Helicobacter pylori(H.pylori)was reported to be associated with gastric carcinogenesis.Resistin-like molecule beta(RELMβ),a recently described goblet cell-specific protein,was demonstrated to aberrantly express in ga...Helicobacter pylori(H.pylori)was reported to be associated with gastric carcinogenesis.Resistin-like molecule beta(RELMβ),a recently described goblet cell-specific protein,was demonstrated to aberrantly express in gastric cancer and correlated with its clinicopathological features.This study aimed to examine the association between H.pylori and RELMp expression in gastric carcinoma and precursor lesions.H.pylori infection and RELMβexpression were immunohistochemically evaluated in gastric biopsies from 230 patients.The biopsies consisted of normal gastric mucosa(w=20),mucosa with chronic gastritis(n=41),intestinal metaplasia(n=42),dysplasia(w=31),intestinal-type adenocarcinoma(n=56),and diffuse-type adenocarcinoma(n=40).RELMβ expression was measured in gastric biopsies after H.pylori eradication therapy in a subgroup of 32 patients.Cultured gastric cancer cell line SGC-7901 was infected with H.pylori strains,and RELMp expression was detected by reverse transcription PCR,real-time PCR and Western blotting.Higher RELMP immunoreactivity was observed in H.pyloripositive intestinal metaplasia(P=0.003),dysplasia(P=0.032),intestinal-type(P=0.037)and diffuse-type adenocarcinomas(P=0.001)than in H.pylori-negative specimens.Expression rates of RELMβ in dysplasia(CO.005),intestinal-type adenocarcinoma(P<0.001),and diffuse-type adenocarcinoma(P=0.001)were significantly correlated with the grade of H.pylori density.In addition,H.pylori eradication reduced the RELMβintensity in intestinal metaplasia(P=0.001).Infection of gastric cancer SGC-7901 cells with cag pathogenicity island(PAI)-positive H.pylori TN2,but not with its PAI totally deleted mutant(TN2-APAI)for 4-8 h,resulted in enhanced protein and transcript levels of RELMβ(P<0.05).In summary,our study suggested that H.pylori infection facilitated the expression of RELMβ in gastric garcinoma and precursor lesions.展开更多
Previous studies have confirmed that telencephalin （TLN） is a neural glycoprotein that protects axonal disruption induced by the beta-amyloid protein （Aβ42/35） in the neural crest-derived tumor cell line Paju. Th...Previous studies have confirmed that telencephalin （TLN） is a neural glycoprotein that protects axonal disruption induced by the beta-amyloid protein （Aβ42/35） in the neural crest-derived tumor cell line Paju. The present study investigated the effects of TLN on neuronal degeneration induced by Aβ42 in the differentiated Paju cell line. Results demonstrated that after cultivating cells in Aβ42 medium, the survival rate of Paju-TLN cells was significantly higher than that of Paju-neo cells, and that apoptotic rate was noticeably reduced. These results indicate that TLN reduces Paju cell apoptosis induced by Aβ42.展开更多
Telencephalin is a neural glycoprotein that reduces apoptosis induced by amyloid beta protein in the human neural tumor cell line PAJU. In this study, we examined the role of the ezrin/radixin/moesin protein family/ph...Telencephalin is a neural glycoprotein that reduces apoptosis induced by amyloid beta protein in the human neural tumor cell line PAJU. In this study, we examined the role of the ezrin/radixin/moesin protein family/phosphatidylinositol-3-kinase/protein kinase B pathway in this process. Western blot analysis demonstrated that telencephalin, phosphorylated ezrin/radixin/moesin and phosphatidylinositol-3-kinase/protein kinase B were not expressed in PAJU cells transfected with empty plasmid, while they were expressed in PAJU cells transfected with a telencephalin expression plasmid. After treatment with 1.0 nM amyloid beta protein 42, expression of telencephalin and phosphorylated phosphatidylinositol-3-kinase/protein kinase B in the transfected cells gradually diminished, while levels of phosphorylated ezrin/radixin/moesin increased. In addition, the high levels of telencephalin, phosphorylated ezrin/radixin/moesin and phosphatidylinositol-3-kinase/protein kinase B expression in PAJU cells transfected with a telencephalin expression plasmid could be suppressed by the phosphatidylinositol-3-kinase inhibitor LY294002. These findings indicate that telencephalin activates the ezrin/radixin/moesin family/phosphatidylinositol-3-kinase/protein kinase B pathway and protects PAJU cells from amyloid beta protein-induced apoptosis.展开更多
目的:研究抵抗素样分子β(resistin like molecule beta,RELMβ)在慢性阻塞性肺疾病(COPD)患者肺组织中的分布及表达。方法:采集暨南大学附属第一医院因肺癌入院行肺叶切除术的19例肺组织标本,分为COPD组(10例)和对照组(9例)。采用HE染...目的:研究抵抗素样分子β(resistin like molecule beta,RELMβ)在慢性阻塞性肺疾病(COPD)患者肺组织中的分布及表达。方法:采集暨南大学附属第一医院因肺癌入院行肺叶切除术的19例肺组织标本,分为COPD组(10例)和对照组(9例)。采用HE染色、免疫组织化学染色及Western blot方法,检测RELMβ蛋白的表达情况。结果:COPD组患者肺组织中RELMβ的表达(2.657±0.387)明显高于肺功能正常组(0.386±0.072)(P<0.05),且与肺功能主要指标(FEV1%预计值、FEV1/FVC%)呈负相关;与吸烟指数呈正相关(P值均<0.05)。结论:RELMβ可能参与慢性阻塞性肺疾病的发病过程。展开更多
基金the National Natural Science Foundation of China(Nos.81071997,81072073).
文摘Helicobacter pylori(H.pylori)was reported to be associated with gastric carcinogenesis.Resistin-like molecule beta(RELMβ),a recently described goblet cell-specific protein,was demonstrated to aberrantly express in gastric cancer and correlated with its clinicopathological features.This study aimed to examine the association between H.pylori and RELMp expression in gastric carcinoma and precursor lesions.H.pylori infection and RELMβexpression were immunohistochemically evaluated in gastric biopsies from 230 patients.The biopsies consisted of normal gastric mucosa(w=20),mucosa with chronic gastritis(n=41),intestinal metaplasia(n=42),dysplasia(w=31),intestinal-type adenocarcinoma(n=56),and diffuse-type adenocarcinoma(n=40).RELMβ expression was measured in gastric biopsies after H.pylori eradication therapy in a subgroup of 32 patients.Cultured gastric cancer cell line SGC-7901 was infected with H.pylori strains,and RELMp expression was detected by reverse transcription PCR,real-time PCR and Western blotting.Higher RELMP immunoreactivity was observed in H.pyloripositive intestinal metaplasia(P=0.003),dysplasia(P=0.032),intestinal-type(P=0.037)and diffuse-type adenocarcinomas(P=0.001)than in H.pylori-negative specimens.Expression rates of RELMβ in dysplasia(CO.005),intestinal-type adenocarcinoma(P<0.001),and diffuse-type adenocarcinoma(P=0.001)were significantly correlated with the grade of H.pylori density.In addition,H.pylori eradication reduced the RELMβintensity in intestinal metaplasia(P=0.001).Infection of gastric cancer SGC-7901 cells with cag pathogenicity island(PAI)-positive H.pylori TN2,but not with its PAI totally deleted mutant(TN2-APAI)for 4-8 h,resulted in enhanced protein and transcript levels of RELMβ(P<0.05).In summary,our study suggested that H.pylori infection facilitated the expression of RELMβ in gastric garcinoma and precursor lesions.
文摘Previous studies have confirmed that telencephalin （TLN） is a neural glycoprotein that protects axonal disruption induced by the beta-amyloid protein （Aβ42/35） in the neural crest-derived tumor cell line Paju. The present study investigated the effects of TLN on neuronal degeneration induced by Aβ42 in the differentiated Paju cell line. Results demonstrated that after cultivating cells in Aβ42 medium, the survival rate of Paju-TLN cells was significantly higher than that of Paju-neo cells, and that apoptotic rate was noticeably reduced. These results indicate that TLN reduces Paju cell apoptosis induced by Aβ42.
基金supported by a grant under Key Projects of Guangxi Traditional Chinese Medical University, No.ZD2007041
文摘Telencephalin is a neural glycoprotein that reduces apoptosis induced by amyloid beta protein in the human neural tumor cell line PAJU. In this study, we examined the role of the ezrin/radixin/moesin protein family/phosphatidylinositol-3-kinase/protein kinase B pathway in this process. Western blot analysis demonstrated that telencephalin, phosphorylated ezrin/radixin/moesin and phosphatidylinositol-3-kinase/protein kinase B were not expressed in PAJU cells transfected with empty plasmid, while they were expressed in PAJU cells transfected with a telencephalin expression plasmid. After treatment with 1.0 nM amyloid beta protein 42, expression of telencephalin and phosphorylated phosphatidylinositol-3-kinase/protein kinase B in the transfected cells gradually diminished, while levels of phosphorylated ezrin/radixin/moesin increased. In addition, the high levels of telencephalin, phosphorylated ezrin/radixin/moesin and phosphatidylinositol-3-kinase/protein kinase B expression in PAJU cells transfected with a telencephalin expression plasmid could be suppressed by the phosphatidylinositol-3-kinase inhibitor LY294002. These findings indicate that telencephalin activates the ezrin/radixin/moesin family/phosphatidylinositol-3-kinase/protein kinase B pathway and protects PAJU cells from amyloid beta protein-induced apoptosis.
文摘目的:研究抵抗素样分子β(resistin like molecule beta,RELMβ)在慢性阻塞性肺疾病(COPD)患者肺组织中的分布及表达。方法:采集暨南大学附属第一医院因肺癌入院行肺叶切除术的19例肺组织标本,分为COPD组(10例)和对照组(9例)。采用HE染色、免疫组织化学染色及Western blot方法,检测RELMβ蛋白的表达情况。结果:COPD组患者肺组织中RELMβ的表达(2.657±0.387)明显高于肺功能正常组(0.386±0.072)(P<0.05),且与肺功能主要指标(FEV1%预计值、FEV1/FVC%)呈负相关;与吸烟指数呈正相关(P值均<0.05)。结论:RELMβ可能参与慢性阻塞性肺疾病的发病过程。
