DFF-EDR:An Indoor Fingerprint Location Technology Using Dynamic Fusion Features of Channel State Information and Improved Edit Distance on Real Sequence

Positioning technology based on wireless network signals in indoor environments has developed rapidly in recent years as the demand for locationbased services continues to increase.Channel state information(CSI)can be used as location feature information in fingerprint-based positioning systems because it can reflect the characteristics of the signal on multiple subcarriers.However,the random noise contained in the raw CSI information increases the likelihood of confusion when matching fingerprint data.In this paper,the Dynamic Fusion Feature(DFF)is proposed as a new fingerprint formation method to remove the noise and improve the feature resolution of the system,which combines the pre-processed amplitude and phase data.Then,the improved edit distance on real sequence(IEDR)is used as a similarity metric for fingerprint matching.Based on the above studies,we propose a new indoor fingerprint positioning method,named DFF-EDR,for improving positioning performance.During the experimental stage,data were collected and analyzed in two typical indoor environments.The results show that the proposed localization method in this paper effectively improves the feature resolution of the system in terms of both fingerprint features and similarity measures,has good anti-noise capability,and effectively reduces the localization errors.

Pooled Plasmid Sequencing Reveals the Relationship Between Mobile Genetic Elements and Antimicrobial Resistance Genes in Clinically Isolated Klebsiella pneumoniae

Plasmids remain important microbial components mediating the horizontal gene transfer(HGT)and dissemination of antimicrobial resistance.To systematically explore the relationship between mobile genetic elements(MGEs)and antimicrobial resistance genes(ARGs),a novel strategy using single-molecule real-time(SMRT)sequencing was developed.This approach was applied to pooled conjugative plasmids from clinically isolated multidrug-resistant(MDR)Klebsiella pneumoniae from a tertiary referral hospital over a 9-month period.The conjugative plasmid pool was obtained from transconjugants that acquired antimicrobial resistance after plasmid conjugation with 53 clinical isolates.The plasmid pool was then subjected to SMRT sequencing,and 82 assembled plasmid fragments were obtained.In total,124 ARGs(responsible for resistance to b-lactam,fluoroquinolone,and aminoglycoside,among others)and 317 MGEs[including transposons(Tns),insertion sequences(ISs),and integrons]were derived from these fragments.Most of these ARGs were linked to MGEs,allowing for the establishment of a relationship network between MGEs and/or ARGs that can be used to describe the dissemination of resistance by mobile elements.Key elements involved in resistance transposition were identified,including IS26,Tn3,IS903 B,ISEcp1,and ISKpn19.As the most predominant IS in the network,a typical IS26-mediated multi-copy composite transposition event was illustrated by tracing its flanking 8-bp target site duplications(TSDs).The landscape of the pooled plasmid sequences highlights the diversity and complexity of the relationship between MGEs and ARGs,underpinning the clinical value of dominant HGT profiles.