On the basis of the vector diffraction theory and Green's function method, this paper investigates the effects of refractive index mismatch on second-harmonic generation (SHG) microscopy. The polarization distribut...On the basis of the vector diffraction theory and Green's function method, this paper investigates the effects of refractive index mismatch on second-harmonic generation (SHG) microscopy. The polarization distribution and SHG intensity are calculated as functions of the sample radius and probe depth. The numerical results show that refractive index mismatch can result in peak intensity degradation, increase secondary lobes and extension of second- harmonic polarization distribution. Because of the attenuation of polarization intensity, the detected SHG intensity significantly decreases with increasing probe depth, which can limit the imaging depth of SHG microscopy inside thick samples. Forward SHG intensity decays slowly than backward SHG, due to the combination of extension second-harmonic polarization distribution and strong dependency of forward SHG on sample radius.展开更多
The complex amplitude distribution which can compensate for the spherical aberration caused by mismatch on lnterthce between recording material and immersion material (air) on three-dimensional optical storage syste...The complex amplitude distribution which can compensate for the spherical aberration caused by mismatch on lnterthce between recording material and immersion material (air) on three-dimensional optical storage system is derived analytically and numerically. Comparison between two approaches is made. It is also shown how the depth of recording layer and position of reference plane are related to the phase distribution at reference plane and how the mismatch can be resolved by using a pure phase element.展开更多
文摘On the basis of the vector diffraction theory and Green's function method, this paper investigates the effects of refractive index mismatch on second-harmonic generation (SHG) microscopy. The polarization distribution and SHG intensity are calculated as functions of the sample radius and probe depth. The numerical results show that refractive index mismatch can result in peak intensity degradation, increase secondary lobes and extension of second- harmonic polarization distribution. Because of the attenuation of polarization intensity, the detected SHG intensity significantly decreases with increasing probe depth, which can limit the imaging depth of SHG microscopy inside thick samples. Forward SHG intensity decays slowly than backward SHG, due to the combination of extension second-harmonic polarization distribution and strong dependency of forward SHG on sample radius.
文摘The complex amplitude distribution which can compensate for the spherical aberration caused by mismatch on lnterthce between recording material and immersion material (air) on three-dimensional optical storage system is derived analytically and numerically. Comparison between two approaches is made. It is also shown how the depth of recording layer and position of reference plane are related to the phase distribution at reference plane and how the mismatch can be resolved by using a pure phase element.