseng to the level of 0.1 × 10^-6 with supercritical CO2 in the presence of suitable amount of co-solvent, such as water.

人参治疗阿尔茨海默病药理作用及机制研究进展

阿尔茨海默病(Alzheimer's disease,AD)是最常见的神经退行性疾病,因大脑逐渐退化而引起记忆、日常功能减退和行为障碍问题,俗称“老年痴呆”。痴呆症患者有多种类型,阿尔茨海默病占比最大,目前尚无逆转其病程的特效药物。近年来,对这种疾病的发病机制及药物尤其是中药及复方治疗方面,逐步取得了实质性进展,利用动物与细胞模型,诸多研究学者已经从不同角度证实了人参药材提取物、有效部位、单体化合物及复方人参制剂治疗AD具有显著的疗效。就近年来国内外关于人参对AD的治疗新进展研究文献进行梳理归纳,为将其开发成治疗阿尔茨海默病和各种类型记忆障碍的健康产品提供参考和科学依据。

不同外源性物质对园参生长影响的研究

目的筛选在人工种植条件下,可诱导人参植株抗性、刺激其产生次级代谢产物的外源性物质。方法基于可诱导的植物防卫反应,对2年生、3年生、4年生人参植株施以水杨酸、壳聚糖、氨基酸等外源性物质,采集人参植株,采用超高效液相色谱-二极管阵列检测器(UPLC-PDA)法测定其主根中次级代谢产物人参皂苷Rg_(1)、Re、Rb_(1)、Rb_(2)、Rb_(3)、Rd的含量,观察处理前后单株鲜重和人参皂苷含量的变化情况。结果验证了生长/分化平衡假说(GDBH),提示增产和优质是一个矛盾;外源性物质氨基酸、水杨酸、壳聚糖可诱导植物防卫反应,启动次级代谢,促进人参皂苷积累。结论水杨酸、壳聚糖和氨基酸3种外源性物质的复合营养是提高园参中人参皂苷含量最优的外源性调节物质。

原发免疫性血小板减少症关键基因与通路的筛选及中药研究

目的:应用生物信息学方法初步筛选原发免疫性血小板减少症(ITP)的关键基因并探索其发病机制,进而预测治疗ITP的潜在中药(TCM)。方法:基于美国国家生物技术信息中心(NCBI)的基因芯片原始数据集GSE80401,以P<0.05及|logFC|≥1条件筛选差异miRNA的标准进行分析,得到ITP的差异miRNA。采用miRTarBase、miRDB和TargetScan进行miRNA靶基因预测,在Genecards数据库搜索ITP的靶点,将预测的上调靶基因和下调靶基因分别与疾病靶点取交集,在此基础上,将映射的靶基因分别通过String数据库和Cytoscape构建PPI网络,筛选核心的靶基因,对核心靶基因在DAVID和Omicsbean数据库上进行GO和KEGG通路富集分析。将关键基因导入医学本体信息检索数据库(Coremine Medical)分析治疗关键基因的TCM。结果:筛选出符合条件的差异基因422个,关键基因17个,包括BCL2L1、CCND1、CD44、CDKN1A、CREB1、GRB2、MAPK1、MAPK8、PIK3R1、CDK2、CAV1、FGF2、IGF1、SMAD2、SMAD4、TLR4和VEGFA,主要涉及蛋白聚糖、FoXO、PI3K-Akt、人T细胞白血病病毒1感染、内分泌抵抗、黏着斑等信号通路。筛选得到包括人参、赤芍、当归、蜂毒、眼镜蛇、补骨脂、地黄、水牛角、火麻仁、菟丝子、五灵脂和金鸡纳皮等在内的具有防治ITP的TCM共12味。TLR4映射的TCM最多,其次是CCND1和VEGFA。在众多的TCM中,人参同时作用于17个靶点,赤芍、当归和蜂毒同时作用于11个靶点,眼镜蛇和补骨脂同时作用于9个和8个靶点,地黄和水牛角同时作用于7个靶点,火麻仁同时作用于4个靶点,菟丝子同时作用于3个靶点,五灵脂同时作用于2个靶点,提示上述药物具有多靶点防治ITP的潜能。结论:基于生信分析初步预测得到ITP的关键致病基因及具有防治作用的TCM,可为相关TCM新药开发提供靶点及研究思路。

红参-制何首乌药对抗衰老作用的HT22细胞和秀丽隐杆线虫模型初步研究

目的初步探讨不同提取工艺下红参-制何首乌药对对神经细胞的抗衰作用,为红参-制何首乌药对在抗衰及抗阿尔茨海默病(AD)作用的应用提供数据支撑。方法分别制备30%和70%乙醇回流提取物,30%和70%乙醇渗漉提取物和水提取物。通过流式细胞术检测不同提取物对HT22细胞中β-半乳糖苷酶含量的影响,初步筛选红参-制何首乌药对神经细胞抗衰效果最佳的提取物,并通过β-半乳糖苷酶染色直观分析该提取物对神经细胞的抗衰效果,用秀丽隐杆线虫寿命试验及瘫痪实验验证其抗衰及抗AD作用。结果红参-制何首乌药对的不同提取方式均对HT22细胞具有显著的抗衰老作用,其中70%乙醇渗漉提取物效果最佳。对其进行多浓度验证,显示具有剂量依赖性的保护作用。浓度为0.2 mg·mL^(-1)的70%乙醇渗漉提取物对秀丽隐杆线虫的寿命有明显的延长作用,浓度为0.008 mg·mL^(-1)的70%乙醇渗漉提取物具有缓解瘫痪的趋势。结论红参-制何首乌药对的70%乙醇渗漉提取物具有明显抗神经细胞衰老的作用,具有抗衰老延长寿命与治疗AD的潜力,应深入研究。