Differentially Expressed Genes Related to Fertility Conversion in Thermo-sensitive Genic Male Sterile(TGMS) Lines of Brassica juncea

[Objective] This study was performed to screen functional genes related to the fertility conversion of thermo-sensitive genic male sterile （TGMS） lines of Brassica juncea L. [Method] A B. juncea TGMS line K121S was selected as the experimental material. The total RNAs were isolated from fertile and sterile pollens at different development stages, including mother cell stage, tetrad stage, tricellular pollen stage and maturity stage. DDRT-PCR was carried out to identify differentially expressed genes. [Result] A total of 44 differentially expressed cDNA fragments were identified with Dot blot. And seven candidate genes related to fertility conversion of K121S were screened out by BLASTN, including callose synthase gene, aldehyde dehydrogenase gene and RNA polymerase I transcription factor RRN3 gene which were differentially expressed at the transcriptional level, H＇-ATPase gene, fructose diphosphate aldolase -class I gene, teucine-rich repeat receptor-Jike serine/threonine- protein kinase gene and alkaline/neutral invertase gene, which were differentially expressed at the post-transcriptional level. [Conclusion] The results of this study will help to explain the molecular mechanism of thermo-sensitive genic male sterility of B. juncea.

Lukasiewicz and Lorenzen as Interpreters of Aristotle＇s Syllogistic

Jan Lukasiewicz acknowledged that Aristotle＇s syllogistic does not admit singular terms and presents syllogism as an implication. But he failed to recognize syllogistic necessity, reducing this necessity to ＂formal implication＂ as introduced by Russell, when Aristotle shows it as binding relations between three terms. On the contrary, Paul Lorenzen directly recognized syllogistic necessity as the typical example of his own logical implication. His reconstruction of syllogistic differs from the original by his interpretation of particular propositions as the determination of classes which are specified by predicates. The result is the representation of valid moods as the board of all multiplications of relations which are permitted. These relations are not only the Aristotelian ,4, E,I, O, but also the new converse asymmetrical relations of A and O： （a） and （o）.

Application of Question and Answering on Virtual Human Dialogue:a Review and Prediction

Nowadays,virtual human(VH) is becoming a hot research topic in virtualization.VH dialogue can be categorized as an application of natural language processing(NLP) technology,since it is relational to question and answering(QA) technologies.In order to integrate these technologies,this paper reviews some important work on VH dialogue,and predicts some research points on the view of QA technologies.

Simultaneous Determination of Seven Components in Gamboge and Its Processed Products Using a Single Reference Standard

Objective To establish a quality control method for simultaneous determination of multiple components in gamboge. Methods A single reference standard for the determination of multiple components （SSDMC） with HPLC was proposed. Seven major components of gamboge including gambogenic acid （S）, β-morellic acid （C1）, 2R-30-hydroxygambogic acid （C2）, isogambogenic acid （C3）, gambogellic acid （C4）, 2R-gambogic acid （C5）, and 2S-gambogic acid （C6） were simultaneously analyzed using gambogenic acid as reference standard. The credibility and feasibility of SSDMC method were validated with respect to linearity, limits of detection and quantification, precision, stability, repeatability, accuracy, ruggedness, and robustness. The relative conversion factors （RCFs） of S and C1-6 were calculated. Twelve batches of gamboge including crude and processed products were successfully analyzed by applying the SSDMC and traditional external standard （ES） methods. Results The SSDMC method was credible and feasible. The RCFs of S and C1-6 were 1.000, 0.913, 0.864, 1.064, 0.777, 0.921, and 0.919, respectively. No significant difference was observed in the contents of the seven components between SSDMC and ES methods. The heat-processing technique caused a reduction in the seven components. Conclusion SSDMC is a simple, reliable, and effective method for the analysis of the complex multiple components in gamboge, and it is also a practical and economical approach.