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An improved approach to H-two control withregional stability constraints
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作者 Yanjun SHEN Tingsong DU 《控制理论与应用(英文版)》 EI 2007年第4期380-384,共5页
This paper presents an improved method for H-two control with regional stability constraints for the closedloop system. The controller is given by the convex combination of a set of fixed gains. Both continuous and di... This paper presents an improved method for H-two control with regional stability constraints for the closedloop system. The controller is given by the convex combination of a set of fixed gains. Both continuous and discrete-time systems in a known polytopic domain are investigated. New LMI-based sufficient conditions for the existence of parameterdependent Lyapunov functions are proposed. Numerical examples are given to show the proposed conditions provide useful and less conservative results for the problems of H-two control with regional stability constraints. 展开更多
关键词 H2 control regional stability constraints Polytopic domains LMI
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The Static Stability Region of an Integrated Electricity-Gas System Considering Voltage and Gas Pressure
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作者 Yunfei Mu Zhibin Liu +5 位作者 Xiangwei Guo Hongjie Jia Kai Hou Xiaodan Yu Bofeng Luo Hairun Li 《Engineering》 SCIE EI CAS CSCD 2024年第5期132-145,共14页
In an integrated electricity-gas system(IEGS),load fluctuations affect not only the voltage in the power system but also the gas pressure in the natural gas system.The static voltage stability region(SVSR)method is a ... In an integrated electricity-gas system(IEGS),load fluctuations affect not only the voltage in the power system but also the gas pressure in the natural gas system.The static voltage stability region(SVSR)method is a tool for analyzing the overall static voltage stability in a power system.However,in an IEGS,the SVSR boundary may be overly optimistic because the gas pressure may collapse before the voltage collapses.Thus,the SVSR method cannot be directly applied to an IEGS.In this paper,the concept of the SVSR is extended to the IEGS-static stability region(SSR)while considering voltage and gas pressure.First,criteria for static gas pressure stability in a natural gas system are proposed,based on the static voltage stability criteria in a power system.Then,the IEGS-SSR is defined as a set of active power injections that satisfies multi-energy flow(MEF)equations and static voltage and gas pressure stability constraints in the active power injection space of natural gas-fired generator units(NGUs).To determine the IEGSSSR,a continuation MEF(CMEF)method is employed to trace the boundary point in one specific NGU scheduling direction.A multidimensional hyperplane sampling method is also proposed to sample the NGU scheduling directions evenly.The obtained boundary points are further used to form the IEGSSSR in three-dimensional(3D)space via a Delaunay triangulation hypersurface fitting method.Finally,the numerical results of typical case studies are presented to demonstrate that the proposed method can effectively form the IEGS-SSR,providing a tool for IEGS online monitoring and dispatching. 展开更多
关键词 Integrated electricity-gas system Static stability region Continuation multi-energy flow Multidimensional hyperplane sampling
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Regional Stability of Positive Switched Linear Systems with Multi-equilibrium Points 被引量:2
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作者 Zhi Liu Yu-Zhen Wang 《International Journal of Automation and computing》 EI CSCD 2017年第2期213-220,共8页
This paper studies the regional stability for positive switched linear systems with multi-equilibrium points (PSLS-MEP). First, a sufficient condition is presented for the regional stability of PSLS-MEP via a common... This paper studies the regional stability for positive switched linear systems with multi-equilibrium points (PSLS-MEP). First, a sufficient condition is presented for the regional stability of PSLS-MEP via a common linear Lyapunov function. Second, by establishing multiple Lyapunov functions, a dwell time based condition is proposed for the regional stability analysis. Third, a suprasphere which contains all equilibrium points is constructed as a stability region of the considered PSLS-MEP, which is less conservative than existing results. Finally, the study of an illustrative example shows that the obtained results are effective in the regional stability analysis of PSLS-MEP. 展开更多
关键词 regional stability positive switched linear system multi-equilibrium points common linear Lyapunov function multipleLyapunov function.
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Quantitative Assessment and Zonation of Regional Crustal Stability in China
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作者 Sun Ye Tan Chengxuan(Urban and Engineering Site Stability Research Center, CAGS, Beijing 101601) 《Journal of Earth Science》 SCIE CAS CSCD 1996年第2期209-215,共7页
On the basis of the synthetic analysis of present regional tectonic stress field in China, this paper follows three steps to do crustal stability assessment: (1) zonation of preevaluation area for regional crustal sta... On the basis of the synthetic analysis of present regional tectonic stress field in China, this paper follows three steps to do crustal stability assessment: (1) zonation of preevaluation area for regional crustal stability assessment and zonation; (2) choice, taking value and weight distribution of quantitative evaluation indices and determination of evaluation standards; (3) assessment and zonation of regional crustal stability using fuzzy mathematics in China. 展开更多
关键词 regional crustal stability tectonic stress field quantitative assessment
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Engineering Geological Study of Regional Tectonic Stability in the Area of the Longyang Gorge Power Station on the Huanghe (Yellow) River
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作者 Wang Shitian, Li Yusheng, Su Daogang and Wei Lunwu Chengdu College of Geology, Chengdu, Sichuan Fei Zhenbi 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 1989年第2期197-209,共13页
In the stability study of the regional structures in the area of the Longyang Gorge Hydroelectrical Power Station, a model of the current stress-deformation field of the area was constructed based on analyses of avail... In the stability study of the regional structures in the area of the Longyang Gorge Hydroelectrical Power Station, a model of the current stress-deformation field of the area was constructed based on analyses of available data of regional surveys and historical earthquakes and field investigations of active faults and ancient earthquakes. This model was examined and verified by physical and mathematical simulation experiments, and quantitative relations and data were obtained. 展开更多
关键词 RIVER area Engineering Geological Study of regional Tectonic stability in the Area of the Longyang Gorge Power Station on the Huanghe YELLOW
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Seismic Activity and Fuzzy Comprehensive Evaluation of Crustal Stability in the Beijing Region
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作者 Huang Qinghua, Ma Yinsheng, Institute of Geomechanics, C hinese Academy of Geological Science, Beijingand Song Xinchu Seismological Bureau of Zhejiang Province, Hangzhou, Zhejiang 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 1994年第1期1-13,共13页
This paper discusses the seismic activity in Beijing M unicipality and its adjacent areas (which are referred to as the Beijing region in the paper). By using fuzzy mathematics, the factors controlling regional crusta... This paper discusses the seismic activity in Beijing M unicipality and its adjacent areas (which are referred to as the Beijing region in the paper). By using fuzzy mathematics, the factors controlling regional crustal stability are transformed into fuzzy information and then a model of fuzzy diagnosis is established. A fuzzy comprehensive evaluation of regional crustal stability in the Beijing region is made according to the seismic and geological conditions of the region. 展开更多
关键词 fuzzy mathematics regional stability BEIJING
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Model Predictive Control of Nonlinear Systems: Stability Region and Feasible Initial Control 被引量:5
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作者 Xiao-Bing Hu Wen-Hua Chen 《International Journal of Automation and computing》 EI 2007年第2期195-202,共8页
This paper proposes a new method for model predictive control (MPC) of nonlinear systems to calculate stability region and feasible initial control profile/sequence, which are important to the implementations of MPC... This paper proposes a new method for model predictive control (MPC) of nonlinear systems to calculate stability region and feasible initial control profile/sequence, which are important to the implementations of MPC. Different from many existing methods, this paper distinguishes stability region from conservative terminal region. With global linearization, linear differential inclusion (LDI) and linear matrix inequality (LMI) techniques, a nonlinear system is transformed into a convex set of linear systems, and then the vertices of the set are used off-line to design the controller, to estimate stability region, and also to determine a feasible initial control profile/sequence. The advantages of the proposed method are demonstrated by simulation study. 展开更多
关键词 Model predictive control (MPC) stability region terminal region linear differential inclusion (LDI) linear matrix inequality (LMI).
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Real-time digital image stabilization based on regional field image gray projection 被引量:4
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作者 Weiping Yang Zhilong Zhang +3 位作者 Yan Zhang Xinping Lu Jicheng Li Zhiguang Shi 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2016年第1期224-231,共8页
Digital image stabilization technique plays important roles in video surveillance and object acquisition.Many useful electronic image stabilization algorithms have been studied.A real-time algorithm is proposed based ... Digital image stabilization technique plays important roles in video surveillance and object acquisition.Many useful electronic image stabilization algorithms have been studied.A real-time algorithm is proposed based on field image gray projection which enables the regional odd and even field image to be projected into x and y directions and thus to get the regional gray projection curves in x and y directions,respectively.For the odd field image channel,motion parameters can be estimated via iterative minimum absolute difference based on two successive field image regional gray projection curves.Then motion compensations can be obtained after using the Kalman filter method.Finally,the odd field image is adjusted according to the compensations.In the mean time,motion compensation is applied to the even field image channel with the odd field image gray projection curves of the current frame.By minimizing absolute difference between odd and even field image gray projection curves of the current frame,the inter-field motion parameters can be estimated.Therefore,the even field image can be adjusted by combining the inter-field motion parameters and the odd field compensations.Finally,the stabilized image sequence can be obtained by synthesizing the adjusted odd and even field images.Experimental results show that the proposed algorithm can run in real-time and have a good stabilization performance.In addition,image blurring can be improved. 展开更多
关键词 image dithering field based processing electronic image stabilization regional gray projection minimum absolute difference.
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Closed-loop control of epileptiform activities in a neural population model using a proportional-derivative controller 被引量:4
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作者 王俊松 王美丽 +1 位作者 李小俚 Ernst Niebur 《Chinese Physics B》 SCIE EI CAS CSCD 2015年第3期434-441,共8页
Epilepsy is believed to be caused by a lack of balance between excitation and inhibitation in the brain. A promising strategy for the control of the disease is closed-loop brain stimulation. How to determine the stimu... Epilepsy is believed to be caused by a lack of balance between excitation and inhibitation in the brain. A promising strategy for the control of the disease is closed-loop brain stimulation. How to determine the stimulation control parameters for effective and safe treatment protocols remains, however, an unsolved question. To constrain the complex dynamics of the biological brain, we use a neural population model(NPM). We propose that a proportional-derivative(PD) type closed-loop control can successfully suppress epileptiform activities. First, we determine the stability of root loci, which reveals that the dynamical mechanism underlying epilepsy in the NPM is the loss of homeostatic control caused by the lack of balance between excitation and inhibition. Then, we design a PD type closed-loop controller to stabilize the unstable NPM such that the homeostatic equilibriums are maintained; we show that epileptiform activities are successfully suppressed. A graphical approach is employed to determine the stabilizing region of the PD controller in the parameter space, providing a theoretical guideline for the selection of the PD control parameters. Furthermore, we establish the relationship between the control parameters and the model parameters in the form of stabilizing regions to help understand the mechanism of suppressing epileptiform activities in the NPM. Simulations show that the PD-type closed-loop control strategy can effectively suppress epileptiform activities in the NPM. 展开更多
关键词 neural population model epileptiform activities proportional-derivative controller stabilizing region
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Main active faults and seismic activity along the Yangtze River Economic Belt:Based on remote sensing geological survey 被引量:7
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作者 Zhong-hai Wu Chun-jing Zhou +2 位作者 Xiao-long Huang Gen-mo Zhao Cheng-xuan Tan 《China Geology》 2020年第2期314-338,共25页
The Yangtze River Economic Belt(YREB)spans three terrain steps in China and features diverse topography that is characterized by significant differences in geological structure and presentday crustal deformation.Activ... The Yangtze River Economic Belt(YREB)spans three terrain steps in China and features diverse topography that is characterized by significant differences in geological structure and presentday crustal deformation.Active faults and seismic activity are important geological factors for the planning and development of the YREB.In this paper,the spatial distribution and activity of 165 active faults that exist along the YREB have been compiled from previous findings,using both remote-sensing data and geological survey results.The crustal stability of seven particularly noteworthy typical active fault zones and their potential effects on the crustal stability of the urban agglomerations are analyzed.The main active fault zones in the western YREB,together with the neighboring regional active faults,make up an arc fault block region comprising primarily of Sichuan-Yunnan and a“Sichuan-Yunnan arc rotational-shear active tectonic system”strong deformation region that features rotation,shear and extensional deformation.The active faults in the central-eastern YREB,with seven NE-NNE and seven NW-NWW active faults(the“7-longitudinal,7-horizontal”pattern),macroscopically make up a“chessboard tectonic system”medium-weak deformation region in the geomechanical tectonic system.They are also the main geological constraints for the crustal stability of the YREB. 展开更多
关键词 Active faults and tectonic system Paleo-earthquake regional crustal stability Seismic risk assessment Yangtze River China
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A Modified Model Predictive Control Scheme 被引量:1
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作者 Xiao-Bing Hu Wen-Hua Chen 《International Journal of Automation and computing》 EI 2005年第1期101-106,共6页
In implementations of MPC (Model Predictive Control) schemes, two issues need to be addressed. One is how to enlarge the stability region as much as possible. The other is how to guarantee stability when a computation... In implementations of MPC (Model Predictive Control) schemes, two issues need to be addressed. One is how to enlarge the stability region as much as possible. The other is how to guarantee stability when a computational time limitation exists. In this paper, a modified MPC scheme for constrained linear systems is described. An offline LMI-based iteration process is introduced to expand the stability region. At the same time, a database of feasible control sequences is generated offline so that stability can still be guaranteed in the case of computational time limitations. Simulation results illustrate the effectiveness of this new approach. Keywords Model predictive control - linear matrix inequality - stability region - terminal region - feasibility Xiao-Bing Hu received the B.S. degree in Aviation Electronic Engineering at Civil Aviation Institute of China, Tianjin, China, in 1998, the M.S. degree in Automatic Control Engineering at Nanjing University of Aeronautics & Astronautics, Nanjing, China, in 2001, and the Ph.D. degree in Aeronautical and Automotive Engineering at Loughborough University, UK, in 2005.He is currently a Research Fellow in Department of Informatics at Sussex University, UK. His major field of research includes predictive control, artificial intelligence, air traffic management, and flight control. Wen-Hua Chen received his MSc and Ph.D. degrees from Department of Automatic Control at Northeast University, China, in 1989 and 1991, respectively.From 1991 to 1996, he was a Lecturer in Department of Automatic Control at Nanjing University of Aeronautics & Astronautics, China. He held a research position and then a Lectureship in Control Engineering in Center for Systems and Control at University of Glasgow, UK, from 1997 to 2000. He holds a Lectureship in Flight Control Systems in Department of Aeronautical and Automotive Engineering at Loughborough University, UK. He has published one book and more than 60 papers on journals and conferences. His research interests are the development of advanced control strategies and their applications in aerospace engineering. 展开更多
关键词 Model predictive control linear matrix inequality stability region terminal region FEASIBILITY
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Weak and strong stabilization of linear systems
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作者 Mohamed OUZAHRA Hamid BOURRAY +1 位作者 Asmae KAMAL Rachid El AYADI 《控制理论与应用(英文版)》 EI 2009年第4期359-366,共8页
We study the problem of stabilizing a distributed linear system on a subregion of its geometrical domain. We are concerned with two methods: the first approach enables us to characterize a stabilizing control via the... We study the problem of stabilizing a distributed linear system on a subregion of its geometrical domain. We are concerned with two methods: the first approach enables us to characterize a stabilizing control via the steady state Riccati equation, and the second one is based on decomposing the state space into two suitable subspaces and studying the projections of the initial system onto such subspaces. The obtained results are performed through various examples. 展开更多
关键词 Linear systems regional stabilization Riccati equation Decomposed method
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On Stabilizing Sets of PI Controllers for Multiple Time Delayed Process
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作者 郑达 任正云 方建安 《Journal of Donghua University(English Edition)》 EI CAS 2009年第1期36-39,共4页
This paper considers the problem of stabilizing multiple time delayed processes using proportional integral(PI) controller.The presented approach is based on finding all possible values of control parameters which wil... This paper considers the problem of stabilizing multiple time delayed processes using proportional integral(PI) controller.The presented approach is based on finding all possible values of control parameters which will result in pure imaginary roots of closed loop characteristic equation under all process parameters fixed.The ergodic search of three PI control parameters are converted from the range of infinity to finite range by introducing trigonometric tangent function.After all possible stability boundaries are obtained,the Nyquist stability method is used to determine the actual stability region of the controller parameters.This method also permits design for simultaneous minimum gain and phase margin requirement.An illustrative example case is also presented. 展开更多
关键词 proportional-integral controller multiple timedelay robust stability stability region
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Optimal Robust Control for Unstable Delay System
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作者 Rihem Farkh Khaled A.Aljaloud +1 位作者 Moufida Ksouri Faouzi Bouani 《Computer Systems Science & Engineering》 SCIE EI 2021年第2期307-321,共15页
Proportional-Integral-Derivative control system has been widely used in industrial applications.For uncertain and unstable systems,tuning controller parameters to satisfy the process requirements is very challenging.I... Proportional-Integral-Derivative control system has been widely used in industrial applications.For uncertain and unstable systems,tuning controller parameters to satisfy the process requirements is very challenging.In general,the whole system’s performance strongly depends on the controller’s efficiency and hence the tuning process plays a key role in the system’s response.This paper presents a robust optimal Proportional-Integral-Derivative controller design methodology for the control of unstable delay system with parametric uncertainty using a combination of Kharitonov theorem and genetic algorithm optimization based approaches.In this study,the Generalized Kharitonov Theorem(GKT)for quasi-polynomials is employed for the purpose of designing a robust controller that can simultaneously stabilize a given unstable second-order interval plant family with time delay.Using a constructive procedure based on the Hermite-Biehler theorem,we obtain all the Proportional-Integral-Derivative gains that stabilize the uncertain and unstable second-order delay system.Genetic Algorithms(GAs)are utilized to optimize the three parameters of the PID controllers and the three parameters of the system which provide the best control that makes the system robust stable under uncertainties.Specifically,the method uses genetic algorithms to determine the optimum parameters by minimizing the integral of time-weighted absolute error ITAE,the Integral-Square-Error ISE,the integral of absolute error IAE and the integral of time-weighted Square-Error ITSE.The validity and relatively effortless application of presented theoretical concepts are demonstrated through a computation and simulation example. 展开更多
关键词 Unstable time-delay system interval plants generalized Kharitonov theorem PID controller Hermite-Biehler theorem stability region genetic algorithms optimum PID controller optimum system parameters
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On expansion of estimated stability region:Theory,methodology,and application to power systems 被引量:9
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作者 LIU Feng WEI Wei MEI ShengWei 《Science China(Technological Sciences)》 SCIE EI CAS 2011年第6期1394-1406,共13页
The expansion of the estimated stability region plays an important role in the stability analysis of nonlinear systems.However,current literatures have not provided a complete mathematical description for this problem... The expansion of the estimated stability region plays an important role in the stability analysis of nonlinear systems.However,current literatures have not provided a complete mathematical description for this problem.This paper reveals that essentially the enlargement or the compression of the estimated stability region results directly from the diffeomorphism map,which is induced by the flow contained in the stability region.By proving that any integration algorithm with an order higher than one can approximately trace the flow of the system,a generalized methodology is proposed to construct various algorithms to realize the enlargement or the compression of the estimated stability region.With this methodology,two new algorithms based on symbolic calculation are suggested to reduce the computational burden.Furthermore,this methodology is applied to construct a scalable numerical algorithm to calculate the critical clearing time(CCT) of the power system for given faults.Tests on the IEEE 10-machine 39-bus system show that the computational results coincide well with the step-by-step simulation with high accuracy. 展开更多
关键词 nonlinear system stability region power systems transient stability analysis
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Lateral stability region conservativeness estimation and torque distribution for FWIA electric vehicle steering 被引量:5
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作者 YIN Guo Dong JIN Xian Jian +1 位作者 QING Zhi Yong BIAN Chen Tong 《Science China(Technological Sciences)》 SCIE EI CAS CSCD 2015年第4期669-676,共8页
Estimation of the lateral stability region and torque distribution on steering is very important to improve stability in lateral handling for all wheel drive electric vehicles.Based on the built-nonlinear vehicle dyna... Estimation of the lateral stability region and torque distribution on steering is very important to improve stability in lateral handling for all wheel drive electric vehicles.Based on the built-nonlinear vehicle dynamic model,the lateral stability region of the vehicle related to steering is estimated using Lyapunov function.We obtained stable equilibrium points of non-straight driving according to the estimated lateral stability region and also reconstructed the Lyapunov function matrix,which proved that the closed-loop system composed of yaw rate and lateral velocity is satisfied with negative definite property.In addition,the designed controller dynamically allocates the drive torque in terms of the vertical load and slip rate of the four wheels.The simulation results show that the estimated lateral stability region and the designed controller are satisfactory in handling stability performance against different roads and vehicle parameters. 展开更多
关键词 four-wheel independently actuated electric vehicle torque distribution vehicle steering stability region estimation
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Small signal stability region of power systems with DFIG in injection space 被引量:5
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作者 Chao QIN Yixin YU 《Journal of Modern Power Systems and Clean Energy》 SCIE EI 2013年第2期127-133,共7页
The modal analysis method is utilized to study the influence of doubly-fed induction generator(DFIG)on electromechanical oscillations.On this basis,the small signal stability region(SSSR)of power systems with DFIG in ... The modal analysis method is utilized to study the influence of doubly-fed induction generator(DFIG)on electromechanical oscillations.On this basis,the small signal stability region(SSSR)of power systems with DFIG in injection space is evaluated and the corresponding relationship between SSSR boundary and electromechanical oscillations is analyzed.The effects of the locations of DFIG on SSSR are considered.It is found that the boundary of SSSR consists of several smooth surfaces,which can be approximated with hyper-planes in engineering application.With the integration of DFIG,SSSR becomes smaller,thus indicating the deterioration of the small signal stability of the system.The 11-bus system with four generators is used to illustrate the proposed method. 展开更多
关键词 Doubly-fed induction generator(DFIG) Modal analysis Electromechanical oscillation Small signal stability region(SSSR)
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Impact of Electric Vehicle Aggregator with Communication Time Delay on Stability Regions and Stability Delay Margins in Load Frequency Control System 被引量:4
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作者 Ausnain Naveed Sahin Sonmez Saffet Ayasun 《Journal of Modern Power Systems and Clean Energy》 SCIE EI CSCD 2021年第3期595-601,共7页
This paper investigates the impact of electric vehicle(EV)aggregator with communication time delay on stability regions and stability delay margins of a single-area load frequency control(LFC)system.Primarily,a graphi... This paper investigates the impact of electric vehicle(EV)aggregator with communication time delay on stability regions and stability delay margins of a single-area load frequency control(LFC)system.Primarily,a graphical method characterizing stability boundary locus is implemented.For a given time delay,the method computes all the stabilizing proportional-integral(PI)controller gains,which constitutes a stability region in the parameter space of PI controller.Secondly,in order to complement the stability regions,a frequency-domain exact method is used to calculate stability delay margins for various values of PI controller gains.The qualitative impact of EV aggregator on both stability regions and stability delay margins is thoroughly analyzed and the results are authenticated by time-domain simulations and quasi-polynomial mapping-based root finder(QPmR)algorithm. 展开更多
关键词 Communication time delay electric vehicle(EV)aggregator frequency regulation proportional-integral(PI)controller design stability delay margin stability region
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Aircraft nonlinear stability analysis and multidimensional stability region estimation under icing conditions 被引量:2
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作者 Liang QU Yinghui LI +2 位作者 Haojun XU Dengcheng ZHANG Guoqiang YUAN 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2017年第3期976-982,共7页
Icing is one of the crucial factors that could pose great threat to flight safety,and thus research on stability and stability region of aircraft safety under icing conditions is significant for control and flight.Non... Icing is one of the crucial factors that could pose great threat to flight safety,and thus research on stability and stability region of aircraft safety under icing conditions is significant for control and flight.Nonlinear dynamical equations and models of aerodynamic coefficients of an aircraft are set up in this paper to study the stability and stability region of the aircraft under an icing condition.Firstly,the equilibrium points of the iced aircraft system are calculated and analyzed based on the theory of differential equation stability.Secondly,according to the correlation theory about equilibrium points and the stability region,this paper estimates the multidimensional stability region of the aircraft,based on which the stability regions before and after icing are compared.Finally,the results are confirmed by the time history analysis.The results can give a reference for stability analysis and envelope protection of the nonlinear system of an iced aircraft. 展开更多
关键词 Dynamic system Equilibrium points Icing aircraft Nonlinear stability stability region
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Stability of HIV-1 subtype B and C Tat is associated with variation in the carboxyl-terminal region 被引量:1
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作者 Xuechao Zhao Lingyu Qian +3 位作者 Deyu Zhou Di Qi Chang Liu Xiaohong Kong 《Virologica Sinica》 SCIE CAS CSCD 2016年第3期199-206,共8页
The multifunctional trans-activator Tat is an essential regulatory protein for HIV-1 replication and is characterized by high sequence diversity. Numerous experimental studies have examined Tat in HIV-1 subtype B, but... The multifunctional trans-activator Tat is an essential regulatory protein for HIV-1 replication and is characterized by high sequence diversity. Numerous experimental studies have examined Tat in HIV-1 subtype B, but research on subtype C Tat is lacking, despite the high prevalence of infections caused by subtype C worldwide. We hypothesized that amino acid differences contribute to functional differences among Tat proteins. In the present study, we found that subtype B NL4-3Tat and subtype C isolate HIV1084 i Tat exhibited differences in stability by overexpressing the fusion protein Tat-Flag. In addition, 1084 i Tat can activate LTR and NF-κB more efficiently than NL4-3 Tat. In analyses of the activities of the truncated forms of Tat, we found that the carboxylterminal region of Tat regulates its stability and transactivity. According to our results, we speculated that the differences in stability between B-Tat and C-Tat result in differences in transactivation ability. 展开更多
关键词 Tat subtype B/C stability transactivation carboxyl-terminal region NF-κB activation
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