Objective:To study the energy pharmacology of aconite(Radix aconiti lateralis reparata).Methods:Literature induction method was applied to study the energy properties,energy pharmacological connotation,and energy phar...Objective:To study the energy pharmacology of aconite(Radix aconiti lateralis reparata).Methods:Literature induction method was applied to study the energy properties,energy pharmacological connotation,and energy pharmacological effects of aconite(Radix aconiti lateralis reparata).Results:The warm and hot properties of aconite(Radix aconiti lateralis reparata)are exactly its energy properties.The energy pharmacology of aconite(Radix aconiti lateralis reparata)is based on its energy properties,which is different from the modern aconite(Radix aconiti lateralis reparata)pharmacology that is based on the chemical composition of aconite(Radix aconiti lateralis reparata).In addition,the energy pharmacology of aconite(Radix aconiti lateralis reparata)includes not only the traditional energy pharmacology but also the modern energy pharmacology of aconite(Radix aconiti lateralis reparata).The traditional energy pharmacology of aconite(Radix aconiti lateralis reparata)is mainly manifested as the energy effect of aconite(Radix aconiti lateralis reparata)at the whole-body level,warm yang and dissipate cold,whereas the modern energy pharmacology of aconite(Radix aconiti lateralis reparata)is manifested as the physiological and pathological energy effect of warm and thermal energy properties of aconite(Radix aconiti lateralis reparata)at the tissue,cellular,and molecular levels of the body,mainly strengthening the heart,protecting cardiac muscles,dilating blood vessels,and having anti-arrhythmia,anti-shock,anti-inflammatory,analgesic,and antineoplastic effects.The traditional energy pharmacology and modern energy pharmacology of aconite(Radix aconiti lateralis reparata)constitute the basic connotation of aconite(Radix aconiti lateralis reparata)energy pharmacology.For the aconite(Radix aconiti lateralis reparata)energy pharmacology,there is a substantial foundation,its chemical components such as alkaloids.In addition,the toxicity of aconite(Radix aconiti lateralis reparata)is a manifestation of its energy pharmacology.Conclusion:aconite(Radix aconiti lateralis reparata)has energy properties that have substantial foundation.The energy pharmacology of aconite(Radix aconiti lateralis reparata)is based on its high energy and hot properties and includes the traditional energy pharmacology and modern energy pharmacology of aconite(Radix aconiti lateralis reparata).展开更多
OBJECTIVE： To test the synergistic effects of the aqueous extract of Tubeimu （Rhizoma Bolbosternmatis） and Fuzi （Radix Aconiti Lateralis Preparata） on MDA-MB-231 and SKBR3 breast cancer cells.METHODS： A combined...OBJECTIVE： To test the synergistic effects of the aqueous extract of Tubeimu （Rhizoma Bolbosternmatis） and Fuzi （Radix Aconiti Lateralis Preparata） on MDA-MB-231 and SKBR3 breast cancer cells.METHODS： A combined index was created for the effects of Tubeimu （Rhizoma Bolbostemmatis） and Fuzi （Radix Aconiti Lateralis Preparata） extracts. Cell proliferation was performed by trypan blue exclusion and 3-（4,5-dimethylthiazol-2-yl）-5-（3- carboxy- methoxyphenyl）-2-（4-sulfophenyl）-2H-tetrazolium （MTS） assays. Flow cytometry was used to assess cell cycle distribution and apoptosis. Cell migration was determined by wound-healing and transwell assays. Confocal microscopy was used to detect E-cadherin and actin filaments. RESULTS： The aqueous extract from Tubeimu （Rhizoma Bolbostemmatis）and Fuzi （Radix Aconiti lateralis Preparata） exerted synergetic effects on the growth of MDA-MB-231 cells and G1 phase arrest. When exposed to extracts at concentrations of 62.5 ：62.5 and 62.5：31.3 μg/mL, the combination index was 0.83 and 0.74, respectively. Interestingly, 62.5： 31.3 μg/mL of combined drugs enhanced the inhibitory effect of Tubeimu （Rhizoma Bolbostemmatis） on the migration of SKBR3 cells and reduced the stimulative effect of Fuzi （Radix Aconiti Lateralis Preparata） （P 〈 0.01）, in which cells showed an increased expression of E-cadherin and reorganization of actin filaments （P 〈 0.001）. 62.5：62.5 μg/mL extract also synergistically induced apoptosis of MDA-MB-231 cells （P 〈 0.05 and P 〈 0.001）. Acting as the main active ingredients in the extract, tubeimoside I and acetylbenzoylaconine at 10：10μg/ mL and 5：2.5 μg/mL also produced inhibitory effects on the proliferation and migration of cells （P 〈 0.01）. CONCLUSION： Tubeimu （Rhizoma Bolbostemmatis） and Fuzi （Radix Aconiti Lateralis Preparata） extracts had synergic effects on MDA-MB-231 and SKBR3 cells.展开更多
目的观察含乌头、附子、半夏、栝楼的乌附经方对大鼠心肌缺血再灌注损伤的作用,探讨含有十八反药物的方剂用于特定病理状态的可行性。方法雄性Wistar大鼠50只随机分为5组,即:假手术对照组(SH组)、心肌缺血再灌注模型对照组(I/R组)、乌...目的观察含乌头、附子、半夏、栝楼的乌附经方对大鼠心肌缺血再灌注损伤的作用,探讨含有十八反药物的方剂用于特定病理状态的可行性。方法雄性Wistar大鼠50只随机分为5组,即:假手术对照组(SH组)、心肌缺血再灌注模型对照组(I/R组)、乌附经方低剂量组(I/R+JFL组)、乌附经方中剂量组(I/R+JFM组)及乌附经方高剂量组(I/R+JFH组),每组10只。乌附经方低、中、高剂量组分别以0.75、1.50、3.00mL/100g体质量的剂量灌胃14天,浓度500g/L;除SH组外,其他各组结扎冠状动脉左前降支,使心肌缺血30min,再灌注120min。透射电镜观察心肌线粒体的超微结构改变;一步法脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(terminal-deoxynucleotidyl transferase mediated nick end labeling,TUNEL)检测各组缺血心肌细胞凋亡;RT-PCR法测B细胞白血病/淋巴瘤-2基因(B-cell leukemia/lymphoma2,Bcl-2)、与Bcl-2相关的x蛋白(Bcl-2 associated x protein,Bax)的mRNA表达;ELISA检测血清乳酸脱氢酶(lactic dehydrogenase,LDH)、肌酸激酶(creatine kinase,CK)活性,并测定心肌梗死面积的大小。结果与I/R组比较,乌附经方各组均能降低血清中LDH、CK的释放量(且随着剂量的增加LDH、CK的释放量递减),减小心肌梗死面积、降低凋亡指数(P<0.05);经方组凋亡基因Bcl-2表达水平上升,Bax表达水平下降,Bcl-2/Bax比值升高。结论乌附经方预处理可能通过减少细胞凋亡,对心肌缺血再灌注损伤产生保护作用。说明"十八反"不是绝对的,而是相对的,含有相反中药的复方制剂可用于缺血性心肌病等特定的病理状态。展开更多
【目的】观察细辛和细辛配伍附子含药血清对大鼠心室肌细胞L型钙通道的影响,探讨其抗心律失常的作用机制。【方法】连续给药7 d后制备大鼠含药血清,采用急性酶解法获得单个大鼠心室肌细胞,应用全细胞膜片钳技术记录心室肌细胞L型钙通道...【目的】观察细辛和细辛配伍附子含药血清对大鼠心室肌细胞L型钙通道的影响,探讨其抗心律失常的作用机制。【方法】连续给药7 d后制备大鼠含药血清,采用急性酶解法获得单个大鼠心室肌细胞,应用全细胞膜片钳技术记录心室肌细胞L型钙通道电流。【结果】细辛和细辛配伍附子含药血清均能增加大鼠心室肌L型钙通道电流,在0 m V钳制电压下,细辛含药血清使电流增加约(28.36±0.37)%,细辛配伍附子含药血清使电流增加(75.07±0.28)%。细辛含药血清使激活曲线、失活曲线左移,对恢复曲线无明显作用;细辛配伍附子含药血清使激活曲线、失活曲线左移,使失活后恢复时间延长。【结论】细辛配伍附子较单味细辛含药血清对心肌细胞L型钙通道的激活作用更强,表明药物配伍可增加疗效。展开更多
文摘Objective:To study the energy pharmacology of aconite(Radix aconiti lateralis reparata).Methods:Literature induction method was applied to study the energy properties,energy pharmacological connotation,and energy pharmacological effects of aconite(Radix aconiti lateralis reparata).Results:The warm and hot properties of aconite(Radix aconiti lateralis reparata)are exactly its energy properties.The energy pharmacology of aconite(Radix aconiti lateralis reparata)is based on its energy properties,which is different from the modern aconite(Radix aconiti lateralis reparata)pharmacology that is based on the chemical composition of aconite(Radix aconiti lateralis reparata).In addition,the energy pharmacology of aconite(Radix aconiti lateralis reparata)includes not only the traditional energy pharmacology but also the modern energy pharmacology of aconite(Radix aconiti lateralis reparata).The traditional energy pharmacology of aconite(Radix aconiti lateralis reparata)is mainly manifested as the energy effect of aconite(Radix aconiti lateralis reparata)at the whole-body level,warm yang and dissipate cold,whereas the modern energy pharmacology of aconite(Radix aconiti lateralis reparata)is manifested as the physiological and pathological energy effect of warm and thermal energy properties of aconite(Radix aconiti lateralis reparata)at the tissue,cellular,and molecular levels of the body,mainly strengthening the heart,protecting cardiac muscles,dilating blood vessels,and having anti-arrhythmia,anti-shock,anti-inflammatory,analgesic,and antineoplastic effects.The traditional energy pharmacology and modern energy pharmacology of aconite(Radix aconiti lateralis reparata)constitute the basic connotation of aconite(Radix aconiti lateralis reparata)energy pharmacology.For the aconite(Radix aconiti lateralis reparata)energy pharmacology,there is a substantial foundation,its chemical components such as alkaloids.In addition,the toxicity of aconite(Radix aconiti lateralis reparata)is a manifestation of its energy pharmacology.Conclusion:aconite(Radix aconiti lateralis reparata)has energy properties that have substantial foundation.The energy pharmacology of aconite(Radix aconiti lateralis reparata)is based on its high energy and hot properties and includes the traditional energy pharmacology and modern energy pharmacology of aconite(Radix aconiti lateralis reparata).
基金Supported by Funding Program of(National Science Foundation of China(Mechanism of Warming and Relieving Cold Phlegm Therapy Inhibiting Tumor Metastasis from the Field of Biomechanical,No.81503383)Science and Technology Development of Tianjin Municipal Higher Education(Compatibility Principle of Warming and Relieving Cold Phlegm Formula for the Treatment of Human Hepatocellular Carcinoma,No.20110105)Tianjin city application basis and cutting-edge technology research program(Effect of Herbs Formula Radix Aconiti Praeparata-Rhizoma Bolbostemmatis on Breast Cancer,No.13JCQNJC13800)
文摘OBJECTIVE： To test the synergistic effects of the aqueous extract of Tubeimu （Rhizoma Bolbosternmatis） and Fuzi （Radix Aconiti Lateralis Preparata） on MDA-MB-231 and SKBR3 breast cancer cells.METHODS： A combined index was created for the effects of Tubeimu （Rhizoma Bolbostemmatis） and Fuzi （Radix Aconiti Lateralis Preparata） extracts. Cell proliferation was performed by trypan blue exclusion and 3-（4,5-dimethylthiazol-2-yl）-5-（3- carboxy- methoxyphenyl）-2-（4-sulfophenyl）-2H-tetrazolium （MTS） assays. Flow cytometry was used to assess cell cycle distribution and apoptosis. Cell migration was determined by wound-healing and transwell assays. Confocal microscopy was used to detect E-cadherin and actin filaments. RESULTS： The aqueous extract from Tubeimu （Rhizoma Bolbostemmatis）and Fuzi （Radix Aconiti lateralis Preparata） exerted synergetic effects on the growth of MDA-MB-231 cells and G1 phase arrest. When exposed to extracts at concentrations of 62.5 ：62.5 and 62.5：31.3 μg/mL, the combination index was 0.83 and 0.74, respectively. Interestingly, 62.5： 31.3 μg/mL of combined drugs enhanced the inhibitory effect of Tubeimu （Rhizoma Bolbostemmatis） on the migration of SKBR3 cells and reduced the stimulative effect of Fuzi （Radix Aconiti Lateralis Preparata） （P 〈 0.01）, in which cells showed an increased expression of E-cadherin and reorganization of actin filaments （P 〈 0.001）. 62.5：62.5 μg/mL extract also synergistically induced apoptosis of MDA-MB-231 cells （P 〈 0.05 and P 〈 0.001）. Acting as the main active ingredients in the extract, tubeimoside I and acetylbenzoylaconine at 10：10μg/ mL and 5：2.5 μg/mL also produced inhibitory effects on the proliferation and migration of cells （P 〈 0.01）. CONCLUSION： Tubeimu （Rhizoma Bolbostemmatis） and Fuzi （Radix Aconiti Lateralis Preparata） extracts had synergic effects on MDA-MB-231 and SKBR3 cells.
文摘目的观察含乌头、附子、半夏、栝楼的乌附经方对大鼠心肌缺血再灌注损伤的作用,探讨含有十八反药物的方剂用于特定病理状态的可行性。方法雄性Wistar大鼠50只随机分为5组,即:假手术对照组(SH组)、心肌缺血再灌注模型对照组(I/R组)、乌附经方低剂量组(I/R+JFL组)、乌附经方中剂量组(I/R+JFM组)及乌附经方高剂量组(I/R+JFH组),每组10只。乌附经方低、中、高剂量组分别以0.75、1.50、3.00mL/100g体质量的剂量灌胃14天,浓度500g/L;除SH组外,其他各组结扎冠状动脉左前降支,使心肌缺血30min,再灌注120min。透射电镜观察心肌线粒体的超微结构改变;一步法脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(terminal-deoxynucleotidyl transferase mediated nick end labeling,TUNEL)检测各组缺血心肌细胞凋亡;RT-PCR法测B细胞白血病/淋巴瘤-2基因(B-cell leukemia/lymphoma2,Bcl-2)、与Bcl-2相关的x蛋白(Bcl-2 associated x protein,Bax)的mRNA表达;ELISA检测血清乳酸脱氢酶(lactic dehydrogenase,LDH)、肌酸激酶(creatine kinase,CK)活性,并测定心肌梗死面积的大小。结果与I/R组比较,乌附经方各组均能降低血清中LDH、CK的释放量(且随着剂量的增加LDH、CK的释放量递减),减小心肌梗死面积、降低凋亡指数(P<0.05);经方组凋亡基因Bcl-2表达水平上升,Bax表达水平下降,Bcl-2/Bax比值升高。结论乌附经方预处理可能通过减少细胞凋亡,对心肌缺血再灌注损伤产生保护作用。说明"十八反"不是绝对的,而是相对的,含有相反中药的复方制剂可用于缺血性心肌病等特定的病理状态。
文摘【目的】观察细辛和细辛配伍附子含药血清对大鼠心室肌细胞L型钙通道的影响,探讨其抗心律失常的作用机制。【方法】连续给药7 d后制备大鼠含药血清,采用急性酶解法获得单个大鼠心室肌细胞,应用全细胞膜片钳技术记录心室肌细胞L型钙通道电流。【结果】细辛和细辛配伍附子含药血清均能增加大鼠心室肌L型钙通道电流,在0 m V钳制电压下,细辛含药血清使电流增加约(28.36±0.37)%,细辛配伍附子含药血清使电流增加(75.07±0.28)%。细辛含药血清使激活曲线、失活曲线左移,对恢复曲线无明显作用;细辛配伍附子含药血清使激活曲线、失活曲线左移,使失活后恢复时间延长。【结论】细辛配伍附子较单味细辛含药血清对心肌细胞L型钙通道的激活作用更强,表明药物配伍可增加疗效。
