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Study of Hemorrhoid Disease in the Department of General Surgery of the Cs Ref of the Commune I Bamako
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作者 Tounkara Cheickna Diarra Issaka +8 位作者 Sanogo Modibo Togola Modibo Camara Ladji Soma Dembele Bakary Tientigui Traore Alhassane Togo Adégné Pierre Kante Lassana Ouattara Zanafon Diallo Gangaly 《Surgical Science》 2024年第2期36-47,共12页
This work was a retrospective prospective study carried out in the general surgery department of the Cs ref of commune I from January 2009 to December 2012. Our study aimed to determine the hospital frequency of hemor... This work was a retrospective prospective study carried out in the general surgery department of the Cs ref of commune I from January 2009 to December 2012. Our study aimed to determine the hospital frequency of hemorrhoidal disease, to identify the contributing factors, describe the clinical and therapeutic aspects and analyze the surgical consequences. We collected 73 patients with an average age of 37.8 years (range: 19 years and 77 years) with a sex ratio of 2.5 in favor of men. We operated on 49 patients or 67.1% of cases. 71.2% of our patients were married and lived in Bamako. Schoolchildren and middle managers made up 39.8% of cases. 82.2% of patients were seen in ordinary consultation and 69.9% complained of progressive anal pain in 66.7% of cases. This pain was triggered by defecation in 88.2% of our patients. The contributing factors were dominated by constipation (53.4%), working in a seated position (41.1%) and a sedentary lifestyle (16.4%) of cases. Stage IV external hemorrhoids and hemorrhoidal thrombosis represented 53.8% of surgical indications. The most used surgical technique was that of Milligan-Morgan, i.e. 51.9% of cases and 45.2% of patients treated medically received a combination of transit regulator, venotonics and analgesics. The surgical aftermath was marked by hemorrhage (3 cases), delayed healing (5 cases), urinary retention (1 case) and scarring anal stenosis (1 case). The morbidity rate was 5.48% of cases and a zero mortality rate. The average length of hospitalization was 2 days with extremes of 1 and 5 days. 展开更多
关键词 HEMORRHOID Surgery Cs ref C I BAMAKO MALI
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Perception and Management of Hemorroid Disease at the CSREF of the Commune Bamako Mali
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作者 Tounkara Cheickna Diarra Mohamed Hassimy +6 位作者 Diarra Issaka Sanogo Keita Bakary Dembele Bakary Tientigui Traore Alhassane Togo Pierre Adégné Lassana Kante 《Surgical Science》 2024年第2期89-97,共9页
This work was a prospective transversal study over 6 (six) months from May 2 to December 31, 2017 at the Reference Health Center of Commune I and the Chérif la Confessional Health Center in Bamako. The aim was to... This work was a prospective transversal study over 6 (six) months from May 2 to December 31, 2017 at the Reference Health Center of Commune I and the Chérif la Confessional Health Center in Bamako. The aim was to study the knowledge, attitudes and behavioral practices linked to hemorrhoids in Commune I of the Bamako district, to evaluate the frequency of hemorrhoidal disease, to determine the knowledge, attitudes and behavioral practices linked to hemorrhoids among patients, their companions and traditional therapists in Commune I of the district of Bamako and the factors which influence the motivations and decisions to resort to medical-surgical care and traditional medicine in patients suffering from hemorrhoids in order to analyze the results of the management of hemorrhoidal disease at Cs Ref CI. We collected 36 patients with hemorrhoidal disease, 25 caregivers and 15 traditional hemorrhoidal disease therapists. The sex ratio was 1.25 for patients;6.5 for traditional therapists and 1.5 for accompanying people. The average age of the patients was 32.75 years;49 years for traditional therapists and 28.76 years for those accompanying them. 55.6% of patients claim to know about hemorrhoidal disease;100% of traditional therapists and 80% of accompanying people. The dietary factor of hemorrhoidal disease was mentioned by 90% of patients;66.7% of traditional therapists and 100% of caregivers. Most patients initially resorted to traditional treatment out of fear of surgery and its after-effects. The patients were treated medically and surgically. 展开更多
关键词 Hemorrhoidal Disease CAREGIVERS Traditional Therapists Patients Surgery CS ref CI
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Clinical and Therapeutic Aspects of Inguinal Hernia in Children in the General Surgery Department of Reference Health Center in Commune I of Bamako Mali
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作者 Tounkara Cheickna Samake Hamidou +8 位作者 Simpara Mama Diarra Issaka Sanogo Modibo Sidibe Souleymane Togola Modibo Dembele Bakary Tientigui Coulibaly Yacaria Togo Pierre Adégné Kante Lassana 《Surgical Science》 2024年第2期54-63,共10页
Inguinal hernia in children is a congenital pathology in children linked to the persistence of the peritoneo-vaginal canal in children and the NüCK canal in girls;persisting into adulthood. This work aimed to stu... Inguinal hernia in children is a congenital pathology in children linked to the persistence of the peritoneo-vaginal canal in children and the NüCK canal in girls;persisting into adulthood. This work aimed to study inguinal hernia in children in the general surgery department of the Reference Health Center of Commune I of the Bamako District;to determine the frequency of inguinal hernia;describe the epidemiological, clinical and therapeutic aspects of inguinal hernias and in order to evaluate the cost of treatment. This was a prospective study from April 2017 to March 2018 during which 60 children with an inguinal hernia were collected. Inguinal hernias in children represented 9.23% of patients operated on in the department. The average age was 59.23 months with extremes of one month and 180 months and a standard deviation of 49.23 years. The male sex was dominant with a sex ratio equal to 9:1. The notion of prematurity was found in 11.7% and was associated with a testicle not in place in 1.7% of cases. 41.7% of our patients were referred by a doctor and 66.7% of patients presented with painless, intermittent inguino-scrotal swelling (83.3% of cases). The hernia was discovered before one week of life in 50% of cases. Inguinal swelling was absent in 11.7% but observed intraoperatively. The inguinal hernia was unilateral in 91.7% of cases and 70% of hernias were discovered by the parents during pushing efforts. The inguinal hernia was located on the right in 61.7% of cases. The hernia swelling was soft, painless, impulsive and reducible in 78.3% of cases. The swelling was inguino-scrotal in 58.3% of cases. The hernia was simple in 95% of patients and hernial strangulation was observed in 3 cases or 5% of cases. 98.3% of patients were ASA I. The treatment was carried out openly in all our patients including closure of the vaginal peritoneal canal in 95% of cases under general anesthesia in 98.3% of cases. The morbidity rate was 8.4% (surgical site infection: 6.7% of cases and hematoma: 1.7%). The immediate consequences were simple in 96.6% of cases. No cases of recurrence occurred during the 6 months after the intervention. The average cost of care was estimated at 69,743 FCFA. 展开更多
关键词 Inguinal Hernia Child Surgery Cs ref C I Bamako Mali
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Postoperative Complications in the General Surgery Department of the Cs Ref of Commune I of the District of Bamako Mali
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作者 Tounkara Cheickna Samake Hamidou +7 位作者 Dembele Lamine Diarra Issaka Togola Modibo Sanogo Modibo Dembele Bakary Tientigui Traore Alhassane Togo Adégné Pierre Kante Lassana 《Surgical Science》 2024年第6期409-419,共11页
Postoperative complications represent important indicators for the quality of surgical care. The objectives of this work were to study post-operative complications in the general surgery department of the Cs ref of co... Postoperative complications represent important indicators for the quality of surgical care. The objectives of this work were to study post-operative complications in the general surgery department of the Cs ref of commune I in Mali, to determine the frequency of post-operative complications, to identify the factors of occurrence, to describe the clinical aspects, diagnostic and therapeutic in order to assess the additional cost linked to post-operative complications. This study was prospective, carried out in the general surgery department on 300 cases of surgical interventions. It covered all patients aged at least 15 years, operated on and hospitalized or not, and who presented complications during the 30 days postoperatively. Patients under the age of 15 were not included (our general surgery department is not a pediatric surgery department). We collected 300 patients among whom 199 (66.33%) were men and 101 (33.66%) women, i.e. a sex ratio = 1.9. The average age was 38 years with extremes of 15 and 87 years. The main initial diagnoses were: acute appendicitis, peritonitis, occlusions, wall hernias, hemorrhoids, uterine prolapse, uterine myomas, ovarian cysts and acute cholecystitis. Emergencies represented 43% (N = 129) of interventions with 6.98% post-operative complications (POC). Postoperative complications were dominated by surgical site infections, 75% of cases (N = 20), wall hemorrhage 5% (N = 1), testicular necrosis 5% (N = 1) and testicular calcification 5% (N = 1), wire rejection 5% (N = 1) and death 5% (N = 1). The management of postoperative complications was surgical in 95% and medical in 100%. Their occurrence extended the hospital stay by 3.65 days and increased the average cost of care by 60541.85 CFA francs. The mortality index lowered by efficient management of complications (IMAGE) calculated in relation to deaths was 95% of cases. Anemia, ASA score ≥ III, Alteimeir II and IV classes, duration of surgery and post-operative hospitalization were factors contributing to post-operative complications. 展开更多
关键词 Complications Post Opératoires CHIRURGIE Cs ref CI BAMAKO MALI
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基于MW-REF算法的心肺复苏影响因素分析 被引量:2
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作者 张友坤 陈伟 +3 位作者 靳小静 孙洁 李瑞月 张瑛琪 《科学技术与工程》 北大核心 2023年第22期9543-9549,共7页
针对传统模型对心肺复苏结果预测准确率较低、模型可解释性较差,提出了一种基于多模型加权递归消除法(MW-REF)的心肺复苏结果预测模型,并在Shapley加法解释(Shapley additive explanation,SHAP)框架下分析影响心肺复苏结果的关键因素。... 针对传统模型对心肺复苏结果预测准确率较低、模型可解释性较差,提出了一种基于多模型加权递归消除法(MW-REF)的心肺复苏结果预测模型,并在Shapley加法解释(Shapley additive explanation,SHAP)框架下分析影响心肺复苏结果的关键因素。采用了随机森林、GBDT、XGBOOST作为基模型,将其特征重要性得分加权后使用递归消除法过滤特征并对3种及模型采用Voting进行模型融合,利用五折交叉验证下的准确率作为最终特征选择标准。最后对最终特征数据集下的融合模型进行可解释性分析。实验结果表明,与传统的递归特征消除算法对比,该模型提升了心肺复苏结果预测的准确率,模型预测结果具有可解释性,可为临床诊断提供辅助,提高诊断效率与心肺复苏成功率。 展开更多
关键词 多模型加权递归特征消除法 心肺复苏 模型融合 SHAP
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RNA结合蛋白Aly/REF输出因子通过上调Yes相关蛋白表达促进胃癌细胞的增殖和迁移
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作者 李睿 邵爽 +2 位作者 孟玮 金丹 郭纪伟 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2023年第4期622-630,共9页
胃癌是一种常见的发病率高、起病隐匿、侵袭性强的消化道恶性肿瘤,其发病机制错综复杂。RNA结合蛋白Aly/REF输出因子(RNAbinding protein Aly/REF export factor, ALYREF)作为一种RNA胞嘧啶的5-甲基化修饰(m~5C)后的结合蛋白质,在调控RN... 胃癌是一种常见的发病率高、起病隐匿、侵袭性强的消化道恶性肿瘤,其发病机制错综复杂。RNA结合蛋白Aly/REF输出因子(RNAbinding protein Aly/REF export factor, ALYREF)作为一种RNA胞嘧啶的5-甲基化修饰(m~5C)后的结合蛋白质,在调控RNA出核及稳定其表达的过程中具有关键作用;Yes相关蛋白(yes-associated protein, YAP)作为Hippo-YAP信号通路中的关键分子,调控各种恶性肿瘤的生长和转移。本文旨在探讨ALYREF与YAP在促进胃癌细胞增殖和迁移中是否存在相关性,并初步探索这两者之间的作用机制。本文在胃癌细胞AGS和MKN-45中运用RT-PCR和Western印迹方法检测相关基因表达;CCK8试验检测细胞增殖;划痕试验和Trans-well试验分别检测细胞迁移和侵袭。结果显示,过表达ALYREF后YAP的表达增加(P<0.05),并且促进细胞的增殖、迁移和上皮细胞-间充质转化(epithelial-mesenchymal transition, EMT)(P<0.05);靶向敲低YAP,细胞的增殖、迁移和EMT能力显著抑制(P<0.05);过表达ALYREF且靶向敲除YAP与单独过表达ALYREF相比,YAP的表达显著降低(P<0.05),且细胞的增殖、迁移和EMT能力同样得到抑制(P<0.05);靶向敲低ALYREF,细胞的增殖、克隆形成和侵袭能力显著抑制(P<0.01)。综上所述,ALYREF通过上调YAP表达进而促进胃癌细胞的增殖和迁移。 展开更多
关键词 胃癌 增殖和迁移 RNA结合蛋白Aly/ref输出因子 Yes相关蛋白
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基于ANCF的旋转超弹性厚壁REF振动特性分析
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作者 范博 王忠民 《振动与冲击》 EI CSCD 北大核心 2023年第20期245-252,共8页
为了探究子午线轮胎面内振动特性,建立了旋转超弹性厚壁REF模型。并在具有随动坐标系的绝对节点坐标法(absolute nodal coordinate formulation,ANCF)框架下,提出平面内旋转环扇单元,对旋转超弹性厚壁弹性基环模型(ring on the elastic ... 为了探究子午线轮胎面内振动特性,建立了旋转超弹性厚壁REF模型。并在具有随动坐标系的绝对节点坐标法(absolute nodal coordinate formulation,ANCF)框架下,提出平面内旋转环扇单元,对旋转超弹性厚壁弹性基环模型(ring on the elastic foundation,REF)进行离散。考虑到非线性本构关系和旋转运动中的广义惯性力,建立了该模型的非线性运动微分方程以及线性化运动微分方程。通过与现有文献中的试验结果进行对比,验证了上述模型的有效性,并分析了弹性基参数、环体厚度和旋转角速度对子午线轮胎固有特性的影响规律。 展开更多
关键词 绝对节点坐标法(ANCF) 旋转ANCF环扇单元 厚壁ref模型 超弹性材料
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晚期肺癌血清Ape1/Ref-1表达及其对阿帕替尼联合三线化疗疗效的预测价值 被引量:2
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作者 王祥 丁纪涛 刘洋 《实用癌症杂志》 2023年第9期1421-1424,共4页
目的探讨晚期肺癌血清Ape1/Ref-1的表达及其对阿帕替尼联合三线化疗疗效的预测价值。方法收集70例晚期肺癌患者病例资料。采用随机数字表法进行分组,即对照组和研究组,均35例。对照组采用常规三线化疗治疗。研究组在对照组的基础上再予... 目的探讨晚期肺癌血清Ape1/Ref-1的表达及其对阿帕替尼联合三线化疗疗效的预测价值。方法收集70例晚期肺癌患者病例资料。采用随机数字表法进行分组,即对照组和研究组,均35例。对照组采用常规三线化疗治疗。研究组在对照组的基础上再予以阿帕替尼药物治疗。结果晚期肺癌组织Ape1/Ref-1蛋白阳性表达(91.43%)显著高于癌旁组织(χ^(2)=33.600,P=0.000)。化疗前,两组晚期肺癌患者Ape1/Ref-1蛋白相对表达量比较,差异无统计学意义(P>0.05);化疗后,两组Ape1/Ref-1蛋白相对表达量均明显低于化疗前,进一步组间比较,研究组Ape1/Ref-1蛋白相对表达量明显低于对照组,差异具有统计学意义(P<0.05)。Ape1/Ref-1阴性组生存率(87.50%)显著高于Ape1/Ref-1阳性组(χ^(2)=3.949,P=0.047),研究组累积生存率(91.43%)显著高于对照组(χ^(2)=10.096,P=0.001)。结论Ape1/Ref-1在晚期肺癌组织中呈现高表达,其表达越低,阿帕替尼联合三线化疗疗效越好。 展开更多
关键词 APE1/ref-1 晚期肺癌 阿帕替尼 三线化疗 疗效预测
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非肌层浸润性膀胱癌组织中PKM2和ALYREF表达及与预后预测价值研究
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作者 朱军 沈欣 曹冬 《现代检验医学杂志》 CAS 2023年第5期110-114,154,共6页
目的 研究非肌层浸润性膀胱癌(non-muscle invasive bladder cancer,NMIBC)组织中丙酮酸激酶M2亚型(pyruvate kinase M2 subtype,PKM2),RNA结合蛋白Aly/REF输出因子(Aly/REF export factor,ALYREF)表达及预后意义。方法 选取自2017年2月... 目的 研究非肌层浸润性膀胱癌(non-muscle invasive bladder cancer,NMIBC)组织中丙酮酸激酶M2亚型(pyruvate kinase M2 subtype,PKM2),RNA结合蛋白Aly/REF输出因子(Aly/REF export factor,ALYREF)表达及预后意义。方法 选取自2017年2月~2020年2月期间南京中医药大学翰林学院附属医院收治的84例NMIBC患者为研究对象。免疫组织化学检测NMIBC和膀胱正常黏膜组织中PKM2和ALYREF蛋白表达。Spearman秩相关分析膀胱癌组织中PKM2和ALYREF蛋白表达相关性。Kaplan-Meier生存曲线分析(Log-Rank检验)PKM2和ALYREF蛋白对无进展生存(progress free survive, PFS)预后的影响。COX比例风险模型分析影响NMIBC患者PFS预后的因素。结果 NMIBC组织中PKM2(71.43%),ALYREF(73.81%)蛋白阳性率高于膀胱正常黏膜组织(11.90%,10.71%),差异具有统计学意义(χ^(2)=61.225,68.522,均P <0.05)。T1期、高级别NMIBC癌组织中PKM2(88.37%,90.32%),ALYREF(80.05%,93.55%)蛋白阳性率分别高于Ta/Tis期(53.66%,60.38%)、低级别癌组织(60.98%,62.26%),差异具有统计学意义(χ^(2)=12.393,8.594;6.524,9.903,均P <0.05)。PKM2阳性组和阴性组患者的三年总体PFS分别为48.33%(29/60),87.50%(21/24),PKM2阳性组患者三年累积PFS显著低于PKM2阴性组患者(Log-Rank testχ^(2)=10.040,P=0.002);ALYREF阳性组和阴性组三年总体PFS分别为48.39%(30/62),90.91%(20/22),ALYREF阳性组患者三年累积PFS显著低于阴性组患者(Log-Rank test χ^(2)=11.110,P=0.001)。肿瘤TNM分期T1期(HR=1.614,95%CI:1.227~2.214)、高级别(HR=1.921,95%CI:1.324~2.788),PKM2阳性(HR=1.837,95%CI:1.229~2.745),ALYREF阳性(HR=1.744,95%CI:1.245~2.443)是影响NMIBC患者PFS预后的独立危险因素。结论 NMIBC中PKM2和ALYREF表达升高,两者表达呈正相关,是影响NMIBC患者预后的独立因素。 展开更多
关键词 非肌层浸润膀胱癌 丙酮酸激酶M2亚型 RNA结合蛋白Aly/ref输出因子
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Inguinal Hernia in Adults: Diagnostic and Therapeutic Aspects at Cs Ref CI Bamako Mali
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作者 Cheickna Tounkara Amadou Maiga +9 位作者 Bessy Samake Boubacar Yoro Sidibe Tani Kone Samake Hamidou Kaloga Daye Bagayoko Oumar Amadou Malle Alhassane Traore Bakary Tientigui Dembele Pierre Adégné Togo Zanafon Ouattara 《Surgical Science》 2023年第7期507-516,共10页
The inguinal hernia is a very frequent surgical pathology in the world and it represents 95% of the hernias of the groin. This work aimed to determine the frequency of inguinal hernias, describe the clinical and thera... The inguinal hernia is a very frequent surgical pathology in the world and it represents 95% of the hernias of the groin. This work aimed to determine the frequency of inguinal hernias, describe the clinical and therapeutic aspects of inguinal hernias, describe the morbidity and mortality related to inguinal hernias and evaluate the cost of treatment. Our study was conducted from January 2010 to December 2010 in the general surgery department of the Reference Health Center of Commune I in the district of Bamako. We collected 130 cases of inguinal hernias with a sex ratio of 15.25 in favor of the male sex. The mean age was 48.02 years (standard deviation: 17.53 years). Strangulated inguinal hernias accounted for 4.9% of abdominal surgical emergencies and 14.6% of all operated inguinal hernias. More than half of the patients (82%) were operated under local anesthesia. Clinical signs were marked by painless or irreducibly painful inguinal swelling. The diagnosis was clinical in all patients. Some patients benefited from a minimum blood assessment (rhesus grouping, glycaemia, hemoglobin level and hematocrit). The Shouldice technique was performed in 68% of patients. One case of organ resection was performed, i.e. 0.8% of cases. Morbidity was 4.6% due to wall abscess;0.8% scrotal hematoma type. We have not recorded any recurrence or death. 展开更多
关键词 INGUINAL HERNIAS Surgery Cs ref CI Bamako (Mali)
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Acute Intestinal Occlusions at the Cs Ref of Commune I of Bamako
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作者 Tounkara Cheickna Samake Hamidou +7 位作者 Diarra Issaka Sanogo Modibo Traore Fousseyni Togola Modibo Traore Alhassane Dembele Bakary Tientigui Togo Pierre Adégné Kante Lassana 《Surgical Science》 2023年第12期748-757,共10页
Acute intestinal obstructions are defined as a complete and persistent cessation of materials and gases in a segment of the digestive tract. They constitute a medical-surgical emergency. Our work aimed to study acute ... Acute intestinal obstructions are defined as a complete and persistent cessation of materials and gases in a segment of the digestive tract. They constitute a medical-surgical emergency. Our work aimed to study acute intestinal obstructions, to determine the hospital frequency, to describe the aspects (epidemiological, clinical and therapeutic), to analyze the surgical consequences and to evaluate the cost of the management of obstructions. acute intestinal infections in the general surgery department of the reference health center of commune I of Bamako in Mali. Our retrospective, longitudinal and descriptive study took place from January 1, 2015 to December 31, 2019 in the general surgery department of the reference health center in commune I of Bamako. The average age was 47.72 years with extremes of 15 and 78 years and a standard deviation of 16.07. Our sex ratio (56 men/15 women) was 3.38. The clinical signs were dominated by abdominal pain (100%), vomiting (52.9%), cessation of materials and gases (25.4%) and meteorism (35.3%). The main etiologies found intraoperatively were strangulated hernia (54.9%), bands and/or adhesions (21.1%), sigmoid volvulus (12.7%), colorectal tumor (7%), small bowel volvulus (2.8%) and acute intestinal intussusception (1.4%). Hernia repair was the most performed surgical procedure, i.e. 54.9%. The overall mortality rate was 1.4%. 展开更多
关键词 Acute Intestinal Obstructions Surgery Cs ref CI BAMAKO MALI
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Acute Peritonitis at the Reference Health Center of Commune I of the District of Bamako: Epidemiological, Clinical and Therapeutic Aspects
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作者 Tounkara Cheickna Samake Hamidou +8 位作者 Maïga Amadou Cisse Alou Hamadoun Diarra Issaka Sanogo Modibo Togola Modibo Dembele Bakary Tientigui Traore Alhassane Togo Pierre Adegne Kante Lassana 《Surgical Science》 2023年第12期728-737,共10页
Acute peritonitis is most often secondary to perforation of the digestive organ and/or the spread of an intra-abdominal septic focus. The objectives of this work were to study acute peritonitis in the general surgery ... Acute peritonitis is most often secondary to perforation of the digestive organ and/or the spread of an intra-abdominal septic focus. The objectives of this work were to study acute peritonitis in the general surgery department of the Cs ref of commune I in Mali, to determine the frequency of peritonitis, to describe the epidemiological, clinical and therapeutic aspects in order to analyze the surgical consequences and to assess the additional cost of treating acute peritonitis. This study was prospective, descriptive, cross-sectional involving 40 patients received in the surgery department of the Cs ref of commune I for acute peritonitis from January 1, 2018 to December 31, 2018. There were 40 patients among whom 28 (70%) were men and 12 were women (30%), i.e. a sex ratio = 2.3. The average age was 25 years with extremes varying between 16 and 54 years and a standard deviation of 11.78. Abdominal pain was the main reason for consultation. Clinical examination alone made it possible to make the diagnosis in 75% of cases. Surgical treatment depended on the intraoperative etiology. The clinical diagnosis was supported by ASP and abdominal ultrasound;performed respectively in 10% and 90% of patients. Appendiceal peritonitis was the intraoperative diagnosis observed in 50% of cases. All our patients benefited from a peritoneal toilet with drainage. We noted a morbidity rate of 5% dominated by parietal suppuration. The average cost of care was 175,000 FCFA. 展开更多
关键词 Acute Peritonitis Epidemiology Diagnostic Therapy Surgical Emergencies Cs ref CI BAMAKO MALI
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氧化/还原因子ref-1在肺癌组织中的细胞定位表达及与8-OH-dG的关系 被引量:10
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作者 吕嘉春 何敏 +6 位作者 廖永德 王孝养 黎银燕 曾波航 陈家堃 吴中亮 施侣元 《肿瘤》 CAS CSCD 北大核心 2004年第1期35-37,共3页
目的 氧化 /还原因子 1(ref 1) ,也称为AP核酸内切酶 (APE) ,是氧化还原和DNA碱基切除修复途径中的重要一员。本研究旨在探讨ref 1在肺癌中的表达及其与氧化损伤标志 8 羟基 脱氧鸟苷 (8 OH dG)的关系。方法 用免疫组化法检测15 0例... 目的 氧化 /还原因子 1(ref 1) ,也称为AP核酸内切酶 (APE) ,是氧化还原和DNA碱基切除修复途径中的重要一员。本研究旨在探讨ref 1在肺癌中的表达及其与氧化损伤标志 8 羟基 脱氧鸟苷 (8 OH dG)的关系。方法 用免疫组化法检测15 0例肺癌组织、12 0例癌旁肺组织、4 0例肺良性病变和 4 0例正常肺组织中氧化 /还原因子ref 1基因的蛋白质表达 ;分析有关暴露因素对ref 1表达的影响 ,探讨ref 1基因与 8 OH dG的关系。结果 肺癌和正常肺组织皆有ref 1的表达 ,但ref 1在细胞内的定位有所不同 ,70 .0 % (10 5 / 15 0 )的肺癌组织ref 1从通常的胞核定位移位到胞浆 ,仅 7.5 % (3/ 4 0 )的正常肺组织有ref 1移位胞浆的现象 ,两者差异显著 ,P <0 .0 1。未发现肺癌患者的性别、年龄、吸烟和肿瘤家族史等因素与ref 1细胞定位之间有联系。ref 1移位胞浆与肺组织中 8 OH dG的含量呈显著正相关 ,Spearman相关系数为 0 .70 2 ,P <0 .0 1。结论 ref 1的细胞定位对其氧化 /还原和修复功能有着重要影响 ,可作为监测细胞DNA修复功能的指标。 展开更多
关键词 氧化/还原因子 ref-1 肺癌 细胞定位表达 与8-OH-dG 癌组织
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应用酵母双杂交系统筛选橡胶延长因子REF的互作蛋白 被引量:7
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作者 曾日中 李先昆 +3 位作者 聂智毅 代龙军 黎瑜 段翠芳 《热带亚热带植物学报》 CAS CSCD 北大核心 2011年第5期400-406,共7页
利用酵母双杂交系统,以橡胶树(Hevea brasiliensis)橡胶延长因子基因REF的开放阅读框(ORF)构建无自激活性的诱饵表达载体pBD-GAL4-REF,并筛选以pAD-GAL4-2.1载体构建的橡胶树胶乳cDNA文库,对阳性克隆的cDNA插入片段进行测序及生物学功... 利用酵母双杂交系统,以橡胶树(Hevea brasiliensis)橡胶延长因子基因REF的开放阅读框(ORF)构建无自激活性的诱饵表达载体pBD-GAL4-REF,并筛选以pAD-GAL4-2.1载体构建的橡胶树胶乳cDNA文库,对阳性克隆的cDNA插入片段进行测序及生物学功能分析。通过酵母双杂交筛选,共获得5种可能与REF互作的候选蛋白质,它们分别为与诱饵蛋白REF高度同源的REF家族成员、小橡胶粒子蛋白(SRPP)、翻译控制肿瘤蛋白(TCTP)、激发子响应蛋白和泛素耦联酶E2,这表明橡胶延长因子REF除了与自身高度同源蛋白质可能存在相互作用之外,还可能与TCTP和激发子响应蛋白等其它蛋白质发生相互作用。这些结果有助于揭示橡胶粒子的生物学功能。 展开更多
关键词 橡胶树 橡胶延长因子ref 橡胶生物合成 酵母双杂交系统
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吉西他滨对人胰腺癌Patu-8988细胞株APE/Ref-1的诱导作用 被引量:8
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作者 熊光苏 吴叔明 +4 位作者 徐晓晶 周鋆 朱红音 李恩灵 莫剑忠 《世界华人消化杂志》 CAS 北大核心 2007年第12期1425-1428,共4页
目的:研究人胰腺癌细胞株在吉西他滨化疗时APE/Ref-1基因表达的变化,并试图揭示其在胰腺癌化疗耐药中所起的作用.方法:不同浓度吉西他滨0、10、20、40及60μmol/L作用人胰腺癌Patu-8988细胞株24 h,分别以RT-PCR及Western blot方法测定... 目的:研究人胰腺癌细胞株在吉西他滨化疗时APE/Ref-1基因表达的变化,并试图揭示其在胰腺癌化疗耐药中所起的作用.方法:不同浓度吉西他滨0、10、20、40及60μmol/L作用人胰腺癌Patu-8988细胞株24 h,分别以RT-PCR及Western blot方法测定作用后APE/Ref-1的mRNA及蛋白表达情况.结果:吉西他滨作用人胰腺癌Patu-8988细胞株24h后,APE/Ref-1的mRNA及蛋白表达水平明显上升,并与吉西他滨的浓度呈正相关(RT-PCR:r=0.645,P=0.012;Western blot:r= 0.598,P=0.020).结论:APE/Ref-1在胰腺癌化疗时表达明显增强,可能与化疗耐药性的产生有关,并提示针对APE/Ref-1的靶向干预可能有助于提高胰腺癌的化疗敏感性. 展开更多
关键词 胰腺肿瘤 无嘌呤嘧啶核酸内切酶 吉西他滨 逆转录-聚合酶链反应 免疫印迹
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乙型脑炎病毒核酸检测试剂国家参考品的研制
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作者 夏德菊 周海卫 +1 位作者 刘东来 许四宏 《分子诊断与治疗杂志》 2024年第5期796-799,803,共5页
目的建立乙型脑炎病毒核酸检测试剂国家参考品,确定其质量标准。方法通过对24份病原体培养物的复核、确证,全基因组测序及基因分型,组成乙型脑炎病毒核酸检测试剂国家参考品,经多家实验室的协助标定,确定参考品的质量标准,并对参考品进... 目的建立乙型脑炎病毒核酸检测试剂国家参考品,确定其质量标准。方法通过对24份病原体培养物的复核、确证,全基因组测序及基因分型,组成乙型脑炎病毒核酸检测试剂国家参考品,经多家实验室的协助标定,确定参考品的质量标准,并对参考品进行均匀性及稳定性评估。结果乙型脑炎病毒核酸检测试剂国家参考品由10份阴性、14份阳性、1份最低检出限和1份精密度样品组成。质量标准为10份阴性参考品应均检出阴性;14份阳性参考品应至少检出13份阳性;精密度考品重复检测10次,要求均为乙型脑炎病毒阳性,且其检测值的CV应不大于5.0%;最低检出限参考品要求1∶102倍稀释及以上浓度时应为乙型脑炎病毒阳性。参考品的均匀性及稳定性均符合要求。结论该参考品可用作乙型脑炎病毒核酸检测试剂的质量控制及评价。 展开更多
关键词 乙型脑炎病毒 核酸检测试剂 基因分型 国家参考品
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姜黄素对缺血/再灌注大鼠APE/Ref-1表达及细胞凋亡的影响 被引量:3
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作者 王智 薛荣亮 +2 位作者 赵红霞 高慧 魏欣 《山东医药》 CAS 2013年第37期1-3,11,共4页
目的观察姜黄素对大鼠全脑缺血/再灌注损伤后海马神经元凋亡与DNA修复蛋白APE/Ref-1表达的影响,探讨其对脑缺血损伤后的保护作用。方法将144只雄性SD大鼠随机平均分为假手术组(SO组)、缺血/再灌注组(IR组)和姜黄素干预组(CU组)各48只。... 目的观察姜黄素对大鼠全脑缺血/再灌注损伤后海马神经元凋亡与DNA修复蛋白APE/Ref-1表达的影响,探讨其对脑缺血损伤后的保护作用。方法将144只雄性SD大鼠随机平均分为假手术组(SO组)、缺血/再灌注组(IR组)和姜黄素干预组(CU组)各48只。建立大鼠全脑缺血/再灌注模型,CU组于再灌注后即刻经腹腔注射姜黄素。分别于再灌注后2、6、12、24、48、72 h处死大鼠,提取大鼠脑组织。原位末端标记法检测海马CA1区的细胞凋亡情况;免疫组化SABC法检测APE/Ref-1的表达。结果 SO组神经元细胞凋亡较少,IR组凋亡的神经元细胞于再灌注6 h开始增多,48 h细胞凋亡率达到最高。与SO组比较,IR组细胞凋亡率增高(P<0.01)。CU组在再灌注6 h后的各时点神经元细胞凋亡较IR组少(P均<0.01)。SO组APE/Ref-1阳性细胞在各时点较多;IR组于再灌注后2 h APE/Ref-1阳性细胞开始出现明显减少,一直持续至72 h,与SO组比较有统计学差异(P均<0.01);CU组APE/Ref-1阳性细胞率各时点与IR组比较均有统计学差异(P均<0.01)。结论姜黄素发挥脑保护作用的机制可能与减缓DNA修复蛋白APE/Ref-1表达的下降和阻止脑缺血/再灌注损伤区神经元细胞的凋亡有关。 展开更多
关键词 再灌注损伤 APE ref-1 姜黄素 大鼠
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杜仲Ref基因密码子偏好性分析 被引量:7
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作者 刘慧敏 乌云塔娜 杜红岩 《中南林业科技大学学报》 CAS CSCD 北大核心 2016年第3期8-12,共5页
杜仲是世界上唯一的硬橡胶树种,是能够替代三叶橡胶的最具开发潜力的胶源植物。本研究以杜仲胶合成下游途径Ref基因为研究对象,运用Codon W和CUSPS等研究Ref基因的密码子组成特点及使用偏好性。研究结果表明,杜仲Ref基因的GC含量为49%,... 杜仲是世界上唯一的硬橡胶树种,是能够替代三叶橡胶的最具开发潜力的胶源植物。本研究以杜仲胶合成下游途径Ref基因为研究对象,运用Codon W和CUSPS等研究Ref基因的密码子组成特点及使用偏好性。研究结果表明,杜仲Ref基因的GC含量为49%,密码子第三位碱基的GC含量为59%;杜仲Ref基因有28个偏好使用的密码子,其中约60%(17个)的密码子以G/C结尾;与大肠杆菌、酵母、拟南芥和烟草的基因组密码子使用偏好性比较,杜仲与拟南芥基因组密码子的使用偏性更为接近,拟南芥更适合作为Ref转基因的受体。本研究为预测杜仲Ref基因的异源表达,适宜表达系统的选择以及Ref基因密码子的改造以提高其在宿主中的表达,进行有效的基因功能验证提供理论参考。 展开更多
关键词 杜仲 ref基因 密码子 偏好性
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红景天苷对脑缺血再灌注大鼠脑神经元APE/Ref-1的影响 被引量:10
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作者 刘晓梅 王淑英 刘斌 《中国老年学杂志》 CAS CSCD 北大核心 2009年第5期518-520,共3页
目的研究红景天苷对局灶性脑缺血/再灌注(I/R)损伤后神经可塑性和DNA修复酶APE/Ref表达的影响。方法Wistar大鼠随机分为假手术组,I/R模型组和红景天苷干预组,采用线栓法制造大鼠大脑局灶缺血(2h)/再灌注模型,于灌注后0.25、1、3、7d4个... 目的研究红景天苷对局灶性脑缺血/再灌注(I/R)损伤后神经可塑性和DNA修复酶APE/Ref表达的影响。方法Wistar大鼠随机分为假手术组,I/R模型组和红景天苷干预组,采用线栓法制造大鼠大脑局灶缺血(2h)/再灌注模型,于灌注后0.25、1、3、7d4个时间点断头取脑,HE和尼氏染色观察神经元形态学变化,免疫组化方法检测无嘌呤无嘧啶位点/氧化还原因子-1(APE/Ref-1)的表达。结果与其他两组比较,红景天苷组梗死灶范围明显减小,梗死灶周围皮质神经元损伤明显减轻(P<0.05);APE/Ref-1阳性细胞数表达显著增高(P<0.05)。结论红景天可以易化脑I/R损伤后中枢神经系统神经再生和修复的可塑性,抑制APE/Ref-1降低可能是其在I/R早期发挥神经保护作用的一条重要途径。 展开更多
关键词 红景天苷 脑缺血/再灌注 神经可塑性 APE/ref
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PC12细胞APE/ref-1cDNA的克隆和表达 被引量:3
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作者 谢振华 王爱民 +1 位作者 刘长振 马春 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2001年第5期585-589,共5页
APE Ref 1是双功能核蛋白 ,它既能在碱基切除修复过程中切除脱嘌呤 脱嘌啶位点 ,又能促进包括AP 1、Myb和NF κB等受氧化还原调节的转录因子对DNA的结合 .从PC12细胞中抽提总RNA ,经逆转录PCR(RT PCR)扩增出APE ref 1cDNA并克隆到pQE3 ... APE Ref 1是双功能核蛋白 ,它既能在碱基切除修复过程中切除脱嘌呤 脱嘌啶位点 ,又能促进包括AP 1、Myb和NF κB等受氧化还原调节的转录因子对DNA的结合 .从PC12细胞中抽提总RNA ,经逆转录PCR(RT PCR)扩增出APE ref 1cDNA并克隆到pQE3 1表达质粒上的BamHⅠ和PstⅠ位点间 .经测序表明 ,PC12细胞的APE ref 1cDNA以正确的阅读框架重组进入表达质粒 ,表达重组质粒pQE3 1 APE在宿主菌BL2 1中得到稳定表达 .SDS PAGE鉴定表明 ,带 6个组氨酸的融合蛋白分子量为 3 8kD ,并经Ni NTA琼脂糖亲和纯化得到电泳纯融合蛋白 .Western印迹证明重组蛋白为APE ref 1融合蛋白 . 展开更多
关键词 PC12细胞 APE/ref-1 RT-PCR 克隆 表达 限速酶
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