Objective: To study the targeted point and mechanism of the function of the blood-activating and stasis-removing Chinese drugs, Paeoniae Radix 801(PR801) in its cardiovascular protective effects and its specific bind...Objective: To study the targeted point and mechanism of the function of the blood-activating and stasis-removing Chinese drugs, Paeoniae Radix 801(PR801) in its cardiovascular protective effects and its specific binding with endothelin 1(ET-1) as well as the dynamics of the two's interactive function by means of using affinity biosensors: IAsys Plus and quartz crystal microbalance (IAQCM). Methods: ET-1 was immobilized on the surfaces of IAQCM by using the new surface modification methods. The PR801 in the solution was detected by modified substrates and the specific binding between PR801 and ET-1 was studied. Results: The curves went up or down after adding PR801.There is specific binding between PR801 and ET-1. The bound mass were 0.458 ng/mm 2 and 133.54 ng/cm 2, respectively. There exists relatively good stability with these two methods. Conclusion: The affinity biosensors: IAQCM can be used to study the interaction mechanism between PR801 and ET-1, providing a new way to study the interaction mechanism of TCM. PR801 can bind ET-1 specifically in the experiments. Therefore, ET-1 is another target that PR801 can bind specifically besides thromboxane A 2.展开更多
The development of lung nodules from intravenously injected Walker 256 (W256) tumor cells was the model system for metastases formation in this experiment. The influence and inhibitory mechanisms of a synthetic Paecni...The development of lung nodules from intravenously injected Walker 256 (W256) tumor cells was the model system for metastases formation in this experiment. The influence and inhibitory mechanisms of a synthetic Paecnia rubra 801 (P801) on occurrence of lung metastases were investigated. The results showed that treatment with P801 alone or in combination with decoction of Huangqi (Astragalus root) can significantly reduce the number of lung nodules. The mechanisms responsible for decreased colonyforming potential in the rats treated with P801 were investigated. ADP-induced or W256 tumor cell-induced platelet aggregation was markedly inhibited by P801. It appears that inhibitory effect of P801 on occurrence of W256 tumor lung nodules is mainly due to reduced blood coagulability and inhibition of platelet activation by tumor cells.展开更多
基金Supported by the National Nature Science Foundation of China No. 90209054
文摘Objective: To study the targeted point and mechanism of the function of the blood-activating and stasis-removing Chinese drugs, Paeoniae Radix 801(PR801) in its cardiovascular protective effects and its specific binding with endothelin 1(ET-1) as well as the dynamics of the two's interactive function by means of using affinity biosensors: IAsys Plus and quartz crystal microbalance (IAQCM). Methods: ET-1 was immobilized on the surfaces of IAQCM by using the new surface modification methods. The PR801 in the solution was detected by modified substrates and the specific binding between PR801 and ET-1 was studied. Results: The curves went up or down after adding PR801.There is specific binding between PR801 and ET-1. The bound mass were 0.458 ng/mm 2 and 133.54 ng/cm 2, respectively. There exists relatively good stability with these two methods. Conclusion: The affinity biosensors: IAQCM can be used to study the interaction mechanism between PR801 and ET-1, providing a new way to study the interaction mechanism of TCM. PR801 can bind ET-1 specifically in the experiments. Therefore, ET-1 is another target that PR801 can bind specifically besides thromboxane A 2.
文摘The development of lung nodules from intravenously injected Walker 256 (W256) tumor cells was the model system for metastases formation in this experiment. The influence and inhibitory mechanisms of a synthetic Paecnia rubra 801 (P801) on occurrence of lung metastases were investigated. The results showed that treatment with P801 alone or in combination with decoction of Huangqi (Astragalus root) can significantly reduce the number of lung nodules. The mechanisms responsible for decreased colonyforming potential in the rats treated with P801 were investigated. ADP-induced or W256 tumor cell-induced platelet aggregation was markedly inhibited by P801. It appears that inhibitory effect of P801 on occurrence of W256 tumor lung nodules is mainly due to reduced blood coagulability and inhibition of platelet activation by tumor cells.
