期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
The complete genomic sequence analysis of human norovirus NVgz01 strain in Guangzhou
1
作者 QI Yi ZENG JIA YU ZHONG +8 位作者 XIAO LI YAN LI SU BING ZHU YI CHEN TAO LIN MI Sin XIAO HUI YING CHENG RONG ZHOU SI TANG GONG 《Journal of Microbiology and Immunology》 2007年第1期29-34,共6页
The aim of this study is to explore the genomic molecular organization and genogroup of human nomvirus from infected infants in Guangzhou of China. Primers were designed according to the genomic sequence of norovims i... The aim of this study is to explore the genomic molecular organization and genogroup of human nomvirus from infected infants in Guangzhou of China. Primers were designed according to the genomic sequence of norovims in the GenBank, and the nomvirus genome was amplified by RT-PCR. The PCR- products were cloned into T vector and sequenced, and the genomic nucleotide sequences were analyzed with the programs CLUSTAL W/X, DNASTAR and RAT (Recombination Analysis Tool). The NVgz01 strain genome is 7558 bp in length and encodes three open reading frames (GenBank accession No. is DQ369797). The genomic sequences of NVgz01 were compared with those of nomvirus in GenBank, which revealed that the homology with genogroup Ⅱ ranges between 76%-90%, and genogroup Ⅰ between 43%-44%. The ORF1 region shared 94% and 88% identity with Mc37 and Famiington strains, respectively; the capsid region (ORF2) shared 65% and 94% identity with Mc37 and Farmington strains, respectively. Phylogenetic trees were reconstructed by the neighbor-joining method. Comparative complete sequence analysis of the NVgz01 with reported human norovirus genomic sequences revealed that this isolate belongs to genogroup Ⅱ . The ORF1 and ORF2 regions shared different identity with Mc37 and Fannington strains, suggesting NVgz01 could be a recombinant virus. 展开更多
关键词 Human norovirus Genome sequence analysis Recombinant
下载PDF
Comparison of Morphological and Genetic Differentiations in Filial Generation of Cross Between Indica and Japonica Rice
2
作者 MAO Ting XU Hai Xu Quan Guo Yan-hua ZHU Chun-jie CHEN Kai WANG Jia-yu Xu Zheng-jin 《Rice science》 SCIE 2010年第1期82-86,共5页
A recombinant inbred line (RIL) population of F8 and F9 generations derived from a cross between a typical indica rice (Qishanzhan) and a typical japonica rice (Akihikari) was used to study the difference betwee... A recombinant inbred line (RIL) population of F8 and F9 generations derived from a cross between a typical indica rice (Qishanzhan) and a typical japonica rice (Akihikari) was used to study the difference between morphological differentiation based on phenotype characters and genetic differentiation using indica and japonica specific SSR markers, and to evaluate the relationship between vascular bundle characters and morphological and genetic differentiations. The results showed that the frequency distributions of morphological and genetic differentiations were all inclined to japonica type in the filial generation. The population was more inclined to japonica type based on genetic differentiation than on morphological differentiation. The consistent degrees of classification based on the Cheng’s index, the ratio of large vascular bundle number to small vascular bundle number in panicle neck (RLSVB) and the ratio of large vascular bundle number in the second internode from the top to that in the panicle neck (RLVB) were all about 50% compared with the genetic differentiation, and the consistent degree of the total scores of the Cheng’s index combined with the vascular bundle number ratios was significantly increased to about 80% compared with the genetic differentiation. Therefore, the vascular bundle characters could be used as a helpful supplement for subspecies classification. 展开更多
关键词 rice recombinant inbred line Cheng's index vascular bundle characters simple sequence repeat marker subspecies classification method
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部