A novel optimization algorithm called stochastic focusing search （SFS） for the real-parameter optimization is proposed. The new algorithm is a swarm intelligence algorithm, which is based on simulating the act of hu...A novel optimization algorithm called stochastic focusing search （SFS） for the real-parameter optimization is proposed. The new algorithm is a swarm intelligence algorithm, which is based on simulating the act of human randomized searching, and the human searching behaviors. The algorithm＇s performance is studied using a challenging set of typically complex functions with comparison of differential evolution （DE） and three modified particle swarm optimization （PSO） algorithms, and the simulation results show that SFS is competitive to solve most parts of the benchmark problems and will become a promising candidate of search algorithms especially when the existing algorithms have some difficulties in solving certain problems.展开更多
<strong>Objective: </strong>To determine the prevalence of human metapneumovirus (hMPV) among children with acute respiratory tract infection and the correlation between (hMPV) and age group. <strong>...<strong>Objective: </strong>To determine the prevalence of human metapneumovirus (hMPV) among children with acute respiratory tract infection and the correlation between (hMPV) and age group. <strong>Methods: </strong>This prospective, descriptive cross-sectional hospital-based study is carried out from January to May 2019 among children from under five years old who were admitted to El-Obeid pediatric teaching Hospital, North kordofan, Sudan with acute respiratory tract infection. <strong>Results: </strong>Fifty hospitalized children with acute respiratory tract infection were enrolled in the study. Two of these (4%) were tested positive for (hMPV) in the age group (7 months - 2 years) old. Moreover, the study showed no significant correlation between infection with (hMPV) and age.展开更多
Despite the progress has been reached with Human herpesvirus 8 (HHV-8) research, there are gaps in the knowledge of viral induced oncogenesis. The aim of the present study was to identify possible associations between...Despite the progress has been reached with Human herpesvirus 8 (HHV-8) research, there are gaps in the knowledge of viral induced oncogenesis. The aim of the present study was to identify possible associations between HHV-8 subtypes, HHV-8 loads and clinical manifestations of HIV infected patients diagnosed with different malignancies associated with HHV-8 infection. Forty six HIV-1 infected individuals diagnosed with different HHV-8 associated diseases were studied [37 epidemic Kaposi’s sarcoma (KS), 3 pleural effusion lymphoma (PEL);5 peripheral lymphadenopathies (PL);1 Hodgkin’s lymphoma (HL);1 non Hodgkin’s lymphoma (NHL)]. HHV-8 loads were determined by quantitative real time PCR (qRT-PCR) whilst HHV-8 subtypes were determined by open-reading frame (ORF)-K1 gen genotyping. HHV-8 subtypes B, A, C, A5 and E were exhibited by 31.8%, 23.4%, 19.1%, 17% and 8.5% of the studied patients, respectively. The median HHV-8 viral load did not differ between subtypes (p > 0.05) but HHV-8 viral loads were significantly higher in PEL than in epidemic KS lesion or lymph nodes (p = 0.04). Subtype B was detected in 60% of patients with B cell lymphoma (NHL, PEL and HL) whereas subtype E was only detected in patients with epidemic KS diagnosis. Our data suggest that HHV-8 DNA quantification instead of subtype identification could be used as a surrogate marker for monitoring its infection, not only in epidemic KS patients but also in HIV infected individuals with lymphoproliferative disorders.展开更多
目的分析微滴式数字PCR(ddPCR)和实时荧光定量PCR(qPCR)检测人巨细胞病毒(HCMV)核酸的结果,比较两种方法的差异性,并评估ddPCR技术的临床有效性和实用性。方法收集306例疑似HCMV感染相关疾病患者血浆标本,采用ddPCR和qPCR两种方法测定...目的分析微滴式数字PCR(ddPCR)和实时荧光定量PCR(qPCR)检测人巨细胞病毒(HCMV)核酸的结果,比较两种方法的差异性,并评估ddPCR技术的临床有效性和实用性。方法收集306例疑似HCMV感染相关疾病患者血浆标本,采用ddPCR和qPCR两种方法测定同一份标本的HCMVDNA载量。结果疑似HCMV感染人群中,基于ddPCR的检测结果,男性和女性的阳性率差异无统计学意义(χ^(2)=1.07,P>0.05);不同年龄段的病例阳性率差异无统计学意义(χ^(2)=0.03,P>0.05);不同疾病类型的样本阳性率差异有统计学意义(χ^(2)=10.38,P<0.05)。采用ddPCR方法检测的阳性率为46.41%(120/306),采用qPCR方法检测的阳性率为22.86%(70/306),阳性率差异有统计学意义(χ^(2)=103.80,P<0.05)。受试者工作特征曲线(ROC)分析显示,ddPCR检测的灵敏度为0.96,特异性为0.97,ROC曲线下面积(Area Under Curve,AUC)为0.98。结论采用ddPCR检测HCMVDNA比qPCR具有更好的可定量性,尤其是在低浓度样本检测中更具优势,可以进一步作为临床诊断的直接方法,对现有qPCR检测体系做出有益补充和升级。展开更多
基金supported by the Doctor Students Innovation Foundation of Southwest Jiaotong University.
文摘A novel optimization algorithm called stochastic focusing search （SFS） for the real-parameter optimization is proposed. The new algorithm is a swarm intelligence algorithm, which is based on simulating the act of human randomized searching, and the human searching behaviors. The algorithm＇s performance is studied using a challenging set of typically complex functions with comparison of differential evolution （DE） and three modified particle swarm optimization （PSO） algorithms, and the simulation results show that SFS is competitive to solve most parts of the benchmark problems and will become a promising candidate of search algorithms especially when the existing algorithms have some difficulties in solving certain problems.
文摘<strong>Objective: </strong>To determine the prevalence of human metapneumovirus (hMPV) among children with acute respiratory tract infection and the correlation between (hMPV) and age group. <strong>Methods: </strong>This prospective, descriptive cross-sectional hospital-based study is carried out from January to May 2019 among children from under five years old who were admitted to El-Obeid pediatric teaching Hospital, North kordofan, Sudan with acute respiratory tract infection. <strong>Results: </strong>Fifty hospitalized children with acute respiratory tract infection were enrolled in the study. Two of these (4%) were tested positive for (hMPV) in the age group (7 months - 2 years) old. Moreover, the study showed no significant correlation between infection with (hMPV) and age.
文摘Despite the progress has been reached with Human herpesvirus 8 (HHV-8) research, there are gaps in the knowledge of viral induced oncogenesis. The aim of the present study was to identify possible associations between HHV-8 subtypes, HHV-8 loads and clinical manifestations of HIV infected patients diagnosed with different malignancies associated with HHV-8 infection. Forty six HIV-1 infected individuals diagnosed with different HHV-8 associated diseases were studied [37 epidemic Kaposi’s sarcoma (KS), 3 pleural effusion lymphoma (PEL);5 peripheral lymphadenopathies (PL);1 Hodgkin’s lymphoma (HL);1 non Hodgkin’s lymphoma (NHL)]. HHV-8 loads were determined by quantitative real time PCR (qRT-PCR) whilst HHV-8 subtypes were determined by open-reading frame (ORF)-K1 gen genotyping. HHV-8 subtypes B, A, C, A5 and E were exhibited by 31.8%, 23.4%, 19.1%, 17% and 8.5% of the studied patients, respectively. The median HHV-8 viral load did not differ between subtypes (p > 0.05) but HHV-8 viral loads were significantly higher in PEL than in epidemic KS lesion or lymph nodes (p = 0.04). Subtype B was detected in 60% of patients with B cell lymphoma (NHL, PEL and HL) whereas subtype E was only detected in patients with epidemic KS diagnosis. Our data suggest that HHV-8 DNA quantification instead of subtype identification could be used as a surrogate marker for monitoring its infection, not only in epidemic KS patients but also in HIV infected individuals with lymphoproliferative disorders.
文摘目的分析微滴式数字PCR(ddPCR)和实时荧光定量PCR(qPCR)检测人巨细胞病毒(HCMV)核酸的结果,比较两种方法的差异性,并评估ddPCR技术的临床有效性和实用性。方法收集306例疑似HCMV感染相关疾病患者血浆标本,采用ddPCR和qPCR两种方法测定同一份标本的HCMVDNA载量。结果疑似HCMV感染人群中,基于ddPCR的检测结果,男性和女性的阳性率差异无统计学意义(χ^(2)=1.07,P>0.05);不同年龄段的病例阳性率差异无统计学意义(χ^(2)=0.03,P>0.05);不同疾病类型的样本阳性率差异有统计学意义(χ^(2)=10.38,P<0.05)。采用ddPCR方法检测的阳性率为46.41%(120/306),采用qPCR方法检测的阳性率为22.86%(70/306),阳性率差异有统计学意义(χ^(2)=103.80,P<0.05)。受试者工作特征曲线(ROC)分析显示,ddPCR检测的灵敏度为0.96,特异性为0.97,ROC曲线下面积(Area Under Curve,AUC)为0.98。结论采用ddPCR检测HCMVDNA比qPCR具有更好的可定量性,尤其是在低浓度样本检测中更具优势,可以进一步作为临床诊断的直接方法,对现有qPCR检测体系做出有益补充和升级。
