Objective: Detection of loss of heterozygosity (LOH) of RB gene in patients with gastric carcinoma with PCR-VNTR. Methods: We investigated 37 patients with gastric carcinoma. DNA was prepared by standard methods fro...Objective: Detection of loss of heterozygosity (LOH) of RB gene in patients with gastric carcinoma with PCR-VNTR. Methods: We investigated 37 patients with gastric carcinoma. DNA was prepared by standard methods from tumor tissues and normal gastric mucosa (20 cases). The amplification of the VNTR region was performed with PCR. PCR products were run in parallel lanes on 2% agarose gels and 6%PAGE. Results: All the normal gastric mucosa was found to have two different alleles for the VNTR region. Of the 37 cases, LOH was detected in the tumor tissues of 4 patients (10.8%). There was no significantly difference between the high, middle and low, non-differentiation tumor for the LOH of VNTR (P>0.05). Conclusion: PCR-VNTR was a simple and rapid technique for the detection of LOH of RB gene in patients with gastric carcinoma, and it might be useful in the earlier diagnosis of gastric carcinoma.展开更多
Objective:To investigate variable number tandem repeat (VNTR) polymorphism of the 17th intron of Rb gene in Shaanxi aged population and the relationship between the polymorphism of Rb gene and atherosclerosis(AS) gene...Objective:To investigate variable number tandem repeat (VNTR) polymorphism of the 17th intron of Rb gene in Shaanxi aged population and the relationship between the polymorphism of Rb gene and atherosclerosis(AS) genetic suscepti- bility. Methods: VNTR polymorphism of the 17th intron of Rb gene were examined in 100 Shaanxi aged AS patients and 100 Shaanxi aged control individuals by PCR-Rb-Xba Ⅰ-RFLP. Results::Two alleles were found both in AS group and control group, which were separately 945 bp(S1) and 630bp + 315bp(S2). S1S2 genotype was the most frequent one in the two populations. Significant difference in allele frequency was not found between AS group and control group, and allele frequency was no significant difference between Chinese and Caucasian. Conclusion: Xba Ⅰ enzyme site of Rb gene could have been certainly stable in AS population, and it was inferred that the polymorphism locus was not liable to cause mutation, which might not implicated in the formation of AS.展开更多
The structure of the Rb gene in 32 cases of acute lymphoid leukemia (ALL) were studied by Southern blotting using 32P-labeled Rb cDNA 3. 8 kb probe- Structural abnormalities of Rb gene were found in 8 cases of ALL, an...The structure of the Rb gene in 32 cases of acute lymphoid leukemia (ALL) were studied by Southern blotting using 32P-labeled Rb cDNA 3. 8 kb probe- Structural abnormalities of Rb gene were found in 8 cases of ALL, an incidence of 25%. Two novel fragments (3. 1 kb, 2- 3 kb)were observed in 5 of 8 cases. We used five pairs of Rb gene primers of exons 18, 19, 21, 22, 27 and amplified Rb gene from 6 cases of ALL with abnormal Rb gene- Only one case was free from products of exons 18 and 2l. The results seemed to indicate that abnormalities of Rb gene might be closely associated with initiation and/or promotion of ALL.展开更多
The human wild-type Rb gene cDNA has been cis- or trans-inserted into the retrovirus vector DOL, resulting in a sense-expression vector DOLRS and an antisense-expression
Retinoblastoma (Rb) is the most common malignant'cancer of eye.So-Rb_(50) is the first Rb cell line established in China in 1988.It has passed to the 387th passage now.We collected cells of the 327th passage of SO...Retinoblastoma (Rb) is the most common malignant'cancer of eye.So-Rb_(50) is the first Rb cell line established in China in 1988.It has passed to the 387th passage now.We collected cells of the 327th passage of SO-Rb_(50),purified its genomic DNA and detected it with Rb and c-myc cDNA probes respectively(normal human white blood cells DNA was the control).We found the Rb gene was deleted while c-myc gene was amplified three times.This provides a basis for further study of the regulation of tumor development and tumor reversal with this cell line in vitro.Eye Science 1993;9:34-37.展开更多
Retinoblastoma (Rb) is the most common eye cancer in children and it can be inherited. Rb is quite rare and originators from the neural retina with a significant genetic component in etiology, which occurs in approxim...Retinoblastoma (Rb) is the most common eye cancer in children and it can be inherited. Rb is quite rare and originators from the neural retina with a significant genetic component in etiology, which occurs in approximately 1 in every 20 0000 births. In children with the heritable genetic form of Rb, there is a mutation on chromosome 13, called the retinoblastoma 1 (Rb1) gene. Early diagnosis and intervention is critical to the successful treatment of the Rb. The Rb1 gene is the first cloned tumor suppressor gene. As a negative regulator of the cell cycle, Rb1 gene could maintain a balance between cell growth and development through binding to transcription factors and regulating the expression of genes involved in cell proliferation and differentiation. Thus, it is involved in cell cycle, cell senescence, growth arrest, apoptosis and differentiation. We summarized the recent advances on the epidemiology and Rb1 gene of Rb in this review.展开更多
A malignant transformed mammary epithelial cell line (11A1) was transfected with liposome encapsulated eukaryotic expression plasmid pCMV-neo-RB, yielding 4 constant clones which have obvious pheno-typic reversion cha...A malignant transformed mammary epithelial cell line (11A1) was transfected with liposome encapsulated eukaryotic expression plasmid pCMV-neo-RB, yielding 4 constant clones which have obvious pheno-typic reversion changes, and named 11A1-R1-R4 respectively. Further experiments showed that the 11A1-R1 behaved like normal epithelial cells in both morphological and biological characteristics, with decreased clonogenicity in solid argar medium as well as decreased tumorigenicity. Northern blot hybridization showed increased expression of RB gene and decreased expression of c-myc gene in 11A1-R1, 11A1-R2 cells compared to 11A1 cells. This was an ideal phenotypic reversion model for epithelial transformed cell line and demonstrated that the RB gene can reexpress and suppress malignant phenotype in RB inactive cells.展开更多
AIM： TO study the effect of some genes especially those involved in cell cycle regulation on hepatocellular carcinoma. METHODS： Paraffin-embedded tissue samples of 25 patients （18 males and 7 females） with hepatoc...AIM： TO study the effect of some genes especially those involved in cell cycle regulation on hepatocellular carcinoma. METHODS： Paraffin-embedded tissue samples of 25 patients （18 males and 7 females） with hepatocellular carcinoma were collected from 22 pathology centers in Tehran during 2000-2001, and stained using immunohistochemistry method （avidin-biotin-peroxidase） for detection of p53, cyclinD1, RB1, c-los and N-ras proteins. RESULTS： Six （24%）, 5 （20%）, 12 （48%） and 2 samples （8%） were positive for p53, cyclinDl, C-los and N-ras expression, respectively. Twenty-two （88%） samples had alterations in the （31 cell-cycle checkpoint protein expression （RBI or cyclinD1）. P53 positive samples showed a higher （9 times） risk of being positive for RBI protein than p53 negative samples. Loss of expression of RBI in association with p53 over-expression was observed in 4 （66.7%） of 6 samples. Loss of expression of RBI was seen in all cyclinD1 positive, 20 （90.9%） N-ras negative, and ii （50%） C-fos positive samples, respectively. CyclinD1 positive samples showed a higher （2.85 and 4.75 times） risk of being positive for c-los and N-ras expression than cyclinD1 negative samples. CONCLUSION： The expression of p53, RB1 and c-los genes appears to have a key role in the pathogenesis of hepatocellular carcinoma in Iran. Simultaneous overexpression of these genes is significantly associated with their loss of expression during development of hepatocellular carcinoma.展开更多
基金This work was supported by a grant from Hubei Province Education Committee (No.99A063).
文摘Objective: Detection of loss of heterozygosity (LOH) of RB gene in patients with gastric carcinoma with PCR-VNTR. Methods: We investigated 37 patients with gastric carcinoma. DNA was prepared by standard methods from tumor tissues and normal gastric mucosa (20 cases). The amplification of the VNTR region was performed with PCR. PCR products were run in parallel lanes on 2% agarose gels and 6%PAGE. Results: All the normal gastric mucosa was found to have two different alleles for the VNTR region. Of the 37 cases, LOH was detected in the tumor tissues of 4 patients (10.8%). There was no significantly difference between the high, middle and low, non-differentiation tumor for the LOH of VNTR (P>0.05). Conclusion: PCR-VNTR was a simple and rapid technique for the detection of LOH of RB gene in patients with gastric carcinoma, and it might be useful in the earlier diagnosis of gastric carcinoma.
基金National Natural Sciences Foundation of China No. 39700165
文摘Objective:To investigate variable number tandem repeat (VNTR) polymorphism of the 17th intron of Rb gene in Shaanxi aged population and the relationship between the polymorphism of Rb gene and atherosclerosis(AS) genetic suscepti- bility. Methods: VNTR polymorphism of the 17th intron of Rb gene were examined in 100 Shaanxi aged AS patients and 100 Shaanxi aged control individuals by PCR-Rb-Xba Ⅰ-RFLP. Results::Two alleles were found both in AS group and control group, which were separately 945 bp(S1) and 630bp + 315bp(S2). S1S2 genotype was the most frequent one in the two populations. Significant difference in allele frequency was not found between AS group and control group, and allele frequency was no significant difference between Chinese and Caucasian. Conclusion: Xba Ⅰ enzyme site of Rb gene could have been certainly stable in AS population, and it was inferred that the polymorphism locus was not liable to cause mutation, which might not implicated in the formation of AS.
文摘The structure of the Rb gene in 32 cases of acute lymphoid leukemia (ALL) were studied by Southern blotting using 32P-labeled Rb cDNA 3. 8 kb probe- Structural abnormalities of Rb gene were found in 8 cases of ALL, an incidence of 25%. Two novel fragments (3. 1 kb, 2- 3 kb)were observed in 5 of 8 cases. We used five pairs of Rb gene primers of exons 18, 19, 21, 22, 27 and amplified Rb gene from 6 cases of ALL with abnormal Rb gene- Only one case was free from products of exons 18 and 2l. The results seemed to indicate that abnormalities of Rb gene might be closely associated with initiation and/or promotion of ALL.
文摘The human wild-type Rb gene cDNA has been cis- or trans-inserted into the retrovirus vector DOL, resulting in a sense-expression vector DOLRS and an antisense-expression
基金The project was supported by National Natural Science Foundation of China(NAFC)
文摘Retinoblastoma (Rb) is the most common malignant'cancer of eye.So-Rb_(50) is the first Rb cell line established in China in 1988.It has passed to the 387th passage now.We collected cells of the 327th passage of SO-Rb_(50),purified its genomic DNA and detected it with Rb and c-myc cDNA probes respectively(normal human white blood cells DNA was the control).We found the Rb gene was deleted while c-myc gene was amplified three times.This provides a basis for further study of the regulation of tumor development and tumor reversal with this cell line in vitro.Eye Science 1993;9:34-37.
文摘Retinoblastoma (Rb) is the most common eye cancer in children and it can be inherited. Rb is quite rare and originators from the neural retina with a significant genetic component in etiology, which occurs in approximately 1 in every 20 0000 births. In children with the heritable genetic form of Rb, there is a mutation on chromosome 13, called the retinoblastoma 1 (Rb1) gene. Early diagnosis and intervention is critical to the successful treatment of the Rb. The Rb1 gene is the first cloned tumor suppressor gene. As a negative regulator of the cell cycle, Rb1 gene could maintain a balance between cell growth and development through binding to transcription factors and regulating the expression of genes involved in cell proliferation and differentiation. Thus, it is involved in cell cycle, cell senescence, growth arrest, apoptosis and differentiation. We summarized the recent advances on the epidemiology and Rb1 gene of Rb in this review.
文摘A malignant transformed mammary epithelial cell line (11A1) was transfected with liposome encapsulated eukaryotic expression plasmid pCMV-neo-RB, yielding 4 constant clones which have obvious pheno-typic reversion changes, and named 11A1-R1-R4 respectively. Further experiments showed that the 11A1-R1 behaved like normal epithelial cells in both morphological and biological characteristics, with decreased clonogenicity in solid argar medium as well as decreased tumorigenicity. Northern blot hybridization showed increased expression of RB gene and decreased expression of c-myc gene in 11A1-R1, 11A1-R2 cells compared to 11A1 cells. This was an ideal phenotypic reversion model for epithelial transformed cell line and demonstrated that the RB gene can reexpress and suppress malignant phenotype in RB inactive cells.
文摘AIM： TO study the effect of some genes especially those involved in cell cycle regulation on hepatocellular carcinoma. METHODS： Paraffin-embedded tissue samples of 25 patients （18 males and 7 females） with hepatocellular carcinoma were collected from 22 pathology centers in Tehran during 2000-2001, and stained using immunohistochemistry method （avidin-biotin-peroxidase） for detection of p53, cyclinD1, RB1, c-los and N-ras proteins. RESULTS： Six （24%）, 5 （20%）, 12 （48%） and 2 samples （8%） were positive for p53, cyclinDl, C-los and N-ras expression, respectively. Twenty-two （88%） samples had alterations in the （31 cell-cycle checkpoint protein expression （RBI or cyclinD1）. P53 positive samples showed a higher （9 times） risk of being positive for RBI protein than p53 negative samples. Loss of expression of RBI in association with p53 over-expression was observed in 4 （66.7%） of 6 samples. Loss of expression of RBI was seen in all cyclinD1 positive, 20 （90.9%） N-ras negative, and ii （50%） C-fos positive samples, respectively. CyclinD1 positive samples showed a higher （2.85 and 4.75 times） risk of being positive for c-los and N-ras expression than cyclinD1 negative samples. CONCLUSION： The expression of p53, RB1 and c-los genes appears to have a key role in the pathogenesis of hepatocellular carcinoma in Iran. Simultaneous overexpression of these genes is significantly associated with their loss of expression during development of hepatocellular carcinoma.
