[Objectives] This study was conducted to analyze the infection ability of Rhizobium radiobacter from Rosa chinensis to peach tree,so as to provide a theoretical basis for the identification and control of crown gall o...[Objectives] This study was conducted to analyze the infection ability of Rhizobium radiobacter from Rosa chinensis to peach tree,so as to provide a theoretical basis for the identification and control of crown gall of peach. [Methods]With peach variety ＂ Chiyohime＂ as an experimental material,R. radiobacter GOU1 was extracted from crown gall tissue of R. chinensis susceptible to crown gall,and its infection ability to peach tree was studied by in-vitro dip method.[Results]Stem segments of peach tree were inoculated with R. radiobacter GOU1 by in-vitro dip method. Calli grew rapidly after inoculation,and 28 d later,the infection rate of R. radiobacter GOU1 was 50%,indicating that R. radiobacter GOU1 could infect peach tree easier. The infection on stem segments of peach tree could be detected in the fourth week by in-vitro dip method. Culturing peach stem segments in MS medium containing 10 μM/L NAA and 10 μM/L BAP in vitro is beneficial to survival and could promote growth of callus. [Conclusions]The detection rate of R. radiobacter GOU1 infection on stem segments of peach tree did not change from the fourth week to the sixth week. Therefore,in future studies,the detection of R. radiobacter infection on inoculated stem segments of peach tree could be performed after 4 weeks of culture rather than 6 weeks of culture.展开更多
为获得固氮促生能力强、寄主识别范围广的高效抗逆葛藤根瘤菌。本试验收集贵州省16个县(市)5个不同生态功能区的葛藤根瘤样品,经分离纯化、16 S rRNA基因序列比对、区系分析以及百脉根盆栽回接筛选获得高效抗逆葛藤根瘤菌。结果表明,分...为获得固氮促生能力强、寄主识别范围广的高效抗逆葛藤根瘤菌。本试验收集贵州省16个县(市)5个不同生态功能区的葛藤根瘤样品,经分离纯化、16 S rRNA基因序列比对、区系分析以及百脉根盆栽回接筛选获得高效抗逆葛藤根瘤菌。结果表明,分离纯化获得39株根瘤菌分属于7属17种,区系分析优势属为慢生根瘤菌属(Bradyrhizobium)和假单胞菌属(Pseudomonas),分布频率均高达24.39%;优势种为根癌土壤杆菌(Agrobacterium tumefaciens)和豆根瘤菌(Rhizobium leguminosarum),发生频率相对较高,分别达14.63%和12.20%。盆栽回接筛选获得14株优良促生固氮葛藤根瘤菌,其中分离自荔波县驾欧乡的LJPR 58-1与宿主植物的共生结瘤及促生长率均与另外13个优良菌株差异显著(p<0.05),为最优固氮根瘤菌。展开更多
基金Supported by Natural Science Foundation of China(31260461)Guangxi Innovation Team Nanning Test Station Project of National Modern Agriculture Technology System(nycytxcxtd-03-12-3,nycytxcxtd-04-18)Undergraduate Teaching Reform Project of Higher Education of Guangxi Province(2017JGB132)
文摘[Objectives] This study was conducted to analyze the infection ability of Rhizobium radiobacter from Rosa chinensis to peach tree,so as to provide a theoretical basis for the identification and control of crown gall of peach. [Methods]With peach variety ＂ Chiyohime＂ as an experimental material,R. radiobacter GOU1 was extracted from crown gall tissue of R. chinensis susceptible to crown gall,and its infection ability to peach tree was studied by in-vitro dip method.[Results]Stem segments of peach tree were inoculated with R. radiobacter GOU1 by in-vitro dip method. Calli grew rapidly after inoculation,and 28 d later,the infection rate of R. radiobacter GOU1 was 50%,indicating that R. radiobacter GOU1 could infect peach tree easier. The infection on stem segments of peach tree could be detected in the fourth week by in-vitro dip method. Culturing peach stem segments in MS medium containing 10 μM/L NAA and 10 μM/L BAP in vitro is beneficial to survival and could promote growth of callus. [Conclusions]The detection rate of R. radiobacter GOU1 infection on stem segments of peach tree did not change from the fourth week to the sixth week. Therefore,in future studies,the detection of R. radiobacter infection on inoculated stem segments of peach tree could be performed after 4 weeks of culture rather than 6 weeks of culture.
