青杄PwRhomboid基因表达特性及其抗旱作用

通过对青杄转录组测序分析,筛选出干旱响应基因PwRhomboid,分析该基因在耐旱方面的功能特性,为揭示青杄的耐旱机理提供理论基础。采用生物信息学对PwRhomboid的氨基酸序列和同源基因进行比对;利用RT-qPCR技术分析PwRhomboid对不同非生物胁迫和激素的响应情况;通过瞬时转化烟草叶片检测PwRhomboid蛋白的亚细胞定位;获得拟南芥和马铃薯的PwRhomboid转基因株系并进行干旱表型验证。研究结果显示,PwRhomboid的氨基酸序列在N端与其同源物种的氨基酸序列具有较大差异,在C端相似度较高,保守性较好;其蛋白主要定位于细胞膜。PwRhomboid的表达量经干旱、低温和脱落酸诱导后发生有显著变化,在成熟叶中表达量最高,其能够提高转基因拟南芥和马铃薯的耐旱性。经干旱处理后,与对照组拟南芥野生型和pCM1205株系相比,PwRhomboid过表达株系PwRhomboid-L1和PwRhomboid-L2的存活率更高,叶绿素荧光最大光化学效率和实际光化学效率更高;在经聚乙二醇模拟干旱处理后,与野生型相比,过表达株系PwRhomboid-L3、PwRhomboid-L4的马铃薯苗高更高。结果表明PwRhomboid的表达量会受到干旱等逆境和激素的影响,过表达PwRhomboid能够提高拟南芥和马铃薯的耐旱性。

Evaluation of the versatility of perforators-enhanced rhomboid flap technique for ischial sores reconstruction

Background: Prolonged pressure on the skin known as pressure sores, can cause frequent injuries to the skin and underlying tissues. However, several prevention approaches, including non-surgical and surgical management,are available. In flap surgery for pressure sores, a variety of flap types may be used;each offers certain benefits and some incidences of complications and recurrence. This study evaluated the versatility of the perforatorenhanced rhomboid flap technique for ischial sore construction.Methods: This prospective study was conducted in the Plastic Surgery Department of Fayoum University Hospital.The perforator-enhanced rhomboid flap technique was employed in 20 patients with ischial pressure sores who failed to respond to conservative treatment. A proper history was obtained and a preoperative arterial duplex was performed to determine the site of the prominent perforators for their preservation at the base of the flap.Postoperative follow-ups and evaluations were performed.Results: The mean age of the study group was 43.7 ± 13.1, ranging from 19 to 65 years. Moreover, 80% of the participants were males while 20% were female. The main etiology of the sores was paraplegia in 45% of cases.The mean surface area of sores was 24.8 ± 6.3 cm2. In 75% of cases, the color match of the flap was rated as excellent. Similarly, 70% of the patients rated flap thickness as excellent. In 55% of the cases, the scar appearance was deemed excellent. Overall satisfaction was reported as excellent by 40% of the patients, while only 5% reported poor satisfaction. Approximately, 10% of the cases were identified with seroma, and 30% had wound dehiscence of a mean size of 1.33 ± 0.98 cm and required around 6.8 ± 1.9 weeks to heal.Conclusion: The perforator-enhanced rhomboid flap technique is a versatile method for reconstructing ischial pressure sores owing to its technical ease, short operative time, reliable vascularity, aesthetic outcome, matching skin color, and short recovery time. The main limitations include large defects with large surface areas, lack of a nearby donor site for a rhomboid flap, and scarring from prior procedures around the sore.

柔嫩艾美耳球虫rhomboid抗原基因在卡介苗中的克隆与表达

以含柔嫩艾美耳球虫rhomboid基因的重组质粒为模板,应用PCR方法扩增该基因完整开放阅读框,克隆至pMD18-T载体中。分别用限制性内切酶PstⅠ/ClaⅠ和PvuⅡ/ClaⅠ进行双酶切,将rhomboid目的基因克隆到大肠杆菌-分支杆菌穿梭表达载体pMV261和整合表达载体pMV361中,获得重组质粒pRho-261和pRho-361。将重组质粒电穿孔转化卡介苗,经热诱导后对其表达产物进行SDS-PAGE和western blot分析。结果表明成功构建了两个重组质粒,其表达产物与柔嫩艾美耳球虫阳性血清具有免疫反应性,为重组卡介苗在鸡球虫病防治方面的研究奠定了基础。

斯氏艾美耳球虫Rhomboid基因抗原优势区的筛选与鉴定

为了筛选并鉴定斯氏艾美耳球虫Rhomboid基因抗原优势区,试验采用预测斯氏艾美耳球虫Rhomboid基因跨膜结构域,PCR扩增4段目的片段,构建表达载体,并通过SDS-PAGE和Westernblot技术分析目的蛋白。结果表明:成功克隆出4段斯氏艾美耳球虫Rhomboid基因,片段大小分别为102 bp、75 bp、93 bp、99 bp,重组原核表达质粒p ET-32a-Rho1~4构建成功;经SDS-PAGE和Western-blot分析,构建的4段重组质粒都得到了表达且与理论值相符,且重组蛋白中p ET-32aRho4可与兔斯氏艾美耳球虫阳性血清发生特异性反应。

柔嫩艾美耳球虫F2杂交株Rhomboid基因在大肠杆菌中的表达

根据ET-Rhomboid基因开放阅读框,插入有利于表达的酶切位点设计引物,以虫体DNA为模板扩增并克隆了ET-Rhomboid基因,并将该基因与PET-28a(+)质粒载体连接,构建成了原核表达载体PET-ET-Rhom-boid。将构建好的表达质粒转入大肠杆菌DE3,经IPTG诱导表达后,用SDS-PAGE检测表达产物,用蛋白印迹(Western blotting)检测其反应原性。结果表明:含重组质粒的工程菌有明显的表达产物为31 kD的融合蛋白,与推测的融合蛋白的分子量吻合,蛋白印迹检测具有反应原性。

Effect of layer thickness on the flexural property and microstructure of 3D-printed rhomboid polymer-reinforced cemented tailing composites

For mines with poor ore bodies and surrounding rocks,the general mining method does not allow the ore to be extracted from underground safely and efficiently.For these mines,the downward layered filling mining technique is undoubtedly the most suitable mining method.The downward filling mining technique may eliminate the troubles relating to poor ore deposit conditions,such as production safety,ore loss rate,and depletion rate.However,in this technique,the safety of the artificial roof of the next stratum is of paramount importance.Cementitious tailings backfilling(CTB)that is not sufficiently cemented and causes collapses could threaten ore production.This paper explores a diamond-shaped composite structure to mimic the stability of a glued false roof in an actual infill mine based on the recently emerged three-dimensional(3D)printing technology.Experimental means such as three-point bending and digital image correlation(DIC)techniques were used to explore the flexural characteristics of 3D construction specimens and CTB combinations with different cement/tailings weight ratios at diverse layer heights.The results show that the 3D structure with a 14-mm ply height and CTB has strong flexural characteristics,with a maximum deflection value of 30.1 mm,while the 3D-printed rhomboid polymer(3D-PRP)structure with a 26-mm ply height is slightly worse in terms of flexural strength characteristics,but it has a higher maximum flexural strength of 2.83 MPa.A combination of 3D structure and CTB has more unique mechanical properties than CTB itself.This research work offers practical knowledge on the artificial roof performance of the downward layered filling mining technique and builds a scientific knowledge base regarding the successful application of CTB material in mines.

OTOPOINT PRESSURE THERAPY FOR TREATMENT OF 32 CASES OF STRAIN OF RHOMBOID MUSCLE

We adopted auricular pressure therapy to treat 32 cases with strain of rhomboidmuscle and achieved satisfactory results,namely,the cure rate was 46.87％,improved rate 40.67％,and the total effective rate 87.54％.

Rhomboid Flap vs. Keystone Perforator Island Flap (KPIF) in the Treatment of Pilonidal Sinus Disease: Comparison of Short-Term Results

Background: Pilonidal disease is a chronic inflammatory disease of the sacrococcygeal region that mainly affects young people. Its incidence is 26 cases per 100,000 persons. Although many techniques have been described, there is no consensus on the treatment of pilonidal sinus disease (PSD). Materials and Methods: This study included 30 patients with PSD who were treated between May 2014 and September 2017. All cases underwent excision and flap reconstruction. The operative time, postoperative complications, the length of hospital stay, painless sitting and walking time, patient satisfaction and recurrence were evaluated prospectively. Results: The results of this prospective, randomized and comparative study are based on experience of a single surgical centre. All patients were followed up 18 months after discharge from the hospital. There is a difference in surgery durations (minutes) between the two groups (33.86 ± 2.89 min. in “keystone” flap vs. 41.26 ± 4.19 in the “rhombic” flap group) (p = 0.001). There were no significant differences in the length of hospital stay, painless sitting and walking time or patient satisfaction. The total complication rate was 66.6% after rhomboid flap compared with 6.6% after keystone flap. There was no flap necrosis. Conclusions: Both of these methods have shown to be successful in treatment of PSD. The KPIF is associated with the advantages of very simple design, abundant blood supply from the perforator vessels and lower rate of complication.

鸡堆型艾美耳球虫Rhomboid蛋白的原核表达及其多克隆抗体的制备和鉴定

为了研究堆型艾美耳球虫Rhomboid蛋白的生物学功能及其作为潜在免疫抗原蛋白的可能性,根据Gen Bank上柔嫩艾美耳球虫Rhomboid基因序列(DQ323509.1)设计特异性引物,以堆型艾美耳球虫c DNA为模板PCR扩增包含Rhomboid基因ORF的片段。根据测序结果及抗原表位分析,设计表达区域特异性引物,PCR扩增后连接于p GEX-6P-1原核表达载体成功构建p GEX-Rhomboid表达质粒。将此质粒转入大肠杆菌Rosetta感受态细胞中进行IPTG诱导表达,SDS-PAGE分析显示有36 ku的预期蛋白表达。蛋白纯化复性后免疫新西兰大白兔制备兔抗Rhomboid多克隆抗体。ELISA方法检测该多克隆抗体效价达到216,Western-blot及瞬时转染pc DNA-Rhomboid质粒表明,制备的多克隆抗体与堆型艾美耳球虫子孢子目的蛋白和真核质粒瞬时转染BHK21细胞表达蛋白均具有良好的反应性。本试验结果为进一步研究堆型艾美耳球虫Rhomboid蛋白的生物学活性奠定了基础。

弓形虫Rhomboid-IL-2基因重组卡介苗的构建及其对犬的免疫效果

目的构建弓形虫Rhomboid以及犬白介素2基因重组卡介苗,并观察其对免疫犬的免疫效果。方法将鉴定正确的弓形虫Rhombiod基因和犬IL-2基因一起克隆到大肠埃希菌-分枝杆菌穿梭表达载体和整合表达载体中,构建重组质粒pMV261(361)-IL2-Rho。将重组质粒转入卡介苗中,对表达产物进行SDS-PAGE电泳分析及Western blot鉴定。分别用重组卡介苗pMV261(361)-IL2-Rho和pMV261(361)-Rho经皮下免疫犬,检测免疫犬体液及细胞免疫水平变化并观察其存活时间。结果成功构建pMV261(361)-IL2-Rho重组质粒,弓形虫Rhomboid基因在重组卡介苗中稳定表达46ku的目的蛋白,Western blot显示该蛋白能被弓形虫感染鼠血清识别。动物实验表明,rBCGpMV261(361)-IL2-Rho能诱导产生较强的体液免疫和Th1型细胞免疫应答反应,rBCGpMV361-IL2-Rho免疫组感染弓形虫后的存活时间组为(14.40±4.50)d,rBCGpMV261-IL2-Rho免疫组感染弓形虫后的存活时间组为(13.00±4.75)d,较BCG对照组存活时间(8.20±5.45)d有所延长。结论 rBCGpMV261(361)-IL2-Rho蛋白具有免疫原性。该重组卡介苗对犬弓形虫感染有一定保护效果。

斯氏艾美耳球虫兰州株Rhomboid基因的克隆及原核表达

目的克隆并原核表达斯氏艾美耳球虫(Eimeria stiedai,E.stiedai)兰州株Rhomboid基因。方法通过分析基因保守区设计引物,采用RT-PCR方法与3′RACE技术相结合,扩增获得兔斯氏艾美耳球虫Rhomboid基因cDNA全序列,并对其进行序列分析;将获得的Rhomboid基因克隆至原核表达载体pET-28a中,转化E.coli Rosetta(DE3),IPTG诱导表达,并对表达产物进行SDS-PAGE及Western blot分析。结果 Rhomboid基因开放阅读框全长774 bp,编码257个氨基酸残基,预测蛋白质相对分子质量和等电点分别为28 120和8.64,与鸡柔嫩艾美耳球虫(E.tellan)Rhomboid蛋白氨基酸序列的同源性为84.44%;构建的重组原核表达质粒pET-Rhomboid经双酶切和测序证实构建正确;表达的重组蛋白相对分子质量约为29 000,并可与兔抗E.stiedai阳性血清发生特异性反应。结论成功克隆并表达了Rhomboid基因,为其生物学功能及以其作为斯氏艾美耳球虫疫苗候选基因的研究奠定了基础。

弓形虫Rhomboid-1蛋白基因的克隆及原核表达

目的克隆并原核表达刚地弓形虫(Toxoplasma gondii)Rhomboid-1(TgROM1)蛋白。方法收集、纯化弓形虫速殖子,用Trizol法提取总RNA,应用RT-PCR技术扩增TgROM1基因,回收的PCR产物与pMD18-T载体连接,构建重组克隆质粒pMD18-T-TgROM1。将重组克隆质粒亚克隆至原核表达载体pGEX-4T-1中,构建重组表达质粒pGEX-4T-1-TgROM1并转化至Rosetta感受态,用IPTG诱导表达,表达产物进行SDS-PAGE和Western blot分析。结果成功克隆了643bp的TgROM1基因,双酶切鉴定重组表达质粒pGEX-4T-1-TgROM1构建正确。SDS-PAGE检测重组表达质粒表达的TgROM1蛋白分子质量约为48ku,Western blot检测表明该蛋白能被鼠抗弓形虫血清识别。结论成功克隆了弓形虫ROM1基因并原核表达了具有反应原性的重组TgROM1蛋白,为该蛋白的功能研究奠定了基础。

微小隐孢子虫与安氏隐孢子虫Rhomboid基因部分序列的克隆和分析

目的获得我国微小隐孢子虫长春株与安氏隐孢子虫长春株Rhomboid基因(CPRho和CARho)部分序列并确定与国外分离株相应序列的同源性。方法应用RT-PCR技术扩增微小隐孢子虫长春株与安氏隐孢子虫长春株Rhomboid基因片段,然后将其克隆到pMD18-T载体中,测得序列应用DNAMAN软件进行序列分析。结果经RT-PCR扩增出微小隐孢子虫长春株与安氏隐孢子虫长春株Rhomboid基因部分序列,大小均为723 bp。经DNA MAN软件分析,表明我国微小隐孢子虫长春株与安氏隐孢子虫长春株Rhomboid核苷酸序列同源性为99.6%,氨基酸同源性为99.2%。二者与GenBank公布的相应序列核苷酸同源性分别为99.9%和99.7%,而氨基酸同源性均为99.6%。结论获得了微小隐孢子虫长春株与安氏隐孢子虫长春株Rhomboid基因部分序列,为继续研究该基因的侵入功能等奠定了基础。

贝氏隐孢子虫Rhomboid基因的克隆与生物信息学分析

将Gen Bank中发表的C.parvum、C.hominis和C.muris的菱形体蛋白(Rhomboid,ROM)基因序列进行比对分析,设计合成该基因保守片段的1对特异性引物,以贝氏隐孢子虫基因组DNA为模板,PCR扩增出CbROM基因保守片段,采用染色体步移技术获得基因片段3'端和5'端核苷酸序列,并对所得序列进行测序、拼接与生物信息学分析。结果显示,CbROM基因ORF长度为1 422 bp,编码473个氨基酸,蛋白质理论相对分子质量为52 700,等电点为8.65,是疏水性蛋白质,含有7个串连的跨膜螺旋区,无信号肽;该基因含有典型的菱形体蛋白相关保守结构域,属于菱形体蛋白酶家族;功能位点分析结果显示,CbROM蛋白序列包含6个N-糖基化位点,2个c AMP、c GMP依赖性蛋白激酶磷酸化位点,8个蛋白激酶C磷酸化位点,7个酪蛋白激酶II磷酸化位点和10个N-豆蔻酰化位点;二级结构中包含α-螺旋38.27%,β-折叠16.7%和无规则卷曲45.03%。本试验获得的贝氏隐孢子虫的Rhomboid基因序列,为该基因功能的进一步研究及其基因工程疫苗的研制奠定基础。

Self-assembly of Pyrene-modified Rhomboidal Metalloden- drimers via Directional Metal-ligand Bonding Approach

The design and self-assembly of pyrene-modified rhomboidal metallodendrimers R1--R6 via directional metal-ligand bonding approach is described. By employing pyrene-containing 120° di-Pt（II） acceptor and appropri- ate 60° dendritic dipyridyl donors, a variety of [G-1]--[G-3] pyrene-modified rhomboidal metallodendrimers with well-defined shape and size were prepared under mild conditions in high yields. The supramolecular dendrimers were characterized with multinuclear NMR （1H and 31p） and mass spectrometry （CSI-TOF-MS）. lsotopically re- solved mass spectrometry data support the existence of the pyrene-modified dendrimers with rhomboidal cavities, and NMR data were consistent with the formation of all ensembles. The shape and size of all rhomboidal den- drimers were investigated with the PM6 semiempirical molecular orbital method. Their primary photochemical properties were studied as well.

A new family of supramolecular multiferrocenyl rhomboids:Synthesis,characterization,and their electrochemical behavior

Two novel,supramolecular,multiferrocenyl rhomboids 5 and 6 have been successfully constructed from newly designed 60 ferrocenyl donor precursor 1 via coordination-driven self-assembly.The structures of all multiferrocenyl rhomboids were characterized by multinuclear NMR（~1H and ^（31）P）,CSI-TOF-MS, and PM6 semi-empirical molecular simulation,and their electrochemical behaviors have been investigated.

丘脑背侧中线和内侧核团参与痛信息传递与调控的神经环路机制研究进展

疼痛严重影响着患者的生活质量与身心健康,并可能引发或加剧焦虑、抑郁等情绪问题,但疼痛所涉及的复杂神经环路及其作用机制仍是一个亟待解决的科学问题。痛信息在中枢内的传递主要依赖于丘脑外侧系统和内侧系统。其中,来自脊髓或脑干的痛信息在经臂旁核(PBN)中继后,能够通过丘脑内侧系统的背侧中线和内侧核团(TMMN)进一步上传到大脑边缘皮质和皮质下结构。TMMN包括丘脑室旁核(PVT)、丘脑中央内侧核(CM)、丘脑背内侧核(MD)、丘脑连接核(Re)和丘脑菱形核(Rh),通过与前额叶皮质(PFC)、岛叶(IC)、杏仁核簇(AMY)等核团的联系参与痛信息及相关负性情绪的传递与调控。本综述总结了TMMN各核团在参与痛觉感受和情绪调节过程中神经元活性的变化以及相关神经通路和机制,以期为疼痛调控神经机制后续研究提供参考。

菱形肌联合前锯肌平面阻滞对肺叶切除患者镇痛效果的影响

目的探讨菱形肌联合前锯肌平面阻滞(RISS)对肺叶切除手术患者镇痛效果的影响。方法采用前瞻性研究方式,抽样选取2020年10月至2022年8月于我院行胸腔镜肺叶切除术治疗的82例患者作为研究对象,按照随机数字表法及双盲方式分为观察组(n=41)与对照组(n=41),观察组予以RISS阻滞联合静脉镇痛,对照组接受常规静脉镇痛,比较两组患者首次按压自控镇痛泵(PCIA)时间、48 h内PCIA有效按压次数、视觉模拟疼痛评分(VAS)、皮质醇(Cor)、去甲肾上腺素(NE)、CD3^(+)、CD4^(+)、CD8^(+)。结果观察组48 h内PCIA有效按压次数[(6.25±1.78)vs.(7.59±2.45)]次低于对照组,而首次按压PCIA时间[(12.85±2.85)h vs.(10.25±1.78 h)]长于对照组(t=2.274,、2.833、4.955,P<0.05)。重复测量方差分析显示,两组VAS评分、Cor、NE、CD3^(+)、CD4^(+)、CD8^(+)存在时间、组间及时间与组间交互作用,差异有统计学意义(P<0.05);在术后2、6、12、24、72 h时点,观察组的VAS评分均低于对照组(1.15±0.14)vs.(2.45±0.39)分、(2.05±0.31)vs.(3.42±0.59)分、(2.45±0.52)vs.(3.96±0.76)分、(2.11±0.35)vs.(3.02±0.57)分、(1.45±0.19)vs.(2.49±0.37)分(t=20.089、13.162、10.500、8.711、16.010,P<0.05);在术毕、术后24 h、72 h时点,观察组的Cor、NE均低于对照组(P<0.05)。在术后1、2 d,观察组的CD3^(+)、CD4^(+)高于对照组,而CD8^(+)低于对照组(P<0.05)。结论RISS用于胸腔镜肺叶切除术有利于增强镇痛效果,并减轻患者应激反应及减小对免疫功能的影响。

超声引导菱形肌-肋间肌阻滞和TPVB在电视胸腔镜手术术后早期疼痛控制中的应用比较

目的 比较超声引导下菱形肌-肋间肌阻滞和胸椎旁神经阻滞(TPVB)对电视胸腔镜手术患者术后早期疼痛的控制效果。方法 前瞻性选取2022年1月至2023年7月秦皇岛市第一医院收治的120例行电视胸腔镜手术治疗的患者作为研究对象,按照信封法分为观察组(n=61)和对照组(n=59)。观察组采用静脉全身麻醉+超声引导菱形肌-肋间肌阻滞麻醉,对照组采用静脉全身麻醉+超声引导TPVB麻醉。比较两组患者手术指标、神经阻滞情况、视觉模拟评分法(VAS)评分、Ramsay镇静评分,统计两组患者并发症发生情况。结果 两组患者手术时间、术中出血量、引流时间、住院时间及首次进食时间比较,差异均无统计学意义(P>0.05)。两组注药时间、阻滞持续时间及补救镇痛次数比较,差异均无统计学意义(P>0.05);观察组超声定位时间、穿刺时间、操作总时间分别为(1.12±0.10)、(2.37±0.56)、(5.47±0.38)min,均短于对照组[(1.65±0.14)、(3.61±0.64)、(6.98±0.42) min],穿刺针深度为(4.43±0.52) mm,小于对照组[(5.37±0.58)mm],差异均有统计学意义(P<0.05)。两组患者术后6、12 h VAS评分、Ramsay镇静评分比较,差异均无统计学意义(P>0.05)。两组患者麻醉后恢复室(PACU)低氧血症发生率、气胸发生率、肺部感染发生率、肺不张发生率、恶心呕吐发生率及总并发症发生率比较,差异均无统计学意义(P>0.05)。结论 与TPVB比较,超声引导菱形肌-肋间肌阻滞应用于电视胸腔镜手术,患者术后恢复质量、镇痛效果及安全性相当,但超声引导菱形肌-肋间肌阻滞操作简单,操作时间更短。

超声引导下RISS阻滞在微创McKeown食管癌根治术后的镇痛效果:一项前瞻性随机对照研究

目的 探讨超声引导下菱形肌-肋间肌-低位前锯肌平面(rhomboid intercostal and subserratus plane, RISS)阻滞对微创McKeown食管癌根治术(minimally invasive McKeown esophagectomy, MIE-McKeown)患者术后镇痛的安全性和有效性,以期为微创食管癌手术患者术后镇痛方案选择提供新思路。方法 前瞻性收集2022年3月—2023年6月于重庆大学附属涪陵医院胸心外科行MIE-McKeown术患者的临床资料,采用随机数字表法将入组患者分为A、B、C 3组:A组采用持续RISS阻滞+患者自控静脉镇痛(patient-controlled intravenous analgesia, PCIA)策略,B组采用单次RISS阻滞+PCIA策略,C组采用单纯PCIA策略。记录并比较各组主要及次要结局指标:(1)镇痛效果[术后2 h、6 h、12 h、24 h、48 h的静息和咳嗽视觉模拟量表(visual analogue scale, VAS)疼痛评分];(2)术后镇痛药物使用情况[术后24 h内舒芬太尼用量、镇痛泵有效按压次数和补救性镇痛追加次数];(3)术后镇痛期间不良反应发生情况(头晕、嗜睡、恶心呕吐、低血压、尿潴留等);(4)术中血流动力学指标[不同时间点平均动脉压(mean arterial pressure, MAP)和心率];(5)镇痛满意度。其中(1)(2)(3)为主要结局指标,(4)(5)为次要结局指标。结果 共96例符合纳入和排除标准的患者入选本研究,A、B、C每组各32例。A组患者术后2 h、6 h、12 h、24 h、48 h静息和咳嗽VAS评分均低于C组,且术后2 h、24 h的静息VAS评分及术后12 h、24 h的咳嗽VAS评分均低于B组;B组患者术后2 h、6 h、12 h的静息VAS评分及2 h、6 h、12 h、24 h、48 h的咳嗽VAS评分均低于C组,差异均具有统计学意义(P均<0.05)。术后24 h内舒芬太尼用量、镇痛泵有效按压次数及补救性镇痛的追加次数在A、B、C 3组之间逐渐增加,差异具有统计学意义(P均<0.001)。C组头晕、恶心呕吐发生率均高于A、B组(P均<0.05)。3组患者在麻醉诱导前(T_(0))、切皮即刻(T_(1))、切皮后5 min(T_(2))、拔管后5 min(T_(3))的MAP和心率差异均无统计学意义(P均>0.05)。A、B、C 3组镇痛满意度依次降低(P<0.05)。结论 超声引导下RISS阻滞可为MIE-McKeown术患者提供良好的术后镇痛,作为多模式镇痛的积极探索,持续RISS镇痛效果更佳,值得临床进一步推广使用。