The association of retinol binding protein 4 （RBP4） with atherosclerosis of the carotid artery in type 2 diabetes mellitus （T2DM） remains undefined. We aimed to investigate the correlation of RBP4 expression with ...The association of retinol binding protein 4 （RBP4） with atherosclerosis of the carotid artery in type 2 diabetes mellitus （T2DM） remains undefined. We aimed to investigate the correlation of RBP4 expression with atherosclerosis of the carotid artery in T2DM. A total of 1,076 subjects were investigated for intima-media thickness of the bilateral common carotid arteries, and they were divided into three groups： in group Ⅰ, patients had normal neck vascular ultra- sound, in group Ⅱ, intimal carotid artery media thickness was equal to or more than 1 mm, and in group Ⅲ, carotid artery plaque was present. Height, weight, blood pressure （BP）, fasting plasma glucose （FPG）, hemoglobin Alc （HbA1c）, total cholesterol （TC）, triglyceride （TG）, low-density lipoprotein cholesterol （LDL-C）, high-density lipopro- tein cholesterol （HDL-C）, apolipoprotein A-1 （apoA-1）, apolipoprotein B （apoB） and lipoprotein （a） [Lp（a）] were determined by routine laboratory methods. RBP4 and high sensitivity C reactive protein （HsCRP） were measured by an enzyme-linked immuno-sorbent assay, and insulin concentration was measured by an electrochemiluminescence sandwich immunoassay. Duration of diabetes, waist and BP, FPG, HbAlc, TG, TC, LDL-C, APOB, Lp（a）, HsCRP, RBP4 and homeostasis model assessment insulin resistance index （HOMA-IR） were significantly lower in group I than in the other two groups （P〈0.01, P〈0.01）. Plasma levels of HbAlc, RBP4, LDL-C, TC, HOMA-IR, HsCRP and Lp（a）, waist and BP were significantly increased in group III than in group II （P〈0.01）. Multivariate logistic regression analysis showed that there were seven factors associated with the occurrence of carotid artery atherosclero- sis and its risks in descending order were： high LDL-C, high waist, high HsCRP, duration of diabetes, high HOMA-IR, HbAlc and high RBP4. Our finding supported that RBP4 was positively correlated with carotid atherosclerosis in patients with T2DM and could be used as an early predictor of cardiovascular disease.展开更多
Objective To screen the proteins associated with four-and-a-half LIM domains 3(FHL3) 3' untranslated region(3'UTR) in glioma cells. Methods Western blot was adopted to detect the regulatory effect of poly(C)-b...Objective To screen the proteins associated with four-and-a-half LIM domains 3(FHL3) 3' untranslated region(3'UTR) in glioma cells. Methods Western blot was adopted to detect the regulatory effect of poly(C)-binding protein 2(PCBP2) on FHL3. Biotin pull-down and sliver staining were employed to screen and verify the candidate binding proteins of FHL3 3'UTR. Then liquid chromatography-tandem mass spectrometry(LC-MS/MS) and molecule annotation system were used to identify and analyze the candidate binding proteins. Immunoprecipitation was conducted to study the interaction between PCBP2 and polypyrimidine tract-binding protein 1(PTBP1), a binding protein identified by LC-MS/MS. Results PCBP2 could bind to FHL3 mRNA 3'UTR-A and inhibited the expression of FHL3 in T98 G glioms cells. 22 candidate binding proteins were identified. Among them, there were 11 RNA binding proteins, including PCBP2. PTBP1 associated with FHL3 mRNA 3'UTR and interacted with PCBP2 protein. Conclusion PCBP2 and PTBP1 can both associate with FHL3 mRNA 3'UTR through forming a protein complex.展开更多
目的探究血清前白蛋白(prealbumin,PA)和视黄醇结合蛋白(retinol-binding protein,RBP)在肝硬化及肝硬化合并肝癌患者中的临床价值。方法收集2022年1月至2022年9月在西南医科大学附属医院收治的428例肝硬化患者的临床资料。同时,根据Chi...目的探究血清前白蛋白(prealbumin,PA)和视黄醇结合蛋白(retinol-binding protein,RBP)在肝硬化及肝硬化合并肝癌患者中的临床价值。方法收集2022年1月至2022年9月在西南医科大学附属医院收治的428例肝硬化患者的临床资料。同时,根据Child-Pugh分级、终末期肝病模型(model for end stage liver disease,MELD)评分和本次入院是否合并肝癌对肝硬化患者进行分组,测定患者血清PA和RBP的水平,分析两项指标与肝硬化患者肝脏储备功能及肝硬化合并肝癌的相关性。结果Child-Pugh-A级患者的血清PA和RBP水平最高,Child-Pugh-C级最低,随着Child-Pugh分级的升高,血清PA和RBP水平逐渐降低(P<0.05);低MELD组患者血清PA和RBP水平显著高于高MELD组(P<0.05);肝硬化合并肝癌组患者的血清PA和RBP水平显著高于非肝癌组(P<0.05);进一步采用Spearman相关检验分析,血清PA、RBP水平与肝硬化患者Child-Pugh分级和MELD评分呈负相关(r<0,P<0.05),血清PA水平与肝癌的发生也呈负相关(r<0,P<0.05)。结论血清PA和RBP能够灵敏地反映肝功能损伤情况,评估肝硬化患者的残余肝细胞功能,并且PA在一定程度上能够预测肝癌的发生。展开更多
文摘The association of retinol binding protein 4 （RBP4） with atherosclerosis of the carotid artery in type 2 diabetes mellitus （T2DM） remains undefined. We aimed to investigate the correlation of RBP4 expression with atherosclerosis of the carotid artery in T2DM. A total of 1,076 subjects were investigated for intima-media thickness of the bilateral common carotid arteries, and they were divided into three groups： in group Ⅰ, patients had normal neck vascular ultra- sound, in group Ⅱ, intimal carotid artery media thickness was equal to or more than 1 mm, and in group Ⅲ, carotid artery plaque was present. Height, weight, blood pressure （BP）, fasting plasma glucose （FPG）, hemoglobin Alc （HbA1c）, total cholesterol （TC）, triglyceride （TG）, low-density lipoprotein cholesterol （LDL-C）, high-density lipopro- tein cholesterol （HDL-C）, apolipoprotein A-1 （apoA-1）, apolipoprotein B （apoB） and lipoprotein （a） [Lp（a）] were determined by routine laboratory methods. RBP4 and high sensitivity C reactive protein （HsCRP） were measured by an enzyme-linked immuno-sorbent assay, and insulin concentration was measured by an electrochemiluminescence sandwich immunoassay. Duration of diabetes, waist and BP, FPG, HbAlc, TG, TC, LDL-C, APOB, Lp（a）, HsCRP, RBP4 and homeostasis model assessment insulin resistance index （HOMA-IR） were significantly lower in group I than in the other two groups （P〈0.01, P〈0.01）. Plasma levels of HbAlc, RBP4, LDL-C, TC, HOMA-IR, HsCRP and Lp（a）, waist and BP were significantly increased in group III than in group II （P〈0.01）. Multivariate logistic regression analysis showed that there were seven factors associated with the occurrence of carotid artery atherosclero- sis and its risks in descending order were： high LDL-C, high waist, high HsCRP, duration of diabetes, high HOMA-IR, HbAlc and high RBP4. Our finding supported that RBP4 was positively correlated with carotid atherosclerosis in patients with T2DM and could be used as an early predictor of cardiovascular disease.
基金Supported by Peking Union Medical College Youth Fundthe Fundamental Research Funds for the Central Universities(3332013052)
文摘Objective To screen the proteins associated with four-and-a-half LIM domains 3(FHL3) 3' untranslated region(3'UTR) in glioma cells. Methods Western blot was adopted to detect the regulatory effect of poly(C)-binding protein 2(PCBP2) on FHL3. Biotin pull-down and sliver staining were employed to screen and verify the candidate binding proteins of FHL3 3'UTR. Then liquid chromatography-tandem mass spectrometry(LC-MS/MS) and molecule annotation system were used to identify and analyze the candidate binding proteins. Immunoprecipitation was conducted to study the interaction between PCBP2 and polypyrimidine tract-binding protein 1(PTBP1), a binding protein identified by LC-MS/MS. Results PCBP2 could bind to FHL3 mRNA 3'UTR-A and inhibited the expression of FHL3 in T98 G glioms cells. 22 candidate binding proteins were identified. Among them, there were 11 RNA binding proteins, including PCBP2. PTBP1 associated with FHL3 mRNA 3'UTR and interacted with PCBP2 protein. Conclusion PCBP2 and PTBP1 can both associate with FHL3 mRNA 3'UTR through forming a protein complex.
文摘目的探究血清前白蛋白(prealbumin,PA)和视黄醇结合蛋白(retinol-binding protein,RBP)在肝硬化及肝硬化合并肝癌患者中的临床价值。方法收集2022年1月至2022年9月在西南医科大学附属医院收治的428例肝硬化患者的临床资料。同时,根据Child-Pugh分级、终末期肝病模型(model for end stage liver disease,MELD)评分和本次入院是否合并肝癌对肝硬化患者进行分组,测定患者血清PA和RBP的水平,分析两项指标与肝硬化患者肝脏储备功能及肝硬化合并肝癌的相关性。结果Child-Pugh-A级患者的血清PA和RBP水平最高,Child-Pugh-C级最低,随着Child-Pugh分级的升高,血清PA和RBP水平逐渐降低(P<0.05);低MELD组患者血清PA和RBP水平显著高于高MELD组(P<0.05);肝硬化合并肝癌组患者的血清PA和RBP水平显著高于非肝癌组(P<0.05);进一步采用Spearman相关检验分析,血清PA、RBP水平与肝硬化患者Child-Pugh分级和MELD评分呈负相关(r<0,P<0.05),血清PA水平与肝癌的发生也呈负相关(r<0,P<0.05)。结论血清PA和RBP能够灵敏地反映肝功能损伤情况,评估肝硬化患者的残余肝细胞功能,并且PA在一定程度上能够预测肝癌的发生。
