[Objective] The paper was to analyze the meteorological epidemic factors for occurrence and prevalence of tobacco bacterial wilt （ Ralstonia solanaca- rum）, and to study control effects of different soil conditioner...[Objective] The paper was to analyze the meteorological epidemic factors for occurrence and prevalence of tobacco bacterial wilt （ Ralstonia solanaca- rum）, and to study control effects of different soil conditioners on the bacterial disease in Gacligongshan demonstration area of green, ecological, high quality tobac- co leaf production. [Method] The plots attacked by tobacco bacterial wilt over the years were selected and the incidence of the disease was periodically surveyed in tobacco growth period in 2012, 2103 and 2014, respectively. 10 d Effective accumulated temperature and rainfall were counted according to the meteorological data, and the relationship between meteorological factors and disease index was analyzed. The control effects of three kinds of soil conditioners ＂Zhuanggenfeng＂, refined fulvic acid and lime on tobacco bacterial wilt were tested. [ Result] The analysis results of meteorological factors showed that 10 d effective accumulated temperature and rainfall were positively correlated to disease index. The variation curve of 10 d effective accumulated temperature and rainfall reflected the change trend of disease index. The pH values were increased by 0.57, 0.50 and 0.72 respectively after applying ＂Zhuanggenfeng＂, refined fulvic acid and lime. The aver- age control effects on tobacco bacterial wilt were 60.74% -62. 18%, 53.05% -59.53%, and 48.59% -58.53%, respectively. [ Conclusion] 10 d Effective accumulated temperature and rainfall could be used as important reference for disease forecasting and controUing. The usage of soil conditioner has a certain preven- tion and control effect on tobacco bacterial wilt disease by forming soil conditions conducive to flue-cured tobacco growth but adverse to disease survival, which is an effective auxiliary method against the disease.展开更多
【目的】筛选对辣椒青枯病菌生防效果较好的内生细菌,丰富辣椒青枯病的生物防治菌种资源,为生防制剂的研发生产及进行大田生物防治提供理论依据。【方法】在湖南长沙青枯病发病区采集健康的辣椒植株样品,通过平板稀释分离和划线纯化方...【目的】筛选对辣椒青枯病菌生防效果较好的内生细菌,丰富辣椒青枯病的生物防治菌种资源,为生防制剂的研发生产及进行大田生物防治提供理论依据。【方法】在湖南长沙青枯病发病区采集健康的辣椒植株样品,通过平板稀释分离和划线纯化方法获得内生细菌后采用琼脂扩散法进行拮抗作用测定,筛选出拮抗青枯病菌效果较好的内生细菌,然后通过菌体的形态结构观察、生理生化特性试验及16S r DNA序列同源性分析,对筛选出的细菌进行鉴定,采用正交试验对其发酵条件进行优化试验。【结果】筛选出一株对青枯病菌拮抗作用效果好的内生细菌L009,通过对其形态学、生理生化特性鉴定和16S r DNA序列的系统发育鉴定,该菌株为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。通过正交试验,确定该菌株的最佳发酵条件为:玉米淀粉8%、豆饼粉6%、Na Cl 0.5%、Ca CO30.5%、K2HPO40.08%、p H 7.0、72h、28℃、180 r/min转速。【结论】L009菌株对辣椒青枯病菌具有较好的拮抗作用,具有作为生防制剂的潜力。展开更多
基金Supported by Key Science and Technology Project of China National Tobacco Corporation(110201202015)Science and Technology Project of Yunnan Tobacco Company(2012YN11)
文摘[Objective] The paper was to analyze the meteorological epidemic factors for occurrence and prevalence of tobacco bacterial wilt （ Ralstonia solanaca- rum）, and to study control effects of different soil conditioners on the bacterial disease in Gacligongshan demonstration area of green, ecological, high quality tobac- co leaf production. [Method] The plots attacked by tobacco bacterial wilt over the years were selected and the incidence of the disease was periodically surveyed in tobacco growth period in 2012, 2103 and 2014, respectively. 10 d Effective accumulated temperature and rainfall were counted according to the meteorological data, and the relationship between meteorological factors and disease index was analyzed. The control effects of three kinds of soil conditioners ＂Zhuanggenfeng＂, refined fulvic acid and lime on tobacco bacterial wilt were tested. [ Result] The analysis results of meteorological factors showed that 10 d effective accumulated temperature and rainfall were positively correlated to disease index. The variation curve of 10 d effective accumulated temperature and rainfall reflected the change trend of disease index. The pH values were increased by 0.57, 0.50 and 0.72 respectively after applying ＂Zhuanggenfeng＂, refined fulvic acid and lime. The aver- age control effects on tobacco bacterial wilt were 60.74% -62. 18%, 53.05% -59.53%, and 48.59% -58.53%, respectively. [ Conclusion] 10 d Effective accumulated temperature and rainfall could be used as important reference for disease forecasting and controUing. The usage of soil conditioner has a certain preven- tion and control effect on tobacco bacterial wilt disease by forming soil conditions conducive to flue-cured tobacco growth but adverse to disease survival, which is an effective auxiliary method against the disease.
文摘【目的】筛选对辣椒青枯病菌生防效果较好的内生细菌,丰富辣椒青枯病的生物防治菌种资源,为生防制剂的研发生产及进行大田生物防治提供理论依据。【方法】在湖南长沙青枯病发病区采集健康的辣椒植株样品,通过平板稀释分离和划线纯化方法获得内生细菌后采用琼脂扩散法进行拮抗作用测定,筛选出拮抗青枯病菌效果较好的内生细菌,然后通过菌体的形态结构观察、生理生化特性试验及16S r DNA序列同源性分析,对筛选出的细菌进行鉴定,采用正交试验对其发酵条件进行优化试验。【结果】筛选出一株对青枯病菌拮抗作用效果好的内生细菌L009,通过对其形态学、生理生化特性鉴定和16S r DNA序列的系统发育鉴定,该菌株为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。通过正交试验,确定该菌株的最佳发酵条件为:玉米淀粉8%、豆饼粉6%、Na Cl 0.5%、Ca CO30.5%、K2HPO40.08%、p H 7.0、72h、28℃、180 r/min转速。【结论】L009菌株对辣椒青枯病菌具有较好的拮抗作用,具有作为生防制剂的潜力。
