Dengue virus infection:A review of advances in the emerging rapid detection methods

Dengue virus infections are increasing worldwide generally and in Asia,Central and South America and Africa,particularly.It poses a serious threat to the children population.The rapid and accurate diagnostic systems are essentially required due to lack of effective vaccine against dengue virus and the progressive spread of the dengue virus infection.The recent progress in developing micro-and nano-fabrication techniques has led to low cost and scale down the biomedical point-of-care devices.Starting from the conventional and modern available methods for the diagnosis of dengue infection,this review examines several emerging rapid and point-of-care diagnostic devices that hold significant potential for the progress in smart diagnosis tools.The given review revealed that an effective vaccine is required urgently against all the dengue virus serotypes.However,the rapid detection methods of dengue virus help in early treatment and significantly reduce the dengue virus outbreak.

Establishment of a Loop-mediated Isothermal Amplification Method for Rice Bacterial Leaf Brown Spot Disease

Rice bacterial leaf brown spot disease caused by Pseudomonas syringae pv.syringae(Pss)is a major disease on rice.In recent years,Pss has emerged worldwide,seriously affecting rice production.It is very important to establish a rapid detection method of Pss for the diagnosis and prevention of this disease.In order to robust and accurately diagnose the rice bacterial leaf brown spot disease in the field and laboratory,an assay system for the Pss was developed in this study,and the specific sequence of hrcN was used as the target,based on loop-mediated isothermal amplification(LAMP).The best detection system was MgSO 48 mmol·L^(-1),Bst DNA polymerase 8 U,dNTP 1.4 mmol·L^(-1),the ratio of internal and outer primers was 2:1,the reaction temperature was 63℃,the reaction time was 45 min,and the lowest sensitivity was 104 CFU·mL^(-1).This results provided an accurate and robust method for laboratory and field diagnosis of bacterial leaf brown spot disease of rice.

A rapid audio event detection method by adopting 2D-Haar acoustic super feature vector

For accuracy and rapidity of audio event detection in the mass-data audio pro- cessing tasks, a generic method of rapidly recognizing audio event based on 2D-Haar acoustic super feature vector and AdaBoost is proposed. Firstly, it combines certain number of con- tinuous audio frames to be an ＂acoustic feature image＂, secondly, uses AdaBoost.MH or fast Random AdaBoost feature selection algorithm to select high representative 2D-Haar pattern combinations to construct super feature vectors; thirdly, analyzes the commonality and differ- ences between subcategories, then extracts common features and reduces different features to obtain a generic audio event template, which can support the accurate identification of multi- ple sub-classes and detect and locate the specific audio event from the audio stream accurately. Experimental results show that the use of 2D-Haar acoustic feature super vector can make recog- nition accuracy 5% higher than ones that MFCC, PLP, LPCC and other traditional acoustic features yielded, and can make tile training processing 7 20 times faster and the recognition processing 5-10 times faster, it can even achieve an average precision of 93.38%, an average recall of 95.03% under the optimal parameter configuration found by grid method. Above all, it can provide an accurate and fast mass-data processing method for audio event detection.

Advances in the detec

Human health is threatened by foodborne illness and Staphylococcus aureus is a common foodborne pathogenic bacteria.It can cause food poisoning when we are infected,therefore,it is necessary to detect pathogenic bacteria.The virulence genes and detection methods of S.aureus are summarized from literatures.Traditional detection methods are simple but need bacteria enrichment and to prolong detection time.Immunological technology has high specificity,but false positive results are easy to occur in its detection.In recent years,molecular biology methods have developed rapidly,and various PCR techniques have been applied to detect foodborne pathogens.Real-time fluorescent quantitative PCR is more effective than ordinary PCR,while price is expensive when use it,the scope of use is limited.LAMP is a flexible detection method,biosensor and flow cytometry are fast but the cost of detection is high and the price is relatively expensive.