In this study, an （AG） n microsatellite-enriched library of Rhododendron simsii Planch. was constructed with FIASCO （ fast Isolation by AFLP sequences containing repeats） method. Among 356 positive colonies verifi...In this study, an （AG） n microsatellite-enriched library of Rhododendron simsii Planch. was constructed with FIASCO （ fast Isolation by AFLP sequences containing repeats） method. Among 356 positive colonies verified by colony PCR of the （AG）n microsatellite-enriched library, 233 colonies were selected for further sequencing. According to the results, 202 colonies containing microsatellites were obtained, accounting for 86.7%. Discarding sequences with miscella- neous peaks, overlapping peaks and weak signals, 39 sequences were screened finally for subsequent primer exploitation, including 22 perfect type SSR loci, 5 im- perfect type SSR loci and 12 compound type SSR loci. The FIASCO method is efficient and feasible for constructing microsatellite-enriched libraries of Rhododendron spp. , which laid a solid foundation for isolation of microsatellite loci and investigation of genetic diversity and genetic structure of Rhododendron spp.展开更多
This study was conducted to investigate the dynamic changes of several hormones in Rhododendron simsii floral organs to provide theoretical basis for controlling its flowering period. A R. simsii variety ＂Purple Cran...This study was conducted to investigate the dynamic changes of several hormones in Rhododendron simsii floral organs to provide theoretical basis for controlling its flowering period. A R. simsii variety ＂Purple Crane＂ was selected as the experimental material, and its flower organs were sampled at nine floral developmental stages to measure the contents of gibberellic acid-3 （GA3 ）, abscisic acid （ABA）, indole-3-acetic acid （IAA）, and zeatin riboside （ZR） through enzyme-linked immunosorbent assay （ELISA）. The results showed that the content of ZR was highest at full flowering stage; IAA and GA3 contents were higher than other stages in the process of flower bud differentiation and flowering. ABA played an important role in the regulation of flower bud formation. The results will provide theoretical references for exploring the regulation of plant hormones towards Rhododendron flower traits and breeding new varieties.展开更多
基金Supported by Doctoral Starting up Foundation of Huanggang Normal University(2013030903)Opening Fund of Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resource Comprehensive Utilization(2013000403)
文摘In this study, an （AG） n microsatellite-enriched library of Rhododendron simsii Planch. was constructed with FIASCO （ fast Isolation by AFLP sequences containing repeats） method. Among 356 positive colonies verified by colony PCR of the （AG）n microsatellite-enriched library, 233 colonies were selected for further sequencing. According to the results, 202 colonies containing microsatellites were obtained, accounting for 86.7%. Discarding sequences with miscella- neous peaks, overlapping peaks and weak signals, 39 sequences were screened finally for subsequent primer exploitation, including 22 perfect type SSR loci, 5 im- perfect type SSR loci and 12 compound type SSR loci. The FIASCO method is efficient and feasible for constructing microsatellite-enriched libraries of Rhododendron spp. , which laid a solid foundation for isolation of microsatellite loci and investigation of genetic diversity and genetic structure of Rhododendron spp.
基金Supported by the Scientific and Technological Research Program of Department of Science and Technology of Hubei Province(Q20152903)
文摘This study was conducted to investigate the dynamic changes of several hormones in Rhododendron simsii floral organs to provide theoretical basis for controlling its flowering period. A R. simsii variety ＂Purple Crane＂ was selected as the experimental material, and its flower organs were sampled at nine floral developmental stages to measure the contents of gibberellic acid-3 （GA3 ）, abscisic acid （ABA）, indole-3-acetic acid （IAA）, and zeatin riboside （ZR） through enzyme-linked immunosorbent assay （ELISA）. The results showed that the content of ZR was highest at full flowering stage; IAA and GA3 contents were higher than other stages in the process of flower bud differentiation and flowering. ABA played an important role in the regulation of flower bud formation. The results will provide theoretical references for exploring the regulation of plant hormones towards Rhododendron flower traits and breeding new varieties.
