Environmental endocrine disrupting chemicals （EDCs）, commonly found in the environment, come from industry and agriculture, including pesticides, fungicides, insecticides, herbicides, and other chemicals. Nowadays, ...Environmental endocrine disrupting chemicals （EDCs）, commonly found in the environment, come from industry and agriculture, including pesticides, fungicides, insecticides, herbicides, and other chemicals. Nowadays, more and more EDCs were released into the environment. EDCs go into water body via atmosphere sedi-mentation, surface runoff, soil eluviation, etc., so water body becomes the main place for existing. In order to attract scientific and public attention worldwide and to prevent EDCs pol ution, in this study we reviewed the classification of EDCs and their concentrations in natural water bodies, drinking water sources and water plants, and the reproductive toxicity of EDCs to fish were reviewed. EDCs could disturb the endocrine system and make reproductive organs and reproduction abnor-mal, resulting in fertility descending, reproduction function damage, community quan-tity decrease and even species extinction. In addition, EDCs could disrupt the homeostasis maintained by hormones, which would result in defects of neural de-velopment and abnormalities of the endocrine and reproductive systems. The exact molecular mechanisms have not been completely reported, but researches have suggested that multiple mechanisms were involved in the action of EDCs. Although there have been researches on the biohazard of EDCs, there stil exist problems of weakness in fundamental researches, difficulties in recognizing and identifying EDCs and high cost, which restraint the knowledge on them.展开更多
To study the effect of endocrine disruptor chemicals in cow milk on female reproductive system. Methods A two-generation reproduction was conducted according to U. S. FDA standard. Milk was fed in special bottle to Wi...To study the effect of endocrine disruptor chemicals in cow milk on female reproductive system. Methods A two-generation reproduction was conducted according to U. S. FDA standard. Milk was fed in special bottle to Wistar rats of both sexes through two successive generations （F0 and F1） in the milk group while artificial milk was fed to rats in the control group. Twenty-four rats of each sex were mated in each group. Measurements were made according to this guideline. Results Reproductive parameters in the milk group such as fertility index, gestation index, weights of uterus and ovary, days of vaginal opening, estrous cycles, histological morphological changes were comparable to those in the control group. However, the means of body weight had some differences. The body weight gains increased significantly in the milk-treated group in F1 and F2 generation compared with those in the control group. The concentration of insulin-like growth factor-1 （IGF-1） in blood in the milk group was comparable to that in the control group, but the standard deviation changed greatly in the milk-treated rats. Conclusion Endocrine disruptor chemicals in milk have no severe effects on the female reproductive system.展开更多
In 1956, my former Head and Professor of the Department of Obstetrics and Gynecology KT LIM, asked me to take the initiative to establish a reproductive endocrinology unit. As China is a developing country, we wer... In 1956, my former Head and Professor of the Department of Obstetrics and Gynecology KT LIM, asked me to take the initiative to establish a reproductive endocrinology unit. As China is a developing country, we were then short of diagnostic facilities and therapeutic agents. We can learn basic knowledge and clinical experiences from the literature, but hormonal preparations were expensive and generally most of our patients were poor. We needed to find simpler, inexpensive and yet effective ways to treat our patients. In a word, we had to develop our own way.……展开更多
Juvenile hormone(JH)acts in the regulation of caste differentiation between queens and workers(i.e.,with or without reproductive capacity)during vitellin synthesis and oogenesis in social insects.However,the regulator...Juvenile hormone(JH)acts in the regulation of caste differentiation between queens and workers(i.e.,with or without reproductive capacity)during vitellin synthesis and oogenesis in social insects.However,the regulatory mechanisms have not yet been elucidated.Here,we identified a highly expressed microRNA(miRNA),miR-1175-3p,in the red imported fire ant,Solenopsis invicta.We found that miR-1175-3p is prominently present in the fat bodies and ovaries of workers.Furthermore,miR-1175-3p interacts with its target gene,broad-complex core(Br-C),in the fat bodies.By utilizing miR-1175-3p agomir,we successfully suppressed the expression of the Br-C protein in queens,resulting in reduced vitellogenin expression,fewer eggs,and poorly developed ovaries.Conversely,decreasing miR-1175-3p levels led to the increased expression of Br-C and vitellogenin in workers,triggering the“re-development”of the ovaries.Moreover,when queens were fed with JH,the expression of miR-1175-3p decreased,whereas the expression of vitellogenin-2 and vitellogenin-3 increased.Notably,the suppression of fertility in queens caused by treatment with agomir miR-1175-3p was completely rescued by the increased vitellogenin expression induced by being fed with JH.These results suggest the critical role of miR-1175-3p in JH-regulated reproduction,shedding light on the molecular mechanism underlying miRNA-mediated fecundity in social insects and providing a novel strategy for managing S.invicta.展开更多
The reproductive endocrine activities of neuroendocrine organs and androgenic glands (AG) in male Scylla serrata were investigated with co-incubation technology. In vitro studies show that: (1) the AG in Stage Ⅲ can ...The reproductive endocrine activities of neuroendocrine organs and androgenic glands (AG) in male Scylla serrata were investigated with co-incubation technology. In vitro studies show that: (1) the AG in Stage Ⅲ can significantly accelerate the development of seminiferous tubules and spermic matu- ration; (2) the brain, thoracic ganglia and optic ganglia have no direct influence over the testicular devel- opment; (3) the brain and thoracic ganglia can significantly promote the growth of the AG cells and highly significantly boost the ratio of Type B cells, while the optic ganglia have no such effects. It is the first time for in vitro investigations to confirm that the brain and thoracic ganglia can regulate testicular development through AG in male crustaceans.展开更多
Objective:To evaluate the potential of Gracilaria changii extract in ameliorating the potential adverse effects of bisphenol A.Methods:The antioxidant capacity of Gracilaria changii extracted using different solvents(...Objective:To evaluate the potential of Gracilaria changii extract in ameliorating the potential adverse effects of bisphenol A.Methods:The antioxidant capacity of Gracilaria changii extracted using different solvents(methanol,ethanol,and aqueous)was studied.The mice were administered by oral gavage with bisphenol A(60 mg/kg body weight)for 6 weeks with or without Gracilaria changii aqueous extract.Thereafter,the mice were either euthanized for histology and immunohistochemistry studies or mated to evaluate the pregnancy rate.Results:Gracilaria changii aqueous extract showed the highest antioxidant properties compared with extract using methanol and ethanol.The aqueous extract of Gracilaria changii improved the uterus index and uterine lipid peroxidation after bisphenol A exposure,although the uterine expressions of estrogen receptors and complement C3 were not improved.Histological evaluation of the uterus during the estrus stage has revealed that the extract could mitigate bisphenol A-induced adverse effects in the uterus as there was a lower percentage of mice showing abnormalities like decreased eosin staining in the myometrium,and decrease in the number of eosinophil and endometrial glands in the endometrium.Besides,Gracilaria changii aqueous extract improved the pregnancy rate of mice administered with bisphenol A.Conclusions:Gracilaria changii extract protects against bisphenol A-induced female reproductive abnormalities in mice which may be mediated via modulation of eosinophil migration,endometrial gland formation,and protein expressions associated with prostaglandins in the myometrium.展开更多
Di(2-ethylhexyl)phthalate(DEHP),an environ-mental endocrine disruptor,has hormone-like activity and endocrine-disrupting effects.However,the types of reproductive hormones associated with DEHP vary across the studies....Di(2-ethylhexyl)phthalate(DEHP),an environ-mental endocrine disruptor,has hormone-like activity and endocrine-disrupting effects.However,the types of reproductive hormones associated with DEHP vary across the studies.Thus,we conducted a systematic review and meta-analysis to pool existing epidemiological evidence.We searched three databases up to January 31,2024,for eligible original studies to ultimately include 37 studies from eight countries with a total of 28911 participants.DEHP exposure was evaluated with urinary metabolites.Since the main types,production sites,blood concentrations,and functions of reproductive hormones differ between men and women,we reported the combined effect values by gender.Subgroup analyses were conducted by age,subfertility status,and the national sociodemographic index(SDI)level.Furthermore,the effect of maternal exposure during pregnancy on children’s reproductive hormone levels was analyzed separately.Overall,in general,in men,DEHP was positively correlated with sex hormone binding-globulin(SHBG)and adversely correlated with total testosterone(TT),free androgen index(FAI),and follicle-stimulating hormone(FSH).Results indicated that among men of reproductive age,DEHP exposure was associated with more significant hormonal suppression in infertile men compared with fertile men.Notably,age subgroup analysis among women revealed that postmenopausal women were more vulnerable to DEHP,which was related to lower TT and estradiol(E2).However,this study did not observe a significant association between prenatal DEHP metabolites and reproductive hormone levels in children.Our research identifies the most susceptible hormones(androgen suppression)after DEHP exposure and suggests that infertile men and postmenopausal women are in great need of more attention as sensitive populations.展开更多
基金Supported by the China Agriculture Research System(CARS49)~~
文摘Environmental endocrine disrupting chemicals （EDCs）, commonly found in the environment, come from industry and agriculture, including pesticides, fungicides, insecticides, herbicides, and other chemicals. Nowadays, more and more EDCs were released into the environment. EDCs go into water body via atmosphere sedi-mentation, surface runoff, soil eluviation, etc., so water body becomes the main place for existing. In order to attract scientific and public attention worldwide and to prevent EDCs pol ution, in this study we reviewed the classification of EDCs and their concentrations in natural water bodies, drinking water sources and water plants, and the reproductive toxicity of EDCs to fish were reviewed. EDCs could disturb the endocrine system and make reproductive organs and reproduction abnor-mal, resulting in fertility descending, reproduction function damage, community quan-tity decrease and even species extinction. In addition, EDCs could disrupt the homeostasis maintained by hormones, which would result in defects of neural de-velopment and abnormalities of the endocrine and reproductive systems. The exact molecular mechanisms have not been completely reported, but researches have suggested that multiple mechanisms were involved in the action of EDCs. Although there have been researches on the biohazard of EDCs, there stil exist problems of weakness in fundamental researches, difficulties in recognizing and identifying EDCs and high cost, which restraint the knowledge on them.
文摘To study the effect of endocrine disruptor chemicals in cow milk on female reproductive system. Methods A two-generation reproduction was conducted according to U. S. FDA standard. Milk was fed in special bottle to Wistar rats of both sexes through two successive generations （F0 and F1） in the milk group while artificial milk was fed to rats in the control group. Twenty-four rats of each sex were mated in each group. Measurements were made according to this guideline. Results Reproductive parameters in the milk group such as fertility index, gestation index, weights of uterus and ovary, days of vaginal opening, estrous cycles, histological morphological changes were comparable to those in the control group. However, the means of body weight had some differences. The body weight gains increased significantly in the milk-treated group in F1 and F2 generation compared with those in the control group. The concentration of insulin-like growth factor-1 （IGF-1） in blood in the milk group was comparable to that in the control group, but the standard deviation changed greatly in the milk-treated rats. Conclusion Endocrine disruptor chemicals in milk have no severe effects on the female reproductive system.
文摘 In 1956, my former Head and Professor of the Department of Obstetrics and Gynecology KT LIM, asked me to take the initiative to establish a reproductive endocrinology unit. As China is a developing country, we were then short of diagnostic facilities and therapeutic agents. We can learn basic knowledge and clinical experiences from the literature, but hormonal preparations were expensive and generally most of our patients were poor. We needed to find simpler, inexpensive and yet effective ways to treat our patients. In a word, we had to develop our own way.……
基金National Key R&D Program of China(2021YFD1000500)the Natural Science Foundation of Guangdong Province(2023A1515012108)the Foundation of Guangdong Academy of Agricultural Sciences(R2023PY-JX010).
文摘Juvenile hormone(JH)acts in the regulation of caste differentiation between queens and workers(i.e.,with or without reproductive capacity)during vitellin synthesis and oogenesis in social insects.However,the regulatory mechanisms have not yet been elucidated.Here,we identified a highly expressed microRNA(miRNA),miR-1175-3p,in the red imported fire ant,Solenopsis invicta.We found that miR-1175-3p is prominently present in the fat bodies and ovaries of workers.Furthermore,miR-1175-3p interacts with its target gene,broad-complex core(Br-C),in the fat bodies.By utilizing miR-1175-3p agomir,we successfully suppressed the expression of the Br-C protein in queens,resulting in reduced vitellogenin expression,fewer eggs,and poorly developed ovaries.Conversely,decreasing miR-1175-3p levels led to the increased expression of Br-C and vitellogenin in workers,triggering the“re-development”of the ovaries.Moreover,when queens were fed with JH,the expression of miR-1175-3p decreased,whereas the expression of vitellogenin-2 and vitellogenin-3 increased.Notably,the suppression of fertility in queens caused by treatment with agomir miR-1175-3p was completely rescued by the increased vitellogenin expression induced by being fed with JH.These results suggest the critical role of miR-1175-3p in JH-regulated reproduction,shedding light on the molecular mechanism underlying miRNA-mediated fecundity in social insects and providing a novel strategy for managing S.invicta.
基金This work was supported by the National Natural Science Foundation of China (No.30300269) and the Fujian Province Key Foundation Re-search Program (1998-2002).
文摘The reproductive endocrine activities of neuroendocrine organs and androgenic glands (AG) in male Scylla serrata were investigated with co-incubation technology. In vitro studies show that: (1) the AG in Stage Ⅲ can significantly accelerate the development of seminiferous tubules and spermic matu- ration; (2) the brain, thoracic ganglia and optic ganglia have no direct influence over the testicular devel- opment; (3) the brain and thoracic ganglia can significantly promote the growth of the AG cells and highly significantly boost the ratio of Type B cells, while the optic ganglia have no such effects. It is the first time for in vitro investigations to confirm that the brain and thoracic ganglia can regulate testicular development through AG in male crustaceans.
基金supported by the Universiti Tunku Abdul Rahman(UTAR)Research Fund(IPSR/RMC/UTARRF 2017-C1/K04)
文摘Objective:To evaluate the potential of Gracilaria changii extract in ameliorating the potential adverse effects of bisphenol A.Methods:The antioxidant capacity of Gracilaria changii extracted using different solvents(methanol,ethanol,and aqueous)was studied.The mice were administered by oral gavage with bisphenol A(60 mg/kg body weight)for 6 weeks with or without Gracilaria changii aqueous extract.Thereafter,the mice were either euthanized for histology and immunohistochemistry studies or mated to evaluate the pregnancy rate.Results:Gracilaria changii aqueous extract showed the highest antioxidant properties compared with extract using methanol and ethanol.The aqueous extract of Gracilaria changii improved the uterus index and uterine lipid peroxidation after bisphenol A exposure,although the uterine expressions of estrogen receptors and complement C3 were not improved.Histological evaluation of the uterus during the estrus stage has revealed that the extract could mitigate bisphenol A-induced adverse effects in the uterus as there was a lower percentage of mice showing abnormalities like decreased eosin staining in the myometrium,and decrease in the number of eosinophil and endometrial glands in the endometrium.Besides,Gracilaria changii aqueous extract improved the pregnancy rate of mice administered with bisphenol A.Conclusions:Gracilaria changii extract protects against bisphenol A-induced female reproductive abnormalities in mice which may be mediated via modulation of eosinophil migration,endometrial gland formation,and protein expressions associated with prostaglandins in the myometrium.
基金supported by the National Natural Science Foundation of China(Grant No.42375184 and 42105165)Research Funds of Center for Big Data and Population Health of IHM(Grant No.JKS2022011)Natural Science Research Key Project of University of Anhui Province(Grant No.2023AH050652).
文摘Di(2-ethylhexyl)phthalate(DEHP),an environ-mental endocrine disruptor,has hormone-like activity and endocrine-disrupting effects.However,the types of reproductive hormones associated with DEHP vary across the studies.Thus,we conducted a systematic review and meta-analysis to pool existing epidemiological evidence.We searched three databases up to January 31,2024,for eligible original studies to ultimately include 37 studies from eight countries with a total of 28911 participants.DEHP exposure was evaluated with urinary metabolites.Since the main types,production sites,blood concentrations,and functions of reproductive hormones differ between men and women,we reported the combined effect values by gender.Subgroup analyses were conducted by age,subfertility status,and the national sociodemographic index(SDI)level.Furthermore,the effect of maternal exposure during pregnancy on children’s reproductive hormone levels was analyzed separately.Overall,in general,in men,DEHP was positively correlated with sex hormone binding-globulin(SHBG)and adversely correlated with total testosterone(TT),free androgen index(FAI),and follicle-stimulating hormone(FSH).Results indicated that among men of reproductive age,DEHP exposure was associated with more significant hormonal suppression in infertile men compared with fertile men.Notably,age subgroup analysis among women revealed that postmenopausal women were more vulnerable to DEHP,which was related to lower TT and estradiol(E2).However,this study did not observe a significant association between prenatal DEHP metabolites and reproductive hormone levels in children.Our research identifies the most susceptible hormones(androgen suppression)after DEHP exposure and suggests that infertile men and postmenopausal women are in great need of more attention as sensitive populations.
