The expression levels and changes of endogenous acid fibroblast growth factor （aFGF） in microwave burn wound tissues were detected in order to investigate how to get better therapeutic effects by using the exogenous...The expression levels and changes of endogenous acid fibroblast growth factor （aFGF） in microwave burn wound tissues were detected in order to investigate how to get better therapeutic effects by using the exogenous aFGF for repairing trauma. A burnt-wound animal model was established by NS-FⅡ multifunction spectrum therapeutics equipment, and reverse transcriptase-polymerase chain reaction （RT-PCR） and immunohistochemistry assay were applied to detect the expression levels of endogenous aFGF mRNA in microwave burn wound tissues. The expression level of endogenous aFGF mRNA was significantly increased in the burn wound tissues 12 h after burn, reached the peak at 48 h, and gradually deceased 96 h after burn. The expression of endogenous aFGF mRNA after tissue damage was reversible, and its intensity was in accordance with the repair process of tissue damage, suggesting endogenous aFGF may take part in the cell metabolism and proliferation, and then promote the repair of the burn wound.展开更多
基金This project was supported by a grant from Foundation of National 863 Program for Research and Development of Class I Novel Drug rh-aFGF (No 2002AA2Z3349)
文摘The expression levels and changes of endogenous acid fibroblast growth factor （aFGF） in microwave burn wound tissues were detected in order to investigate how to get better therapeutic effects by using the exogenous aFGF for repairing trauma. A burnt-wound animal model was established by NS-FⅡ multifunction spectrum therapeutics equipment, and reverse transcriptase-polymerase chain reaction （RT-PCR） and immunohistochemistry assay were applied to detect the expression levels of endogenous aFGF mRNA in microwave burn wound tissues. The expression level of endogenous aFGF mRNA was significantly increased in the burn wound tissues 12 h after burn, reached the peak at 48 h, and gradually deceased 96 h after burn. The expression of endogenous aFGF mRNA after tissue damage was reversible, and its intensity was in accordance with the repair process of tissue damage, suggesting endogenous aFGF may take part in the cell metabolism and proliferation, and then promote the repair of the burn wound.
