Extract of the eschar tissue taken from the rat which had been given full-thickness burn of20% TBSA was found to be inhibitory to the respiratory function of the mitochondria and theenergization of the inner membrane....Extract of the eschar tissue taken from the rat which had been given full-thickness burn of20% TBSA was found to be inhibitory to the respiratory function of the mitochondria and theenergization of the inner membrane. The inhibition was also demonstrated by the 1: 2 and 1: 4and 1: 8 dilutions of the eschar extract. The extract of normal skin only showed weak inhibitory cf-fect. The 1: 4 dilution of normal skin had no (?) on mitochondrial functions. There is a widegap between the malonaldehyde (MDA) contents of these two kinds of extracts. An apparent posi-tive correlation was found between MDA content and degree of inhibition. However, on close ex-amination, it was found that the MDA content is not the direct cause of the decrease of therespiratory control ratio (RCR). The increase of MDA content is probably related to the destruc-tion of the lipid bilayer of the mitochondria. Data from the energization experiment show that theenergization process is compromised when the destruction of the membrane has proceeded to a cer-tain degrce. The inhibition of mitochondrial respiratory function becomes evident when encrgizationfunction is still normal. Therefore at least two mechanisms are operating in the inhibition process.Further work is needed to elucidate the problem.展开更多
Mitochondria were isolated from normal and cold adapted rat livers.The respiratory func-tion of mitochondria in rat livers,including ADP:O ratio(P/O)and the respiratory control ratio(RCR),was determined by oxygen elec...Mitochondria were isolated from normal and cold adapted rat livers.The respiratory func-tion of mitochondria in rat livers,including ADP:O ratio(P/O)and the respiratory control ratio(RCR),was determined by oxygen electrode method,The protein synthesis in mitochondria wasstudied by observing the incorporation of[~3H]-Leucine into mitochondria.Polyacrylamide gelelectrophoresis was carried out to detect the changes of the inner membrane proteins.It was shownthat the P/O and RCR decreased in cold adapted rats in the 2nd and 4th weeks and returned tothe control level in the 6th week,the protein synthesis of mitochondria decreased significantly incold adapted rats in 1,2 and 4 weeks;the electrophoretic pattern of the inner membrane proteinsin mitochondria from cold adapted rat livers revealed some new bands.展开更多
目的:观察长期耐力训练及限食对老龄大鼠骨骼肌线粒体功能的影响,比较其单独及协同作用,探讨耐力训练及限食的线粒体机制。方法:32只17月龄雄性SD大鼠分为4组:安静组(Control,C)、限食组(Caloric-Restricted,CR)、运动组(Exercise,E)和...目的:观察长期耐力训练及限食对老龄大鼠骨骼肌线粒体功能的影响,比较其单独及协同作用,探讨耐力训练及限食的线粒体机制。方法:32只17月龄雄性SD大鼠分为4组:安静组(Control,C)、限食组(Caloric-Restricted,CR)、运动组(Exercise,E)和限食加运动组(Caloric-restricted and Exercise,E+CR),训练方式为跑台运动,中等运动强度(64%VO2max,15m/min,60分钟/天,每周5天),限食摄入的标准为正常摄入组的60%,共训练及限食12周,相同月龄对照组正常饲养。12周后于末次训练后取大鼠骨骼肌进行线粒体呼吸功能测定。结果:态3呼吸CR组和CR+E组显著降低,E组明显增高(P<0.05);态4呼吸CR组显著降低(P<0.05),E组和CR+E无明显变化;呼吸控制比CR组和CR+E组显著降低,E组明显增高(P<0.05);ATP合成活力E组显著增加(P<0.05),而CR组和CR+E组无明显变化,结论:耐力训练改善了大鼠骨骼肌线粒体呼吸链的电子传递效率,提高了线粒体呼吸功能,增强了氧化磷酸化能力,限食对能量需求较小,ATP生成减少,在低消耗水平下对线粒体呼吸功能有着良性影响。展开更多
Objective To investigate the radiation effect on mitochondria of the mouse liver in vivo. Methods Livers of BALB/c mice were squeezed out through openings cut on the upper abdomen and radiation doses were delivered ...Objective To investigate the radiation effect on mitochondria of the mouse liver in vivo. Methods Livers of BALB/c mice were squeezed out through openings cut on the upper abdomen and radiation doses were delivered only to the livers. Radiation effects on the structure and function of liver mitochondria were evaluated from both respiratory control ratio (RCR) and ADP/O (adenosine 5' diphosphate/oxygen) ratio supported by two substrates, succinate and glutamate/malate mixtures.Results The data showed that both state 3 and state 4 respiratory rates were significantly decreased concomitantly with the increase of radiation dose up to 20 Gy. Succinate supported, but not glutamate/malate supported, respiratory control ratio, was also decreased with the increase of radiation dose. ADP/O ratios measured from the support of both succinate and glutamate/malate were not changed by γ radiation. This differential radiation damage indicated that respiratory control ratio was more vulnerable to radiation than oxidative phosphorylation.Conclusions Although the functional integrity of ATP synthetic machinery of the mitochondria was not perturbed by ionizing radiation under 20 Gy, the structural integrity of mitochondrial membrane might be affected by ionizing radiation at a dose dependent manner.展开更多
文摘Extract of the eschar tissue taken from the rat which had been given full-thickness burn of20% TBSA was found to be inhibitory to the respiratory function of the mitochondria and theenergization of the inner membrane. The inhibition was also demonstrated by the 1: 2 and 1: 4and 1: 8 dilutions of the eschar extract. The extract of normal skin only showed weak inhibitory cf-fect. The 1: 4 dilution of normal skin had no (?) on mitochondrial functions. There is a widegap between the malonaldehyde (MDA) contents of these two kinds of extracts. An apparent posi-tive correlation was found between MDA content and degree of inhibition. However, on close ex-amination, it was found that the MDA content is not the direct cause of the decrease of therespiratory control ratio (RCR). The increase of MDA content is probably related to the destruc-tion of the lipid bilayer of the mitochondria. Data from the energization experiment show that theenergization process is compromised when the destruction of the membrane has proceeded to a cer-tain degrce. The inhibition of mitochondrial respiratory function becomes evident when encrgizationfunction is still normal. Therefore at least two mechanisms are operating in the inhibition process.Further work is needed to elucidate the problem.
文摘Mitochondria were isolated from normal and cold adapted rat livers.The respiratory func-tion of mitochondria in rat livers,including ADP:O ratio(P/O)and the respiratory control ratio(RCR),was determined by oxygen electrode method,The protein synthesis in mitochondria wasstudied by observing the incorporation of[~3H]-Leucine into mitochondria.Polyacrylamide gelelectrophoresis was carried out to detect the changes of the inner membrane proteins.It was shownthat the P/O and RCR decreased in cold adapted rats in the 2nd and 4th weeks and returned tothe control level in the 6th week,the protein synthesis of mitochondria decreased significantly incold adapted rats in 1,2 and 4 weeks;the electrophoretic pattern of the inner membrane proteinsin mitochondria from cold adapted rat livers revealed some new bands.
文摘目的:观察长期耐力训练及限食对老龄大鼠骨骼肌线粒体功能的影响,比较其单独及协同作用,探讨耐力训练及限食的线粒体机制。方法:32只17月龄雄性SD大鼠分为4组:安静组(Control,C)、限食组(Caloric-Restricted,CR)、运动组(Exercise,E)和限食加运动组(Caloric-restricted and Exercise,E+CR),训练方式为跑台运动,中等运动强度(64%VO2max,15m/min,60分钟/天,每周5天),限食摄入的标准为正常摄入组的60%,共训练及限食12周,相同月龄对照组正常饲养。12周后于末次训练后取大鼠骨骼肌进行线粒体呼吸功能测定。结果:态3呼吸CR组和CR+E组显著降低,E组明显增高(P<0.05);态4呼吸CR组显著降低(P<0.05),E组和CR+E无明显变化;呼吸控制比CR组和CR+E组显著降低,E组明显增高(P<0.05);ATP合成活力E组显著增加(P<0.05),而CR组和CR+E组无明显变化,结论:耐力训练改善了大鼠骨骼肌线粒体呼吸链的电子传递效率,提高了线粒体呼吸功能,增强了氧化磷酸化能力,限食对能量需求较小,ATP生成减少,在低消耗水平下对线粒体呼吸功能有着良性影响。
文摘Objective To investigate the radiation effect on mitochondria of the mouse liver in vivo. Methods Livers of BALB/c mice were squeezed out through openings cut on the upper abdomen and radiation doses were delivered only to the livers. Radiation effects on the structure and function of liver mitochondria were evaluated from both respiratory control ratio (RCR) and ADP/O (adenosine 5' diphosphate/oxygen) ratio supported by two substrates, succinate and glutamate/malate mixtures.Results The data showed that both state 3 and state 4 respiratory rates were significantly decreased concomitantly with the increase of radiation dose up to 20 Gy. Succinate supported, but not glutamate/malate supported, respiratory control ratio, was also decreased with the increase of radiation dose. ADP/O ratios measured from the support of both succinate and glutamate/malate were not changed by γ radiation. This differential radiation damage indicated that respiratory control ratio was more vulnerable to radiation than oxidative phosphorylation.Conclusions Although the functional integrity of ATP synthetic machinery of the mitochondria was not perturbed by ionizing radiation under 20 Gy, the structural integrity of mitochondrial membrane might be affected by ionizing radiation at a dose dependent manner.
