Genetic similarities of 13 inbred lines of maize (Zea mays L.) were analyzed by restriction fragment length polymorphisms (RFLPs). The objectives of the study were to detect genetic similarities among 13 inbreds and t...Genetic similarities of 13 inbred lines of maize (Zea mays L.) were analyzed by restriction fragment length polymorphisms (RFLPs). The objectives of the study were to detect genetic similarities among 13 inbreds and to assign them to heterotic groups. By means of 24 probe_enzyme combinations (PECs) selected for locus specificity, clear patterns and reproducibility, 85 alleles were found with an average of 3.3 alleles per locus. The allelic frequency data were used to estimate genetic similarities among lines, and as a result the diversity index of 0.499 was obtained. Genetic similarities between the pairs of 13 lines ranged from 0.523 up to 0.802 with an average of 0.649. The UPGMA clustering algorithm analysis classified the 13 lines into five groups, which generally corresponded to known maize heterotic groups based on pedigree information. The authors concluded that RFLP_based markers could be used for investigating genetic relationships between maize inbred lines and assigning them to heterotic groups, but it seemed that a large number of PECs were needed to obtain reliable estimates of genetic similarity.展开更多
应用Southern印迹杂交方法和PCR新技术,对东北地区11个甲型血友病家系检测FⅧ基因内的Bcl Ⅰ和Xba Ⅰ位点的RFLPs和基因外Stl4/Taq I RFLPs,分析了FⅧ基因的连锁状况,7名肯定携带者中4名能提供多态信息,4名可疑携带者之中2名可确定为缺...应用Southern印迹杂交方法和PCR新技术,对东北地区11个甲型血友病家系检测FⅧ基因内的Bcl Ⅰ和Xba Ⅰ位点的RFLPs和基因外Stl4/Taq I RFLPs,分析了FⅧ基因的连锁状况,7名肯定携带者中4名能提供多态信息,4名可疑携带者之中2名可确定为缺陷FⅧ基因的携带者,1名可确定为正常FⅧ基因。为婚姻、生育指导和产前基因诊断提供依据。进一步明确了应用PCR技术联合进行多个位点的RFLPs连锁分析进行甲型血友病基因诊断的可行性及优越性。展开更多
文摘Genetic similarities of 13 inbred lines of maize (Zea mays L.) were analyzed by restriction fragment length polymorphisms (RFLPs). The objectives of the study were to detect genetic similarities among 13 inbreds and to assign them to heterotic groups. By means of 24 probe_enzyme combinations (PECs) selected for locus specificity, clear patterns and reproducibility, 85 alleles were found with an average of 3.3 alleles per locus. The allelic frequency data were used to estimate genetic similarities among lines, and as a result the diversity index of 0.499 was obtained. Genetic similarities between the pairs of 13 lines ranged from 0.523 up to 0.802 with an average of 0.649. The UPGMA clustering algorithm analysis classified the 13 lines into five groups, which generally corresponded to known maize heterotic groups based on pedigree information. The authors concluded that RFLP_based markers could be used for investigating genetic relationships between maize inbred lines and assigning them to heterotic groups, but it seemed that a large number of PECs were needed to obtain reliable estimates of genetic similarity.
基金This work was supported by the High-technology Research and Development Program of China (No. 2004AA211150) and Ph.D Education Foundation from the Ministry of Education of China (No. 20020504009).
文摘应用Southern印迹杂交方法和PCR新技术,对东北地区11个甲型血友病家系检测FⅧ基因内的Bcl Ⅰ和Xba Ⅰ位点的RFLPs和基因外Stl4/Taq I RFLPs,分析了FⅧ基因的连锁状况,7名肯定携带者中4名能提供多态信息,4名可疑携带者之中2名可确定为缺陷FⅧ基因的携带者,1名可确定为正常FⅧ基因。为婚姻、生育指导和产前基因诊断提供依据。进一步明确了应用PCR技术联合进行多个位点的RFLPs连锁分析进行甲型血友病基因诊断的可行性及优越性。
