In pH 7.2 Na2HPO4-NaH2PO4 buffer solution and in the （GABF） was combined with human factor B （BF） specifically, presence of PEG-6000, goat-anti-human factor B and aggregated to form immune complex particles that e...In pH 7.2 Na2HPO4-NaH2PO4 buffer solution and in the （GABF） was combined with human factor B （BF） specifically, presence of PEG-6000, goat-anti-human factor B and aggregated to form immune complex particles that exhibited a resonance scattering （RS） peak at 400 nm. The laser scattering indicated that the average diameter of immune complex particles was 1320 nm. BF in the concentration range of 0.04 to 9.60 μg/mL was proportional to the resonance scattering intensity at 400 nm. Its regression equation was A/=33.61C ＋ 1.4, with a correlation coefficient of 0.9969, and a detection limit of 0.01 μg/mL BF. This label-free resonance scattering spectral （RSS） method has been applied to the determination of BF in serum samples, and the results were in agreement with that of the immunoturbity.展开更多
In pH 7.2 Na2HPO4-NaH2PO4 buffer solution, polyphenoloxidase （PPO） could catalyze the oxidization of do- pamine （DA） to form polymer particles in dark-red color, which exhibited a strong resonance scattering （RS...In pH 7.2 Na2HPO4-NaH2PO4 buffer solution, polyphenoloxidase （PPO） could catalyze the oxidization of do- pamine （DA） to form polymer particles in dark-red color, which exhibited a strong resonance scattering （RS） peak at 780 nm. In the chosen conditions, as the PPO activity increased, the RS intensity at 780 nm increased linearly. The increased RS intensity （△I780nm） was linear to the PPO activity in the range of 0.10--6.0 U·mL^- 1, with a regression equation of △I780 nm = 96.6C＋ 15.1, a relative coefficient of 0.9987 and a detection limit of 0.06 U·mL^-1 PPO. The proposed method was applied to detecting PPO activity in potato sample with satisfactory results.展开更多
The 5 nm AuPd nanoalloy in mole ratio of Au：Pd=32 ： 1 was prepared, using sodium citrate as the stabilizing agent and NaBH4 as the reductant. The AuPd nanoalloy was modified by the aptamer to prepare an aptamer- Au...The 5 nm AuPd nanoalloy in mole ratio of Au：Pd=32 ： 1 was prepared, using sodium citrate as the stabilizing agent and NaBH4 as the reductant. The AuPd nanoalloy was modified by the aptamer to prepare an aptamer- AuPd （AptAuPd） probe for resonance scattering （RS） detection of 5.（Y-1250 nmol/L Hg2＋. The AptAuPd-Hg2＋ aptamer reaction solution was filtrated by membrane, and the AptAuPd in the filtrate exhibited strong catalytic ef- fect on the slow NiP particle reaction between NiCI2 and NaHzPO2, and the NiP particles showed a RS peak at 508 nm. The RS intensity decreased when Hg2＋ concentration increased. The decreased RS intensity was linear to Hg2＋ concentration in the range of 0.5--1250 nmol/L. The RS assays were used to determine Hg2＋ in real samples, with good results.展开更多
基金Acknowledgement This work was supported by the National Natural Science Foundation of China （Nos. 21165005, 21075023）, the fund of State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Science and Technology of China （CMEMR2011-10）.
文摘In pH 7.2 Na2HPO4-NaH2PO4 buffer solution and in the （GABF） was combined with human factor B （BF） specifically, presence of PEG-6000, goat-anti-human factor B and aggregated to form immune complex particles that exhibited a resonance scattering （RS） peak at 400 nm. The laser scattering indicated that the average diameter of immune complex particles was 1320 nm. BF in the concentration range of 0.04 to 9.60 μg/mL was proportional to the resonance scattering intensity at 400 nm. Its regression equation was A/=33.61C ＋ 1.4, with a correlation coefficient of 0.9969, and a detection limit of 0.01 μg/mL BF. This label-free resonance scattering spectral （RSS） method has been applied to the determination of BF in serum samples, and the results were in agreement with that of the immunoturbity.
基金Project supported by the National Natural Science Foundation of China （Nos. 20865002, 20965002, 21075023）, the Natural Science Foundation ot Guangxi （Nos. 0991021 z, 0832260） and the Research Funds of Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection （Guangxi Normal University）, Ministry of Education.
文摘In pH 7.2 Na2HPO4-NaH2PO4 buffer solution, polyphenoloxidase （PPO） could catalyze the oxidization of do- pamine （DA） to form polymer particles in dark-red color, which exhibited a strong resonance scattering （RS） peak at 780 nm. In the chosen conditions, as the PPO activity increased, the RS intensity at 780 nm increased linearly. The increased RS intensity （△I780nm） was linear to the PPO activity in the range of 0.10--6.0 U·mL^- 1, with a regression equation of △I780 nm = 96.6C＋ 15.1, a relative coefficient of 0.9987 and a detection limit of 0.06 U·mL^-1 PPO. The proposed method was applied to detecting PPO activity in potato sample with satisfactory results.
基金Project supported by the National Natural Science Founclation of china （Nos. 21075023, 20965002, 20865002）, Natural Science Foundation of Guangxi （No. 0991021z）, the Research Funds of Guangxi Water Conservancy, the Research Funds of Key Laboratory of Ecology of Rare and En- dangered Species and Environmental Protection, Ministry of Education （No. 10012019） and the Research Funds of Guangxi Education Office.
文摘The 5 nm AuPd nanoalloy in mole ratio of Au：Pd=32 ： 1 was prepared, using sodium citrate as the stabilizing agent and NaBH4 as the reductant. The AuPd nanoalloy was modified by the aptamer to prepare an aptamer- AuPd （AptAuPd） probe for resonance scattering （RS） detection of 5.（Y-1250 nmol/L Hg2＋. The AptAuPd-Hg2＋ aptamer reaction solution was filtrated by membrane, and the AptAuPd in the filtrate exhibited strong catalytic ef- fect on the slow NiP particle reaction between NiCI2 and NaHzPO2, and the NiP particles showed a RS peak at 508 nm. The RS intensity decreased when Hg2＋ concentration increased. The decreased RS intensity was linear to Hg2＋ concentration in the range of 0.5--1250 nmol/L. The RS assays were used to determine Hg2＋ in real samples, with good results.
