As there is a strong interest in red-skinned pears,the molecular mechanism of anthocyanin regulation in red-skinned pears has been widely investigated;however,little is known about the molecular mechanism of anthocyan...As there is a strong interest in red-skinned pears,the molecular mechanism of anthocyanin regulation in red-skinned pears has been widely investigated;however,little is known about the molecular mechanism of anthocyanin regulation in red-fleshed pears due to limited availability of such germplasm,primarily found in European pears(Pyrus communis).In this study,based on transcriptomic analysis in red-fleshed and white-fleshed pears,we identified an ethylene response factor(ERF)from P.communis,PcERF5,of which expression level in fruit flesh was significantly correlated with anthocyanin content.We then verified the function of PcERF5 in regulating anthocyanin accumulation by genetic transformation in both pear skin and apple calli.PcERF5 regulated anthocyanin biosynthesis by different regulatory pathways.On the one hand,PcERF5 can activate the transcription of flavonoid biosynthetic genes(PcDFR,PcANS and PcUFGT)and two key transcription factors encoding genes PcMYB10 and PcMYB114.On the other hand,PcERF5 interacted with PcMYB10 to form the ERF5-MYB10 protein complex that enhanced the transcriptional activation of PcERF5 on its target genes.Our results suggested that PcERF5 functioned as a transcriptional activator in regulating anthocyanin biosynthesis,which provides new insights into the regulatory mechanism of anthocyanin biosynthesis.This new knowledge will provide guidance for molecular breeding of red-fleshed pear.展开更多
[Objective]The paper was to investigate and identify new diseases in kiwifruit producing areas in Qiandongnan Prefecture to reduce the harm of diseases and ensure the quality of red-fleshed kiwifruit products.[Method]...[Objective]The paper was to investigate and identify new diseases in kiwifruit producing areas in Qiandongnan Prefecture to reduce the harm of diseases and ensure the quality of red-fleshed kiwifruit products.[Method]The pathogenic fungus was isolated from diseased leaves of redfleshed kiwifruit by tissue separation method,and DNA was sequenced by ribosomal rDNA-ITS(internal transcribed spacer)sequencing.Molecular evolutionary trees were built using MEGA4.0 software,and the pathogenic fungus was classified and identified combined with morphological obser-vation.[Result]Leaf tip blight was a new disease caused by Epicoccum sorghinum.It caused serious damage on red-fleshed kiwifruit.[Conclusion]The study supplements diseases of red-fleshed kiwifruit,and provides support for disease prevention and control in late stage.展开更多
The collection of wild kiwifruit germplasms has already proved its worth with the development of valuable new kiwifruit cultivars.Through field investigation and consulting the existing literature,this paper illustrat...The collection of wild kiwifruit germplasms has already proved its worth with the development of valuable new kiwifruit cultivars.Through field investigation and consulting the existing literature,this paper illustrates that Jiugongshan region in China is a unique gene bank of excellent wild kiwifruit germplasm resources on the boundary between Hubei and Jiangxi provinces.It is also one of the important birthplaces of breeding materials for new kiwifruit cultivars in China.The paper introduces the main characteristics of two wild red-fleshed kiwifruit germplasms discovered in this region,and one of them is a new large-fruited variant of wild red-fleshed kiwifruit.The main fruit qualities of the large-fruited form are that short fruit stalk,large fruit(the maximum fresh fruit mass of about 81.2 g),moderate sweetness and sourness,reddish to red inner pericarp,the ripe fruit soluble solids content 14.10%,total sugar 8.84%,total acid 1.18%,vitamin C 644.3 mg/kg.It is expected to be cultivated into a new edible cultivar(line)or ornamental and edible cultivar(line)of Actinidia chinensis,which has a broad prospect of development and utilization.展开更多
In order to reduce the harm of diseases in red-fleshed kiwifruit in Qiandongnan Prefecture and ensure product quality, a new disease in the producing areas were investigated, and pathogen identification were carried o...In order to reduce the harm of diseases in red-fleshed kiwifruit in Qiandongnan Prefecture and ensure product quality, a new disease in the producing areas were investigated, and pathogen identification were carried out. The pathogen was isolated from diseased leaves by a tissue separation method. DNA sequencing was performed by the sequence analysis of ribosomal rDNA-ITS(Internal transcribed spacer) region, and a molecular evolutionary tree was constructed by the MEGA 4.0 software. The pathogenic fungus was classified and identified by combining morphological observation finally. The results showed that the dry blight disease in leaf tip was a new disease, which was caused by Epicoccum sorghinum, and it had a serious damage to red-fleshed kiwifruit.展开更多
As apple possesses self-incompatibility, knowledge of the S-genotypes is very important for apple breeding and cultivation. We investigated the S-RNase genotypes of 13 type 2 red-fleshed apple cultivars and 8 lineages...As apple possesses self-incompatibility, knowledge of the S-genotypes is very important for apple breeding and cultivation. We investigated the S-RNase genotypes of 13 type 2 red-fleshed apple cultivars and 8 lineages. All of the investigated type 2 red-fleshed apples contained the S3-RNase allele closely linked to the red-fleshed trait. We made a search system for ovule parents to efficiently produce new type 2 red-fleshed cultivars based on the S-RNase allele information(http://www.agr.nagoya-u.ac.jp/～hort/apple/red/). More than 90% of progenies from crossing a selected white-fleshed ovule parent with a type 2 red-fleshed pollen parent are expected to show the red-fleshed phenotype. We also compiled a database of the S-RNase genotypes of more than 1 000 apple cultivars, including a survey system of cultivar combinations showing those that were fully incompatible, semicompatible and fully compatible, written in Chinese(http://www.agr.nagoya-u.ac.jp/～hort/apple/ch/) and Japanese(http://www.agr.nagoya-u.ac.jp/～hort/apple/ja/).展开更多
基金funded by the National Natural Science Foundation of China(31820103012)the earmarked fund for China Agriculture Research System(CARS-28)the earmarked fund for Jiangsu Agricultural Industry Technology System,China(JATS[2022]454).
文摘As there is a strong interest in red-skinned pears,the molecular mechanism of anthocyanin regulation in red-skinned pears has been widely investigated;however,little is known about the molecular mechanism of anthocyanin regulation in red-fleshed pears due to limited availability of such germplasm,primarily found in European pears(Pyrus communis).In this study,based on transcriptomic analysis in red-fleshed and white-fleshed pears,we identified an ethylene response factor(ERF)from P.communis,PcERF5,of which expression level in fruit flesh was significantly correlated with anthocyanin content.We then verified the function of PcERF5 in regulating anthocyanin accumulation by genetic transformation in both pear skin and apple calli.PcERF5 regulated anthocyanin biosynthesis by different regulatory pathways.On the one hand,PcERF5 can activate the transcription of flavonoid biosynthetic genes(PcDFR,PcANS and PcUFGT)and two key transcription factors encoding genes PcMYB10 and PcMYB114.On the other hand,PcERF5 interacted with PcMYB10 to form the ERF5-MYB10 protein complex that enhanced the transcriptional activation of PcERF5 on its target genes.Our results suggested that PcERF5 functioned as a transcriptional activator in regulating anthocyanin biosynthesis,which provides new insights into the regulatory mechanism of anthocyanin biosynthesis.This new knowledge will provide guidance for molecular breeding of red-fleshed pear.
基金Supported by Provincial,Prefecture and College Science and Technology Cooperation Agreement Project of Science and Technology Department of Guizhou Province"Identification and Control Analysis of Kiwifruit Diseases and Insect Pests in Qiandongnan Prefecture:(QKH H[2017]7178)。
文摘[Objective]The paper was to investigate and identify new diseases in kiwifruit producing areas in Qiandongnan Prefecture to reduce the harm of diseases and ensure the quality of red-fleshed kiwifruit products.[Method]The pathogenic fungus was isolated from diseased leaves of redfleshed kiwifruit by tissue separation method,and DNA was sequenced by ribosomal rDNA-ITS(internal transcribed spacer)sequencing.Molecular evolutionary trees were built using MEGA4.0 software,and the pathogenic fungus was classified and identified combined with morphological obser-vation.[Result]Leaf tip blight was a new disease caused by Epicoccum sorghinum.It caused serious damage on red-fleshed kiwifruit.[Conclusion]The study supplements diseases of red-fleshed kiwifruit,and provides support for disease prevention and control in late stage.
文摘The collection of wild kiwifruit germplasms has already proved its worth with the development of valuable new kiwifruit cultivars.Through field investigation and consulting the existing literature,this paper illustrates that Jiugongshan region in China is a unique gene bank of excellent wild kiwifruit germplasm resources on the boundary between Hubei and Jiangxi provinces.It is also one of the important birthplaces of breeding materials for new kiwifruit cultivars in China.The paper introduces the main characteristics of two wild red-fleshed kiwifruit germplasms discovered in this region,and one of them is a new large-fruited variant of wild red-fleshed kiwifruit.The main fruit qualities of the large-fruited form are that short fruit stalk,large fruit(the maximum fresh fruit mass of about 81.2 g),moderate sweetness and sourness,reddish to red inner pericarp,the ripe fruit soluble solids content 14.10%,total sugar 8.84%,total acid 1.18%,vitamin C 644.3 mg/kg.It is expected to be cultivated into a new edible cultivar(line)or ornamental and edible cultivar(line)of Actinidia chinensis,which has a broad prospect of development and utilization.
基金Supported by Province,Prefecture and School Science and Technology Cooperation Agreement Project of Science and Technology Department of Guizhou Province (QKH H Z[2017]7178)The Science and Technologyof Guizhou Province (2017-1166)。
文摘In order to reduce the harm of diseases in red-fleshed kiwifruit in Qiandongnan Prefecture and ensure product quality, a new disease in the producing areas were investigated, and pathogen identification were carried out. The pathogen was isolated from diseased leaves by a tissue separation method. DNA sequencing was performed by the sequence analysis of ribosomal rDNA-ITS(Internal transcribed spacer) region, and a molecular evolutionary tree was constructed by the MEGA 4.0 software. The pathogenic fungus was classified and identified by combining morphological observation finally. The results showed that the dry blight disease in leaf tip was a new disease, which was caused by Epicoccum sorghinum, and it had a serious damage to red-fleshed kiwifruit.
基金supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science(No.16K07594)research funding from the Ogaki Kyoritsu Bank,Ltd.(OKB Agribusiness)the Prize of JSBBA Innovative Research Program Award
文摘As apple possesses self-incompatibility, knowledge of the S-genotypes is very important for apple breeding and cultivation. We investigated the S-RNase genotypes of 13 type 2 red-fleshed apple cultivars and 8 lineages. All of the investigated type 2 red-fleshed apples contained the S3-RNase allele closely linked to the red-fleshed trait. We made a search system for ovule parents to efficiently produce new type 2 red-fleshed cultivars based on the S-RNase allele information(http://www.agr.nagoya-u.ac.jp/～hort/apple/red/). More than 90% of progenies from crossing a selected white-fleshed ovule parent with a type 2 red-fleshed pollen parent are expected to show the red-fleshed phenotype. We also compiled a database of the S-RNase genotypes of more than 1 000 apple cultivars, including a survey system of cultivar combinations showing those that were fully incompatible, semicompatible and fully compatible, written in Chinese(http://www.agr.nagoya-u.ac.jp/～hort/apple/ch/) and Japanese(http://www.agr.nagoya-u.ac.jp/～hort/apple/ja/).
