AIM： To explore the effects of retinoic acid receptor-γ （RARγ） on innate immune responses against Aspergillus fumigatus （A. fumigatus） in cultured human corneal epithelial cells （HCECs）. METHODS： The HCECs ...AIM： To explore the effects of retinoic acid receptor-γ （RARγ） on innate immune responses against Aspergillus fumigatus （A. fumigatus） in cultured human corneal epithelial cells （HCECs）. METHODS： The HCECs were stimulated with A. fumigatus hyphae for 0, 2, 4, 8, 12 and 16h. RARγ mRNA and protein levels were tested by quantitative real-time polymerase chain reaction （qRT-PCR） and Western blot. Then HCECs were pretreated with or without BMS961 （RARγ agonist, 1 μg/mL）. The mRNA and protein expression of Dectin-1 and the downstream cytokines （TNF-α and IL-6） were determined by qRT-PCR, Western blot and enzyme-linked immunosorbent assay （ELISA）. RESULTS： The expression of RARγ was upregulated after stimulation with A. fumigatus. RARγ mRNA began to rise at 4h and peaked at 8h （P〈0.001）. The protein of RARγ reached to the peak at 16h （P〈0.001）. Pretreated with BMS961 before A. fumigatus hyphae stimulation, expression of Dectin-1, TNF-α and IL-6 decreased dramatically at mRNA and protein levels. CONCLUSION： HCECs can express RARγ and A. fumigatus hyphae infection can increase RARγ expression. BMS961 can inhibit the expression of Dectin-1 and pro-inflammatory cytokines, and play an anti-inflammatory role in innate immune responses against A. fumigatus.展开更多
目的探讨三氧化二砷(arsenic trioxide,As2O3)联合全反式维甲酸(all trans retinoic acid,ATRA)在体内和体外对小鼠肝癌H22细胞的抑制作用。方法建立小鼠肝癌H22移植瘤模型,观察As2O3联合ATRA的体内抑瘤作用;用四甲基偶氮唑蓝(MTT)方法...目的探讨三氧化二砷(arsenic trioxide,As2O3)联合全反式维甲酸(all trans retinoic acid,ATRA)在体内和体外对小鼠肝癌H22细胞的抑制作用。方法建立小鼠肝癌H22移植瘤模型,观察As2O3联合ATRA的体内抑瘤作用;用四甲基偶氮唑蓝(MTT)方法测定As2O3联合ATRA在体外对小鼠肝癌H22细胞株的生长抑制作用。结果As2O3联合ATRA在体外能明显抑制H22细胞的生长和增殖,抑制率为52.24%;在体内对H22小鼠的肿瘤体积、重量均有明显的抑制作用,抑制率分别为66.25%、62.07%。结论As2O3和ATRA在体内和体外对H22肿瘤细胞的生长均有抑制作用,联合应用作用加强,有协同作用。展开更多
基金Supported by National Natural Science Foundation of China(No.81470609No.81500695+2 种基金No.81300730)Key Project of Natural Science Foundation of Shandong Province(No.ZR2012HZ001)Youth Natural Science Foundation of Shandong Province(No.ZR2013HQ007)
文摘AIM： To explore the effects of retinoic acid receptor-γ （RARγ） on innate immune responses against Aspergillus fumigatus （A. fumigatus） in cultured human corneal epithelial cells （HCECs）. METHODS： The HCECs were stimulated with A. fumigatus hyphae for 0, 2, 4, 8, 12 and 16h. RARγ mRNA and protein levels were tested by quantitative real-time polymerase chain reaction （qRT-PCR） and Western blot. Then HCECs were pretreated with or without BMS961 （RARγ agonist, 1 μg/mL）. The mRNA and protein expression of Dectin-1 and the downstream cytokines （TNF-α and IL-6） were determined by qRT-PCR, Western blot and enzyme-linked immunosorbent assay （ELISA）. RESULTS： The expression of RARγ was upregulated after stimulation with A. fumigatus. RARγ mRNA began to rise at 4h and peaked at 8h （P〈0.001）. The protein of RARγ reached to the peak at 16h （P〈0.001）. Pretreated with BMS961 before A. fumigatus hyphae stimulation, expression of Dectin-1, TNF-α and IL-6 decreased dramatically at mRNA and protein levels. CONCLUSION： HCECs can express RARγ and A. fumigatus hyphae infection can increase RARγ expression. BMS961 can inhibit the expression of Dectin-1 and pro-inflammatory cytokines, and play an anti-inflammatory role in innate immune responses against A. fumigatus.
文摘目的探讨三氧化二砷(arsenic trioxide,As2O3)联合全反式维甲酸(all trans retinoic acid,ATRA)在体内和体外对小鼠肝癌H22细胞的抑制作用。方法建立小鼠肝癌H22移植瘤模型,观察As2O3联合ATRA的体内抑瘤作用;用四甲基偶氮唑蓝(MTT)方法测定As2O3联合ATRA在体外对小鼠肝癌H22细胞株的生长抑制作用。结果As2O3联合ATRA在体外能明显抑制H22细胞的生长和增殖,抑制率为52.24%;在体内对H22小鼠的肿瘤体积、重量均有明显的抑制作用,抑制率分别为66.25%、62.07%。结论As2O3和ATRA在体内和体外对H22肿瘤细胞的生长均有抑制作用,联合应用作用加强,有协同作用。
